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游离钙浓度
相关语句
  intracellular free calcium concentration
    Effect of Substance P on Intracellular Free Calcium Concentration in Cultured Human Gingival Fibroblast
    P物质对牙龈成纤维细胞胞内游离钙浓度的影响
短句来源
    Effect of SP on intracellular free calcium concentration in cultured osteoblast
    P物质对体外培养成骨细胞内游离钙浓度的影响
短句来源
    Objective To investigate the effect of SP on intracellular free calcium concentration (Ca 2+ )i in cultured osteoblast Methods Effect of SP on intracellular free calcium was measured using cell culturing and laser scanning confocal microscopy with fluo 3/AM as a fluorescence probe Results After treated with SP(10 -4 mol/L), 70% of the cells responded to it;
    目的 观察P物质对体外培养成骨细胞内游离钙浓度的影响。 方法 采用细胞培养技术 ,以钙离子敏感荧光探针Fluo 3/AM标记细胞 ,激光扫描共聚焦显微镜检测成骨细胞内游离钙浓度的变化情况。
短句来源
    Objective To investigate the effect of SP on intracellular free calcium concentration (Ca 2+ )i in cultured human gingival fibroblast.
    目的 观察P物质对牙龈成纤维细胞内游离钙浓度的影响。
短句来源
  “游离钙浓度”译为未确定词的双语例句
    The cells were maintained in RPMI 1640 containing 10% heat-inactivated fetal bovine serum (FBS), 2 mmol/L L-glutamine, 100 U/ml penicillin, and 100/xg/ml streptomycin at 37癈 in a humidified, 5% CO2 atmosphere.
    对扫描得到的荧光图像进行分析,得到反映细胞内游离钙浓度在SP刺激下动态变化的曲线。 l结果l细胞刺激前荧光值稳定,加入10一7mol/L浓度的SP后发生明显变化,荧光值平均增加约80%。
短句来源
    the intracellular (Ca 2+ )i increased markedly [fluorescence intensity increased by (90±18)%] Conclusions These results suggested that calcium may have a role as a intracellular secondary messenger in the action of SP in osteoblast.
    结果 经 10 -4 mol/LP物质作用后 ,70 %的成骨细胞发生了反应 ,细胞内游离钙浓度明显升高 [荧光值增加 (90± 18) % ]。 结论 钙离子有可能作为细胞内第二信使 ,介导P物质对成骨细胞的调节作用
短句来源
    Results Cells responded to SP (10 -7 mol/L) by the intracellular (Ca 2+ )i increased markedly (fluorescence intensity increased markedly).
    结果 经浓度为 10 -7mol/L的P物质作用后 ,可见人牙龈成纤维细胞内游离钙浓度升高 (荧光值增加 )。
短句来源
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  intracellular free calcium concentration
In this study, the effects of Ca2+, Sr2+ and Ba2+ on growth, viability and intracellular free calcium concentration in a human leukemic T cell line (Jurkat) were investigated.
      
Intracellular free calcium concentration in rat anterior pituitary cells as indicated by fura-2: effect of arginine-vasopressin
      
Therefore we studied the effects of pinacidil, cromakalim, and cicletanine on membrane currents and on the intracellular free calcium concentration ([Ca2+]i) in cultured endothelial cells from porcine aorta.
      
During the incubation period a persistent and steady elevation of intracellular free calcium concentration ([Ca2+]i) could be detected.
      
We suggest that there exists a direct correlation between the intracellular free calcium concentration and the mode of cell death.
      
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In this paper. the free calcium concentration in saliva was measured and evaluated by the neutral carries ion-selective microelectrodes. The statistical results showed thatthere was no significant difference (the groups of all,P>0.05) between children and adult,between male children and female one,between caries-free and caries-active, and between interval of two weeks. The results suggested that caries might be no direct relation to the calcium concentration in children plysiological saliva. Calcium ion-selective...

In this paper. the free calcium concentration in saliva was measured and evaluated by the neutral carries ion-selective microelectrodes. The statistical results showed thatthere was no significant difference (the groups of all,P>0.05) between children and adult,between male children and female one,between caries-free and caries-active, and between interval of two weeks. The results suggested that caries might be no direct relation to the calcium concentration in children plysiological saliva. Calcium ion-selective microelectrodes seemsto be a good method for measuring free calcium in oral micro-sample fluid.

作者采用中性载体Ca2+-ISME对唾液中的生理性游离钙浓度进行测定和分析,对比结果显示:儿童与成人之间、男女儿童之间、无龋组与龋活跃组之间以及间隔两周时间之间的唾液Ca2+浓度均无显著性差异(P均>0.05),提示龋病与儿童的生理性唾液中的钙含量无直接关系。Ca2+-ISME适用于对口腔微量液体中Ca2+的检测分析.

To study the relationship between the abnormal behavior of calcium (Ca 2+ ) and the pathogensis of recurrent aphthous ulceration (RAU) by measuring quantitatively the concentration of platelets' cytosolic free calcium ions ([Ca 2+ ] i) and tissue calcium (W Ca 2+ ), the patients with RAU (n=50) and the healthy volunteers (n=30) were chosen radomly. Then platelets [Ca 2+ ] i was determined in calcium fluorescent indicator Fura2/AM loaded washed platelets by spectrofluorophotometer and tissue W Ca 2+...

To study the relationship between the abnormal behavior of calcium (Ca 2+ ) and the pathogensis of recurrent aphthous ulceration (RAU) by measuring quantitatively the concentration of platelets' cytosolic free calcium ions ([Ca 2+ ] i) and tissue calcium (W Ca 2+ ), the patients with RAU (n=50) and the healthy volunteers (n=30) were chosen radomly. Then platelets [Ca 2+ ] i was determined in calcium fluorescent indicator Fura2/AM loaded washed platelets by spectrofluorophotometer and tissue W Ca 2+ was measured quantitatively by OCPC autoanalyzer method. The results showed that the concentration levels of platelets [Ca 2+ ] i in patients with RAU in attack stage and attackfree stage were significantly higher than that of the control group (P<0.01, P<0.05), and the concentration level of the ulcerous tissue W Ca 2+ was also obviously higher than that of the normal control group (P<0.001). There was a significantly positive correlation between the platelets [Ca 2+ ] i and the ulcerous tissue W Ca 2+ (P<0.01). The results suggested that the influx disorder and overload of Ca 2+ might play an important role in the pathogensis of RAU.

为探讨钙离子(Ca2+)异常活动与复发性阿弗它溃疡(RAU)发生的关系,随机收集50例RAU患者及30例健康志愿者,分别采集外周全血及口腔正常或溃疡粘膜组织,应用Fura-2/AM荧光比值法及邻甲酚酞复合物(OCPC)自动分析法分别检测血小板游离钙浓度([Ca2+]i)及组织钙含量(WCa2+)。结果发现:RAU患者不论发作期还是间歇期血小板[Ca2+]i均显著高于正常对照者(P<0.01;P<0.05);溃疡组织WCa2+明显升高,与正常对照组比较具有极显著性差异(P<0.001);血小板[Ca2+]i与同期溃疡组织WCa2+呈显著正相关(P<0.01)。提示:血小板游离钙及组织钙超载可能在RAU发生中起重要作用

Objective: ①To evaluate the effects of the four implant materials, hydroxyl poly calcium sodium phosphate (HPA), hydroxyapatite (HA), bioglass ceramics (BGC) and titanium (Ti) on the calmodulin (CaM) content of osteoblasts, the concentration of intracellular free calcium and the ATPases activity of cell membrane of rat osteoblasts cultured in vitro; ②To reveal the biological compatibility of the four implant materials and the index to estimate the biological compatibility condition. Methods: The powders of HPA,...

Objective: ①To evaluate the effects of the four implant materials, hydroxyl poly calcium sodium phosphate (HPA), hydroxyapatite (HA), bioglass ceramics (BGC) and titanium (Ti) on the calmodulin (CaM) content of osteoblasts, the concentration of intracellular free calcium and the ATPases activity of cell membrane of rat osteoblasts cultured in vitro; ②To reveal the biological compatibility of the four implant materials and the index to estimate the biological compatibility condition. Methods: The powders of HPA, HA, BGC and Ti were sterilized and made into extracted solutions with 199 culture medium. The isolated osteoblasts, which were taken from three day old sparague Dawley rats′ calvaria, were incubated in 199 culture medium also. Then the osteoblasts were mixed and incubated into the four kinds of extracted solutions of the implant materials. After three days of co incubation, the cells were taken out, and the cellular calmodulin content were measured with radiobiological analysis. The concentration of intracellular free calcium were under fluorescent analysis, and the ATPases activities of cell membrane were estimated with colorimetric analysis. Results: ①Calmodulin content of the osteoblasts co incubated with all the implant materials decreased, and moreove, only that of the Ti group decreased more obviously than that of the Ti and HPA group (P<0.05). ②The concentration of intracellular free calcium of all the co incubated osteoblasts were as normal as that of the control group. ③Ca\+\{2+\} ATPases activities of osteoblasts co incubated with HPA, HA, BGC increased (P<0.05), and Na\++/K\++ ATPases activities of the cells co incubated with HPA, HA were strenghtened (P<0 05). Conclusion: The indexes of cellular calmodulin content,concentration\;of intracellular free calcium and ATPases activity of cell membrane can reflect the biological compatibility of implant materials, and they may be used as indexes of estimating biological compatibility condition.

目的 :研究羟基聚磷酸钙钠 (HPA)、羟基磷灰石 (HA)、生物玻璃陶瓷 (BGC)和纯钛 (Ti) 4种硬组织替代材料对成骨细胞的细胞内游离钙 ([Ca2 + ]i)浓度、钙调素 (CaM)含量以及细胞膜ATP酶活性的影响 ,探讨这 4种材料的生物相容性。方法 :选用SD乳鼠颅顶骨成骨细胞进行体外培养 ,把HPA、HA、BGC和Ti粉分别制成材料浸提液 ,将培养成活的成骨细胞接种于材料浸提液中培养 ,3d后测定成骨细胞内 [Ca2 + ]i 浓度、CaM含量及细胞膜ATP酶活性。结果 :4种材料对细胞内游离钙浓度无不良影响 ,钛对钙调素含量有明显抑制作用 (P <0 0 5 ) ,HPA、HA、BGC 3种材料对细胞膜Ca2 + ATP酶活性有增强作用 (P <0 0 5 ) ,HPA、HA 2种材料对Na+ 、K+ ATP酶活性有增强作用 (P <0 0 5 )。结论 :[Ca2 + ]i 浓度、CaM含量及细胞膜ATP酶活性能够反映不同硬组织替代材料的生物相容性 ,可以为建立和完善评价硬组织替代材料生物相容性的方法和指标提供依据。

 
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