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凋亡率
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  apoptosis ratio
    Conclusions Smac gene could show high expression in smac gene transferred group cells and increase the apoptosis ratio of EJ cells. Smac gene could further enhance the apoptosis and notably decrease the survival fraction of EJ cells when combined with ~(12)C~(6+) ions irradiation.
    结论外源smac基因转染膀胱癌细胞株能显示高表达,并能提高膀胱癌细胞的凋亡率,与12C6+离子照射结合后能显著降低细胞存活分数和进一步增高凋亡率
短句来源
    Methods:1) Rat mesangial cells were cultrued by fresh serum-free medium 1640 containing 5、10、20、30、40 mM glucose and 30 mM mannitol for 24h、48h、72h and then examined apoptosis ratio by flow cytometry AnnexinV/PI double stains.
    方法:1)大鼠肾小球系膜细胞分别经含5mmol/L、10 mmol/L、20 mmol/L、30 mmol/L、40 mmol/L葡萄糖及含30mmol/L甘露醇的1640培养基培养24h、48h、72h后用流式AnnexinV/PI双染法检测凋亡率
短句来源
    ResuIts: The apoptosis ratio of T24 cells on day 5 with 2.5μmol/L、 5μmol/L and 10μmol/L 4-HPR-treated was 12.17%,28.0% and 26.76%, respectively, and the mortality rate were 3.26%,12.35% and 26.66%,respectively.
    结果2.5μmol/l、5μmol/l、10μmol/l的4-HPR作用于T24细胞5天时的凋亡率分别为12.17%、28.00%和26.76%,死亡率分别为3.26%、12.35%和26.66%。
短句来源
    In the group of treatment with 2.5? 滋mol/L or 5? 滋mol/L 4-HPR and 100cgy γ-radiation, on day 3 the apoptosis ratio were 20.0% and 26.0%, and the average mortality rate of cells were below 5.0%;
    联合作用组细胞分别加入2.5滋m ol/L、5滋m ol/L的4-H PR,100cgy的γ-射线照射一次,3天后T24细胞的凋亡率分别为20.0%、26.0%,细胞的死亡率均在5.0%以下。
短句来源
    whereas on day 5 the apoptosis ratio were 38.0% and 35.0%, and the mortality rate were 12.4% and 21.1% respectively.
    5天后T24细胞的凋亡率分别为38.0%、35.0%,而细胞的死亡率分别为12.4%、21.1%。
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  apoptotic rates
    partial cells presented the morphological changes of apoptosis under the fluorescent microscope,the apoptotic rates of the control group and 4 treatment groups were(9.83±1.53)%,(19.50±1.00)%,(24.83±2.52)%,(30.17±2.08)% and(38.50±2.65)%,respectively;
    荧光显微镜下部分细胞发生凋亡形态学改变,对照组和4个姜黄素不同浓度处理组凋亡率分别为(9.83±1.53)%、(19.50±1.00)%、(24.83±2.52)%、(30.17±2.08)%和(38.50±2.65)%;
短句来源
    The dual staining flow cytometry analysis showed that the early apoptotic rates for 12, 24 and 36 hours were 0.51%, 1.49%, 2.29% for E-J cells respectively, and 59%, 32.78%, 5.19% for BIU-87 cells respectively. The early apoptotic rates of control group for two cell lines were 0.03% and 0.77% respectively.
    MT-12与两株细胞分别作用12h、24h和36h,流式细胞术检测的E-J细胞早期凋亡率分别为0.51%、1.49%和2.29%,BIU-87细胞早期凋亡率分别为59%、32.78%和5.19%,两株细胞的阴性对照组早期凋亡率分别为0.03%和0.77%。
短句来源
    Apoptotic rates of tumor cells attached to FN were 1.4%, 1.0% at 2 hours, 12 hours after exposed to MMC, while those of cells attached to BSA were 4.9%, 8.2% respectively. Apoptotic rates had significant difference between FN and BSA group (P< 0.01).
    FN黏附的膀胱肿瘤细胞在不同浓度MMC作用 2h和 12h后平均凋亡率分别为 1.4 %和 1.0 % ,而BSA组的细胞平均凋亡率为4 .9%和 8.2 % ,两组比较差异有统计学意义 (P <0 .0 1)。
短句来源
    Results The apoptotic rates of sperm in infertility and oligozoospermia groups were significantly higher than that in fertility group (P<0.05).
    结果:不育组及不育组中的少精子症组精子凋亡率均明显高于生育组(P<0·05);
短句来源
    The flow cytometry analysis showed that the apoptotic rates at 3μg/mL concentration for 2, 4, 8, 12, 24 and 36 hours were 46.94%, 51.2%, 28.85%, 20.43%, 6.03% and 5.19% respectively, which were all significantly higher than those in the control group 0.77%.
    3μg/mLMT作用2、4、8、12、24h和36h,双染法流式细胞术检测的细胞凋亡率分别是46.94%、51.2%、28.85%、20.43%、6.03%和5.19%,阴性对照组凋亡率为0.77%。
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  “凋亡率”译为未确定词的双语例句
    ② Flow cytometry techniques showed that the total apoptotic ratio of HCA + AGE group was (15.31±2.14)%, and early apoptotic ratio was (9.16±1.38)%, which were significantly different from those in the HCA group and AGE group (both P < 0.01).
    ②流式细胞仪检测内皮细胞凋亡结果:联合干预组总凋亡率为(15.31±2.14)%,早期凋亡率为(9.16±1.38)%,均显著高于同型半胱氨酸或晚期糖基化终产物单独干预组(P<0.01)。
短句来源
    The percentages of sperm apoptosis were respectively higher than corresponding control group (P< 0.001). The percentages of sperm apoptosis of 0.2 mM (H2O2) group were respectively higher than corresponding 0.02 mM (H2O2) group (P <0.05).
    Percoll优选后,加入H2O2组精子凋亡率显著高于对照组(P<0.001),且0.2mM过氧化氢组精子凋亡率均高于相应的0.02mM过氧化氢组(P<0.05)。
短句来源
    [Conclusion] There would be a positive relation between sperm apoptosis and ROS. The additive H2O2 results in the increased percentage of sperm apoptosis.
    结论精子凋亡率与ROS浓度有正相关关系,外加H2O2增高,精子凋亡率增高。
短句来源
    Clinical significance of the expression of ki-67,bcl-2,bax,p53 and the spontaneous apoptosis in human renal clear cell carcinoma
    人肾透明细胞癌中ki-67 bcl-2 bax p53表达及癌细胞自发凋亡率的临床意义
短句来源
    Significance of the detection of spontaneous apoptosis and the expression of p27kip1 in transitional cell carcinoma of bladder
    联合检测自发凋亡率及p27kip1在膀胱移行细胞癌中的意义
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  apoptosis ratio
EGF at doses of 10 and 100ng/ml could inhibit the cell proliferation significantly, and the apoptosis ratio in high dose of EGF group was higher than in control group.
      
The apoptosis ratio of tumor cells was measured with flow cytometry (FCM).
      
The apoptosis ratio in the group with polyMAG-1000 as gene carrier was (25.11 ± 2.85) %, whereas it was (5.06 ± 1.05) % in the control group with polyMAG-1000 (P>amp;lt;0.01).
      
The apoptosis ratio was as low as (18.31 ± 2.44) % in the group with liposome as gene carrier (P>amp;lt;0.05, as compared with the group with polyMAG-1000 as gene carrier).
      
By calculating the proliferation-apoptosis ratio we found that alloxan did not change the balance between the two mechanisms.
      
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  apoptotic rates
After treatment with 10 μmol/L of Bcl-2 antisense PNA or antisense ODN for 72 h, apoptotic rates of K562 cells were 13.15±1.13 and 11.72±1.12, respectively.
      
Cellular apoptotic rates were determined by using TUNEL.
      
The apoptotic rates and the cell cycles were determined by means of flow cytometry, the apoptosis indexes were detected by using TUNEL, and the expression of caspase-3 was measured by using SP immunohistochemistry.
      
Both models of SBR led to significant increases in enterocyte apoptotic rates compared to respective sham levels; however, apoptotic rates were 2.5-fold higher in ileal compared to jejunal segments (P>amp;lt;0.01).
      
The significant difference in EC apoptotic rates between proximal and distal intestinal segments appeared to be due to utilization of different mechanisms of action.
      
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Theaimofthisstudywastoexaminetheproliferationandapoptosisstatusinnormalprostateandbenignprostatichyperplasia.TheproliferationstatuswasevaluatedbyusingmonoclonalantibodieswhichrecognizetheproliferatingcellnuclearantigenandtheproliferatingassociatednuclearantigenKi67.Theapoptosisstatuswasdetectedbyinsituend-labelingoffragmentedDNAusingtheterminaldeoxynucleotidy1transferasereaction.Atotalof22casesbenignprostatichyperplasiaand20casesofnormalprotatespecimenswerestudied.TheproliferationindexofBPHwasmuchhigherthanthatofnormalprostates,andtheapoptosisindexofhyperplasiaepitheliumwasmuchlowerthanthatofnormalprostaticepithelium.Theresultssuggestthatbothhighproliferatingrateandlowapoptosisrateareassociatedwiththepathogenesisofbenignprostatichyperplasia....

Theaimofthisstudywastoexaminetheproliferationandapoptosisstatusinnormalprostateandbenignprostatichyperplasia.TheproliferationstatuswasevaluatedbyusingmonoclonalantibodieswhichrecognizetheproliferatingcellnuclearantigenandtheproliferatingassociatednuclearantigenKi67.Theapoptosisstatuswasdetectedbyinsituend-labelingoffragmentedDNAusingtheterminaldeoxynucleotidy1transferasereaction.Atotalof22casesbenignprostatichyperplasiaand20casesofnormalprotatespecimenswerestudied.TheproliferationindexofBPHwasmuchhigherthanthatofnormalprostates,andtheapoptosisindexofhyperplasiaepitheliumwasmuchlowerthanthatofnormalprostaticepithelium.Theresultssuggestthatbothhighproliferatingrateandlowapoptosisrateareassociatedwiththepathogenesisofbenignprostatichyperplasia.

为进一步了解良性前列腺增生的病理基础,作者对22例良性前列腺增生和20例正常前列腺的细胞增殖和凋亡情况进行了研究。用免疫组织化学方法检测增殖细胞核抗原和细胞增殖相关抗原来反映细胞的增殖活性,用核酸末端原位标记的方法反映细胞的凋亡情况。结果显示:良性前列腺增生的间质和上皮细胞增殖活性较正常前列腺均明显增高,但上皮细胞的凋亡率却显著下降。作者认为细胞增殖的增加和凋亡的减少两者均参与了良性前列腺增生的发生过程。

To confirm correlation between apoptosis and pathogenesis of renal cell carcinoma (RCC). Methods 14 cases normal renal tissues and 52 RCC was studied by immunohisochemical technique and flow cytometry and cytoimmunofluorescence. Results The rate of apoptosis was 9. 2±1. 61% in normal renal tissue and 4.15±1. 93% in renal cell carcinoma. Apoptosis of renal neoplastic cells occured at lower level ( P <0. 001) while positive rate and relative expression of protein of bcl-2 in RCC were remarkable higher than that...

To confirm correlation between apoptosis and pathogenesis of renal cell carcinoma (RCC). Methods 14 cases normal renal tissues and 52 RCC was studied by immunohisochemical technique and flow cytometry and cytoimmunofluorescence. Results The rate of apoptosis was 9. 2±1. 61% in normal renal tissue and 4.15±1. 93% in renal cell carcinoma. Apoptosis of renal neoplastic cells occured at lower level ( P <0. 001) while positive rate and relative expression of protein of bcl-2 in RCC were remarkable higher than that of normal tissue ( P <0. 05). Apoptotic rate was inversely correlated with expression of bcl-2 ( r = -0. 915 5) P <0. 001), and apoptotic rate of the samples in which bcl-2 was not expression in RCC were lower than that of normal ones. Conclusion The bcl-2 was an inhibtor of apoptosis of RCC, and apoptotic rate in RCC downregulated might help to development and progression of RCC.

目的 为阐明细胞凋亡在肾癌发生、发展中的作用及与bcl-2表达的关系。方法 应用免疫组化结合微波抗原修复和流式细胞荧光技术,对52例肾癌及14例癌旁正常组织的凋亡率及bcl-2的表达进行了定性、定量的测定,并就它们的相关性进行了分析。结果 正常肾组织的凋亡率为9.2±1.61%,肾痛组织的凋亡率为4.15±1.93%。肾癌组织凋亡率低于正常组织(P<0.001)。bcl-2在肾癌组织的阳性率和阳性蛋白细胞含量高于正常肾组织(P<0.05)。凋亡率与bcl-2的表达呈负相关(x=—0.9155,P<0.01),在bcl-2不表达的肾癌中,凋亡率也低于正常组织(P<0.001)。结论 bcl-2有很强的抑制肾癌细胞凋亡的作用,肾癌细胞凋亡率的下降可能有利于肾癌的发生和发展。另外我们推测在肾癌中可能还有其他抑制凋亡的因素存在。

Objective To study the apoptosis(AP) and the androgen and estrogen milieu in normal and hyperplastic prostate tissues. Methods The incidence of apoptosis was detected by means of DNA end labelling method and dihydrotestosterone (DHT) and estrogen (E 2) were radioimmunologically evaluated in 10 normal and 20 hyperplastic prostate tissues. Results No statistical difference was noted of DHT and E 2 density between the transitional and peripheral zones of normal prostate tissue.E 2 appeared to be higher...

Objective To study the apoptosis(AP) and the androgen and estrogen milieu in normal and hyperplastic prostate tissues. Methods The incidence of apoptosis was detected by means of DNA end labelling method and dihydrotestosterone (DHT) and estrogen (E 2) were radioimmunologically evaluated in 10 normal and 20 hyperplastic prostate tissues. Results No statistical difference was noted of DHT and E 2 density between the transitional and peripheral zones of normal prostate tissue.E 2 appeared to be higher in BPH but being statistically insignificant.In normal prostate tissue,the incidence of apoptosis was significantly higher in the peripheral than the transitional zone ( P <0.05) and the incidence of apoptosis(AP) in the transitional zone was significantly higher than that in BPH.No significant correlation was noted between DHT,E 2 density and the insidence of AP either in normal prostate or BPH tissues.The incidence of AP in the transitional zone,where BPH originated,was much less even in the normal prostate tissue and decreased further with aging. Conclusions The reduction of AP density might be the causative factor in the genesis of BPH and the incidense of AP density was not correlated with DHT and E 2 density denoting that the structure and morphology of peripheral and transitional zone of prostate ductal and interstitial tissues are not the same.

目的探讨雄雌激素、细胞凋亡在前列腺增长和前列腺增生(BPH)发病中的作用。方法应用DNA末端原位标记的方法对正常前列腺移行带(10例)和周围带(10例)以及BPH组织(20例)标本进行了细胞凋亡率的研究,并应用放射免疫学技术检测了相应组织的双氢睾酮(DHT)和雌二醇(E2)含量。结果正常前列腺移行带,周围带DHT和E2的差异无显著性,BPH组织中的E2含量(平均6.63ng/g蛋白)虽高于正常前列腺的移行带含量3.15ng/g蛋白,但差异无显著性(P=0.1)。正常前列腺周围带细胞凋亡率(41.2±3.9)%显著高于移行带(29.7±4.0)%,P<0.05,后者又非常显著地高于BPH组织(39±1.1)%,P<0.001。正常与增生前列腺组织内的DHT及E2含量与细胞凋亡率之间无明显相关性。正常前列腺组织中,前列腺增生的起始部位移行带的细胞凋亡率已明显下降,随着年龄的增长,凋亡率进一步下降,导致BPH。细胞凋亡率与其组织的DHT、E2无明显相关性。结论前列腺导管系统的远、近端(周围带、移行带)上皮细胞结构形态不同,其周围的间质亦有差异...

目的探讨雄雌激素、细胞凋亡在前列腺增长和前列腺增生(BPH)发病中的作用。方法应用DNA末端原位标记的方法对正常前列腺移行带(10例)和周围带(10例)以及BPH组织(20例)标本进行了细胞凋亡率的研究,并应用放射免疫学技术检测了相应组织的双氢睾酮(DHT)和雌二醇(E2)含量。结果正常前列腺移行带,周围带DHT和E2的差异无显著性,BPH组织中的E2含量(平均6.63ng/g蛋白)虽高于正常前列腺的移行带含量3.15ng/g蛋白,但差异无显著性(P=0.1)。正常前列腺周围带细胞凋亡率(41.2±3.9)%显著高于移行带(29.7±4.0)%,P<0.05,后者又非常显著地高于BPH组织(39±1.1)%,P<0.001。正常与增生前列腺组织内的DHT及E2含量与细胞凋亡率之间无明显相关性。正常前列腺组织中,前列腺增生的起始部位移行带的细胞凋亡率已明显下降,随着年龄的增长,凋亡率进一步下降,导致BPH。细胞凋亡率与其组织的DHT、E2无明显相关性。结论前列腺导管系统的远、近端(周围带、移行带)上皮细胞结构形态不同,其周围的间质亦有差异,因此即使在接受相同量的雄、雌激素情况下,这些细胞的反应并不相同。

 
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