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吸收峰
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  absorption peak
    The reaction intermediate was obtained in aqueous-organic two phases and the absorption peak at 710 nm was confirmed to be that of the intermediate in relation to OPDA.
    在水-正丁醇二相体系中提纯得到稳定的中间体,并证实此中间体在710nm处的吸收峰是与OPDA相关的中间体的吸收峰,在-10℃下中间体的半衰期为一个星期。
短句来源
    After cultivation, the removal rate of COD and TOC are 30%and 45%, respectively, and the maximum absorption peak in visible spectrumshifted from 470nm to 4lOnni, and the maximum absorption peak in violetspectrum shifted from 232nm and 250nm to 200nm.
    培养前后,COD去除率为30%,TOC去除率为44.5%,可见光区的最大吸收峰由470nm变为410nm,紫外光区的最大吸收峰由232nm和250nm变为200nm。
短句来源
    The PAS of many persons' blood has absorption peak around the wavelength of 637nm and 664nm.
    许多人的血液的光声谱在637nm和664nm附近有吸收峰
短句来源
    5. Adding the acetyl -CoA to the protein mixture of phaA, phaB and phaC, the mixture was analysed with the wavelength of 230-240nm. The results showed that the absorption peak for PHA could be found at the wavelength of 235nm.
    5.将phaA、phaB和phaC基因表达的蛋白产物混和后,加入底物乙酰CoA,于230nm—240nm波长下,对反应产物进行扫描,发现在波长235nm处有明显的PHA特异吸收峰
短句来源
    The second one is that max absorption peak of amide (II) shifts from 1545cm~(-1) to 1538cm~(-1).
    二是酰胺Ⅱ带最大吸收峰由1545cm~(-1)蓝移至1538 cm~(-1)。
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  “吸收峰”译为未确定词的双语例句
    UV scanning spectrum shows that ProⅠhas absorption at point 280 nm and ProⅡ has absorption at point 280 nm and 675 nm.
    紫外可见光谱显示,ProⅠ在280 nm有一特征吸收峰,ProⅡ在280 nm和675 nm处各有一特征吸收峰
短句来源
    80%EtOH solution 208.60nm(herb and seed), addition to 257.70nm(herb).
    80%乙醇提取液的λmax为208.60nm,全草在257.70nm另有一吸收峰
短句来源
    Cytochrome P450 (P450) is a heme enzyme, whose CO complex gives Soret absorption at 450 nm in its optical absorption spectrum.
    细胞色素P450是一类能与CO结合,形成的复合物在450nm附近具有最大吸收峰的血红蛋白的总称。
短句来源
    Its activity is strongly inhibited by KCN and H2O2 ,which indicated that SOD was CuZn-SOD. The enzyme exhibited one absorption maximum in the ultraviolet at 264.17nm and another in the visible region at 679.14nm.
    经过鉴定,纯化SOD酶受KCN和H2O2的强烈抑制,表明其为CuZn-SOD,紫外与可见光区吸收峰分别为264.17nm和679.14nm。
短句来源
    The native catalase had a molecular mass of 140000 Da and showed the typical Soret band appearing at 408 nm.
    该过氧化氢酶的天然分子量为140000 Da,扫描光谱图在408 nm处出现典型的吸收峰(Soret band)。
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  absorption peak
absorption peak from the electronic spectra is red shifted and the intensity is weakened and that the values of peak current from cyclic voltammetry are decreased significantly in the presence of DNA compared with that in the absence of DNA.
      
The modified enzyme showed an absorption peak at 337 nm and a fluorescent emission peak at 410 nm, which are characteristic of an isoindole derivative formed by OPTA binding to a thiol and an amine group in proximity within the enzyme.
      
The modified enzyme showed an absorption peak at 337 nm and a fluorescent emission peak at 410 nm, which are characteristic of an isoindole derivative formed by OPTA binding to a thiol and an amine group in proximity within the enzyme.
      
A reaction intermediate was obtained in two-phase aqueous-organic system and an absorption peak at 710 nm was confirmed to be that of the intermediate in relation to OPDA.
      
A reaction intermediate was obtained in two-phase aqueous-organic system and an absorption peak at 710 nm was confirmed to be that of the intermediate in relation to OPDA.
      
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(1)Studies of the difference spectra and observations with the visual spectroscope have shown that Brevibacterium ketoglutaricum nov. sp. 2990-6 has a "complete" electron transport chain, including flavoprotein, cytochromes a, b, c and a carbon monoxide sensitive pigment.(2)The absorption peak of the CO sensitive pigment is at 415 mμ and the minimum 440 mμ in the difference spectra.(3)It has been shown that the cytochrome systems of cells harvested from inoculative media are qualitatively similar to those found...

(1)Studies of the difference spectra and observations with the visual spectroscope have shown that Brevibacterium ketoglutaricum nov. sp. 2990-6 has a "complete" electron transport chain, including flavoprotein, cytochromes a, b, c and a carbon monoxide sensitive pigment.(2)The absorption peak of the CO sensitive pigment is at 415 mμ and the minimum 440 mμ in the difference spectra.(3)It has been shown that the cytochrome systems of cells harvested from inoculative media are qualitatively similar to those found in cells taken from a f(?)rmentative media. The former however has a much lower cytochrome content but is richer in the carbon monoxide sensitive pigment.

(1)从差异光谱的观察认为2990-6号菌具有较完整的呼吸链系统,包括细胞色素a,b,c三组以及黄蛋白和烟酰胺腺嘌呤核苷酸。(2)2990-6号菌也具有能与一氧化碳相结合的细胞色素氧化酶。它的一氧化碳差异光谱的吸收峰是415mμ,吸收的最低点在440mμ。(3)比较了在不同生理状况下2990-6号菌的细胞色素含量,结果是发酵菌明显地高于生长菌。

The negative peak at 236mμ of the urea difference spectrum of trypsin was effectively quenched by its specific substrates. A plot of the decrease in the absorbancy difference at 236mμ against the negative logarithm of substrate concentration gives a typical dissociation curve with a pK_s of 4.0. In aqueous solution at pH3.3, the optical rotation of a mixture of trypsin and TAME equalled to the sum of the optical rotation values of trypsin and TAME separately, but in 8M urea at pH3.0 the enzyme showed markedly...

The negative peak at 236mμ of the urea difference spectrum of trypsin was effectively quenched by its specific substrates. A plot of the decrease in the absorbancy difference at 236mμ against the negative logarithm of substrate concentration gives a typical dissociation curve with a pK_s of 4.0. In aqueous solution at pH3.3, the optical rotation of a mixture of trypsin and TAME equalled to the sum of the optical rotation values of trypsin and TAME separately, but in 8M urea at pH3.0 the enzyme showed markedly decreased laevorotation in the presence of TAME than in its absence. These results strongly suggest that even in urea solutions, trypsin combines effectively with its specific substrates and the conformation of trypsin is maintained essentially intact by its substrates.Similar urea difference spectrum was also obtained with DIP-trypsin and the result of substrate quenching of the 236mμ peak of this difference spectrum showed that DIP-trypsin can still combine with TAME. NBS treated trypsin n longer showed distinct urea difference spectrum.The pH difference spectrum of trypsin was very similar to the urea difference spectrum of the enzyme and the relationship between the difference in absorbancy at 236mμ and pH showed a titration curve with a pK of 2.8. In presence of substrate, no pH difference spectrum was observed within the pH range 1.6-3.6. The above results were interpreted to mean that tryptophan residues and carboxyl groups take part, directly or indirectly, in maintaining the secondary and tertiary structure of trypsin, and carboxyl group is probably involved in substrate binding.

胰蛋白酶的专一性底物,对甲苯磺酰-L-精氨酸甲酯,对酶在236mμ的尿素差吸收峰有熄灭作用;不同量的对甲苯磺酰-L-精氨酸甲酯对胰蛋白酶236mμ的尿素差吸收值的影响形成一滴定曲线。在8M尿素溶液中对甲苯磺酰-L-精氨酸甲酯和胰蛋白酶的旋光不具有加和性。这些事实说明在尿素溶液中胰蛋白酶能够和底物结合。二异丙磷酰胰蛋白酶的236mμ尿素差吸收峰亦能被对甲苯磺酰-L-精氨酸甲酯熄灭,指出二异丙磷酰胰蛋白酶仍然具有和对甲苯磺酰-L-精氨酸甲酯结合的能力。用N-溴代琥珀酰亚胺氧化胰蛋白酶的色氨酸残基后,酶的尿素差示光谱消失以及酶的pH差示光谱中反映了一个pK2.8的解离基团,说明色氨酸残基和羧基可能直接或间接与236mμ左右的差示光谱有关。从底物存在下胰蛋白酶在pH1.6—3.6范围内不再呈现pH差示光谱看来,羧基可能参与底物的结合。

A new and simple method for bulk isolation and preparation of serum β-lipoprotein by pure chemical means was presented. By taking advantage of certain properties of the β-lipoprotein-dextran sulfate complex it was found possible to avoid ultracon- trifugation altogether.It was found that macromolecular dextran sulfate could interact specifically at neutral pH with serum β-lipoprotein to form an insoluble complex and that it did not react with serum α-lipoprotein or other serum proteins. The complex formed could...

A new and simple method for bulk isolation and preparation of serum β-lipoprotein by pure chemical means was presented. By taking advantage of certain properties of the β-lipoprotein-dextran sulfate complex it was found possible to avoid ultracon- trifugation altogether.It was found that macromolecular dextran sulfate could interact specifically at neutral pH with serum β-lipoprotein to form an insoluble complex and that it did not react with serum α-lipoprotein or other serum proteins. The complex formed could be rendered soluble by increasing the ionic strength of the medium. The loosely combined macromolecular dextran sulfate was then removed by precipitating it with barium acetate, thus leaving the free β-lipoprotein in solution.The purity and properties of the isolated β-lipoprotein was characterized by means of paper electrophoresis, agar gel electrophoresis and immunoelectrophoretic and ultracentrifugal analyses. It was found that the isolated β-lipoprotein solution no longer containe an appreciable amount of dextran sulfate upon detecting the polyanions with acid polysaccharide dyes. On agar gel electrophoresis there appeared only one spot whose position corresponded to α_2-globulin. On paper electrophoresis there was revealed only one band corresponding to that of β-globulin of whole serum, whether it was stained with protein or lipid dyes. Upon ultracentrifugation with a maximum speed of 50,000 rpm it could float in a high density medium of 1.063g/ml. All the above data suggest that the isolated β-lipoprotein is quite pureUltracentrifugal analysis showed that the lipoprotein contained mainly the β-lipoprotein of S_f 2-6, but there was also a small amount of low density lipoprotein of higher S_f values. Immunoeletrophoretic analysis showed, however, only one precipitating arc with antiwhole serum, indicating that β-lipoproteins of different S_f values may have similar immunochemical properties.The newly prepared β-lipoprotein is transparent and light yellow in color and has 2 to 3sorption peaks in visible light, between 410mμ to 500mμ, and one in ultraviolet light at 275mμ. It can be stored at 2° to 4℃ for 14 days or concentrated at 4℃ without the formation of any precipitate.Finally, the characteristics of dextran sulfate-β-lipoprotein complex in concentrated salt solution was studied by stainning the protein and polyanions with Azo-carmine and Alcian Blue dyes on the same paper strip after electrophoresis. It was found that the complex was still in a state of combination and not at all dissociated.

(1)本文利用大分子D.S.与β脂蛋白复合物的性质对血清β脂蛋白的大量分离及其某些性质进行了研究。(2)自制D.S.可以在中性pH与人血清β脂蛋白形成不溶的复合分子。该复合分子可以溶解于12%NaCl溶液中,并可用纯化学的方法分离,释放自由β脂蛋白。(3)该脂蛋白溶液经纸上电泳、琼脂凝胶电泳及琼脂免疫电泳的鉴定均证实为纯净的β脂蛋白,只有一种免疫性,不再含有可觉察出的D.S.。经超速离心分析,主要含有S,2—6的低密度脂蛋白及少量S_f值较高的β脂蛋白。(4)新鲜的β脂蛋白为草黄色液体,平均浓度为1克/100毫升,在可见光400—500mμ波长有三个明显的吸收峰,在紫外光275mμ波长有一吸收峰。于2℃冰箱保存二周或在4℃浓缩均不致产生沉淀。(5)本文对D.S.-β脂蛋白复合分子的性质进行了初步研究,并对其结合方式作了简单的讨论。(6)本文提供了一个简单、温和、不用长时间超速离心而可大量分离和浓缩血清β脂蛋白的化学方法。

 
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