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tris-hcl缓冲溶液
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  tris-hcl buffer solution
     In the Tris-HCl buffer solution at pH 7.0,REs can react with TAAP to form a stable 1∶3 red complex,which maximum absorption wavelength is located at 523 nm. Beer's law is obeyed in the range of 0.07-1.5 μg/mL.
     在pH7.0的Tris-HCl缓冲溶液中,REs与TAAP生成组成比为1∶3的稳定红色络合物,其最大吸收波长位于523nm处,REs总量在0.07~1.5μg/mL范围内符合比尔定律。
短句来源
     In the Tris-HCl buffer solution of pH7.0,rare earth reacts with 3-thiazodyzo-5-amino pheno1(3-TAP)to form a stable 1∶3 red colour complex having its maximum absorption at 525.3 nm,Beer's law is obeyed in the range of(0.07~1.51)mg·L-1 for RE(Ⅲ).
     在pH7.0的Tris-HCl缓冲溶液中,RE(Ⅲ)与3-噻唑偶氮-5-氨基苯酚形成稳定的1∶3型红色络合物,最大吸收波长为λmax=523nm,RE(Ⅲ)含量在(0.07~1.5)mg.
短句来源
     The fluorescent quenching intensity of protamine sulfate(Ps) at 301nm is linear to the concentration of 8-OHdG in the range of 0.50~7.10μg/ml, at 25℃, in the pH8.2 Tris-HCl buffer solution with the detection limit of 0.18μg/ml.
     在25℃、pH8.2的Tris-HCl缓冲溶液中,在0.50~7.1μg/ml范围内,8-羟基脱氧鸟苷浓度与鱼精蛋白的荧光猝灭强度成线性关系,r=0.9963,方法的检出限为0.18μg/ml,RSD=6.2%,平均加标回收率为104%(n=6)。
短句来源
     In the case of 20mmol/L tris-HCl buffer solution(pH7.0),the formation constant of NdL interacting with native and denatural DNA is 4.11×10~3L/mol.
     在20mm ol/L T ris-HC l缓冲溶液(pH=7.0),N d-四甘醇醛缩苯丙氨酸Sch iff碱配合物与小牛胸腺DNA的结合常数为4.11×103L/m ol,化合物本身的UV和FL峰强度减小。
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  “tris-hcl缓冲溶液”译为未确定词的双语例句
     The experimental results showed a linear range of Hg~(2+) concentrations from 1.0×10~(-5) to 1.0×10~(-4) mol·L~(-1) in a Tris-HCl buffer at pH 8.0 with fast response and good repetition.
     结果表明,在pH 8.0的Tris-HCl缓冲溶液中,Hg2+的浓度与传感膜的荧光猝灭比值在1.0×10-5~1.0×10-4mol.
短句来源
     was loaded on Sepharose CL-2B column (inner diameter 20 mm, gel column height 46 cm) which had been previously equilibrated with pH 7.5 Tris-buffer containing Mg2 + O. OOlM and KCl 0.1M. The column was developed with the same buffer.
     Sepharose CL-2B柱(内径20mm)床高46cm,核糖体亚基上柱量225mg(湿重),平衡液及冼脱液均为含Mg~(2+)0.001M和KCl 0.1M的Tris-HCl缓冲溶液(pH7.5),流速12ml/h,部分收集,测各流分的OD_(258)。
短句来源
     The mechanism of interaction between Sm(Ⅲ)(MTB)2 [methylthymol blue-Sm(III)] metal com- plex and herring sperm DNA has been studied by using neutral red (NR) as a spectral probe with the ways of UV-Vis spectra, fluorescence spectra and viscometric method in buffer solution of Tris-HCl (pH=7.25).
     在pH=7.25的Tris-HCl缓冲溶液中,以中性红(NR)作为光谱探针,采用UV光谱、FS光谱、粘度等方法研究了甲基百里酚蓝(MTB)与稀土金属离子钐[Sm(Ⅲ)]形成的配合物Sm(Ⅲ)(MTB)2与鲱鱼精DNA的作用机制.
短句来源
     In pH 7.20 Tris-HCl buffer,AOⅡ has a sensitive reduction peak at-0.69 V(vs.SCE). The addition of BSA to the above solution,the reduction peak current of AOⅡ decreases and reduction peak potential shifts positively. An electrochemical quantitive method of BSA was developed based on the interaction of AOⅡ with BSA.
     在pH 7.20的Tris-HCl缓冲溶液中,AOⅡ有一灵敏的还原峰,峰电位Ep约为-0.69 V(vs.SCE),加入BSA后AOⅡ还原峰电位正移,峰电流下降,据此建立了一种测定BSA的电分析方法。
短句来源
     In Tris-HCl buffer suolution(pH=4.0),the crystal violet reacts with protein to form stable red complexes with λ_(max) of 575 nm. The apparent molar absorbance of the complex was 3.25×10~5 L/(mol ·cm). Beer′s law was obeyed in the range of 0.0-(80.0) μg/mL for protein content.
     在pH=4.0的Tris-HC l缓冲溶液中,结晶紫与蛋白质结合形成稳定的深红色配合物,该配合物的最大吸收波长为575 nm,表观摩尔吸光系数为3.25×105L/(mol.cm),蛋白质含量在0.0~80.0μg/mL范围内服从朗伯比耳定律,检出限为6.47μg/mL,回收率为97%~101%。
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  相似匹配句对
     Determination can be made at pH 7.0,where the native structure of DNA is not disrupted.
     L-1,Tris-HCl缓冲溶液,pH 7.0。
短句来源
     HCL solution.
     HCL溶液平衡。
短句来源
     The sintered ceramics were soaked in Tris solution.
     将复相陶瓷浸泡在Tris缓冲溶液中降解。
短句来源
     solution;
     溶液;
短句来源
     THEORETICAL EXAMINATIONS ON LIGAND BUFFER SOLUTIONS
     配位体缓冲溶液的理论考察
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  tris-hcl buffer solution
The strongest activating effect was observed in a Tris-HCl buffer solution at pH 9.8.
      
Plasmin activity was determined with a semiquantitative method using dry punches of filter paper previously soaked in 0.1 M Tris-HCl buffer solution containing mmol/l d-Val-Leu-Lys-FCA (trifluoromethylaminocoumarine), pH 7.2.
      
High extraction (97%) of the cutinase was achieved at pH 7.0 with a 50 mM Tris-HCl buffer solution containing 100 mM KCl, but a low activity was detected in the reversed micellar phase.
      
In Tris-HCl buffer solution, nucleic acids combine with cysteine-capped nano-ZnS particles by intermolecular forces to form larger nanoparticles.
      
The influence of different buffers and concentration of the nanoparticles on the relative fluorescence intensity was tested, and Tris-HCl buffer solution proved to be the best.
      
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With a favorable result, we applied Sepharose CL-2B column to the isolation of ribosomal subunit, 50S, from P. Vulgaris cells. This report presents a simple and useful method for preparative separation of subunit species.Ribosomal preparation 225mg (wet wt.) was loaded on Sepharose CL-2B column (inner diameter 20 mm, gel column height 46 cm) which had been previously equilibrated with pH 7.5 Tris-buffer containing Mg2 + O.OOlM and KCl 0.1M. The column was developed with the same buffer. Elution was carried out...

With a favorable result, we applied Sepharose CL-2B column to the isolation of ribosomal subunit, 50S, from P. Vulgaris cells. This report presents a simple and useful method for preparative separation of subunit species.Ribosomal preparation 225mg (wet wt.) was loaded on Sepharose CL-2B column (inner diameter 20 mm, gel column height 46 cm) which had been previously equilibrated with pH 7.5 Tris-buffer containing Mg2 + O.OOlM and KCl 0.1M. The column was developed with the same buffer. Elution was carried out with a flow rate of 12 ml/h. The adsorbance of the eluate was measured at 258hm. Two peaks were obtained in elution development. The result was checked up to be identical with that observed in ultracentrifuge analysis.According to the proteins contained, the percent recovery of subunit species was 91% in approximate estimation.

我们应用Sepharose CL-2B柱分离变形杆菌核糖体亚基50S获得满意结果,为该亚基制备性分离提供了一简便而适用的新方法。 Sepharose CL-2B柱(内径20mm)床高46cm,核糖体亚基上柱量225mg(湿重),平衡液及冼脱液均为含Mg~(2+)0.001M和KCl 0.1M的Tris-HCl缓冲溶液(pH7.5),流速12ml/h,部分收集,测各流分的OD_(258)。洗脱曲线呈现两个峰,其结果与样品超速离心分析结果一致,按蛋白质含量粗略计算,核糖体亚基回收率为91%。

An enzymic electrode and a tissue electrode were made by mounting uricaseand big mouse fresh liver onto the different oxygen e1ectrodes. The testing conditions,analytic properties and app1ications were measured and studied. The optilnal conditionsof the tissue electrode and that of enzymic electrode were respectively determined bel-low:33℃, pH= 9(Tris-HCl buffer); linear range 3.3-56.67μg/mL, 28℃, pH = 8. 8(Na2B4O7-(NH4)2) SO4 buffer), linear range 6.67-133.3μg/mL. The CV of detectingvalues of the two electrodes...

An enzymic electrode and a tissue electrode were made by mounting uricaseand big mouse fresh liver onto the different oxygen e1ectrodes. The testing conditions,analytic properties and app1ications were measured and studied. The optilnal conditionsof the tissue electrode and that of enzymic electrode were respectively determined bel-low:33℃, pH= 9(Tris-HCl buffer); linear range 3.3-56.67μg/mL, 28℃, pH = 8. 8(Na2B4O7-(NH4)2) SO4 buffer), linear range 6.67-133.3μg/mL. The CV of detectingvalues of the two electrodes all are less than 4% and the Michaelis constants of the en-zymic e1ectrode and tissue electrode were 2. 8×10~(-4) mol/L and 3. 3 × 10~(-4) mol/L respec-tively. The data of the measurments in detecting control serum with the two electrodeswere all in allowing range. With further study and development the two electrodes canbe used in clinic.

将大白鼠肝脏切片和尿酸酶分别固定在不同氧电极上,制成尿酸组织电极和酶电极,研究了它们的测试条件、分析性能和应用情况.结果:组织电极的最佳测试条件为33℃,pH=9,Tris-HCl缓冲溶液,线性范围3.33~56.67μg/mL,K_M=3.3×10~(-4)mol/L;酶电极的最佳测试条件为28℃,pH=8.8,Na_2B_4O_7-(NH_4)_2SO_4缓冲溶液,线性范围6.67~133.3μg/mL,K_M=2.8×10~(-4)mol/L.二种电极测量值的变异系数均小于4%,电极性能各有优缺点.用它们测定质量控制血清,测量值均在示值范围内,有望应用于临床.

The PVC membrane lithium ion-selective electrodes based on lipophilic neutral carrier ETH1810 were prepared. The membrane composition was optimized by variation of the content of anion site KTpClPB. A membrane containing 1. 0% ETH1810, 0. 2% KTpClPB,33. 0%PVC and 66. 0% 2-NPOE was obtained. The slope of the electrode was 56. 0 mV/decade, with the linear range of 3. 2×10-5-1. 0×10-1mol/L and the detection limit of 1. 6×10-5mol/L. The electrode showed moderate selectivity for Li+ over Na+ and K+. The other characteristics...

The PVC membrane lithium ion-selective electrodes based on lipophilic neutral carrier ETH1810 were prepared. The membrane composition was optimized by variation of the content of anion site KTpClPB. A membrane containing 1. 0% ETH1810, 0. 2% KTpClPB,33. 0%PVC and 66. 0% 2-NPOE was obtained. The slope of the electrode was 56. 0 mV/decade, with the linear range of 3. 2×10-5-1. 0×10-1mol/L and the detection limit of 1. 6×10-5mol/L. The electrode showed moderate selectivity for Li+ over Na+ and K+. The other characteristics of the electrode were also satisfactory. It is possible to use the electrode to assay Li+ in blood within the clinically relevant concentration range or in other real samples why flow injection analysis.

本文报道一种基于中性载体的PVC膜锂离子选择电极,采用两种增塑剂和不同量的阴离子定域体对电极膜进行优化,考察了影响电极响应和电极稳定性的一些主要因素。其中以含1.0%ETH1810、0.2%KTpClPB、66.0%2—NPOE和33.0%PVC的膜组成制备的电极在pH8.0的0.05mol/L Tris-HCl缓冲溶液中对锂离子呈良好的Nernst响应,斜率为56.0mV/decade,线性响应范围为3.2×10~(-5)—1.0×10~(-1)mol/L,检测下限为1.6x10~(-5)mol/L,电极具有很好的稳定性和重现性,对Na~+和K~+的选择性系数K(?)分别为:5.0×10~(-3)、3.2×10~(-3)。加标回收方法测试血清样品,说明了临床分析应用的可能性。

 
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