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scar引物
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  scar primers
     Polymorphism of parentswas analysized with 128 pairs of AFLP primers, 16 SSR primers and 3 SCAR primers.
     利用128对AFLP引物组合、16对ssR引物、3对scAR引物对亲本进行多态性分析。
短句来源
     (2) Selecting 66 RAPD primers out of 600. Among the 66 primers, 5 RAPD primer to SCAR primer were successfully transformed to SCAR primers.
     (2) 从600条10bp的RAPD引物中筛选获得66条多态性引物,并从中挑选了5个RAPD引物,成功地进行了向SCAR引物的转化。
短句来源
     3 pairs of species-specific SCAR primers are designed based on the DNA sequences, which are SCB_1/SCB_2, SCN_1/SCN_2, SCX_1/SCX_2. All samples of three Larch species are re-tested by these SCAR primers and good result is obtained, even though the percentage of the band's appearance is not in 100% that probably causes by the mutations in primer site among individuals of a species.
     将用于落叶松种子鉴别的三个RAPD物种特异片段回收、克隆后进行测序,根据测序片段两端的序列设计出3对物种特异的SCAR引物,分别为SCB_1/SCB_2; SCN_1/SCN_2;
短句来源
     successfully converted two unique segments to SCAR marker and designed two pairs of SCAR primers;
     将这两个特异片段转化为SCAR标记并设计两对SCAR引物;
短句来源
     The three specific fragments were cloned and sequenced, and three pairs of SCAR primers were designed according to the sequences.
     将3个种群的特异性片段分别克隆和测序,根据测序结果设计3对SCAR引物
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  scar primer
     (2) Selecting 66 RAPD primers out of 600. Among the 66 primers, 5 RAPD primer to SCAR primer were successfully transformed to SCAR primers.
     (2) 从600条10bp的RAPD引物中筛选获得66条多态性引物,并从中挑选了5个RAPD引物,成功地进行了向SCAR引物的转化。
短句来源
     SCAR primer combination SCAR_(rl) polymorphic bands about 140bp linked to YrGA gene with the genetic disease about 0.355± 0.001cM.
     SCAR引物SCAR_(s2018RL-140)标记位点与抗条锈病基因YrGA之间的遗传距离为:0.355±0.001cM;
短句来源
     Used different sex type cucumber as materials, the relationship between CsACS1G and F alleles and gynoecious characters are analyzed by specific SCAR primer of CsACS1G.
     以不同性别类型的黄瓜为研究材料,通过针对CsACS1G基因设计的特异SCAR引物分析了CsACS1G基因与F基因以及雌性性状之间的关系,并对CsACS1G进行了定位。
短句来源
     With improvement of the conditions of PCR, the specific fragment of B biotype, non-B ZHJ-1 and non-B ZHJ-2 populations, namely, 439, 366 and 1238 nt, respectively, could be amplified with the SCAR primer of the corresponding population, while the specific fragments of the other populations of B. tabaci or Trialeurodes vaporariorum could not be amplified.
     通过PCR反应条件的优化,B型、非B型ZHJ-1和非B型ZHJ-23个种群各自的SCAR引物分别能扩增出本种群的特异性条带,其大小依次为439、366和1238bp,而其它种群与温室白粉虱(Tri-aleurodesvaporariorum)则无扩增。
短句来源
     (4) The NILS and its F2 population ware screened using SCAR primer.
     (4)利用设计的SCAR引物进行PCR的扩增,稳定地重现了上述标记,结果把RAPD标记转化成为较稳定的SCAR标记—SCOPD06.1530。
短句来源
  “scar引物”译为未确定词的双语例句
     Positive colony was identified for sequencing. According to the sequences, three pairs of 22-mer specific primers, SCS13L/SCS13R, SCS200L/SCS200R1 and SCS200L/ SCS200R2 were designed.
     根据测序结果,设计了3对SCAR引物:SCS13L/SCS13R(S132800的SCAR标记引物)、SCS200L/SCS200R1、SCS200L/SCS200R2(S2002400的SCAR标记引物)。
短句来源
     By means of Carte's classified criterion, three type of special bands including Type1,Type2,Type3.
     SCAR引物扩增产物分为Type1、Type2、Type5等3种带型。
短句来源
     Results: This test developed a pair specific primers of P4.1 and P4.2.Using the specific primers P4.1 and P4.2 in the PCR,only 6 kava materials amplified the 562bp specific band,the others were not any amplified.
     结果:研究开发了1对卡瓦胡椒特异SCAR引物P4.1和P4.2,用这对特异引物对试验的28份材料进行PCR扩增,结果只有6份卡瓦胡椒材料扩增出了预期大小562bp的特异带,其它材料均无任何扩增。
短句来源
     RAPD technique was used to test the 31 cultivated strains of Lentinula edodes. Four RAPD specific bands were selected, cloned and sequenced, then used as bases for designing SCAR markers, shengxiang 93F/R,45F/R, 135F/R and 507F/R, respectively.
     本文用120个随机引物(S1-S120)对31个香菇生产用标准菌株进行RAPD扩增,筛选获得四个 RAPD特异标记,回收特异性条带,经克隆测序后设计了四对SCAR引物,分别为申香93F/R,45 F/R,135 F/R和507F/R。
     The selected primers including 54AFLP, 3 SSR and 3 SCAR with rich polymorphism and steady bands were tested in progeny. 173Markers were scored , of which 44markers were skewed segregation by ?
     选择扩增稳定、多态性丰富的引物(包括54对AFLP引物组合、3对ssR引物、3对scAR引物)进行群体分离分析,获得分离标记共173个,卡方检验44个标记发生偏分离。
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  scar primers
A species-specific random amplified polymorphic DNA (RAPD) marker was identified, cloned, and sequenced, and sequence characterized amplified region (SCAR) primers for specific PCR were developed.
      
This increases the reliability of the designed system of SCAR primers, because plasmids may be lost or transferred by transformation between closely related strains.
      
The developed sets of SCAR primers were successfully used in straightforward, fast and reliable PCR assays to identify M.
      
All pairs of SCAR primers resulted in the amplification of single major bands the same size as the RAPD fragment cloned.
      
Five out of eight pairs of SCAR primers amplified an alternate allele from both parents of the mapping population; therefore, the original RAPD polymorphism was likely due to mismatch at the primer sites.
      
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  scar primer
This granted one marker amplified with the internal SCAR primer set OPF141083 the ability to differentiate between parental individuals carrying the Dn5 genes.
      
Resistant parent G 73 and resistant bulk amplified single specific band on PCR amplification using SCAR primer pairs.
      
The SCAR primer pair ISPJ1 amplified a 543?bp fragment in all the Indian populations, while ISPJ2 with a specific amplicon of 1,096?bp was specific to the Mexican genotype.
      
We developed and tested two SCAR primer pairs that are associated with the mode of reproduction in P.?pratensis.
      
All SCAR primer pairs resulted in the amplification of specific fragments.
      


Two RAPD markers, OPR7936 and OPR17524, which closely linked to Ph1 gene in Chinese Spring wheat, were identified by using 260 10-mer random primers for the total DNAs of Chinese Spring (CS), ph1b mutant and the F2 population. OPR7936 and OPR17524 were located on the interstitial deletion region of Ph1 gene and distal to Ph1 gene apart from 11.4 cM and 5.0 cM respectively. Two SCAR markers were developed based on the sequences of these tWo RAPD clones. The conditions were optimal to produce only one band for...

Two RAPD markers, OPR7936 and OPR17524, which closely linked to Ph1 gene in Chinese Spring wheat, were identified by using 260 10-mer random primers for the total DNAs of Chinese Spring (CS), ph1b mutant and the F2 population. OPR7936 and OPR17524 were located on the interstitial deletion region of Ph1 gene and distal to Ph1 gene apart from 11.4 cM and 5.0 cM respectively. Two SCAR markers were developed based on the sequences of these tWo RAPD clones. The conditions were optimal to produce only one band for CS, none for ph1b mutant. So the products of SCAR analyses could be detected directly by addition of ethdium bromide under UV light in order to screen ph I b homozygotes. This strategy is less time-consuming and convenient for the marker-assisted selection of Phlgene in the wheat breeding program.

用260个10bp随机引物对中国春(CS)、ph1b突变体及其F2分离群体基因组DNA进行RAPD分析,筛选出两个特异的RAPD标记OPR7936和OPR17524。经Southern分析,并结合减数分裂MI染色体配对分析,认为这两个RAPD标记位于5BL染色体ph1b基因缺失区中,可以作为ph1b基因的分子标记。经过连锁分析,测得OPR7936与Ph1基因之间的距离为11.4cM,OPR17524与Ph1基因之间的距离为5.0cM,均位于Ph1基因的远着丝粒端。测序后,经同源性比较发现这两个DNA序列可能是Ph1b基因区中的两个不同位点上的新序列,并有可能成为Ph1基因区图谱新的探针。根据这两个PAPD克隆片段的两端序列,分别设计出一对24bp的SCAR引物,可特异扩增Ph1基因的一条带(SCR7823和SCR17403),而ph1b基因缺少各自的特征带,其扩增产物无需电泳分离,只需在反应管中加入溴化乙锭,紫外灯下直接根据荧光反应即可鉴定出ph1b基因型,为今后ph1b基因的转育进行标记辅助选择又提供了两个易于操作、有效的分子标记。

A total of 100 decamer oligonucleotides were used as primers to perform random amplified polymorphic DNA (RAPD) analysis on Triticum aestivum var. Chinese Spring and Thinopyrum intermedium. Out of these primers, one could amplify a specific DNA fragment in the accession SZ of Th. intermedium. This fragment, OPF03 1291 , was cloned and sequenced. Sequence data searching in GenBank showed that this fragment had a homologous degree of 88% to a RAPD marker OPF03 1296 (GenBank accession U43516)...

A total of 100 decamer oligonucleotides were used as primers to perform random amplified polymorphic DNA (RAPD) analysis on Triticum aestivum var. Chinese Spring and Thinopyrum intermedium. Out of these primers, one could amplify a specific DNA fragment in the accession SZ of Th. intermedium. This fragment, OPF03 1291 , was cloned and sequenced. Sequence data searching in GenBank showed that this fragment had a homologous degree of 88% to a RAPD marker OPF03 1296 (GenBank accession U43516) in Th. bessarabicum. Based on the sequence of OPF03 1291 , two pairs SCAR primers were designed. With OPF03 and primers P3 and P4, RAPD and SCAR analyses were performed on T.aestivum, T.aestivum Th.intermedium partial amphidiploid, Th.elongatum, Th.intermedium, wheat Th.elongatum substitution and addition lines. The results of PCR amplification showed that the RAPD marker was not appeared in materials with genome E e, whereas SCAR marker was appeared in all E genome possessed materials. The SCAR marker derived from the E b genome specific RAPD fragment could be used in the meantime to detect the chromosome of E e genome under common wheat background.

用 10 0条 10碱基随机引物 ,以普通小麦中国春、中间偃麦草为材料进行 RAPD分析 ,筛选到一个偃麦草染色体组特异引物 OPF0 3,并从中间偃麦草中克隆了该引物的特异 DNA片段 OPF0 312 91。将该片断与比萨偃麦草中的 OPF0 312 96(Gen Bank序号 U4 35 16 )比较 ,同源性为 88%。根据 OPF0 312 91的序列 ,设计了 2对SCAR引物 ,利用 OPF0 3和引物 P3、P4对普通小麦、普通小麦 -中间偃麦草的部分双二倍体、长穗偃麦草、中间偃麦草、小麦 -二倍体长穗偃麦草代换系、附加系共 6类材料进行了 RAPD和 SCAR分析 ,发现 RAPD标记OPF0 312 91没有出现在含 Ee染色体组的材料中 ,而标记 SCAR982 则出现于所有含 E染色体组的材料中。说明 ,根据 Eb 染色体组特异 RAPD标记转化的 SCAR标记 ,可以同时检测小麦背景下的 Ee 染色体

To develop a rapid and sensitive method for the detection and identification of Meloidogyne incognita, M. javanica and M. arenaria, four and three random amplified polymorphic DNA (RAPD) fragments specific for M. incognita and M. javanica, respectively, were identified. Based on the sequences of these RAPD markers, various sequence characterized amplified region (SCAR) primers were designed and tested for their amplification specificity and efficiency against populations of M. incognita, M. javanica, M. arenaria,...

To develop a rapid and sensitive method for the detection and identification of Meloidogyne incognita, M. javanica and M. arenaria, four and three random amplified polymorphic DNA (RAPD) fragments specific for M. incognita and M. javanica, respectively, were identified. Based on the sequences of these RAPD markers, various sequence characterized amplified region (SCAR) primers were designed and tested for their amplification specificity and efficiency against populations of M. incognita, M. javanica, M. arenaria, M. hapla and M. enterolobii. This resulted in three pairs of SCAR primers that were used in combination to reliably and sensitively identify M. incognita, M. javanica and M. arenaria. The SCAR markers can be readily amplified from one third of a single second-stage juvenile, male or female, thus demonstrating that the SCAR-based PCR assays have practical value for routine identification of M. incognita, M. javanica and M. arenaria in soil and root samples.

为了研制南方、爪哇和花生根结线虫快速灵敏的检测和鉴定方法 ,分别分离了 4个南方根结线虫和 3个爪哇根结线虫特异性的随机扩增多态性 DNA( RAPD)片段。在这些 RAPD标记 DNA序列的基础上 ,设计了多对 SCAR PCR引物 ,并用源于国内外的南方、爪哇、花生、北方和象耳豆根结线虫群体验证其扩增特异性和灵敏度。最终确定了 3对高效扩增的SCAR引物 ,它们组合使用可以可靠灵敏地鉴定南方、爪哇和花生根结线虫。 3对引物的扩增灵敏度达 1 / 3条的二龄幼虫、雄虫或雌虫 ,这表明本研究研制的 PCR鉴定法可用于生产实践中土样和根样中 3种根结线虫快速灵敏的鉴定。

 
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