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Crown gall strains caused by Agrobacterium turncfaciens S-1 702, C-58 have been established from sunflower hypocotyls, tobacco stems and stem pithes,carrot roots and potato tubers. The growth of galls and the appearance of teratomas were investgated. Some crown galls strains changed properties many months after induction. A sunflower crown gall strain changed its shape and growth speed seven months after induction. The teratoma of potato tuber caused by A. tumefaciens S-1 appeared in the early stage of crown... Crown gall strains caused by Agrobacterium turncfaciens S-1 702, C-58 have been established from sunflower hypocotyls, tobacco stems and stem pithes,carrot roots and potato tubers. The growth of galls and the appearance of teratomas were investgated. Some crown galls strains changed properties many months after induction. A sunflower crown gall strain changed its shape and growth speed seven months after induction. The teratoma of potato tuber caused by A. tumefaciens S-1 appeared in the early stage of crown gall induction, but the teratomas arisen from two tobacco unorganized crown gall strains caused by A. turncfaciens 702 about 155 days after induction. Two tobacco crown gall strains caused by different A. tumefaciens strains have different response to phytohomone. Several teratoma strains with different differentiation have been obtained. The reversion of crown galls was investigated by means of induction with phytohormone. Plantlets have been induced from two carrot crown gall strains and two tobacco crown gall strains. All crown gall strains and tcratomas produce nopaline. Nopaline was detected from the tobacco plantlet inducted from teratoma. 用根癌土壤杆菌S-1、702和C-58从向日葵胚轴、烟草茎和茎髓,胡萝卜根及马铃薯块茎诱发并建立了冠瘿株系。观察了它们的生长,畸胎瘤发生。在诱发后数月内有些冠瘿株发生明显变化。向日葵冠瘿株在诱发七个月后外形和生长速度发生了变化。由S-1菌株诱发的马铃薯块茎畸胎瘤发生在诱发初期,但702菌株诱发的两株烟草无组织冠瘿株在诱发后155天左右才出现畸胎瘤,不同菌株诱发的两个烟草冠瘿株对植物激素的反应不同。获得了分化程度不同的一组畸瘤胎。用植物激素研究了冠瘿瘤的逆转。两个胡萝卜和两个烟草冠瘿株系分别得到再生植株。所有冠瘿株包括畸胎瘤产nopaline,由烟草畸胎瘤的再生植株也测出了nopaline。 This paper discusses the course of dedifferentiation and redifferentiation in tissue cuiture of shrualthea(Hibiscus syriacus L.).The results indicate that(1) the calli dedifferentiated from explants are a disorganized system and the estab- lishment of an apical meristem in a highly organized state on calli has to undergo an intermediate state which is in lower degree of organization.This intermediate state in shrubalthea is cambium-like tissue or organized protruding tissue;(2) the cells have different competences... This paper discusses the course of dedifferentiation and redifferentiation in tissue cuiture of shrualthea(Hibiscus syriacus L.).The results indicate that(1) the calli dedifferentiated from explants are a disorganized system and the estab- lishment of an apical meristem in a highly organized state on calli has to undergo an intermediate state which is in lower degree of organization.This intermediate state in shrubalthea is cambium-like tissue or organized protruding tissue;(2) the cells have different competences in accepting the stimuli by exogenous hor- mones and their responses are entirely different even under the same influences of exogenous hormones. 本文从解剖学角度研究了木槿在组织培养中去分化与再分化过程。研究结果表明:(1)由外植体去分化产生的愈伤组织是一种无组织系统,由这种无组织系统产生的高度有组织状态的生长点,其间要经过一种组织化较低的过渡结构——形成层状组织。(2)细胞是以一定的感受态接受激素的影响,因此在一定配比的外源激素影响下,并非所有细胞都发生相同的反应。 This paper, through the in-situ leeward pollution level measurements of the chloroprene discharged from a neoprene workshop at different distances, and through the measurement of disorganized effluence quantity of chloroprene as the calculation analysis of permitted disorganized effluence quantity Qa, proposes that the health protection zone of neoprene production factories should be above 1000 meters. 本文通过对氯丁橡胶生产车间下风向不同距离大气氯丁二烯污染水平的现场测试,对氯丁二烯无组织排放量的测定和允许无组织排放量Q(?)的计算方法分析,提出了氯丁橡胶生产企业的卫生防护距离标准应在1000m以上。
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