The optimum fermentation conditions are initial pH 6.5, fermentation temperature 25℃. By using Plackett-Burman design by SAS software, cornstarch . soybean cake and MgSO4·7H2O were chosen as the major components from eight variables.
Optimum fermentation condition of strain 15 was alsodetermined , which indicated that antibiotics of strain 15 had strong inhibition activitywith 28h seed age, 4% inoculation volume (v/v) , 80m1 medium volume , 200 r/minrotatory velocity , 26~C temperature and pH 7.Culture filtrate of 515 mixed with 1% surface active agent had protection effectagainst black spot of cabbage in green house.
In order to optimize the fermentation, through orthogonal tests of carbon and nitrogen source and culture condition tests, we obtain a optimal medium composition(%): cance sugar 0.3, corn flour 0.3, peptone 1.0, soybean meal 0.3, NaCl 0.5, FeSO_4 0.002 and MgSO_4 0.04;
optimum formula for AbM in shaking flask culture was determined, which consist of 3 % of corn starch, 0.8 % of soybean cake meal, and 0.15 % of wheat bran, and its pH was 5.0. The optimum culture conditions are as follows, temperature 30 ℃, velocity of shaking table 120 r/min, fed volume 50 mL/250 mL and culture duration 7 days.
The optimal composition of the medium was obtained and the optimum culture conditions by shaking flask culture were:100 mL medium in 250 mL flask,pH5～6,10% inoculating amount, shaking at temperature of 25℃100 r/min for 8days.
This paper studied the compositions of cultural medium and culture conditions of D2-4 strain againt Botrytis cinerea. On the condition of 28℃,180 rounds per minute,the optimum culture time is 96 hours.
A yield of about 250 mg Trx-sBLyS/g DWC (1686 mg Trx-sBLyS/L) and expression level of about 38.5% in soluble Trx-sBLyS were obtained in a 30-1 bioreactor after optimization of the fermentation conditions.
The influence of fermentation conditions on the component composition of secreted enzyme complexes was revealed.
The characteristic features of enzyme preparations obtained in "cellulase" and "xylanase" fermentation conditions are shown.
Optimization of fermentation conditions for P450 BM-3 monooxygenase production by hybrid design methodology
Sequential methodology based on the application of three types of experimental designs was used to optimize the fermentation conditions for elastase production from mutant strain ZJUEL31410 of Bacillus licheniformis in shaking flask cultures.
Eucalyptus chemithermomechanical pulp (CTMP) was modified with the white-rot fungus 19-6 in a stationary culture condition.
Under the optimum culture condition, the cells of the strain can live for about 18 h.
Depending on the culture condition partial redifferentiation and/or hypertrophy follows.
Fish were exposed to cupric chloride at the concentration of 10 ppb for 4 wk (28 d) in a static culture condition.
Fragments of human adenoid tissue were grown in a tissue culture system where all artificial tissue handling, except for the explanation procedures and tissue culture condition in itself, was excluded.
Here, the expression of VHb, its effect on the growth and antioxidant enzyme status of cells under different culture conditions was studied by cloning the complete regulatory and coding sequences (vgb) for VHb in Enterobacter aerogenes.
The time course of lectin production in culture liquid of the basidial fungus Lentinus edodes strain F-249 in different media under submerged culture conditions was studied.
The obtained data demonstrated a good adaptation of Eiseniella tetraedra to the culture conditions with an artificial organic substrate.
Influence of culture conditions and virulence plasmids on expression of immunoglobulin-binding proteins of Yersinia pseudotuberc
The influence of culture conditions and plasmids on immunoglobulin (Ig)-binding activity of two isogenic strains of Yersinia pseudotuberculosis (plasmid-free strain 48-82- and strain 48+82+ bearing plasmids pYV48 and pVM82) was studied.