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正常骨髓
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  normal bone marrow
     The percentages of CD117~+CD34~-CD123~+HLA-DR~- and CD117~+CD34~-CD9~+CD33~+ cells in nucleated cells were 0.066%±0.012% and 0.089%±0.066% in 20 normal bone marrow samples.
     正常骨髓中CD117+CD34-CD123+HLA-DR-细胞和CD117+CD34-CD9+CD33+细胞在有核细胞中的比例分别为(0.066±0.012)%和(0.089±0·066)%,与APL患者相比相差4个对数级;
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     Results The expression of CD11a and CD11b on AML blast cells from 51 patients were(36.476±22.641)% and(26.955±12.328)%,respectively,and the expression of CD11a and CD11b on normal bone marrow mononuclear cells were(19.625±12.328)% and(15.513±6.296)%,respectively.
     结果AML细胞上表达的CD11a[(36.476±22.641)%]和CD11b[(26.955±12.328)%]明显高于正常骨髓单个核细胞CD11a[(19.625±12.328)%]和CD11b[(15.513±6.296)%];
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     Results: RT-PCR was used to detect the expression of HLA-G1(0.1mg/mL) on the K562 cell, and the transfected K562 cell was co-cultured with normal bone marrow cells.
     结果: RT-PCR检测确定0.1mg/mL的HLA-G1表达于转染的K562细胞上,后与正常骨髓细胞共同培养。
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     RT-PCR can check up the WT1 expression of 1 K562 cell in 1000 CD34 + deleted umbilical cord blood cells in our experiment, but in the same condition, WT1 expression in 2/5samples of umbilical cord blood and 1/10 sample of normal bone marrow can also be detected.
     以去除CD34 + 细胞的脐血细胞对K5 6 2细胞倍比稀释后再以RT PCR检测WT1表达 ,当敏感度达 10 -3 时 ,1/ 10正常骨髓及 2 / 5脐带血亦可检测出WT1表达。
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     Effects of anti- CXCR4 monoclonal antibody on the expression of Bcl-2, PCNA and Fas proteins of human acute myelocytic leukemia cell line HL-60 co-cultured with normal bone marrow stromal cells
     抗CXCR_4单克隆抗体对与正常骨髓基质细胞共培养的HL-60细胞Bcl-2、PCNA、Fas蛋白表达的影响
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  normal marrow
     Results The ligation efficiency detected in leukemic cell lines NB4U937K562SHI-1Jurkat was 10.6%~32.9% and 5.2%~20.0% in normal marrow or peripheral blood cells.
     结果 白血病细胞株NB4、U937、K5 6 2、SHI 1、Jurkat的连接能力为10 .6 %~32 .9% ,正常骨髓或外周血细胞的连接能力为5 .2 %~2 0 .0 %。
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     Telomerase activity in normal marrow cells was 0-0.30 U, with a mean level of (0.11±0.08) U, in which 3 cases were considered positive according to the standard set by the Kit.
     结果发现 :正常骨髓细胞端粒酶表达范围 0 - 0 .30U ,平均 (0 .11± 0 .0 8)U ,其中仅有 3例阳性。
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     remarkbly in- crease the rate of S phase in the marrow cells of the leukemic mice and lower the rate of G2+M phase,which could make some improvements and increase in the proliferative activity of normal marrow cells;
     能明显提高模型小鼠骨髓细胞 S期的比率,降低 G_2+M 期的比率,使其正常骨髓细胞的增殖活性得到了一定的改善和提高;
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     Methods Nucleic protein extracts from leukemic cell lines and normal marrow or peripheral blood cells were incubated with linear plasmid pUC18 DNA, which could be separated by agarose gel electropherasis and ligation efficiency was detected by staining with SYBR greenⅠ.
     方法 从白血病细胞株和正常骨髓及外周血细胞中提取的核蛋白在体外与线性质粒pUC18DNA一起作用,通过琼脂糖凝胶电泳分离和SYBRgreenⅠ染色观察其连接能力。
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     Methods The authors studied LTC IC changes in CD + 34 cells from normal marrow, peripheral blood after mobilization and the cord blood under the stimulation of 6 kinds of cytokines with the Dexter cell long term culture assay.
     方法应用dexter方法,在6种细胞因子刺激下,对正常骨髓、动员后的外周血以及脐带血的CD+34细胞中的LTC-IC进行评价。
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  “正常骨髓”译为未确定词的双语例句
     The plating efficiencies of BM CFU-GM were 115.14± 87.85 (n=11), 59.81 ±68.05 (n = 9) and 25.00 ± 20.45 / 2 ×105(n = 6) on day 14, 21 and 28, respectively.
     正常骨髓CFU-GM产率:14天(n=11)、21天(n=9)、28天(n=6)分别为115.14±87.85、59.81±68.05、25.00±20.45/2×10~5,14天集落产率开始下降,21天后明显减少;
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     The WT1 expression levels in NBM were 0.001 to 0.019 with a median level of 0.008. Higher levels of WT1 expression were found in 61 of 67 (91%)newly diagnosed AML patients compared with NBMs and 37 of the 67 (55.2%) showed 100-fold higher WT1 levels than that in NBMs.
     正常骨髓WT1mRNA中位水平为0.008(0.001~0.019)。 67例初治AML患者中61例(91.0%)WT1mRNA表达高于正常水平,其中37例(55.2%)高于正常100倍以上。
短句来源
     Morphological and cytochemitry features of CD 34 + hematopoietic cells isolated from human bone marrow
     人正常骨髓 CD_(34)~+ 造血细胞的形态学与细胞化学特征
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     Apoptosis percentage of Jurkat cells cocultured with leukemic BMSCs by DNR was lower than that of Jurkat cells cocultured with normal BMSCs [(5.73±1.78)% vs (8.39±4.08)%, (P<0.05)].
     白血病骨髓基质对白血病细胞的屏蔽效应强于正常骨髓基质细胞[白血病细胞凋亡率分别是(5.73±1.78)%,(8.39±4.08)%,(P<0.05)]。
短句来源
     The Study of Apoptosis of Leukemia Cells Induced by CD34~+ Cells Transferred with Exogenous Fas Ligand
     转染外源性Fas配体基因的正常骨髓CD34~+细胞诱导U937细胞凋亡的实验研究
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  normal bone marrow
The expression level of TRF1 protein was significantly higher (P>amp;lt;0.01) in normal bone marrow ((2.217±0.462) μg/μl) than that of acute leukemia patients ((0.754±0.343) μg/μl).
      
It was confirmed that the activity of telomerase in normal bone marrow was lower than that of acute leukemia patients ((0.125±0.078) μg/μl vs (0.765±0.284) μg/μl, P>amp;lt;0.01).
      
NcAb SZ-39 have no crossreaction with lymphocyte, ABC red blood cells, white blood cells, blood platelet normal bone marrow cells, fibroblast cells and 12 normal human tissues.
      
Both c-myc and c-fes were detectable in leukemic cells as well as in immature granulocytes and erythroblasts of normal bone marrow, but the expression extent varied in different cases.
      
It has been found that MRD detection can be performed using molecular and immunophenotypic aberrancies that are present in the leukemic clone at diagnosis and not in normal bone marrow.
      
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  normal marrow
Small, focal, linear or oval areas of low signal seen against a background of normal marrow signal on short or long TR/ TE do not mimic tumor.
      
The patient population in our study was older (mean age 66 years) than the patient populations in previous papers documenting normal marrow patterns.
      
This two-part article reviews and illustrates these issues, with an emphasis on the practical application of MR imaging to facilitate differentiation of normal marrow, tumor, and treatment-related marrow changes in oncology patients.
      
Part 2 will emphasize the practical application of MR imaging to facilitate differentiation of normal marrow, tumor, and treatment-related marrow changes in oncology patients, and will review complementary MR techniques under development.
      
Two patients with lesions in the acetabulum and femur, evident on imaging, were found to have normal marrow elements without a histopathological lesion on curettage of the acetabulum and resection of the femur, respectively.
      
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The specific inhibition of ~3H-TdR incorporation in leukemic cells by cancer-suppressive factor (CSF) was observed in vitro. The CSF in this experiment were prepared from the ascites of Ehrlich aseites carcinoma.~3H-TdR incorporation in L_(7712) leukemic cells of mice significantly inhibited by both crude and purified CSF-preparations. The ~3H-TdR incorporation of human leukemic cells was also inhibited by these two preparations. The average inhibiting rate by G_(25) preparation (500/μg/ml) in 17 cases of human...

The specific inhibition of ~3H-TdR incorporation in leukemic cells by cancer-suppressive factor (CSF) was observed in vitro. The CSF in this experiment were prepared from the ascites of Ehrlich aseites carcinoma.~3H-TdR incorporation in L_(7712) leukemic cells of mice significantly inhibited by both crude and purified CSF-preparations. The ~3H-TdR incorporation of human leukemic cells was also inhibited by these two preparations. The average inhibiting rate by G_(25) preparation (500/μg/ml) in 17 cases of human leukemic cell was 69.6%, but in 10 cases of normal human bone marrow cell was 48.5% .The inhibiting efficiency of G_(25) preparation was higher in human leukemic cells than in normal bone marrow cells. An average inhibiting rate by LH 20 (200μg/ml) preparation in the 13 cases of human leukemic cells was 54.2%, but only 2.1% average inhibiting rate was found in 5 cases of normal human bone marrow cells. The difference of inhibition between leukemic and normal marrow cells was statistically significant. (P<0.005)

本实验室从艾氏腹水癌小鼠腹水液中分离出具有抑癌活性的物质,称为“抑癌因子”(Cancer Suppressive Factor,CSF)。本文以~3H-TdR参入DNA为主要指标,用白血病细胞及正常骨髓细胞为靶细胞,在体外观察两种CSF制品的抑制作用及其专一性。 实验表明,CSF的G_(25)粗制品与LH_(20)纯制品对L_(7712)小鼠白血病细胞的~3H-TdR参入均有明显抑制效应。同时,G_(25)制品(500微克/毫升)对17例白血病病人骨髓细胞~3H-TdR参入的抑制作用(平均抑制率为69.6%)略高于对10例非白血病病人正常骨髓细胞的抑制(平均抑制率为48.5%)。LH_(20)制品(200微克/毫升)对13例白血病病人骨髓细胞的平均抑制率为54.2%,但对5例非自血病病人正常骨髓细胞的平均抑制率为2.1%,经统计学处理,二者的差异有显著性(p<0.005)。

The viaointy of cryopreserved bone marrow cells used for autologous bone marrow transplantation were studied.1. The CFU_(GM) colony forming ability of normal bone marrow cells was estimated by three different culture methods. 2. Normal bone marrow samples from 11 individuals were cryopreserved in liquid nitrogen. The nucleated cell recovery rate, trypan blue exclusion rate and CFU_(GM) recovery rate were studied after different intervals of storage. It appears that the viability of CFU_(GM) did not change much...

The viaointy of cryopreserved bone marrow cells used for autologous bone marrow transplantation were studied.1. The CFU_(GM) colony forming ability of normal bone marrow cells was estimated by three different culture methods. 2. Normal bone marrow samples from 11 individuals were cryopreserved in liquid nitrogen. The nucleated cell recovery rate, trypan blue exclusion rate and CFU_(GM) recovery rate were studied after different intervals of storage. It appears that the viability of CFU_(GM) did not change much even after 50 weeks of storage. 3. After a two-step centrifugation, approximately 80±2.6% of nucleated cells could be seperated. 4. A first case of clinical trail was also reported. After one month of cryopreservation, the nucleated marrow cells from a patient with cervical carcinoma were infused back. The total infused nucleated cell count was 0.53×10~(10) and the total infused CFU_(GM) was 0.27×10~6. No untoward reactions are found after autotrasfusion.

本文旨在研究自体骨髓移植中多量骨髓的冷冻保存问题。(1)比较了正常骨髓粒系定向造血干细胞(CFU_(GM))活力的三种测试方法。在体内扩散盒培养方法中,用环磷酰胺0.3mg/g代替照射处理小鼠。(2)11例正常骨髓标本冻存于液氮并定期复温观察有核细胞回收率、锥蓝拒染率及CFU_(GM)回收率。当骨髓标本长期冻存达50周时,上述指标未见明显下降。(3)用二步离心法浓集多量骨髓的有核细胞。有核细胞可回收82.5±2.6%,所得有核细胞体积仅为原骨髓体积的三分之一左右。(4)在上述实验研究基础上,本文冻存了一例宫颈癌患者的自体骨髓。一个月后回输给患者的有核细胞总数为0.53×10~(10),CFU_(GM)总数为0.27×10~6。输注后患者的情况良好。

This paper introduces a method of preparing fetal liver cell suspension, it is simple and convenient, much cheaper and safer. In addition, we observed the total number of nucleated cells and CFU-C of fetal liver of various months; the comparison of CFU-C yield between normal marrow and the suspension; the activity of cells in suspension after short-term preservation; the cellular composition of fetal liver; the clinical transfusion of suspension etc. Finally., the method of preparing fetal liver cell suspension...

This paper introduces a method of preparing fetal liver cell suspension, it is simple and convenient, much cheaper and safer. In addition, we observed the total number of nucleated cells and CFU-C of fetal liver of various months; the comparison of CFU-C yield between normal marrow and the suspension; the activity of cells in suspension after short-term preservation; the cellular composition of fetal liver; the clinical transfusion of suspension etc. Finally., the method of preparing fetal liver cell suspension and the number of fetal liver needed when fetal liver transplantation are discussed.

本文介绍了胎肝细胞悬液的制备方法,此法简便、成本低廉、使用安全。此外,我们对不同月龄胎肝所含有核细胞总数、CFU-C总数、正常骨髓与胎肝细胞悬液CFU-C产率的比较、胎肝细胞悬液短时保存后的活性、胎肝的细胞组成、临床输注等进行了观察,并对胎肝细胞悬液的制备方法和移植时所需要的胎肝数进行了讨论。

 
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