Comparitive analysis of genomic sequences of equine infectious anemia donkey leucocyte attenuated virus and its parental virulent virus (strain L) and construction of molecular clone derived from attenuated EIAV
Designing a pair of primers according to NADL-2 strain of PPV from the Genbank,the major antigen regions of VP2 of NJ-1 strain were amplified by PCR. The PCR products were cloned into pGEM-T Easy vector. 882 bp nucleotide sequence was acquired,and 294 amino acid sequence was deduced.
In order to clarify the structure of duck major histocompatibility complex MHC classⅠ(MHCⅠ), duck MHC classⅠ light chain cDNA (Anpl -β2m, GenBank accession No.AB246408) was cloned from a duck cDNA library by Full RACE PCR.
The S1 gene of Infectious bronchitis virus(IBV) was segmented and cloned into the expression vector pGEX-6p-1 by gene recombination technology. The glutathiones transferase ( GST) fusion proteins were induced by IPTG.
A luxR homologue, designated as xooR, was cloned from X.
Two DREB-like genes from Bermuda grass that are induced by low-temperature or high-salt stresses were cloned using RT-PCR and RACE methods, and were named BeDREB1 and BeDREB2, respectively (GenBank accession No: AY462117 and AY462118).
The sequence cloned was 99% homologous to Rhodococcus erythropolis IGTS8 that was reported in the Genebank.
Though inter-species nuclear transfer has been introduced to construct human somatic cell cloned embryos, the effects of type, passage, and preparation method of donor cells on embryo development remain unclear.
In our experiment, cloned embryos were reconstructed with different passage and preparation methods of ossocartilaginous cell, skin fibroblast, and cumulus cells.
In order to identify the optimum cutting density for producing the highest number of plantable seedlings of poplar clones, a split-plot randomized block design was used to establish four cutting densities in plots.
Results indicated that the leaf area, stem and leaf biomass, and caliper of seedlings of all four poplar clones increased with the decrease in cutting density.
The quantity of first-grade seedlings (grade I) for clones Nanlin-95 and Nanlin-895 was achieved at the spacing of 40 cm×50 cm; for NL-1388 and NL-80351, it was 50 cm×50 cm.
According to the seedling quality and the number of plantable seedlings produced, the suggested cutting density for these four poplar clones was 50,000 stems/hm2.
Foliar Carbon Isotope Composition (δ13C) and Water Use Efficiency of Different Populus deltoids Clones Under Water Stress