Karyotypes and Giemsa N-banding patterns of 9 hexaploid species,8 tetraploid species and 2 diploid species, and the probable donor of B genome, Aegilops sharonensis,have been studied by means of new method of preparing mitotic chromosome specimen in plant and N-banding technique.
The banding patterns of B genome of hexaploid wheats are basically same, but N-bands have definite differences among species of B genome of tetraploid wheats.
Definite differences in the degree of heterochromati(?) ixation and the distribution of heterochromatin were discovered between A genome chromosomes of tetraploid wheats with AABB or AAGG genomic constitution and the T. (?)
searsii等进行核型分析和染色体N—分带,结果显示出在一粒小麦中不存在与4 A有相似N—带型的染色体,四倍体小麦A A B B和A A G G中的A组染色体与一粒小麦的染色体在异染色体质化程度和异染色质分布方面均有一定程度差异,表明A组染色体在进化过程中亦已发生了分化;
hybrids were studied. Results further demonstrated that the B and G genome chromosomes (exc(?) usive of individual exceptions) and chromosome 4A showed rather high homology with the chromosomes of Ae.
Diploid species show no Nbands and have no chromosome correspondent to 4A according to banding patterns. Therefore, further study on the origin of 4A chromosome is needed.
Seven complete chromosomes and nine telocentric chromosomes in telotrisomics of barley (Hordeum vulgare L.) were identified and designated by an improved Giemsa N-banding technique.
DNA sequence organization correlates with the distribution of major Giemsa C-band regions as well as with N-bands and the binding sites of fluorescent dyes.
From the available cytological and biochemical data it was suggested that the N-bands represent certain structural non-histone proteins specifically linked to nucleolar organizers in various eukaryotic chromosomes.
Since N-bands are not evident on some nucleolar organiser chromosomes, the staining specificity cannot be correlated with the presence of nucleolar organiser regions.
The present paper is dealing with the investigation of the chromosome N-banding technique and the N-banding patterns in Hordeum vulgare,Triticum aestivum,Secale cereale,Vicia faba and Allium cepa.Two N-banding techniques were applied.First, the chromosome slides were stained with Giemsa solution.Second,the slides were treated in 1M NaH_2PO_4 solution at 92-94℃ for 3.5-8.5 min.After rinsing in tap water they were stained with Giemsa solution.The experiments have demonstrated that the N-banding technique is s...
本文研究了大麦、小麦、黑麦、蚕豆、洋葱等染色体的 N 带带型鉴定技术。采用了两种 N 带技术:第一,将染色体标本在5%三氯醋酸(90℃,6分钟)-0.1NHCI(60℃,6分钟)处理,Giemsa染色;第二,染色体标本经 IM NaH_2PO_4(92—94℃,3.5—8.5分钟)处理,Giemsa 染色。试验证明 N 带技术简单、快速,带型清晰。带型分析表明,N 带并非专一地显示核仁组织者。将上述植物的 N 带与 C 带比较,在有些植物中虽然有些 N 带区与 C 带区一致,但也有些区域仅显示出 N 带而无 C 带或仅显示 C 带而无 N 带。为此,N 带技术与 C 带技术的结合使用,将对染色体鉴别十分有价值。N 带同 C 带一样也是细胞遗传学中一个有用的标记。
Karyotype of two-rowed wild barley (Hordeum spontaneum C. Koch) of Qing-Zang (Tibet) Plateau was essentially the same as that of the Middle East, and chromosome Gie-msa N-banding patterns of the former were similar to that of the latter. The results showed that H. spontaneum of Qing-Zang is closely related to that of the Middle East. Although there is slight difference between them which is only an intraspecific one. Meantime, it was found that there were a few significant interspecific differences between ...
The present paper deals with the investigation of chromosome N-banding pattern in rice. It was found that all the 12 pairs chromosomes of cultivated rice possessed prominent banding, which shows a form of point.N-bands were mainly distributed near centromere in rice. In addition, interstitial bands, near terminal bands and terminal bands were also observed. Our results obtained showed that the form and position of N-bands of rice chromosome was different from common wheat, barley, Aegilops speltoids, maize ...
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