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基因工程hbsag
相关语句
  recombinant hbsag
     Pilot Study on Purification Process of Recombinant HBsAg by Column Chromatography
     柱层析法纯化基因工程HBsAg中试工艺的研究
短句来源
     Recombinant HBsAg was absorbed to Al(OH)3. The results of immu-nogenicity in Balb/c mice showed that ED50 of cellular HBsAg was 0.501 μg, but that of human plasma HBsAg was 0.832μg. The immunogenicity of cellular HBsAg was some what better than that of human plasma HBsAg.
     将纯化的HBsAg吸附于氢氧化铝佐剂,免疫Balb/c小鼠,并与血源HBsAg对照,抗体半数阳转剂量(ED50)分别为0.501μg和0.832μg,说明基因工程HBsAg的免疫原性似优于血源HBsAg。
短句来源
  “基因工程hbsag”译为未确定词的双语例句
     HBsAg purified from cell culture supernatant of B43 consisted of homogeneous 22-nm particles under the electron microscope. The amounts of residual calf serum and residual cellular DNA were less than 30ng/dose and 31.25pg/dose respectively. The quality of pure HBsAg is over the standards set by WHO.
     由此流程HBsAg最终回收率为50%~65%,所获纯品纯度达95%以上,经电镜检查,牛血清残余量(<8.430ng/Dose)和细胞DNA残余量(<32pg/Dose),均符合WHO规定的基因工程HBsAg人体接种的标准。
短句来源
     The immunogenicity test in mice showed that cellular HBsAg was 1.55-2.64 times more potent than that blood-derived HBsAg. HBsAg treated with 0.025% formalin produced higher HBsAb than that without formalin.
     动物实验证明:用与血源HBsAg疫苗同等剂量的基因工程HBsAg疫苗接种Balb/C小鼠,可获得比血源疫苗高2.64倍的免疫效果。
短句来源
  相似匹配句对
     Producing HBsAg protein by the way of genetic engineering
     用基因工程手段生产HBsAg蛋白
短句来源
     Genetically engineered antibodies
     基因工程抗体
短句来源
     gene engineered vaccine
     基因工程疫苗
短句来源
     HbsAg, HbsAb;
     HbsAg、HbsAb;
短句来源
     HBsAg was positive;
     HBsAg阳性;
短句来源
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  recombinant hbsag
Therefore, as well as in recombinant HBsAg-yeast cells, assembling of HBsAg monomers into immunogenic aggregates takes place in HBsAg-transgenic potato, which can be used as a source of recombinant vaccine against hepatitis B virus.
      
Immunochemical Study of Antigenic Determinants of Recombinant HBsAg Produced by Bombyx mori Larvae
      
Antibodies to various protein fractions isolated from larvae of mulberry silkworm containing recombinant HBsAg, to plasma HBV, and to healthy human serum were obtained.
      
The antigenic determinants of recombinant HBsAg are identical to those in plasma and have practically no common antigenic determinants with healthy human blood serum
      
Preparation and Properties of Monoclonal Antibodies to Recombinant HBsAg Produced by Silkworm Larvae
      
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The purification of HBsAg in MT-5 cells culture supernatant was carried out by three different steps including ultrafiltration, precipitation by polyethylene glycol ( PEG ) and three-step ultracentrifugations. The purified HBsAg was composed of two bands in silver stained SDS-PAGE, their molecular weights being 23k and 27k respectively which were identical to those of human plasma HBsAg. There was no other protein band in the gel. The purity of purified HBsAg accorded with the requirement of hepatitis B vaccine....

The purification of HBsAg in MT-5 cells culture supernatant was carried out by three different steps including ultrafiltration, precipitation by polyethylene glycol ( PEG ) and three-step ultracentrifugations. The purified HBsAg was composed of two bands in silver stained SDS-PAGE, their molecular weights being 23k and 27k respectively which were identical to those of human plasma HBsAg. There was no other protein band in the gel. The purity of purified HBsAg accorded with the requirement of hepatitis B vaccine. Up to 44.1% of starting HBsAg was recovered at the end of the purification process.Recombinant HBsAg was absorbed to Al(OH)3. The results of immu-nogenicity in Balb/c mice showed that ED50 of cellular HBsAg was 0.501 μg, but that of human plasma HBsAg was 0.832μg. The immunogenicity of cellular HBsAg was some what better than that of human plasma HBsAg.

用乙型肝炎病毒(HBV)DNA转染工程细胞株MT-5,收获培养上清液,经超过滤浓缩、PEG沉淀和3次超速离心,得到纯化的HBsAg。经SDS-PAGE银染色后,纯化的HBsAg显示两条多肽,分子量分别为23k和27k,与血源HBsAg的多肽成分相同,为HBsAg的两条特异性多肽。经PAGE银染色结果显示,纯化的HBsAg中杂蛋白含量符合疫苗制备要求。用上述方法提纯HBsAg,回收率可达44.1%以上。 将纯化的HBsAg吸附于氢氧化铝佐剂,免疫Balb/c小鼠,并与血源HBsAg对照,抗体半数阳转剂量(ED50)分别为0.501μg和0.832μg,说明基因工程HBsAg的免疫原性似优于血源HBsAg。

Pooled supernatants containing HBsAg secreted by CHO-B43 cell line from rolling bottle culture were clarified and solid ( NH4 ) 2S04 was added to a final concentration of 0.5 saturation. The precipitate was collected by centrif ugation ( 30min at 7500r/min, room temperature ) and washed once with 45% ( NH4 ) 2SO4. The pellets were dissolved in a minimum volume of normal saline, dialysed and concentrated by ultrafiltration. KBr was added to a density of 1.24 and 1.20g/cm3 respectively and two successive runs...

Pooled supernatants containing HBsAg secreted by CHO-B43 cell line from rolling bottle culture were clarified and solid ( NH4 ) 2S04 was added to a final concentration of 0.5 saturation. The precipitate was collected by centrif ugation ( 30min at 7500r/min, room temperature ) and washed once with 45% ( NH4 ) 2SO4. The pellets were dissolved in a minimum volume of normal saline, dialysed and concentrated by ultrafiltration. KBr was added to a density of 1.24 and 1.20g/cm3 respectively and two successive runs of isopynic centrifugation were performed at 32,000rpm for 48hr in a SW40 rotor ( Beckman) at 17℃. Fractions were collected from the bottom and were assayed for HBsAg. Peak fractions were pooled and dialyzed, then passed through a Sepharose 4B column ( 2.6× 100cm) . The peak fractions of HBsAg were combined and dialyzed, CsCl was added to a density of 1.20g/cm3 and a third run of isopcynic centrifugatioa were performed in CsCl. The final recovery rate is 50%-65%. HBsAg purified from cell culture supernatant of B43 consisted of homogeneous 22-nm particles under the electron microscope. The amounts of residual calf serum and residual cellular DNA were less than 30ng/dose and 31.25pg/dose respectively. The quality of pure HBsAg is over the standards set by WHO.

研究了高效表达HBsAg的CHO-B43细胞系经转瓶培养所获液体的纯化流程,具体步骤是:细胞培养液经7 500r/min 4℃离心30分钟,除去脱落细胞及碎片,加固体硫酸铵达50%饱和度进行沉淀,离心后弃上清,再用45%的饱和硫酸铵重新悬浮,离心,保留沉淀物;正压超滤透析后,分别选用1.24g/cm~3和1.20g/cm~3的KBr浮力密度,相继进行两次32 000r/min 17℃ 46小时的平衡密度超速离心,收集HBsAg活性部分,过Sepharose 4B分子筛层析柱,再收集HBsAg活性峰,进行最后一次1.22g/cm~2 CsC1平衡密度梯度超速离心。由此流程HBsAg最终回收率为50%~65%,所获纯品纯度达95%以上,经电镜检查,牛血清残余量(<8.430ng/Dose)和细胞DNA残余量(<32pg/Dose),均符合WHO规定的基因工程HBsAg人体接种的标准。

The physical, chemical and biologic properties of hepatitis B virus surface antigen ( HBsAg ) secreted by mammalian cells were studied. The buoyant density of HBsAg in CsCl is 1.21g/cm3. The pure HBsAg appeared as 3 peaks through FPLC Superose HR 6 column, one big peak and two small ones, indicating minor variation in particle size. All of them are specific HBsAg. Only one symmetric peak appeared through FPLC Mono Q column which shows that the electric charge of HBsAg particles is homogeneous. Scanning analysis...

The physical, chemical and biologic properties of hepatitis B virus surface antigen ( HBsAg ) secreted by mammalian cells were studied. The buoyant density of HBsAg in CsCl is 1.21g/cm3. The pure HBsAg appeared as 3 peaks through FPLC Superose HR 6 column, one big peak and two small ones, indicating minor variation in particle size. All of them are specific HBsAg. Only one symmetric peak appeared through FPLC Mono Q column which shows that the electric charge of HBsAg particles is homogeneous. Scanning analysis of HBsAg polypeptides in SDS-pAGE after silver staining indicated that the percentages of p23, gp27 and gp30 were 65%, 20%, 10% respectively in addition to 5% dimer. The N-terminal amiao acid sequence of HBsAg is the same as the sequence coded by trans-fected gene. HBsAg is stable at 4℃ and -20℃ but not stable at room temperature for long time. The immunogenicity test in mice showed that cellular HBsAg was 1.55-2.64 times more potent than that blood-derived HBsAg. HBsAg treated with 0.025% formalin produced higher HBsAb than that without formalin. The above data indicate that HBsAg from mammalian cells are suitable for vaccine purpose.

研究了哺乳动物细胞分泌的乙型肝炎病毒表面抗原(HBsAg)纯品的理化及生物学性状。结果表明:此种HBsAg在CsCl中的浮力密度是1.21g/cm~3;快速液相层析的SuperoseHR6层析柱上呈现3个峰,中间是一个主峰,两侧各一小峰;经Mono Q柱层析呈现一个对称峰,证明了HBsAg颗粒所带电荷的均一性;以SDS-PAGE和凝胶扫描方法分析HBsAg的多肽,P23、gp27和gp30各占65%、20%和10%,另有5%的二聚体存在;N-末端的氨基酸序列与转入细胞的目的基因所编码的序列相同;HBsAg在4℃和-20℃储存较稳定,室温条件保存时间不宜过长。动物实验证明:用与血源HBsAg疫苗同等剂量的基因工程HBsAg疫苗接种Balb/C小鼠,可获得比血源疫苗高2.64倍的免疫效果。此外,经过福尔马林处理的疫苗较未处理的疫苗有较强的免疫原性。

 
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