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scar分析
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  scar analysis
     The SCAR analysis of DH population and 78 F2 plants displayed that the result of amplification with SCAR is identical with that of RAPD. The reliability of result showed that the marker was converted into SCAR marker successfully.
     分别对DH群体及78 F2单株进行SCAR分析,其扩增结果与RAPD标记OPB01-845在DH群体及78 F2单株中的分离相一致,表明该RAPD标记已成功地转化为SCAR标记。
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  “scar分析”译为未确定词的双语例句
     Analysis F_3 Individuals(BTAM428×ICS-12B) and Other Related Cultivars with SCAR
     对高粱BTAM428×ICS-12B杂交F_3代及部分抗感品种的SCAR分析
短句来源
     SCAR analyses of the twenty isolates indicated that the six types contain almost identical sequences of about 380bp and the differences among the six types were mainly due to variation within the regions from nucleotides 79 to 168 and 440 to 526. There were only several single nu-cleotide mutations in the sequences outside of these two regions.
     SCAR分析显示,特异性PCR产物类型是由于DNA序列的79-168和440-526区的核苷酸发生变化造成的,而其余380个核苷酸几乎完全一致,仅几个核苷酸位点发生了变化。
短句来源
     The results showed that the candidate marker OPK-17 550 and OPK-17 1500 were possibly correlated with the CMS gene in the line 93-A, and the candidate marker OPH-11 2000 was possibly associated with the maintainer gene in the line 93-B. The marker OPH-17 550 was cloned and partially sequenced, which provided a basis of further marker verification by SCAR marker and bulked segregation analysis.
     对侯选标记OPK 1 7550 进行了克隆和部分序列分析 ,为利用混合群体分离法 (BSA)对分离群体中的不同单株进行SCAR分析的标记验证工作奠定了基础。
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  相似匹配句对
     , G.
     分析,G.
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     After the analysis of the characteristic and advantage of .
     在分析了.
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     SCAR marker linked to seedless genes in grapes and southern blot analysis
     葡萄无核基因的SCAR标记及Southern blot分析
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  scar analysis
SCAR analysis of the F2 population and other resistant and susceptible South African wheat cultivars corroborated the observed linkage of the RAPD markers to the Dn2 resistance locus.
      
External Rhinoplasty for the Arabian Nose: A Columellar Scar Analysis
      
dhumnadeswere identified and the sequences of these bands were used to design polymerasechain reaction primers for sequence characterized amplified region (SCAR)analysis of the three snakes.
      
A multiplex SCAR analysis was established toauthenticate the snakes used in Chinese medicine reliably and efficiently.
      
SCAR analysis of theF2 population confirmed thatOPN11980/1070 and SCN11980/1070 areat the same locus linked to the SMVresistance gene.
      
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A total of 100 decamer oligonucleotides were used as primers to perform random amplified polymorphic DNA (RAPD) analysis on Triticum aestivum var. Chinese Spring and Thinopyrum intermedium. Out of these primers, one could amplify a specific DNA fragment in the accession SZ of Th. intermedium. This fragment, OPF03 1291 , was cloned and sequenced. Sequence data searching in GenBank showed that this fragment had a homologous degree of 88% to a RAPD marker OPF03 1296 (GenBank accession U43516)...

A total of 100 decamer oligonucleotides were used as primers to perform random amplified polymorphic DNA (RAPD) analysis on Triticum aestivum var. Chinese Spring and Thinopyrum intermedium. Out of these primers, one could amplify a specific DNA fragment in the accession SZ of Th. intermedium. This fragment, OPF03 1291 , was cloned and sequenced. Sequence data searching in GenBank showed that this fragment had a homologous degree of 88% to a RAPD marker OPF03 1296 (GenBank accession U43516) in Th. bessarabicum. Based on the sequence of OPF03 1291 , two pairs SCAR primers were designed. With OPF03 and primers P3 and P4, RAPD and SCAR analyses were performed on T.aestivum, T.aestivum Th.intermedium partial amphidiploid, Th.elongatum, Th.intermedium, wheat Th.elongatum substitution and addition lines. The results of PCR amplification showed that the RAPD marker was not appeared in materials with genome E e, whereas SCAR marker was appeared in all E genome possessed materials. The SCAR marker derived from the E b genome specific RAPD fragment could be used in the meantime to detect the chromosome of E e genome under common wheat background.

用 10 0条 10碱基随机引物 ,以普通小麦中国春、中间偃麦草为材料进行 RAPD分析 ,筛选到一个偃麦草染色体组特异引物 OPF0 3,并从中间偃麦草中克隆了该引物的特异 DNA片段 OPF0 312 91。将该片断与比萨偃麦草中的 OPF0 312 96(Gen Bank序号 U4 35 16 )比较 ,同源性为 88%。根据 OPF0 312 91的序列 ,设计了 2对SCAR引物 ,利用 OPF0 3和引物 P3、P4对普通小麦、普通小麦 -中间偃麦草的部分双二倍体、长穗偃麦草、中间偃麦草、小麦 -二倍体长穗偃麦草代换系、附加系共 6类材料进行了 RAPD和 SCAR分析 ,发现 RAPD标记OPF0 312 91没有出现在含 Ee染色体组的材料中 ,而标记 SCAR982 则出现于所有含 E染色体组的材料中。说明 ,根据 Eb 染色体组特异 RAPD标记转化的 SCAR标记 ,可以同时检测小麦背景下的 Ee 染色体

Three hundred and seventy eight isolates of Magnaporthe grisea were collected from 17 provinces (cities) in China and their geographic distribution of mating type and its fertility was tested with 4 standard isolates, KA3 and TH12 (Mat1.1) and Guy11 and TH16 (Mat1.2) provided by CIRAD. Seventy three fertile isolates of them were tested with polymorphic SCARs analysis by use PCR markers of 13 primer pairs. Preliminary results showed that the geographic distribution of M. grisea existed among isolates from the...

Three hundred and seventy eight isolates of Magnaporthe grisea were collected from 17 provinces (cities) in China and their geographic distribution of mating type and its fertility was tested with 4 standard isolates, KA3 and TH12 (Mat1.1) and Guy11 and TH16 (Mat1.2) provided by CIRAD. Seventy three fertile isolates of them were tested with polymorphic SCARs analysis by use PCR markers of 13 primer pairs. Preliminary results showed that the geographic distribution of M. grisea existed among isolates from the same location as well as different location and its relative relationship between fertile isolates of the fungus in China. The existence of sexual reproduction of M. grisea was explored in the field as well.

用国际 4个稻瘟病菌的标准菌株 ,测定我国 17省、市 378个稻瘟病菌的交配型及其能育菌株的地理分布 ,并用 13对引物的PCR产物对其 73个能育菌株进行SCARs分析 ,初步明确了我国稻瘟病菌交配型的地理分布及其能育菌株的亲缘关系 ,并对该菌有性生殖在田间存在的可能性进行了讨论。

decamer random primers were used to amplify between susceptible parent Yumai 13 and resistant parent Timgalen with Pm6 gene, and 37 primers of them could detect the polymorphic segment. Through several repeat and confirmation of small F 2 population derived from a cross between Timgalen and Yumai 13,one primer S138 produced reproducible segment only in the resistant parent Timgalen. The segment was recovered, cloned and sequenced. According to its sequence, a pair of special primer was synthesized and the...

decamer random primers were used to amplify between susceptible parent Yumai 13 and resistant parent Timgalen with Pm6 gene, and 37 primers of them could detect the polymorphic segment. Through several repeat and confirmation of small F 2 population derived from a cross between Timgalen and Yumai 13,one primer S138 produced reproducible segment only in the resistant parent Timgalen. The segment was recovered, cloned and sequenced. According to its sequence, a pair of special primer was synthesized and the RAPD marker was converted into a SCAR marker. Linkage analysis on the single plant of F 2 population, showed that SCAR 443 markers were linked in coupling to the resistance genes Pm6 at a distance of 27.1 cM. The marker SCAR 443 was use to screen a set of varieties carrying different Pm genes and its value was evaluated.

用 1 8 1条 1 0碱基随机引物扩增感病亲本豫麦 1 3号和含Pm6基因的抗病亲本Tim galen ,有 37条引物在Timgalen中检测到多态性片断 ,经多次重复和F2 验证 ,引物S1 38仍能在Timgalen中扩增出稳定的多态性片断 ,对该多态性片断进行回收、克隆和测序 ,根据其序列合成 1对特异引物 ,成功地将RAPD标记转化成稳定的SCAR标记 ,并用此引物对豫麦 1 3号和Timgalen的杂交F2 单株检测 ,计算出该标记与抗病基因之间的遗传距离为 2 7.1cM。并对含不同抗白粉病基因的载体品种进行了SCAR分析

 
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