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诊断与鉴定
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  identification and typing
Identification and typing of the organisms were performed on the basis of microscopic and cultural characteristics and inhibition of growth by specific antibodies.
      
Evaluation of different PCR-based approaches for the identification and typing of environmental enterococci
      
The aim of the work was the evaluation of different PCR-based methods to found an appropriate identification and typing strategy for environmental enterococci.
      
The results obtained in this work attest the importance of PCR-based methods for identification and typing of environmental enterococci.
      
Identification and typing of members of the protein-tyrosine phosphatase gene family expressed in mouse brain
      
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The genomic DNA of 4 single drug resistant strains and 1 sensitive strain of Eimeria tenella were analysed by RAPD(Random Amplified Polymorphic DNA),the results demonstrated that the similarity coefficients(SI) between all strains were over 99 0%;RAPD-PCR fingerprints generated with primer 5' AGTCAGCCAC 3'(OPA03)revealed that one band with a molecular size of approximately 500 bp was present in the DNA template of the salinomycin resistant strain but were undetectable in the other four strains;RAPD...

The genomic DNA of 4 single drug resistant strains and 1 sensitive strain of Eimeria tenella were analysed by RAPD(Random Amplified Polymorphic DNA),the results demonstrated that the similarity coefficients(SI) between all strains were over 99 0%;RAPD-PCR fingerprints generated with primer 5' AGTCAGCCAC 3'(OPA03)revealed that one band with a molecular size of approximately 500 bp was present in the DNA template of the salinomycin resistant strain but were undetectable in the other four strains;RAPD PCR fingerprints of the salinomycin resistant strain and the clopidol resistant strain with primer 5' TCGGACGTGA 3'(OPH02)appeared the same polymorphic fragment around 1kb and this wasn't the case in the other three strains.These polymorphic fragments might be due to the existance of coccidial drug resistant genes and might lead to the design of diagnostic testes for Eimeria resistant genotypes.

应用RAPD技术进行柔嫩艾美耳球虫 4个单一抗药性虫株与 1个敏感株的基因组DNA多态性分析 ,发现 4个抗药性虫株及敏感株之间的相似值均大于 99% ;OPA0 3引物对抗盐霉素株扩增出了一条约 5 0 0bp的特异条带 ,OPH0 2引物对抗克球粉株和抗盐霉素株均扩增出了约 1kb的第三条主带 ,这些特异条带的出现很可能与球虫抗药性基因有关 ,有可能用于抗药性虫株的诊断与鉴定

 
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