. These showed supplementation of 7mmol/L taurine in the medium could improve the morulae rate and blastocyst rate significantly (P＜0.05),and adding to14mmol/L taurine at 4-8 cell period got the best effect.
The results showed that Yikang"xp" could improve the litter size and alive litter size of sows,the alive litter size was improved by 10.90% compared with the control group,the difference was statistically significant(P<0.05).
The results showed that:Compared with the groups without GMOS,dietary supplementation with GMOS could significantly increase the levels of IL-6,CORT and GH in milk(P<0.05),elevate milk yields(P=0.07)and the content of lactoprotein(P=0.06)in milk.
the treated Ⅰ and Ⅱ and Ⅲ groups were superior to the control one for average daily feed intake(P<0.01), the content of average daily feed intake increased by 16.84%,37.28%,41.22%,but average daily feed intake increased with increase of diet phytase in levels.
fumigatus Q23L phytase resulted in a re markable increase in specific activity around pH4.5～7.0,but the pH stability of Q23L was lower than A. fumigatus wild-type phytase. To increase the pH stab ility of Q23L,the mutant Q23LG272E was constructed by site-directed mutagenesis wit h PCR.