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杂交细胞系
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  hybrid cell lines
     In this study,based on the information of five human genes(FMR1,IDS,FATE,BGN,F8A) on the X chromosome,the linkage relationship of these five genes in pigs were analyzed by a panel of 96 radiation hybrid cell lines.
     根据人类基因组X染色体上FMR1I、DS、FATE、BGN、F8A等5个基因的信息资源,用已构建的猪/仓鼠96个放射杂交细胞系分析猪染色体该5个基因间的连锁关系。
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     Methods Chromosome locus was defined by radiation hybrid mapping method and protein localization by green fluorescence protein(GFP) as reporter molecule.
     方法 应用辐射杂交细胞系 (RH)定位的方法进行染色体定位 ,应用绿色荧光蛋白 (GFP)基因作为报告基因 ,结合荧光显微摄影进行蛋白质定位。
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     Objective To investigate the effects of melatonin on okadaic acid(OA)-induced tau hyperphosphorylation in NG108-15 cell.
     目的观察褪黑素(MLT)对冈田酸(OA)致大鼠神经母细胞和小鼠胶质细胞瘤细胞的杂交细胞系NG108-15细胞tau蛋白过度磷酸化的影响。
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     The gene was located at chromosome 9q22 through RH (radiation hybird) mapping.
     用放射杂交细胞系 (RadialhybridRH)方法定位此基因在人染色体的 9q2 2上 .
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     ECRG 4 gene was located in 2q14.1—14.3 by HTGS and STS, and was conformed by radiation hybrid (RH) method.
     采用辐射杂交细胞系GeneBridge 4RH嵌板作染色体定位进行初步验证 ,所得结果与网上克隆完全一致。
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     Seven novel genes, which were isolated from human fetal liver (22weeks) cDNA library, were successfully mapped to their appropriate chromosomal positio ns by using radiation hybrid (RH) panel. The novel gene HPO was mapped to Chr.
     辐射杂交细胞系(RH)技术是一种体细胞遗传学技术,是继荧光原位杂交技术(FISH)后新建立的染色体定位方法。 本文应用该技术将发现于22周孕龄人胎肝的7个新基因进行了染色体定位:肝细胞生成素(hepatopoietin,HPO)定位于Chr.
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     The gastric MALToma is the most common extranodal lymphoma,and they are of B cell origin.
     瘤细胞B细胞来源。
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     Scanning the Differential Methylation Sequences in Hepatocellular Carci-noma Cell Line by Subtractive Hybridization
     结合消减杂交筛选肝癌细胞中差异甲基化位点
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     Cell hybridization techniques and of their some applications.
     细胞杂交技术及其应用
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     2. COS7 cell line
     2.COS7细胞
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     Expression of miR-28 in B Cell Lymphoma Cell Lines Detected by Solution Hybridization
     液相杂交检测B细胞淋巴瘤细胞miR-28的表达
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  hybrid cell lines
Cytogenetic and immunological study of mammalian hybrid cell lines
      
Two hybrid cell lines, KS-RL-3 (hybrid between TK- sheep kidney cells and rabbit lymphocytes) and CR-KS TK- (hybrid between rabbit β-cells and TK- sheep kidney cells), were assayed cytogenetically.
      
It was shown that these hybrid cell lines were characterized by the presence of both sheep and rabbit chromosomes, with a number and structure which varied depending on the cell type and the number of passages.
      
Partial immunological tolerance of the hybrid cell lines was suggested.
      
This hypothesis could explain why CD4+ cells could not be rendered permissive to HIV entry by transfection of human DNA, or by forming stable hybrid cell lines bearing human chromosomes.
      
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  hybrid cell line
The RH panel was obtained on the basis of a hybrid cell line bearing the single porcine chromosome 2 against the background of mink chromosomes.
      
A positive hybrid cell line secreting antibodies against the free α-subunit of hCG was cloned twice by limiting dilution method and eighty four clones were obtained that secreted monoclonal antibodies anti-αhCG.
      
Expression of human coagulation factor VIII in a human hybrid cell line, HKB11
      
To understand cellular factors limiting FVIII expression, we studied the expression of FVIII in a human cell line, HKB11 (a hybrid cell line of HEK293 and a human B cell line).
      
The genetic instability of an intertribal hybrid cell line, Duboisia hopwoodii + Nicotiana tabacum, obtained by mechanical isolation of a single hybrid cell was studied.
      
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Neuroblastoma×glioma hybrid cell line, NG108-15 cell formed by the fusion of mouse neuroblastoma and rat glioma expresses many properties of mature neurons after being differentiated by N6O2-dibutyryladenosine 3', 5'-cyclic monophosphats (dbcAMP) or prostaglandin E1 (PGE1). The differentiated NG108-15cells display a wide range of ion channels and receptors found in neural cells and are being used widely in neurobiologcal researches. This paper describes the ion-channels characterized at NG108-15 cell and their...

Neuroblastoma×glioma hybrid cell line, NG108-15 cell formed by the fusion of mouse neuroblastoma and rat glioma expresses many properties of mature neurons after being differentiated by N6O2-dibutyryladenosine 3', 5'-cyclic monophosphats (dbcAMP) or prostaglandin E1 (PGE1). The differentiated NG108-15cells display a wide range of ion channels and receptors found in neural cells and are being used widely in neurobiologcal researches. This paper describes the ion-channels characterized at NG108-15 cell and their properties aswell as some examples of using the cell line to Study the actions of bioactive subeboces.

由神经母细胞瘤和神经胶质细胞瘤融合杂交的细胞系NG108-15细胞(neuroblastoma×gliomahybridcell),经分化后具成熟神经细胞的特性,存在多种在神经细胞膜发现的离子通道和受体,现已被作为神经细胞模型广泛地应用于神经生物学研究。本文就已在该细胞膜上发现的离子通道的种类及其性质做了介绍,并列举实例,说明它在分析生物活性物质作用机制中的应用。

A novel RING finger gene was isolated from screening a human fetal brain cDNA library. In additional, two bipartite nuclear localization signals(NLS BP) were found in its protein sequence. The gene was located at chromosome 9q22 through RH (radiation hybird) mapping. The result of Northern blot using MTN (multi tissue Northern) indicated that it expressed in testis at very high level however, at very low lever in prostate and ovary. In situ hybridization of mouse testis indicated that the mouse homology...

A novel RING finger gene was isolated from screening a human fetal brain cDNA library. In additional, two bipartite nuclear localization signals(NLS BP) were found in its protein sequence. The gene was located at chromosome 9q22 through RH (radiation hybird) mapping. The result of Northern blot using MTN (multi tissue Northern) indicated that it expressed in testis at very high level however, at very low lever in prostate and ovary. In situ hybridization of mouse testis indicated that the mouse homology of the RING finger gene was expressed high in spermatogonia and primary spermatocyte. These suggested that the RING finger gene was important in spermatogenesis.

从人胎脑构建的cDNA文库中 ,通过大规模测序筛选的方法获得一条新基因 .该基因蛋白序列含有 1个环指 (RINGfinger)基序及 2个进核信号 (nuclearlocalizationsignal,NLS)序列 ,将此基因命名为RNF2 0 (RINGFinger 2 0 ) .用放射杂交细胞系 (RadialhybridRH)方法定位此基因在人染色体的 9q2 2上 .Northern杂交表明 ,其mRNA在睾丸组织中高表达 .用小鼠睾丸做原位杂交表明 ,小鼠的同源基因在精原细胞及初级精母细胞中高表达 ,未成年小鼠睾丸中的精原细胞没有表达 ,提示RNF2 0可能参与精子发生的调控作用 .

Using Internet as platform, databases as materials and software as tools to assemble a lab on line is revolutionizing in bioscience research. The major works of lab on line are cloning, identification, localization of genes, and the structural and functional analysis of proteins. In this report, the esophageal cancer related gene 4( ECRG 4 )(accession number: AF325503) was successfully isolated. The 97 bp ECRG 4 EST was initially used to fish the human EST databases. Five pieces of ESTs showed...

Using Internet as platform, databases as materials and software as tools to assemble a lab on line is revolutionizing in bioscience research. The major works of lab on line are cloning, identification, localization of genes, and the structural and functional analysis of proteins. In this report, the esophageal cancer related gene 4( ECRG 4 )(accession number: AF325503) was successfully isolated. The 97 bp ECRG 4 EST was initially used to fish the human EST databases. Five pieces of ESTs showed strong homology to it, and they were assembled to one 772 bp cDNA sequence by DNASTAR software. Then the 447 bp full open reading frame of ECRG 4 was determined by ORF FINDER to encode 148 amino acids. Sequence of ECRG 4 did not reveal remarkable similarity to the known sequences in a homology analysis with the public database of GenBank, showing that it is a new gene. Homology analysis of protein coding by ECRG 4 showed a 31% homology with mouse IgG V region. ECRG 4 gene is expressed in normal esophagus, bladder and brain tissues, but its expression was significantly down regulated in prostate tumors and tumor cell lines. ECRG 4 gene was located in 2q14.1—14.3 by HTGS and STS, and was conformed by radiation hybrid (RH) method. We propose that this purely lab on line cloning approach can be used by modestly sized laboratories to rapidly and accurately characterize human genes without wasting too much money and time.

利用生物信息学 ,探索网上克隆基因与鉴定基因的新方法。以因特网为平台 ,数据库为试验材料 ,各种软件为工具组成网上实验室 ,是人类基因组计划带来的实验技术革命。利用网上实验室以食管癌相关基因E CRG 4的 97bpEST为基础 ,成功克隆并鉴定了该基因。结果显示ECRG 4近似cDNA全长序列为 772bp ,其中含有一 44 7bp的完整阅读框 ,编码 148个氨基酸。氨基酸序列相似性分析表明ECRG 4与细胞膜上的免疫球蛋白超家族具有 31%同源性。该基因定位于染色体 2 q14.1~ 14.3。组织分布表明ECRG 4在正常食管、膀胱组织中表达明显高于相应的癌组织。采用辐射杂交细胞系GeneBridge 4RH嵌板作染色体定位进行初步验证 ,所得结果与网上克隆完全一致。研究提示 ,利用网上实验室克隆鉴定基因 ,是一种简便、精确的好方法。ECRG 4可能是一个在细胞癌变过程中具有非常重要意义的基因

 
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