Meanwhile,a preliminary investigation of the optimal fermentation condition,enzyme production regularity and detection system of the activity of xylanase of the bacteria was performed with single factor method,multiple factor combinative method and DNS method,respectively.
(2) The fermentative condition of the recombinant strain was studied. The suitable condition of shocking battle of the strain was as follows. The primary pH of medium was 7.0, the amount of inoculation was 1% and the strain was cultured at 37℃ for 3h, then Hyp was induced to express by 0.5mM IPTG at 30℃ > 120r/min for 8h.
A yield of about 250 mg Trx-sBLyS/g DWC (1686 mg Trx-sBLyS/L) and expression level of about 38.5% in soluble Trx-sBLyS were obtained in a 30-1 bioreactor after optimization of the fermentation conditions.
The influence of fermentation conditions on the component composition of secreted enzyme complexes was revealed.
The characteristic features of enzyme preparations obtained in "cellulase" and "xylanase" fermentation conditions are shown.
Optimization of fermentation conditions for P450 BM-3 monooxygenase production by hybrid design methodology
Sequential methodology based on the application of three types of experimental designs was used to optimize the fermentation conditions for elastase production from mutant strain ZJUEL31410 of Bacillus licheniformis in shaking flask cultures.
Fermentation broth and cell extracts were tested against typed test organisms.
A cellular automata model for simulating fed-batch penicillin fermentation process
The transition rules of CAPFM are designed based on mechanical and structural kinetic models of penicillin batch-fed fermentation processes.
The simulation experimental results show that CAPFM replicates the evolutionary behavior of penicillin batch-fed fermentation processes described by the structured penicillin production kinetic model accordingly.
The forest litter layer can be divided into fresh litter layer (L), fermentation layer (F) and humus layer (H), which may represent different litter decomposition stages.