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医疗
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Sheep red blood cells(SRBC), treated with tannic acid and glutaralde-hyde were sensitized with cysticercus fluid antigen, lyophilized and stored in ampoules at 4℃. Using such lyophilized sensitized SRBC a passive he-magglutination reaction on polystyrene plates was developed for the diagnosis of cysticercosis. Serum samples from 113 cases of cysticercosis and 196 cases of other diseases and normal controls were examined. A comparison was made of this reaction in parallel with an ordinary passive hemaggluti-nation...

Sheep red blood cells(SRBC), treated with tannic acid and glutaralde-hyde were sensitized with cysticercus fluid antigen, lyophilized and stored in ampoules at 4℃. Using such lyophilized sensitized SRBC a passive he-magglutination reaction on polystyrene plates was developed for the diagnosis of cysticercosis. Serum samples from 113 cases of cysticercosis and 196 cases of other diseases and normal controls were examined. A comparison was made of this reaction in parallel with an ordinary passive hemaggluti-nation reaction and a complement fixation test. It was found that the he-magglutination reaction posseses the following advantages:1) High sensitivity with a postive rate of 95.6% in. proved cases of cysticercosis. Comparison of the three tests among 100 cases showed a higher positive rate by the two hemagglutination tests(98%)than the complement fixation test(91%), the hemagglutination test using lyophilized sensitized SRBC usually giving a higher titer than the ordinary hemagglutination reaction. 2) High specificity. There was no cross reaction in 25 cases of clo-norchiasis, 1 case of echinococcosis, 19 cases of brain tumor and 1 case of tuberculous pleurisy and there was no false positive reaction in 150 healthy subjects.3 ) Only 25 μ1 of serum are needed for each test , and so blood from earlobe and finger tips can be used.4) Performance is simple and endpoint can be read within 1 hour.5) The lyophilized sensitized SRBC can be kept for more than one year without loss of activity.

本文报导用冻干致敏羊红细胞进行囊虫病间接血凝平板微量试验检查囊虫病113例及其他疾病和健康对照共196例的结果,同时与一般间接血凝和补体结合试验进行了平行对比观察,发现本法具有下述主要优点;(1)灵敏度高,检出率高达95.6%。在100例囊虫病的三种方法平行对比观察中,两种间接血凝法检出率为98%,超过补体结合试验(91%),而冻干间接血凝效价高于一般间接血凝试验。(2)特异性较强,肝吸虫病25例,棘球蚴病1例,脑瘤19例,胸膜结核1例,健康人150例均未出现阳性反应。(3)血清用量少,每次试验只需样品25微升(可用耳血或指血)。(4)操作简单,反应快速,一小时内即可报告结果。(5)保存时间长,抗原致敏的羊红细胞冻干后保存,一年后效价仍稳定不减。鉴于以上优点,我们建议由生物制品部门批量生产供应基层医疗防治单位应用。

1,146 swab rinse samples of 6 medical institutions in Shanghai were collected from their laboratories and stomatological clinics. The occult blood was traced by benzidine method and hepatitis B surface antigen (HBsAg) was detected by enzyme linked immunosorbent assay (ELISA). Prevalence of serum HBsAg among out-patients was 9.8-14.3% while in the blood smears was about 5.5%. HBsAg was also detected from the surfaces of tables, basins, needles, hands and some medical apparatus. These findings showed that a variety...

1,146 swab rinse samples of 6 medical institutions in Shanghai were collected from their laboratories and stomatological clinics. The occult blood was traced by benzidine method and hepatitis B surface antigen (HBsAg) was detected by enzyme linked immunosorbent assay (ELISA). Prevalence of serum HBsAg among out-patients was 9.8-14.3% while in the blood smears was about 5.5%. HBsAg was also detected from the surfaces of tables, basins, needles, hands and some medical apparatus. These findings showed that a variety of objects in hospitals had been contaminated by blood. The presence of HBsAg on various surfaces was directly correlated with the contamination of occult blood. The rate of detection of HBsAg was 3-9%, higher than those without blood contamination (0.9-1.6%). In addition, laboratory experiments verified that HBsAg was relatively stable even after 6 weeks under various environmental conditions. The speed of decline in titers of HBsAg under 25℃ was faster than under 4℃. This contamination might serve as an important role of transmission, therefore, strict disinfection should be practised as a routine in the hospitals so as to further control the spread of viral hepatitis B.

本文介绍了上海市6个医疗机构中的化验室、口腔科进行HBsAg污染情况调查和稳定性研究结果。在1,146份采样中,门诊病人血检标本的HBsAg阳性率为9.8~14.3%,血玻片的阳性率5.5%,尤其在工作台、水斗表面、口腔科器械、工作人员的手以及“消毒”器械均查到HBsAg。以上情况已成为乙型肝炎传播的一条重要途径,因此,医疗机构中严格执行消毒制度,对控制乙型肝炎的发病有一定的流行病学意义。

Dot-immunobinding assay is a new serodiagnostic method. The antigen-antibody immunoreaction is carried out on nitrocellulose membranes as the solid supporter and the immunoreaction is detected by the second antibody conjugated with enzyme and substrate. The study of sensitivity and specificity in the diagnosis of hydatid disease by dot-immunobinding assay with 52 sera from clinically diagnosed cystic hydatid disease patients, 31 sera from other patients and 60 sera from healthy blood donors was carried out....

Dot-immunobinding assay is a new serodiagnostic method. The antigen-antibody immunoreaction is carried out on nitrocellulose membranes as the solid supporter and the immunoreaction is detected by the second antibody conjugated with enzyme and substrate. The study of sensitivity and specificity in the diagnosis of hydatid disease by dot-immunobinding assay with 52 sera from clinically diagnosed cystic hydatid disease patients, 31 sera from other patients and 60 sera from healthy blood donors was carried out. When the concentration of the sera was 1:400 the positive rates were 0 and 3.2% with the normal sera and the sera from other patients respectively, but the positive rate was 92.3% with sera from hydatid disease patients. 1 serum from the patient with non-parasitic lung cyst reacted with the crude antigen from sheep hydatid cyst fluid weakly. 1 serum from the patient with cysticercosis crossreacted with the crude antigen from sheep hydatid cyst fluid completely. The results indicate that dot-immunobindingassay is sensitive and specific for detecting the antibody level of the patients with hydatid disease. The cost is low to diagnose hydatid disease by this method and also there is no need of the special complex instruments and experimental condition. The method is suitable to popularize for basic medical units.

作者首次应用点免疫结合法诊断棘球蚴病。52例棘球蚴病患者、31例其它疾病患者、60例正常人和1例猪囊虫病患者的诊断试验结果证明,此法诊断棘球蚴病敏感性高,特异性强,方法简便,适于在农牧区基层医疗单位推广。

 
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