The medium consisted of following chemicals:25 ml soil extract,1.5 g KH\-2PO\-4,4 g K\-2HPO\-4,0.2 g sodium urate,1 g sodium galacturonic acid(sodium pectate),1 g sorbose,2 ml Dox salt,1 ml Tergitol NP-10,17 g agar,and 0.1 g PCNB in 1 liter of medium(pH adjusted to 6.4～6.7).
After treatment with Benzbromarone, the arthritis disappeared and the serum urate acid decreased to normal [from (540.6?112.8) jmnol/L to (252.0?3.2) nmol/L, P<0.01]. The FRUAC increased from(6.6?.7)% to (14.4 ?8.2)% (P<0.01). The change of Cr was not significant.
The results indicated that VA levels had significant effects on feed intake( P <0.05), laying rate( P <0.05) and serum glucose( P <0.01), but no significant effects on the rate of feed and egg, shell thickness, egg weight, serum urea acid and antibody levels under high temperature( P >0.05).
The relative standard deviation(RSD.) is less than 3.8%(n=5) and the recovery is in the range of 99%～104% for the determination of medicament, and dopamine,ascorbic acid and urea acid do not interfere the quantitative analysis of epinephrine.
Blood pressure, fasting blood sugar, body mass index (BMI), waist/hip ratio(WHR), TG, TC and LDL-C were significantly higher in the hyperuricemic group than in normal group and these parameters were strongly related to serum uric acid levels.
The goal of this research was to measure in vitro the inhibitory constants of the antioxidants ascorbic and uric acid in urine, with lucigenin enhanced chemiluminescence (CL) in Fenton's system.
Ascorbic acid exhibits up to 4-fold stronger antioxidant effect than uric acid.
The constants of antioxidant inhibition in urine were measured at concentrations 10-3 and 10-4 M: for ascorbic acid, 5.92 ± 0.04 and 24.05 ± 1.82 μmol·sec-1; for uric acid, 1.60 ± 0.02 and 21.45 ± 0.97 μmol·sec-1, respectively.
Inhibition of xanthine oxidase-catalyzed conversion of xanthine to uric acid by various pyrazolopyrimidine-based inhibitors (allopurinol derivatives) was evaluated and compared with the standard inhibitor allopurinol.