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scar检测
相关语句
  scar detection
     THE DEVELOPMENT OF SCAR DETECTION MARKER OF PUCCINIA STRIIFORMIS F. SP. TRITICI RACE CY31 IN CHINA
     小麦条锈菌条中31号生理小种SCAR检测标记的建立
短句来源
     Using a simple protocol, intact genomic DNA can be obtained for SCAR detection. This method does not use organic solvents and centrifuge equipment.
     用一种简单的方法 ,无需接触有毒的有机试剂 ,无需离心 ,即可从小麦叶片中提取完整的基因组DNA ,质量满足SCAR检测的要求。
短句来源
  “scar检测”译为未确定词的双语例句
     A Simple Genomic DNA Extraction Process for SCAR Analyses
     用于SCAR检测的基因组DNA简易提取法
短句来源
     Selection and foundation of a race specific molecule marker for wheat stripe rust race CY29 in China
     小麦条锈菌条中29号生理小种SCAR检测标记的建立
短句来源
     Developing SCAR Markers and FQ-PCR to Study Predation on Bemisia Tabaci
     天敌对烟粉虱捕食作用的SCAR检测和FQ-PCR评价
短句来源
     2. STS and SCAR analysis carried out in FI and F2 of ThLr35 showed that the 900 bp bind appeared in FI and F2 plant conferring resistance against leaf rust as well as in ThLr35, but not in the susceptible of F2. It proved the STS and SCAR markers are reliable for Lr35 detection.
     2.对ThLr35的F_1代植株进行PCR-STS和PCR-SCAR检测,扩增结果表明F_1代中有900bp的DNA片段,说明ThLr35的F_1代含有Lr35基因。 F_2代中表现感病的植株没有900bp的DNA片段,表现抗病的植株有900bp的DNA片段。
短句来源
     Detecting F_3 individuals from the cross-resistant with susceptible cultivator BTAM428×ICS-12B and other related cultivars,results showed that two SCAR marker(OPN-07_(727),OPN-08_(373))were existed.
     用与抗蚜基因紧密连锁的OPN - 0 772 7、OPN - 0 8373RAPD标记 ,转化得到的SCAR标记对F3代及部分抗感品种进行SCAR检测
短句来源
  相似匹配句对
     A Simple Genomic DNA Extraction Process for SCAR Analyses
     用于SCAR检测的基因组DNA简易提取法
短句来源
     spectroscopy detection.
     能谱检测
短句来源
     Detection of Predation on Bemisia tabaci by Using SCAR Markers
     天敌对烟粉虱捕食作用的SCAR标记检测
短句来源
     Organelles of E.
     检测感染 E.
短句来源
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  scar detection
Bands 1 and 2 working in red and near infrared respectively was utilised, which made burn scar detection simpler with a resolution of 250 metres.
      
It is divided into four components corresponding to preparation, hotspot detection, burn scar detection and final confirmation.
      
To analyse these burning practices MODIS proved to be a very suitable option for burn scar detection.
      
This is important for burn scar detection in cloudy regions as it provides many alternative days for analysis.
      
These organisations often use a combination of active fire detection and burn scar detection.
      


Five primers, with which polymorphism bands could be amplified between tw o DNA pools of the parents of hybrid rapeseed, Shuza No.6 , were screened out fro m 100 RAPD primers. Then these five primers were used to amplify the total DNAs of F 1 to determine their specificity and stability. Several maternal specific an d paternal specific DNA bands were amplified with GE204 and GE222, in the DNA pools of Shuza No.6 and its parents. respectively In order to convert the RA PD markers into more stable...

Five primers, with which polymorphism bands could be amplified between tw o DNA pools of the parents of hybrid rapeseed, Shuza No.6 , were screened out fro m 100 RAPD primers. Then these five primers were used to amplify the total DNAs of F 1 to determine their specificity and stability. Several maternal specific an d paternal specific DNA bands were amplified with GE204 and GE222, in the DNA pools of Shuza No.6 and its parents. respectively In order to convert the RA PD markers into more stable SCAR (Sequence characterized amplified region) markers, the maternal specific marker GE204 500 and paternal specific marker GE22 2 300 w ere cloned and sequenced respectively. When SCAR primers designed according to t he sequences of GE204 500 and GE222 300 were mixed and used to amplify DNAs of Shu za No.6 and its parents, two distinctive bands, one of which was maternal spec ific and the other was paternal specific, appeared in all hybrid plants, but on ly one parent specific band in maternal or paternal plants. The reliability of this result was confirmed by the phenotypes of hybrid seed lots in the f ield.

用 10 0个随机引物对“蜀杂 6号”父、母本进行 RAPD扩增 ,选出 5个能在父、母本中产生多态性扩增的引物。在杂种 F1代中验证它们的特异性和稳定性 ,从中筛选出能从杂种 F1代中稳定扩增“蜀杂 6号”父、母本特异标记的随机引物 GE2 0 4和 GE2 2 2 ,并对它们扩增的父、母本特异标记片段进行克隆和测序。根据测序结果设计的特异序列扩增引物 ,将“蜀杂 6号”的父、母本 RAPD标记转化为序列特异的 SCAR标记。通过对“蜀杂 6号”父、母本和 30 0个杂种 F1代单株的父、母本特异序列扩增标记 (SCAR)的检测 ,建立了用 SCAR- PCR检测杂交油菜“蜀杂 6号”杂种纯度的方法 ,并逐一对照大田杂种一代生产种的表现型 ,验证了本方法的可靠性

Using a simple protocol, intact genomic DNA can be obtained for SCAR detection. This method does not use organic solvents and centrifuge equipment. The process can prepare mass samples in a short time and fit for molecular marker detection of F 2 segregant population and screening of breeding materials.

用一种简单的方法 ,无需接触有毒的有机试剂 ,无需离心 ,即可从小麦叶片中提取完整的基因组DNA ,质量满足SCAR检测的要求。该方法可在短时间内制备大量样品 ,适于进行遗传连锁性分析时对F2 分离群体的单株检测和分子标记辅助选择时筛选育种材料 ,操作简单 ,实用性强 ,值得推广。

Rapid identification of the races of Puccinia striiformis f. sp. tritici by molecular technique was very important to monitor and control of wheat stripe rust in China. In this paper, a molecular identification method for a Chinese race of Puccinia striiformis f. sp. tritici CY31 was developed by means of SCAR-PCR technique. Five Chinese races of P. striiformis f. sp. tritici were analysed with 210 primers by RAPD technique. A special DNA fragment of race CY31 was founded and cloned. Based on the sequencing...

Rapid identification of the races of Puccinia striiformis f. sp. tritici by molecular technique was very important to monitor and control of wheat stripe rust in China. In this paper, a molecular identification method for a Chinese race of Puccinia striiformis f. sp. tritici CY31 was developed by means of SCAR-PCR technique. Five Chinese races of P. striiformis f. sp. tritici were analysed with 210 primers by RAPD technique. A special DNA fragment of race CY31 was founded and cloned. Based on the sequencing result, specific PCR primers were designed and a SCAR marker for race CY31 was obtained successfully. The results here indicates that the identification of the races of Puccinia striiformis f. sp. tritici could be improved greatly through cosmically searching special RAPD fragments of different races.

建立小麦条锈菌Pucciniastriiformisf.sp.tritici生理小种的快速分子检测技术对我国小麦条锈病的监测和防治策略的制定具有重要价值,本文首次报道了利用SCAR—PCR技术进行条锈菌生理小种分子检测的方法。通过对我国目前主要优势小种条中31号RAPD片段的规模筛选,在对特异片段回收、克隆、测序的基础上,设计特异PCR引物,成功获得了条中31号生理小种专化的SCAR检测标记。

 
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