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游离钙浓度     
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  free calcium
    Effect of J-894 on Cytoplasmic Free Calcium Concentration in Human Platelet Activation
    J-894对人体血小板激活时胞浆内游离钙浓度的影响
短句来源
    Effect of meglumine cyclic adenylate on thrombin-induced concentration change of cytosolic free calcium in human platelets
    葡甲胺环腺苷酸对凝血酶诱导的人血小板胞浆游离钙浓度变化的影响
短句来源
    EFFECTS OF 1-(2,6-DIMETHYLPHENOXY)-2-(3,4-DIMETHOXY PHENYLETHYLAMINO)PROPANE HYDROCHLORIDE ON CYTOSOLIC FREE CALCIUM CONCENTRATION OF RABBIT PLATELETS
    1-(2,6-二甲基苯氧基)-2-(3,4-二甲氧基苯乙氨基)丙烷盐酸盐对兔血小板胞浆游离钙浓度的影响
短句来源
    Effects of Rotundine on Cytosolic Free Calcium Concentration in Porcine Pulmonary Artery Smooth Muscle Cells
    颅通定对猪肺动脉平滑肌细胞胞浆游离钙浓度的影响
短句来源
    Nicorandil inhibits ATP-induced cytosolic free calcium increase in cultured vascular smooth muscle cells
    尼可地尔降低血管平滑肌细胞内ATP诱导的游离钙浓度
短句来源
更多       
  free calcium concentration
    Effect of J-894 on Cytoplasmic Free Calcium Concentration in Human Platelet Activation
    J-894对人体血小板激活时胞浆内游离钙浓度的影响
短句来源
    EFFECTS OF 1-(2,6-DIMETHYLPHENOXY)-2-(3,4-DIMETHOXY PHENYLETHYLAMINO)PROPANE HYDROCHLORIDE ON CYTOSOLIC FREE CALCIUM CONCENTRATION OF RABBIT PLATELETS
    1-(2,6-二甲基苯氧基)-2-(3,4-二甲氧基苯乙氨基)丙烷盐酸盐对兔血小板胞浆游离钙浓度的影响
短句来源
    Effects of Rotundine on Cytosolic Free Calcium Concentration in Porcine Pulmonary Artery Smooth Muscle Cells
    颅通定对猪肺动脉平滑肌细胞胞浆游离钙浓度的影响
短句来源
    Effects of trihexyphenidyl on aggregation and intracellular free calcium concentration in human platelets
    苯海索对凝血酶诱导的人血小板聚集和胞浆游离钙浓度的影响
短句来源
    The intracellular free calcium concentration plays a key role as the second messenger in the regulation of cellular reaction. The changes of cytoplasmic free calcium concentration([Ca~(2+)]_i) in human platelets loaded with the fluorescent calcium indicator quin-2 were measured in this study.
    细胞内游离钙浓度在细胞反应的调节中作为第二信使起很重要的作用,本实验采用荧光钙指示剂 quin-2测定血小板胞浆内游离钙浓度[Ca~(2+)]_i 的变化。
短句来源
更多       
  intracellular free calcium concentration
    Effects of trihexyphenidyl on aggregation and intracellular free calcium concentration in human platelets
    苯海索对凝血酶诱导的人血小板聚集和胞浆游离钙浓度的影响
短句来源
    The intracellular free calcium concentration plays a key role as the second messenger in the regulation of cellular reaction. The changes of cytoplasmic free calcium concentration([Ca~(2+)]_i) in human platelets loaded with the fluorescent calcium indicator quin-2 were measured in this study.
    细胞内游离钙浓度在细胞反应的调节中作为第二信使起很重要的作用,本实验采用荧光钙指示剂 quin-2测定血小板胞浆内游离钙浓度[Ca~(2+)]_i 的变化。
短句来源
    The effect of ketotifen (Ket) on intracellular free calcium concentration([Ca~(2+) ] i) was examined in freshly isolated myocardial cells prepared from neonatal rats using the [Ca 2+ ] i-sensitive fluorescence indicator Fura-2/AM.
    观察了酮替芬 (Ket)对新生鼠心肌细胞内游离钙的影响 ,以酶法制备新生大鼠心肌细胞悬液 ,用荧光探针Fura - 2 /AM检测心肌细胞内游离钙浓度的变化。
短句来源
    Effects of flavonoids of Hippophae rhamnoides L on intracellular free calcium concentration in cultured rabbit aortic smooth muscle cells
    醋柳黄酮对血管平滑肌细胞胞内游离钙浓度的影响
短句来源
    Effects of hydrogen peroxide on intracellular free calcium concentration in cultured neonatal rat cardiac myocytes and its antagonism by carvedilol
    过氧化氢对乳鼠心肌细胞内游离钙浓度的影响及卡维地洛的拮抗作用
短句来源
更多       
  free intracellular calcium concentration
    ObjectiveTo analyze the effect of 4-aminopyridine (4-AP) on free intracellular calcium concentration (i) in cultured mouse cortical neurons and explore the possible pharmacological mechanism.
    目的研究4-氨基吡啶(4-AP)对体外培养的皮层神经元细胞内游离钙浓度([Ca2+]i)的影响,了解4-AP的药理学作用机制.
短句来源
    AIM: To observe the effects of methylflavonola-mine ( MFA ) on free intracellular calcium concentration ([Ca2+]|) of isolated embryonic rat brain cells in presence and absence of high extracellular potassium and L-glutamate.
    方法:用钙离子荧光染料Fura 2-AM负载后,测定分离的胎鼠脑细胞内游离钙浓度([Ca~(2+)]_i)及其变化。 结果:在含钙1.3mmoL·L~(-1)的Hanks’液中,[Ca~(2+)]_i为197±20nmol·L~(-1)(n=44)。
短句来源
    METHODS: Animal model was produced by occlusion of four vessels. At the same time, free intracellular calcium concentration was measured by using calcium sensitive fluorescent indicator Fura 2/AM in isolated embryonic rat brain cells.
    方法:用Pulsineli四动脉结扎法造成大鼠急性前脑缺血40min后再灌注1h的损伤模型,同时应用Fura-2/AM荧光指示剂测定硫酸锌对胎鼠脑细胞内游离钙浓度的影响。
短句来源
    AIM: To study the effects of hyperin (Hyp) on free intracellular calcium concentration ([Ca 2+ ] i) of brain cells. METHODS: The neonatal rat brain cells were dissociated.
    目的:研究金丝桃苷(Hyp)对新生大鼠的脑细胞内游离钙浓度的作用.方法:分离新生大鼠的脑细胞;
短句来源

 

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  free calcium
Effect of free calcium ions on the proliferative activity and viability of mouse embryonic stem cells
      
These changes include a decline in the amount of calcium in the bone pool inexchangeable with free calcium and a decrease in the capacity of tissue and blood buffer systems to retain calcium.
      
Involvement of cAMP-Dependent protein kinase and intracellular free calcium ions in regulation of ovulation of the follicle-encl
      
It was concluded that cAMP-dependent protein kinase and intracellular free calcium ions were involved as second messengers in the gonadotropin regulation not only in maturation of amphibian oocytes but also in ovulation.
      
The calcium ion complexing ability of orthophosphates, polyphosphates and citrate was determined by measuring free calcium ions in mixtures of these salts and calcium chloride.
      
更多          
  free calcium concentration
Role of increased cytosolic free calcium concentration in myocardial ischemic injury
      
Increases in cytosolic free calcium concentration ([Ca2+]I) may play an important role in myocardial ischemic injury.
      
The MTX-induced depolarization and the increase in cytosolic free calcium concentration were dependent on extracellular Ca2+ ions.
      
The effects of gonadotropin-releasing hormone (GnRH) and GnRH-associated peptide (GAP) on cytosolic free calcium concentration ([Ca2+]i) were investigated in 20 human nonfunctioning pituitary adenomas.
      
We further investigated the mechanism of the inhibitory effect of inhibin on activin A-induced glycogenolysis, and found that 10-10M inhibin did inhibit the increase in cytoplasmic-free calcium concentration that was seen with 10-9M activin A.
      
更多          
  intracellular free calcium concentration
In this study, the effects of Ca2+, Sr2+ and Ba2+ on growth, viability and intracellular free calcium concentration in a human leukemic T cell line (Jurkat) were investigated.
      
Intracellular free calcium concentration in rat anterior pituitary cells as indicated by fura-2: effect of arginine-vasopressin
      
Therefore we studied the effects of pinacidil, cromakalim, and cicletanine on membrane currents and on the intracellular free calcium concentration ([Ca2+]i) in cultured endothelial cells from porcine aorta.
      
During the incubation period a persistent and steady elevation of intracellular free calcium concentration ([Ca2+]i) could be detected.
      
We suggest that there exists a direct correlation between the intracellular free calcium concentration and the mode of cell death.
      
更多          
  free intracellular calcium concentration
Aequorin measurements indicated that the contractile deficit in the failing myocardium at higher rates of stimulation is associated with decreased free intracellular calcium concentration.
      
TFA significantly reduced the resting and CaCl2-induced increase of free intracellular calcium concentration ([Ca2+]i) in rabbit plateletin vitro.Conclusion: TFA has an antiplatelet effect via the inhibition on the influx of Ca2+.
      
The free intracellular calcium concentration was slightly but significantly diminished in patients compared with healthy volunteers.
      
Specifically, when keratinocytes were exposed to thapsigargin under calcium-depleted culture conditions the subsequent increase in free intracellular calcium concentration was moderate in NF1 keratinocytes compared to controls.
      
Whole-cell patch-clamp recordings of membrane currents and fura-2 measurements of free intracellular calcium concentration ([Ca2+]i) were used to study Mn2+ influx in rat peritoneal mast cells.
      
更多          
  其他


This report is to observe changes of the components in human bile after taking oral bile salts mixture comblned with Glucurolactone and Aspirin, and to probe into the possibility of prevention and treatment of cholesterol calculus. All the T-tube biles were collected from patients operated on for intra-or extra- hepatic gallstones, after the entero-hepatic circle was restored for, at least, three days by clamping T-tube. The results showed: (1) the concentrations of cholesterol, glycoprotein (expressed as Hexosamine)...

This report is to observe changes of the components in human bile after taking oral bile salts mixture comblned with Glucurolactone and Aspirin, and to probe into the possibility of prevention and treatment of cholesterol calculus. All the T-tube biles were collected from patients operated on for intra-or extra- hepatic gallstones, after the entero-hepatic circle was restored for, at least, three days by clamping T-tube. The results showed: (1) the concentrations of cholesterol, glycoprotein (expressed as Hexosamine) and ionized, calcium in bile were decreased significantly: (2) the concentration of bile acid and Lecithin (expressed as total phosphorus) in bile were increased significantly; (3) Cholesterol saturated index was decreased significantly. Thus, we suggest that cholesterol calculus is possible to be prevented and treated by combined medication as above. Moreover, there was very few side-effect and these drugs were very convinent to be obtained with very cheap cost.

本实验是将复方胆酸钠片、肝泰乐、肠溶阿司匹林联合用于肝内外胆管结石术后带“T”管的病人,观察用药后胆汁成分的变化。实验结果表明:用药后胆汁中总胆固醇、氨基已糖、游离钙浓度下降;胆酸、总磷浓度升高;胆固醇饱和度下降,为联合用药防治胆固醇结石提供了实验依据。

Intracellular [Ca+ + ]i was examined using Quin-2. Resting [Ca+ + ]i in platelets loaded with Quin-2 in media containing either 1 mmol/L CaCl2. or 2 mmol/L EGTA was 52.1±5.2nmol/L, 28.4+5.1 nmol/L, respectively. The concentration of external calcium appears to play an important role in cytosolic Cat++ hemeostasis in platelets. Stimulation with A2 3187( 25nmol/L ) caused [Ca++]i to peak at 145±30.8 nmol/L in the presense of extracellular 1mmol/L CaCl2 and 34.4±2.4 nmol/L after addition of 2 mmol/L EGTA. These...

Intracellular [Ca+ + ]i was examined using Quin-2. Resting [Ca+ + ]i in platelets loaded with Quin-2 in media containing either 1 mmol/L CaCl2. or 2 mmol/L EGTA was 52.1±5.2nmol/L, 28.4+5.1 nmol/L, respectively. The concentration of external calcium appears to play an important role in cytosolic Cat++ hemeostasis in platelets. Stimulation with A2 3187( 25nmol/L ) caused [Ca++]i to peak at 145±30.8 nmol/L in the presense of extracellular 1mmol/L CaCl2 and 34.4±2.4 nmol/L after addition of 2 mmol/L EGTA. These data suggested that the most of this rise results from calcium entry across the plasma membrane, with a small contribution to the discharge of intracellular pool of Ca++.

应用Quin-2测得含有1mmol/L CaCl_2和2mmol/L EGTA的介质中血小板内静息游离钙浓度为52.1±5.2nmol/L和28.4±5.1nmol/L。A_(23187)诱导的血小板在有钙或无钙时胞浆游离钙增加的峰值分别为145.8±30.8nmol/L和34.4±2.4nmol/L,山莨菪碱对静息游离钙和无外源钙时A_(23187)刺激的游离钙无影响,山莨菪碱(100μmol/L,25μmol/L)、维拉帕米(10μmol/L)分别降低有外源钙时A_(23187)诱导的游离钙峰值38%、34%、25%。山莨菪碱具有钙拮抗作用。

Twenty-four male Wistar rats were divided into 4 groups and injected subcutaneously with cadmium-metallothionein (CdMT) in the doses of 0, 0.3, 0.6, 0.9 mg Cd / kg respectively. Upon exposure to different dose of CdMT, the dose-dependent increasing of urinary Cd, Ca and protein were observed 12 h after CdMT injection, but there was no change in serum free Ca. In the groups exposed to CdMT, Na+, K+-ATPase (Na pump) and Ca2+-ATPase (Ca pump) activities in kidney cortex were significantly inhibited. A concentration-dependent...

Twenty-four male Wistar rats were divided into 4 groups and injected subcutaneously with cadmium-metallothionein (CdMT) in the doses of 0, 0.3, 0.6, 0.9 mg Cd / kg respectively. Upon exposure to different dose of CdMT, the dose-dependent increasing of urinary Cd, Ca and protein were observed 12 h after CdMT injection, but there was no change in serum free Ca. In the groups exposed to CdMT, Na+, K+-ATPase (Na pump) and Ca2+-ATPase (Ca pump) activities in kidney cortex were significantly inhibited. A concentration-dependent inhibition of Ca pump activity induced by CdCl2 in vitro study was also shown, but it could be protected in the presence of glutathione (GSH) or L-cysteine. A lower content of GSH and a higher content of malondialdehyde (MDA) in kidney cortex were observed in the groups treated with CdMT, as compared with the control group. In the highest dose of CdMT group, cAMP/cGMP ratio was lower than that in the control group. The Ca pump activity, cAMP / cGMP ratio and GSH content in kidney cortex were negatively correlated with the concentration of urinary Ca. The MDA content in kidney cortex was positively correlated with urinary Ca. The results suggested that the changes of Na pump and Ca pump, cyclic nucleotide, GSH, and lipid peroxidation (LPO) induced by CdMT in the kidney would be involved in the disturbance of renal Ca reabsorption which causes calciuria characteristic of the Cd nephrotoxicity.

给雄性Wistar大鼠sc不同剂量的镉金属硫蛋白(CdMT),结果表明镉接触组尿镉、尿钙和尿蛋白浓度都高于对照组,并与镉剂量间存在剂量效应关系,而血清游离钙浓度无变化;镉接触组肾皮质钠泵和钙泵活性低于对照组,体外试验结果也显示镉能抑制钙泵活性,谷胱甘肽(GSH)和半胱氨酸对这种抑制有保护作用}镉接触组肾皮质GSH含量低于对照组,而丙二醛(MDA)含量则高于对照组,高剂量组肾皮质cAMP/cGMP比值低于对照组。肾皮质钙泵活性、cAMP/cGMP比值、GSH含量三者都与尿钙浓度间呈负相关,MDA含量则与尿钙呈正相关,揭示镉引起的钙尿症是由肾重吸收钙障碍造成的,可能与镉引起的肾脏钠泵、钙泵、环核苷酸、GSH和脂质过氧化等改变有关。

 
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