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凋亡率
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  apoptosis rate
    Result: The apoptosis rate decreased obviously after reparation and intervention,the result of Western blot showed that activated caspase-3 decreased obviously in all reparative groups,furthermore,the express of c-IPI-1,c-IPI-2 increased in all reparative groups,the 15% serum group was most obvious in all changes.
    结果:修复干预后的凋亡率有明显下降,W estern b lot结果显示各修复组活化的caspase-3明显减弱,并且各修复组的c-IPI-1,c-IPI-2的表达增强,其中各种变化都以15%血清组的变化最明显。
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    On days 3, 7 and 14 after irradiation, the expressions of VEGF and Flt-1 in murine bone marrow stromal cells were assayed by immunohistochemical method. The apoptosis rate of bone marrow stromal cells was tested by flow cytometry.
    在照射后第3,7,14天采用免疫组化法检测小鼠骨髓基质细胞血管内皮生长因子及其受体Flt-1的表达,并用流式细胞术检测相应时间的骨髓基质细胞凋亡率
短句来源
    Results: (1) Apoptosis rate in HA group (92.0±2.6) was significantly higher than that in control group (3.5 ± 1.8).
    结果:H202组LEC凋亡率(92.0±2.6)显著高于空白对照组(3.5±1.8),(P<0.01);
    Apoptosis rate in Sch B group (13.8±3.27) was remarkably lower than that in HA group and PS group .
    Sch B组LEC凋亡率(13.8±3.0)显著低于H202组,且与PS组(56.0±9.9)有显著的差异(P<0.05)。
    (5)The PGLH7 cells apoptosis rate treated with 10% high dose drug-bearing serum at 24 h, was significantly higher than that of the control serum (P<0.05) by flow cytometric assay.
    (5)采用Annexin V-FITC和PI双标记,流式细胞仪定量检测PGLH7细胞凋亡率,莪术瓜篓汤含药血清作用24h时,高剂量血清组与对照血清组之间的凋亡率差异有统计学意义(P<0.05);
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  apoptotic rate
    ResultsAfter being interfered by hypoxia for 24 h,the levels of reactive oxygen species(ROS)in HUVECs and the apoptotic rate either in the early or in the late stage significantly increased,and accompanied with the increased levels of endothelin-1 mRNA(ET-1)and endothelial oxide synthase(eNOS)protein.
    结果缺氧干预内皮细胞24h后,细胞活性氧(reactive oxygen species,ROS)水平及早期、晚期凋亡率均明显增加,伴随内皮素(endothelin-1,ET-1)mRNA水平及内皮型一氧化氮合成酶(endothelial oxidesynthase,eNOS)蛋白水平的升高。
短句来源
    However,when HUVECs were pretreated with GBE50(25 μg/ml)4 h before hypoxia,the apoptotic rate in the early or late stage and expression of ET-1 mRNA significantly decreased(P<0.05),and the heightened ROS level and eNOS expression partially decreased(P>0.05).
    缺氧前4h给予GBE50干预后发现,GBE5025μg/ml时可显著降低细胞早期及晚期凋亡率及ET-1mRNA水平(P<0.05),部分降低因缺氧导致的ROS水平及eNOS蛋白表达的增高(P>0.05)。
短句来源
    (3)There were significant difference of the concentration of [Ca2+]i and the apoptotic rate between the drugs and control group.
    (3)治疗各组细胞内[Ca~(2+)]i和凋亡率显著升高,与模型组相比较有显著差异(P<0.001)。
短句来源
    The apoptotic rate of each group was measured by flow cytometry. Results: The apoptotic rate of control group(0.8% ± 1.02% ) was lower than ischemia group(13.8% ±1.14%);
    结果:正常对照组凋亡率很低(0.8%±1.02%),缺血对照组的凋亡率显著增高(13.8%±1.41%),活血组(10.2%±1.34%).
短句来源
    The apoptotic rate of HUVEC tes ted by PI staining increased from (7.1±0.7)% to (38.1±4.0)% aft er 18 h of trea tment with H 2O 2. Salvianolic acids 100 mg·L -1 reduced the apoptotic rate to (13.6±0.8)%.
    1 0 0 μmol·L-1 H2 O2 还可引起内皮细胞的凋亡 ,PI染色的结果显示 ,H2 O2 处理内皮细胞 1 8h后 ,凋亡率从 (7.1 %± 0 .7) %升至 (38.1±4.0 ) %。
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  ratio of apoptosis
    Results:The ratio of apoptosis in MNC of cord blood decreased when ginsenosides were put into the culture. Ginsenosides suppressed the apoptosis in a dose-dependent manner. PMA,a PKC activator,also suppressed the effect of ginsenosides.
    结果 :人参皂甙对脐血单个核细胞的凋亡有抑制作用 ,且凋亡率与其浓度呈负相关 ,PKC激活剂佛波酯 (PMA)单独作用也可抑制细胞凋亡作用 ,人参皂甙抑制脐血细胞凋亡的作用可被PKC抑制剂H7 抑制 ,此作用随H7 浓度增加而增大。
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  “凋亡率”译为未确定词的双语例句
    Results The rate of apoptosis and the Fas/FasL expression of ASMCs in the equisetum treating group were both higher than those of the model group(P< 0.01,P<0.05),and the degree of atherosclerotic lesion was less than that of the model group.
    结果木贼正丁醇提取物治疗组平滑肌细胞的凋亡率明显高于模型组(P<0.01),Fas、FasL表达均高于模型组(P<0.01及P<0.05),主动脉损伤程度减轻。
短句来源
    Results The medicated serums has significant effects of promoting HSC-T6 apoptosis at these two points of time.
    结果2h、3h两个时间点的含药血清能明显促进HSC-T6细胞的凋亡,其中2h2%含药血清的促凋亡率5.71%,3h2%含药血清的促凋亡率5.73%。
短句来源
    Compared with control group, apoptotic cell numbers of the apoptotic outer nuclear layer cells were obviously reduced after 7,14,21,28 d and 35 d, and the Bcl-2 expression in the matrix of retinal photoreceptors and photoreceptor outer segments were obviously increased after 3, 7,14,21,28 d and 35 d in experimental group in rd mice.
    rd小鼠经葛根素药物治疗后d7、14、21、28、35,光感受器细胞的凋亡率比未用药的对照组明显减少,P<0.01; d3、7、14、21、28、35,Bcl-2在视网膜外核层及光感受器细胞外段的表达明显增强,P<0.05或P<0.01。
短句来源
    Results The NO contend of group A and group B was(50.00±30.04)μmol·L-1and(83.30±16.20)μmol·L-1respectively,the apoptosis percentage was(38.18±13.49)%and(74.80±9.37)%respectively,there was significant difference between the two groups.
    L-1和(83.30±16.20)μmol. L-1,软骨细胞的凋亡率分别为(38.18±13.49)%和(74.80±9.37)%。
短句来源
    Myocardial cell apoptosis was only detected in MC and Sen group, with the apoptosis index (AI) of (15.686 0±0.688 7)% and (5.579 0±0.191 7)% respectively (P<0.05).
    对照组未见TUNEL阳性细胞,MC组和Sen组心肌细胞凋亡指数(AI)分别为(15.6860±0.6887)%和(5.5790±0.1917)%(P<0.05),凋亡率分别为(6.9320±0.6380)%和(3.0210±0.2540)%(P<0.05);
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  apoptosis rate
Only NPSLE patients exhibited an increased neglect-apoptosis rate after incubation with culture medium; however, the neglect-apoptosis rate was associated with lymphopenia in both series of patients.
      
After lymphocyte incubation with autologous serum, only NPSLE patients exhibited a significant negative correlation between the neglect-apoptosis rate and the number of peripheral lymphocytes.
      
The total apoptosis rate is positively proportional to reaction duration and concentration of quercetin used.
      
The maximum apoptosis rate being (88.76±2.35)% was obtained when the concentration was 50.0 μmol/L and the cells were treated with quercetin for 72 hours.
      
The highest apoptosis rate was found to reach 13.38% by flow cytometry at 6 h after EMP irradiation.
      
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  apoptotic rate
When the concentration of thrombin is 40 U/mL, TUNEL positive cells and apoptotic rate of hippocampal neurons reached peak value, were 27.3±4.0 and (29.333±4.633)%, respectively.
      
AS PS-ODNs increased the chemotherapy sensitivity and apoptotic rate.
      
Typical nuclear condensation and apoptosis bodies were observed as early as 24 hrs after a 0.5 ?g/ml Taxol treatment; Apoptotic rate of the Taxol-treated K562 cells increased from 3.7% to 24.0% in 24 hrs.
      
After irradiation and addition of curcumin, the expression of hMSH2 mRNA increased and the cellular apoptotic rate also increased at the same time.
      
Apoptotic rate was quantified by flow cytometry (FCM).
      
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  ratio of apoptosis
Induced for 18 h by CD34+ cells transfected with FasL or without, the ratio of apoptosis of U937 cells was (5.0±1.3)%, (10.8±0.6)% (P>amp;lt;0.01), respectively.
      
The ratio of apoptosis of T cells was 12.1±1.5% when CD34+ cells transfected with FasL was used as stimulus cells, much higher than that of CD34+ cells non-transfected (3.2±1.1%,P>amp;lt;0.01).
      
The ratio of apoptosis to mitosis was calculated because it can give a better indication of any shift in the dynamic status of the tumors.
      
The ratio of apoptosis to PCNA appears to be a marker that predicts response to this therapy.
      


Objective:To explore the effect of tripterine in inducing human mast cell line(HMC-1)cell apoptosis.Methods: Cells were exposure to tripterine(0.125-1.0 μmol/L)for different time and assayed by DNA electrophoresis and flow- cytometry.Results:(1)Apoptosis of HMC-1 cells could be efficently induced by tripterine with the presence of apoptotic change in morphology.DNA ladder on argrose gel electrophoresis and apoptotic peak before G_1 phase of cell cycle on flow- cytometry;(2)The magnitude of apoptosis increased...

Objective:To explore the effect of tripterine in inducing human mast cell line(HMC-1)cell apoptosis.Methods: Cells were exposure to tripterine(0.125-1.0 μmol/L)for different time and assayed by DNA electrophoresis and flow- cytometry.Results:(1)Apoptosis of HMC-1 cells could be efficently induced by tripterine with the presence of apoptotic change in morphology.DNA ladder on argrose gel electrophoresis and apoptotic peak before G_1 phase of cell cycle on flow- cytometry;(2)The magnitude of apoptosis increased with the augmentation of tripterine concentration and duration of exposure;(3)With diminution of Sphase cells,apoptotic cells increased.They bore significant negative relation(P<0. 01).Conclusion:The results suggested that tripterine could efficiently induce HMC-1 cell apoptosis,occurring mainly in S phase.

目的:研究雷公藤红素对人肥大细胞系 HMC-1细胞凋亡的诱导作用。方法:HMC-1细胞与雷公藤红素(0.125~1.0μmol/L)共育不同时间,经 DNA 琼脂糖电泳和流式细胞仪 DNA 含量分析。结果:(1)雷公藤红素能有效地诱导 HMC-1细胞凋亡,细胞呈凋亡形态学改变,DNA 琼脂糖电泳出现梯形条带,流式细胞仪分析,在 G_1期峰前出现凋亡峰。(2)凋亡程度随药物浓度增加和作用时间的延长而提高。(3)凋亡率随 S 期细胞比例的下降而升高,二者呈显著负相关(P<0.01)。结论:雷公藤红素可以诱导 HMC-1细胞凋亡,凋亡主要发生在 S期。

Objective: Approach to the effect of composed Chinese drug 892 on inducing the apoptosis of SPC A1. Methods: According to the basic TCM theory, the pathologic characteristic of lung cancer is stagnation of phlegm and turbid qi . Drug 892, composed of Banxia (Rhizoma Pinelliae), Tiannanxing (Rhizoma Arisaematis), and Sanqi (Radix Notoginseng). After incubating SPC A1 and drug-bearing rabit serum which at different concentration with serologic pharmacological method, observed and detected the growth...

Objective: Approach to the effect of composed Chinese drug 892 on inducing the apoptosis of SPC A1. Methods: According to the basic TCM theory, the pathologic characteristic of lung cancer is stagnation of phlegm and turbid qi . Drug 892, composed of Banxia (Rhizoma Pinelliae), Tiannanxing (Rhizoma Arisaematis), and Sanqi (Radix Notoginseng). After incubating SPC A1 and drug-bearing rabit serum which at different concentration with serologic pharmacological method, observed and detected the growth and apoptosis of cells, cell cycle and apoptosis rate. Results: By the action of drug bearing rabit serum, SPC A1 grew against the wall and then peeled off gradually; fluorescence microscope showed the nucleus broke into pieces, and cell split into apoptosis bodies in different size; FCM showed a typical peak of apoptosis, which was most obvious after 48 hours' action with 5% drug bearing rabit serum at the rate of 42.82%. Conclusion: One of the antineoplastic molecular mechanism of composed Chinese drug 892 may be its effect on inducing the apoptosis of SPC A1 by serologic pharmacological method.

为探讨中药化痰散结方诱导人肺癌细胞 (SPC A1)凋亡的作用及其对正常人胚肺细胞的毒性作用 ,通过血清药理学方法 ,选择不同浓度的含药兔血清与人肺癌细胞共同孵育处理 ,并以正常人胚肺细胞 (HEL)作对照。结果 :人肺癌细胞经含中药兔血清作用 ,由贴壁生长而逐渐脱落 ,外形变圆 ,体积缩小 ,折光性增强 ;荧光镜下可见细胞核破碎 ,裂解为大小不等的凋亡小体 ;流式细胞仪检测显现典型的细胞凋亡峰 ,以 5%含药血清作用 4 8h凋亡峰最为明显 ,凋亡率为 4 2 .8% ;出现S期细胞阻滞 ;而该含药血清对人胚肺细胞无诱导凋亡作用。化痰散结方通过血清药理学方法诱导人肺癌细胞的凋亡 ,可能是其抗肿瘤的分子学机制之一。

AIM To observe the effect of tea polyphenols (TP) on DNA cleavage and cell cycle of human NPC cell line CNE 2 cells. METHODS To determine cell DNA cleavage with agarose gel electrophoresis technic and to assay CNE 2 cell cycle as well as apoptosis with flow cytometry. RESULTS TP could induce CNE 2 cell DNA cleavage. The degree of DNA cleavage increased with TP concentration increasing and time prolonging of TP treatment. Different TP concentrations and different time of...

AIM To observe the effect of tea polyphenols (TP) on DNA cleavage and cell cycle of human NPC cell line CNE 2 cells. METHODS To determine cell DNA cleavage with agarose gel electrophoresis technic and to assay CNE 2 cell cycle as well as apoptosis with flow cytometry. RESULTS TP could induce CNE 2 cell DNA cleavage. The degree of DNA cleavage increased with TP concentration increasing and time prolonging of TP treatment. Different TP concentrations and different time of TP treatment could disturb different progression of CNE 2 cells at cycle, also could make G 1 phase cell markedly increasing and S phase cells decreasing. Therefore, the cell proliferating index (PI) also decreased. At the same time, TP could induce CNE 2 cell apoptosis. The cell apoptosis rates increase with TP concentration increasing and time prolonging of TP treatment. CONCLUSION TP could induce DNA cleavage of human NPC cell line CNE 2 cells, also could make cells stagnate at G 1 phase and prevent G 1 phase cells to come into S phase. At the same time, TP could induce CNE 2 cell apoptosis. The results will provide theoretical basis for the antitumor action mechanism of TP.

目的 观察茶多酚(tea polyphenols, TP) 对人鼻咽癌细胞株CNE2 细胞DNA 的损伤作用和细胞周期的影响。方法 用琼脂糖凝胶电泳方法测定TP对细胞DNA的断裂作用;用流式细胞术(FCM) 观察TP对CNE2 细胞周期的变化及其诱导凋亡。结果 TP可引起CNE2 细胞DNA 断裂、其断裂程度随剂量的增加和作用时间的延长而增强。TP不同浓度和不同作用时间可干扰CNE2 细胞在周期中的进程,使G1 期细胞明显增多,S期细胞减少,细胞分裂增殖指数(PI)亦随之降低。同时,TP可诱导CNE2 细胞凋亡,其凋亡率随TP浓度的增加和作用时间的延长在逐渐增加。结论 TP19981204 收稿,19990731 修回* 国家自然科学基金资助课题,No 39370800作者简介:谢冰芬,女,副研究员,硕士生导师,研究方向为肿瘤药理可引起人鼻咽癌细胞株CNE2 细胞的DNA 断裂,使细胞停滞于G1 期,阻止G1 期细胞进入S期;同时亦可诱导细胞凋亡,这些结果可能为TP的抗瘤作用提供理论依据

 
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