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gem基因
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  gem gene
     Changes of GEM Gene Expression During 3T_3-L_1 Preadipocyte Differentiation
     GEM基因在3T_3-L_1脂肪细胞诱导分化过程中的表达变化
短句来源
     Objective To investigate the changes of GEM gene expression during 3T_3-L_1 preadipocyte differentiation and to explore the relationship between GEM gene and the etiology of obesity.
     目的观察3T3L1前体脂肪细胞诱导分化过程中GEM基因表达水平的变化,探讨GEM基因与肥胖发生的关系。
短句来源
     Results During the differentiation of 3T_3-L_1 preadipocytes,the expression level of GEM gene mRNA didn't change significantly(P>0.05).
     结果在3T3L1脂肪细胞的诱导分化过程中,GEM基因mRNA表达水平无显著变化(P>0.05)。
短句来源
     Conclusion Further studies may be needed to explore the role of GEM gene in 3T_3-L_1 preadipocyte differentiation as wells as lipid synthesis and accumulation.
     结论GEM基因在脂肪细胞分化、脂质合成和积聚过程中的作用有待进一步研究。
短句来源
  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     coli lac Z gene.
     colilacZ基因
短句来源
     The OPG gene was cloned into pEGM3zf vector and sequenced.
     将该基因克隆到 p GEM3zf-载体中 ,测序鉴定 .
短句来源
     The fragment was successfully amplified by PCR and was cloned into pGEM T vector.
     利用 PCR法成功地扩增了该基因 ,并克隆入载体 p GEM-T。
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  gem gene
On the basis of the identification of this mechanism of action, we propose to change the name of the Mu lig gene (thought originally to be the structural gene for a bacteriophage ligase) to gem (gene expression modulation).
      
Based on the marked inhibitory effect of WT Gem on ICa,L, complete AV block might have been a potential consequence of WT Gem gene transduction.
      
Focal modification of AV nodal conduction by WT Gem gene transfer in swine hearts.
      
We therefore decided to check whether KIF9 and Gem gene expression patterns were compatible with each other.
      
We therefore investigated the effects on other ion currents such as IK1, INa, and ICa,T before and after Gem gene transfer.
      


Objective To investigate the mechanisms of the drug resistance of gemicitabine resistance variant of the human lung adenocarcinoma cell line A549-Gem.Method Immunohistochemistry and RT-PCR were used to tested the expression of P-glycoprotein and transcription of mRNA of multidrug resistance gene and deoxycytidine kinase. Using a cDNA microarray compared the expression profiles between the resistant A549-Gem and the parent cell line A549.Results The A549-Gem shown slight positive expression of P-glycoprotein...

Objective To investigate the mechanisms of the drug resistance of gemicitabine resistance variant of the human lung adenocarcinoma cell line A549-Gem.Method Immunohistochemistry and RT-PCR were used to tested the expression of P-glycoprotein and transcription of mRNA of multidrug resistance gene and deoxycytidine kinase. Using a cDNA microarray compared the expression profiles between the resistant A549-Gem and the parent cell line A549.Results The A549-Gem shown slight positive expression of P-glycoprotein compared with positive control by immunohistochemistry, but A549 was negative for P-gp. RT-PCR amplified mRNA, using specific dCK and mutidrug resistant gene 1(mdr1) primers, demonstrated that A549-Gem express amplicon of mdr1 but no products of mdr1 was detected in A549. In the parent cell line, dCK mRNA amplication product could be detected, but did not found in resistant cells. About 18.8% of the total DNA elements had substantially altered level of expression in resistant A549-Gem compared with A549 by cDNA microarray. The biochemical functions of the different expressed genes are diverse and include oncogene and tumor supressor gene, cell cycle regulator, heat shock protein, apoptotic and antiapoptotic factors, DNA transcription factors, DNA repair and recombination factors, signal transduction genes, protein translation genes, as well as a large number of metabolic genes. Several cluster genes overexpressed in resistant cell line, including ubiquitin-proteasome system, zinc finger protein, glutaredoxin and heat shock protein, may be related to the mechanisms of gemcitabine resistance.Conclusion The mechanisms of resistance to gemcitabine are multifactorial, may include multidrug resistance and dCK deficiency. Differential expression genes monitored by cDNA microarray between A549-Gem and A549 may be related to the mechanisms of gemcitabine resistance in human lung cancer and potentially suggest the prevalence of expression of these genes in drug resistance. The use of cDNA microarray has provided a global view of the response of lung cancer cells to gemcitabine at the genomic level, it should be suitable for examining the development of drug resistance in cancer.

目的 研究人肺腺癌吉西他滨耐药细胞系A5 4 9 Gem的耐药机制。方法 免疫组织化学法检测人肺腺癌吉西他滨耐药细胞系的P 糖蛋白的表达 ,逆转录 聚合酶链反应 (RT PCR)法检测多药耐药基因 (mdr1)以及脱氧胞苷激酶 (dCK)mRNA的转录 ,并采用基因芯片检测了A5 4 9和A5 4 9 Gem的基因表达谱。结果 A5 4 9 Gem中P 糖蛋白 (P gp)呈弱阳性表达 ,A5 4 9未见表达。A5 4 9 Gem细胞中出现mdr1的mRNA转录增加 ,却未检测到dCKmRNA的产物。基因芯片检测A5 4 9和A5 4 9 Gem基因表达谱 ,结果表明 ,18.8%的基因出现差异表达 ,包括癌基因和抑癌基因、细胞周期相关基因、热休克蛋白基因、细胞凋亡相关基因、DNA转录因子 (如锌指蛋白等 )、DNA修复和重组基因、信号传导基因、蛋白翻译基因以及大量的代谢相关基因。结论 肺腺癌细胞对吉西他滨的耐药既与dCK缺乏相关 ,也有mdr1/P gp高表达的参与。在肺腺癌细胞对吉西他滨产生耐药的过程中 ,发生了多种基因表达的变化 ,提示耐药的产生是多因素参与的复杂生化过程。

Objective To investigate the changes of GEM gene expression during 3T_3-L_1 preadipocyte differentiation and to explore the relationship between GEM gene and the etiology of obesity.Method 3T_3-L_1 preadipocytes were cultured in vitro and differentiated into the matured adipocytes.Total RNA was extracted from adipocytes of various times and the levels of GEM gene mRNA expression were evaluated by RT-PCR.Results During the differentiation of 3T_3-L_1 preadipocytes,the expression level of GEM gene mRNA didn't...

Objective To investigate the changes of GEM gene expression during 3T_3-L_1 preadipocyte differentiation and to explore the relationship between GEM gene and the etiology of obesity.Method 3T_3-L_1 preadipocytes were cultured in vitro and differentiated into the matured adipocytes.Total RNA was extracted from adipocytes of various times and the levels of GEM gene mRNA expression were evaluated by RT-PCR.Results During the differentiation of 3T_3-L_1 preadipocytes,the expression level of GEM gene mRNA didn't change significantly(P>0.05).Conclusion Further studies may be needed to explore the role of GEM gene in 3T_3-L_1 preadipocyte differentiation as wells as lipid synthesis and accumulation.

目的观察3T3L1前体脂肪细胞诱导分化过程中GEM基因表达水平的变化,探讨GEM基因与肥胖发生的关系。方法体外培养3T3L1前体脂肪细胞,采用RTPCR技术检测分化不同时段的3T3L1脂肪细胞中GEM基因的mRNA表达水平。结果在3T3L1脂肪细胞的诱导分化过程中,GEM基因mRNA表达水平无显著变化(P>0.05)。结论GEM基因在脂肪细胞分化、脂质合成和积聚过程中的作用有待进一步研究。

 
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