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粘附素
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  adhesin
     Effect of Bifidobacterial adhesin on lipopolysaccharide-and H_2O_2-induced proliferation and apoptosis of intestinal epithelial cells in vitro
     双歧杆菌粘附素对脂多糖和H_2O_2调节肠上皮细胞增殖和凋亡的影响
短句来源
     Prokaryotic Expression of P30 Adhesin Gene of Mycoplasma Pneumoniae and Preliminary Application of the rP30 Protein
     肺炎支原体P30粘附素基因的原核表达和初步应用
短句来源
     Conclusions Bifidobacterial adhesin can protect intestinal epithelial cells from the damage by LPS and H2O2, and maintain the balance between the proliferation and apoptosis of the cells.
     结论在体外,双歧杆菌分泌型粘附素能抑制LPS和H2O2对肠上皮细胞的损害作用,维持肠上皮细胞增殖与凋亡的平衡。
短句来源
     Objective To study the effect of Bifidobacterial adhesin on proliferation and apoptosis of intestinal epithelial cell induced by lypopolysaccharide (LPS) and H2O2 in vitro.
     目的研究双歧杆菌分泌型粘附素对脂多糖(LPS)和H2O2体外调节肠上皮细胞增殖和凋亡的影响。
短句来源
     After treatment with Bifidobacterial adhesin, the cell proliferation and apoptosis decreased significantly in LPS group, and in H2O2 group, cell apoptosis was signifi- cantly decreased.
     预先经双歧杆菌分泌型粘附素处理后,LPS组细胞增殖和凋亡率均显著下降,H2O2组细胞凋亡明显减少。
短句来源
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  adhesins
     So far,the discovered adhesins of porcine ETEC have K88(F4), K99(F5), 987P(F6),F41,F42,F165,F17,F18 and so on. Of those,K88,K99,987P and F41 are the most prevalent.
     迄今,在猪源ETEC中发现的粘附素抗原有K88(F4)、K99(F5)、987P(F6)、F41、F42、F165、F17、F18等,而以K88、K99、987P和F41最为流行。
短句来源
     Objective To construct a candidate strain of Helicobacter pylori (Hp) that expresses the proteins of the conservative region of 4 adhesins (BabA2, AlpA, AlpB, and HopZ) and study its immunogenicity.
     目的 构建表达幽门螺杆菌 (Hp) 4种粘附素 (BabA2 、AlpA、AlpB和HopZ)保守区蛋白质的候选菌株 ,并研究其免疫原性。
短句来源
     Therefore,the tetravalent subunit vaccine against piglet colibacillosis was prepared with four different ETEC which contained K88,K99,987P and F41 adhesins,respectively.
     鉴于此,作者选取K88~+、K99~+、987P~+和F41~+株流行粘附素菌株,制备了仔猪大肠杆菌病四价亚单位疫苗,围绕新制品的研制本研究对其实验室试验进行了一些有益的探索。
短句来源
     coli strains for F4 F5 F6 F41 adhesins or other F18 fimbriae negative E coli strains and SalmonellaThe McAbs had been primarily used in slide agglutinations to detect F18 fimbriae antigens of 64 field isolates collected from pigs with postweaning diarrhea and/or edema disease, all of these 64 isolates harboured fed A gene coded the major subunit of F18 fimbriae.
     特异性检测结果显示,上述单抗与表达猪源产F4、F5、F6和F41粘附素菌株、鸡源产Ⅰ型菌毛菌株及沙门氏菌不发生凝集反应。
短句来源
     Monoclonal antibodies(MAbs) reacted specifically with FlSab pili from 107/86, while not with Fl8ac pili from 8199, F4-. F5.. F6~ F41 adhesins from enterotoxigenic E.
     直接凝集试验结果显示:4株单抗只与表达了F18ab菌毛的参考株107/86发生凝集反应,而与表达了F18ac菌毛参考菌株8199(O141ab∶H4)以及猪源产F4、F5、F6和F41粘附素菌株和F18菌毛阴性菌株LC-2α、HB101、TG1及沙门氏菌不发生凝集反应;
短句来源
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  adhesin alpa
     Study on the cloning and expression and the immunogenicity of Helicobacter pylori adhesin AlpA gene
     幽门螺杆菌粘附素AlpA基因克隆、表达及免疫原性研究
短句来源
     Methods The adhesin AlpA DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b(+)and expressed in the BL21(DE3) E. coli strain. Adhesin AlpA immunogenicity was studied by Western blot test.
     方法 利用PCR技术扩增粘附素AlpA基因 ,将其定向插入 pET 2 2b(+)载体 ,在BL2 1(DE3)大肠杆菌中表达并通过免疫印迹实验研究其免疫原性。
短句来源
     Results DNA sequence analysis showed that the sequence of adhesin AlpA DNA was almost the same as that of the published by GenBank. The adhesin AlpA recombinant protein accounted for 31.9% of the total bacterial protein. Western blot analysis of rAlpA confirmed that it could be specially recognized by serum from rabbit immunized with AlpA itself and H.pylori infected patients.
     结果 克隆的粘附素AlpA基因序列与基因库公布的基本一致 ,粘附素AlpA重组蛋白表达量占菌体总蛋白的 31.9% ,经免疫印迹证实该重组蛋白可被AlpA免疫兔血清和Hp感染患者血清所识别。
短句来源
  “粘附素”译为未确定词的双语例句
     Conditions of reaction were as follows: The optimal coating concentration of K88, K99, 987P and F41 antigens was 1∶400,1∶80,1∶40 and 1∶40, respectively. The optimal dilution of corresponding serum samples was 1∶400, 1∶200, 1∶200 and 1∶200, respectively.
     初步确定了各种反应条件: K88、K99、987P、F41粘附素抗原最适包被浓度依次为1∶400、1∶80、1∶40、1∶40,相应粘附素抗体最佳稀释度依次为1∶400、1∶200、1∶200、1∶200。
短句来源
     The Significance of the Expression of Mucins (MUC1、MUC2、MUC5AC) and E-Cadherin in Gastric Carcinomas
     MUC1、MUC2、MUC5AC和E-钙粘附素在胃癌中的表达及意义
短句来源
     Significance on expression of CD44v6 and E-cadherin in hepatocellular carcinoma
     肝细胞癌CD44v6和E-上皮钙粘附素表达意义
短句来源
     Prokaryotic Expression of Helicobacter pylori Adhesion A (hpa A) and Cholerae Toxin B(ctx B)Fusion Gene
     幽门螺杆菌粘附素hpa A和霍乱毒素B亚单位ctx B融合基因的原核表达
短句来源
     The expression of CD_(15S) antigen and E-cadherin in relation to the lung cancer
     CD_(15S)抗原和E-上皮钙粘附素表达与肺癌的关系
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  adhesin
Transformation of a Fragment of β-Structural Bacteriophage T4 Adhesin to Stable α-Helical Trimer
      
Gene product 12 of bacteriophage T4, adhesin, serves to adhere the virus to host cells.
      
Adhesin is a fibrous homotrimer, and a novel tertiary structure element, a β-helix, is supposed to be a major structural feature of this protein.
      
One of these factors, adhesin, which is responsible for cell adhesion, was found to be a protein substance located both at the cell surface and in the CL.
      
Some properties of thePseudomonas fluorescens adhesin and antiadhesin
      
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  adhesins
The hypothesis is discussed that the short-term reversible adhesion of cells is regulated with the involvement of a mixture of hydrocarbons, which inactivate the functional activity of bacterial adhesins, and proteases, which digest these adhesins.
      
The attachment of group A streptococci to oropharyngeal epithelial cells is mediated by adhesive molecules (adhesins) on the surfaces of the micro-organisms that interact with receptor molecules on the epithelial cells.
      
coli strains with genetically defined adhesins support this notion.
      
In contrast to the isolates 536 (O6:K15) and RZ 475 (O6:K5) the strain DSM 6601, belonging to serotype O6:K5:H1, produces neither toxins nor mannose-resistant hemagglutinating (MRHA) adhesins.
      
The most promising candidates to date include adhesins (fibronectinbinding protein, collagen-binding protein, and fibrinogenbinding protein [clumping factor]), a nontoxic alpha toxin mutant, and capsular polysaccharides type 5 and 8.
      
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Seven out of 44 monoclonal antibodies (MAbs) with specificity to K88. K99. F41 and 987P antigens of enterotoxigenic escherichia coli. (ETEC) developed in our laboratory were selected to make up MAbs based diagnostic reagents, which were used for the detection of adhesins mentioned above. The diagnostic system which was characterized by 4 MAbs sandwich ELISA and 3 MAbs sandwich ELISA was also developed. The diagnostic system and protocals were evaluated for the detection of ETEC adhesins in specimens from 1038...

Seven out of 44 monoclonal antibodies (MAbs) with specificity to K88. K99. F41 and 987P antigens of enterotoxigenic escherichia coli. (ETEC) developed in our laboratory were selected to make up MAbs based diagnostic reagents, which were used for the detection of adhesins mentioned above. The diagnostic system which was characterized by 4 MAbs sandwich ELISA and 3 MAbs sandwich ELISA was also developed. The diagnostic system and protocals were evaluated for the detection of ETEC adhesins in specimens from 1038 natural cases of pig scour. It was confirmed the diagnostic system and protocals developed in this study had the advantages of sensitivity, accuracy, rapidity, and simplicity.

作者从所研制的抗产肠毒素性大肠埃希氏菌(ETEC)粘附素K_(88)、K_(99)、987P和F_(41)单克隆抗体(以下简称单抗)44株中的7株,建立了检测以上粘附素抗原的单抗诊断试剂,并确定了以4单抗和3单抗夹心ELISA为特征的检测大批量临床样品的诊断方法与程序,对1038例自然发生下痢仔猪粪样ETEC粘附素的检测结果表明,本试验所建立的诊断方法与程序,具有敏感、准确、快速和简便的特点。

An enterotoxigenic Escherichia coli strain isolated from a piglet with diarrhea was examined for the presence of fimbriea 987P and F41 by a direct agglutination (with MAbs),an indirect immunofluorecence technique (MAbs as first antibodies), SDS-PAGE and Western blots (antisera IgG as probes). Results of these techniues revealed that both 987P and F41 fimbrial adhesins were produced by the same strain, not by separate ones.

通过直接凝集试验、免疫荧光试验、SDS-PAGE和Western印迹,对一株猪源性大肠杆菌的粘附素进行了研究.结果表明,该菌株是一株同时表达987P和F_(41)两种粘附素抗原的猪源性大肠杆菌.

Abstract The human parotid saliva was separated by anionic polyacrylamide gel electrophoresis(PAGE)and seven bands of salivary proteins named B1~B7 were purified,in order to find out the acceptors of Streptococcus mutans MT6R(serotype c)adhesin P1,the adhesion of adhesin P1 labbelled with 131I(131 I-P1)to the different pellicles of seven purified salivary proteins were studied.The results showed that the 131I-P1 selectively adhere to the salivary protein components.The protein B5 was most effective in promoting...

Abstract The human parotid saliva was separated by anionic polyacrylamide gel electrophoresis(PAGE)and seven bands of salivary proteins named B1~B7 were purified,in order to find out the acceptors of Streptococcus mutans MT6R(serotype c)adhesin P1,the adhesion of adhesin P1 labbelled with 131I(131 I-P1)to the different pellicles of seven purified salivary proteins were studied.The results showed that the 131I-P1 selectively adhere to the salivary protein components.The protein B5 was most effective in promoting attachment of 131I-P1 (P<0.01) and protein B5,protein B7 were less effective than protein B5,but more effective than the rest proteins(P<0.01).It has been demonstrated that protein B5 and B6 were the acidic proline-rich proteins.Therefore,acidic proline-rich proteins may be one of the most important acceptors of S.mutans MT6R(serotype c)adhesin P1.

收集人刺激性腮腺唾液,在碱性条件下聚丙烯酰胺凝胶电泳纯化唾液蛋白7种,命名为B1~B7,分别包被羟磷灰石珠,用131碘标记纯化的变形谜球菌MT6R(血清型c)粘附素蛋白P1,观察其对每种唾液蛋白的粘附能力,以研究变形链珠菌粘附素蛋白P1的接受器。结果显示变形链球菌mutans表面蛋白P1对不同唾液蛋白的粘附量不同,具有选择性(P<0.01),对B5,B6粘附量特别高。已证实B5,B6为酸性富脯蛋白,提示酸性富脯蛋白可能为变形链球菌粘附素蛋白P1的重要接受器之一。

 
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