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   纯化hbsag 的翻译结果: 查询用时:0.48秒
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纯化hbsag
相关语句
  purified hbsag
     Experiment Showed that solution containing ~(250)ppm,~(500)ppm available iodine with a 10 min contact time could inactivate Pre-S_2 and purified HBsAg.
     对HBV灭活实验表明,250ppm消毒液作用10min,使Pre-S_2转阴,500ppm消毒液作用10min可使纯化HBsAg达100%破坏。
短句来源
     Compared with highly purified HBsAg prepared from sera of carriers, maximum production of HBsAg from PLO cultures collected between 14-18 days was about 200ng/ml/105 cells.
     与纯化HBsAg此较,最高产量(14~18天)约为200ng/ml/10~5细胞。
短句来源
     Six hybridoma cell lines secreting monoclonal antibodies ( McAbs ) against HBsAg(adr) were derived from fusion of NS-1 myeloma cells with spleen cells of BALB/C mice immunized with purified HBsAg(adr).
     用纯化HBsAg致敏的BALB/C小鼠脾细胞与小鼠骨髓瘤株NS-1进行体细胞杂交,获得了6株分泌抗HBsAg McAb的杂交瘤。
短句来源
     HBsAg was purified by immunoaffinity chromatography from HBsAg(+) human Serum after heat inactivated in 0.2% farmal formalin,ammonium sulfate precipitation and treatment with 1% Triton X 100. 55.6 mg PAGE purified HBsAg with specific activity (titer/mg protein/ml) is 10 240 was obtain of from 1 000 ml HBsAg(+) human serum by this method.
     再通过免疫亲和层析纯化HBsAg。 应用上述方法从1000mlHBsAg(+)人血清中获得55.6mg聚丙烯酰胺凝胶电泳(PAGE)纯的HBsAg,其比活性(滴度/mg蛋白·ml-1)为10240。
短句来源
     The IFN induced by purified HBsAg was relatively stable to heating (56℃ for 1 hour), acidifying (pH 2 f皉 24 hours) and could not be neutralized by monoclonal! antibodies to IFNr.
     纯化HBsAg诱生的干扰素在热(56℃,1小时)及酸性(PH2,24小时)环境下相对稳定,不能被抗人γ干扰素单克隆抗体所中和。
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  “纯化hbsag”译为未确定词的双语例句
     The result showed that the optimum conditions for purification of HBsAg by hydrophobic interaction chromatography was as follows: using Butyl-Sepharose (6 ~ 8μmol/ml)with a C, funetional group or Propyl-Sepharose (20~22μmol/ml) with a C3 functional groupas hydrophobic media,and (NH4),SO4 concentration and temperature was 8% and 18~24℃respectively.
     结果表明,应用以C4为功能基的Butyl—Sepharose(6~8μmol/ml)和以C3为功能基的Propyl-Sepharose(20~22μmol/ml)疏水介质、8%(NH4)2SO4,18~24℃等条件纯化HBsAg时结果较理想。
短句来源
     Methods Three kinds of carriers (stainless steel, emulsion round plates with diameter of 1. 0cm, and cloth square plates with 1.5 × 1. 5cm2) were prepared.
     方法 用直径为1cm的原型钢片、乳胶片和1.5cm ×1.5cm麻布片制备3种载体片,在每个载体片上滴加含纯化HBsAg2μg的悬液。
短句来源
     HBsAg could be destroyed by WD Complex Iodine solution containing 500ppm av iodine after contact for 10min.
     含有效碘500ppm的溶液对纯化HBsAg作用10分钟,破坏率达100%。
短句来源
     A Simple Method for Purification of Large Scale HBsAg
     大量纯化HBsAg的简易方法
短句来源
     It was also indicated that the pure HBsAg could be inactivated by the disinfectant solution containing 100mg/l available chlorine after exposure for 2 min. , 50mg/l for 5min. , and 25mg/l for 10 rain.
     100毫克/升有效氯作用2分钟、50毫克/升有效氯作用5分钟、25毫克/升有效氯作用10分钟,对纯化HBsAg的灭活率达100%。
短句来源
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  相似匹配句对
     A Simple Method for Purification of Large Scale HBsAg
     大量纯化HBsAg的简易方法
短句来源
     PURIFICATION AND IMMUNOGENICITY OF RECOMBINANT HBsAg
     重组HBsAg纯化及动物免疫原性研究
短句来源
     Purification Process of 1,8-Cineole
     桉叶素的纯化
短句来源
     Purification of sodium dodecyl sulphate
     十二烷基硫酸钠的纯化
短句来源
     HbsAg, HbsAb;
     HbsAg、HbsAb;
短句来源
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  purified hbsag
Target cells were prepared by coincubation of mononuclear cells with purified HBsAg in 0.5 mM CrCl3.
      
The antigen specificity of the cytotoxicity was confirmed by a blocking experiment with purified HBsAg and by cold target inhibition with HBsAg or bovine serum albumin (irrelevant antigen) coupled cold target cells.
      
Two of the three patients showed lymphocyte transformation to purified HBsAg during the quiescent phase of the PAN.
      
Purified HBsag has a complex structure comprising 8 polypeptides.
      
HBBS can be selec tively and quantitatively removed by reaction with purified HBsAg and centrifugation.
      
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PLO/PRF/5 hepatoma cell line (PLO) adapted to Eagles' medium supplemented with 10% of inactivated calf serum in our laboratory was successfully passaged, and confluent growth was generally observed on the third day after seeding. The production of hepatitis B surface antigen(HBsAg)in tissue culture fluid was serially determined by solid phase radioimmunoassay. Prolonged incubation of culture in growth or maintenance media resulted in a progressive increase in HBsAg, which was maximum around 18 days, and then...

PLO/PRF/5 hepatoma cell line (PLO) adapted to Eagles' medium supplemented with 10% of inactivated calf serum in our laboratory was successfully passaged, and confluent growth was generally observed on the third day after seeding. The production of hepatitis B surface antigen(HBsAg)in tissue culture fluid was serially determined by solid phase radioimmunoassay. Prolonged incubation of culture in growth or maintenance media resulted in a progressive increase in HBsAg, which was maximum around 18 days, and then declined gradually. Compared with highly purified HBsAg prepared from sera of carriers, maximum production of HBsAg from PLO cultures collected between 14-18 days was about 200ng/ml/105 cells. The increase of HBsAg production was paralleled by cytological changes, in which there were degenerations in lysosomes and mitochondria, fusion of cells, and increase in the number of detached and non-viable rounded cells. These were in agreement with the suggestion that HBsAg production is cell cycle related to and representing a terminal event. Depletion of calf serum from media within four days of culture had no significant effect on the production of HBsAg, but after four days cells were found to be detached from flaska, followed by decreased production of HBsAg. When polysaooharides of Eadix Astragali Seu Hedysari (BAH), a Chinese herb which has been shown to prolong the life-span of human embryo lung diploid cell strain was added to culture media, the life-span of PLC, however, was not prolonged, nor was the production of HBsAg enhanced significantly.

PLC/PRF/5肝癌细胞系(PLC)经适应于本实验室含10%小牛血清的Eagles’营养液(csEagles’液),传代3天后即可长成单层。应用固相放射免疫测定法(SPRIA)动态检测产生乙型肝炎表面抗原(HBsAg)量,发观随培养天数延长,HBsAg产量增多,到18天左右达高峰。与纯化HBsAg此较,最高产量(14~18天)约为200ng/ml/10~5细胞。HBsAg产量的增多与PLC细胞出现溶酶体及线粒体退变、细胞融合及脱落死亡圆细胞数增多呈平行关系。这些现象与认为产生HBsAg是PLC生长周期末期活动的看法相符。自营养液中去除小牛血清,则细胞仅能短期维持,第4天起开始脱落,但4天内对HBsAg产量无显著影响。加入黄芪多糖培养,未能延长细胞生长寿命或明显提高HBsAg产量。

Six hybridoma cell lines secreting monoclonal antibodies ( McAbs ) against HBsAg(adr) were derived from fusion of NS-1 myeloma cells with spleen cells of BALB/C mice immunized with purified HBsAg(adr). The McAbs in both culture supernatant and ascitic fluid had high titer and formed immuno-preci-pitation lines with HBsAg. The McAbs 4C1、 4C3、3B6 were against a-determinant of HBsAg and 1B10 against d-determinant. The six McAbs all belonged to IgG1 subclass. Primary use of these McAbs have been studied.

纯化HBsAg致敏的BALB/C小鼠脾细胞与小鼠骨髓瘤株NS-1进行体细胞杂交,获得了6株分泌抗HBsAg McAb的杂交瘤。6个瘤株分泌的McAb在培养上清及接种裸鼠和BALB/C小鼠腹水中均有较高滴度,能与HBsAg在琼脂双扩散中形成免疫沉淀线,其中4C1、4C3、3B6为抗-a决定簇,1 B10为抗-d决定簇。6个McAb均为IgG1亚类。这些McAb试用于ELISA、RIA中包被固相载体和标记、以及免化定位肝癌组织内HBsAg,显示了较好的应用前景。

Purified HBsAg (P-HBsAg) was used as the inducer in the production of interferon(IFN)in mononuclear cells in peripheral blood in patients with acute viral hepatitis, chronic hepatitis and normal persons.There was no apparent difference in. the ability of production of IFN among these three groups (P>0.05) .The IFN induced by purified HBsAg was relatively stable to heating (56℃ for 1 hour), acidifying (pH 2 f皉 24 hours) and could not be neutralized by monoclonal! antibodies to IFNr.

作者以纯化HBsAg为诱生剂,在急性病毒性肝炎(18/23)、慢性肝炎(7/9)及正常人(8/9)的外周血单个核细胞培养中诱生出干扰素,但三者产生干扰素的能力无显著差别(P>0.05)。纯化HBsAg诱生的干扰素在热(56℃,1小时)及酸性(PH2,24小时)环境下相对稳定,不能被抗人γ干扰素单克隆抗体所中和。

 
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