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鸦片肽
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  opioid peptide
     THE MORPHOLOGICAL STUDY ON THE SIGNIFICANCE OF THE ENDOGENOUS OPIOID PEPTIDE IN THE SACRAL MICTURITION REFLEX
     内源性鸦片肽在骶髓排尿反射中的意义的形态学探讨—溃变法、HRP逆行标记法和免疫组化法相结合的光、电镜研究
短句来源
  “鸦片肽”译为未确定词的双语例句
     Systemic naloxone had no significant effect on the inhibi-tion produced by CP stimulation. Systemic methysergide reduced or eliminated the in-hibition in a part of the neurons tested, suggesting that 5--HT, but not opioid Peptides,is involved partially in mediation of the descending inhibitory effect originating fromthe cortex.
     静注纳洛酮对抑制作用无明显影响,静注二甲麦角新碱在部分神经元可使抑制作用明显减弱或完全消失,提示5-HT部分参与皮层下行抑制作用的实现,而内鸦片肽则否。
短句来源
     To illustrate the opioids action in uterus, β-endorphin (β-EP) was used as ligand to study its binding activity.
     为了探索鸦片肽对生殖系统的确切影响,研究了β内啡肽的子宫结合特性。
短句来源
     While developing an assay on binding of β-EP to plasma membrane in rat uterus we witnessed evidence of extensive peptide proteolysis that was quite different from the opiate receptor rich brain membranes. Uteri were removed from adult Sprague- Dawley rats, homogenized in 50mmol/L of Tris-HCl buffer. pH 7.5 (TB), centrifuged (12 000g) and the pellet was resuspended in TB containing glycerol.
     作者用Sprague-Dawley大鼠子宫制成子宫膜制剂,用~3Hβ内啡肽20mmol/L与此子宫膜制剂温育(30℃)15min及2h,以10%HAc中止反应,离心取得上清液,经Sephadex G50柱层析,发现~3Hβ内啡肽已分解为碎片,表明子宫膜制剂对β内啡肽有酶性降解作用,从而解释了以往文献报道未能显示子宫鸦片肽受体的原因。
短句来源
     In the presence of an opioid analog,DAMA(10(-13)~10(-10)mol/L),human granulocytes and Mytilus edulis immunocytes were incubated with or without ligands at 37℃ for 10 minutes and at 23℃ for 20 minutes, respectively.
     将人粒细胞和Mytilusedulis免疫细胞分别与不同浓度的类鸦片肽DAMA(10(-13)~10(-10)mol/L)或δ拮抗剂在37和23℃保温10~20min,用计算机显微图像分析法分析两种细胞的活化情况。 结果显示细胞免疫活性随DAMA浓度增加而提高,细胞发生阿米巴样变形或变长(形状因子小于0.5)。
短句来源
     Conclusion: The postmenopausal symptoms may result from endogenous opioid withdrawal after the cessation of ovarian steroid production.
     结论:(1)绝经后卵巢激素减少,内源性鸦片肽戒断可能与绝经后某些症状有关;
短句来源
  相似匹配句对
     Peptide Quantitative Structure-Activity Relationship Study
     的定量构效关系研究
短句来源
     Opium Evil
     打开国门的鸦片
短句来源
     The excitement effect was effectively blocked by the specific opiate receptor blocker-naloxone.
     鸦片受体阻断剂纳洛酮能有效地阻断吗啡和亮啡的兴奋作用。
短句来源
     Research and Preparation of High Protein and Peptide Milk Powder
     高蛋白奶粉的研制
短句来源
     Opium as a mental weapon of Japanese invasion of China
     鸦片:日本侵华的软武器
短句来源
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  opioid peptide
Variation in the analgesic activity of opioid peptide fragments in correlation with the amino acidic sequence
      
The purpose of this study was to determine whether the endogenous opioid peptide dynorphin A(1-13) has a direct effect on the heart or acts to modulate the cardiac chronotropic response to calcium, potassium, or β-adrenergic receptor stimulation.
      
The mu opioid peptide receptor (MOP) is the principal site of pharmacologic actions for most clinically important opiate drugs.
      
Chemokines and adhesion molecules orchestrate the migration of opioid peptide-containing leukocytes to inflamed tissue.
      
Ethanol induces differential alterations in opioid peptide expression in brain areas of the reward circuits, which may be linked to the reinforcing effects of ethanol.
      
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Single spinal dorsal horn neurons were recorded extracellularly with glass mi-cropipettes, and the effect of stimulation Of SI cortex or cerebral peduncle (CP) wasobserved on the long latency discharges (C--fiber evoked response) of the wide dynam-ic range (WDR) neurons induced by intense electrical skin stimulation. Inhibitionwas the predominant effect of SI cortex stimulation on the C--fiber evoked responses ofWDR neurons. The effect of CP stimulation was similar to that of SI cortex stimula-tion, except that...

Single spinal dorsal horn neurons were recorded extracellularly with glass mi-cropipettes, and the effect of stimulation Of SI cortex or cerebral peduncle (CP) wasobserved on the long latency discharges (C--fiber evoked response) of the wide dynam-ic range (WDR) neurons induced by intense electrical skin stimulation. Inhibitionwas the predominant effect of SI cortex stimulation on the C--fiber evoked responses ofWDR neurons. The effect of CP stimulation was similar to that of SI cortex stimula-tion, except that the former was much more effective than the latter. The inhibitionlasted a period of time varying from 400 ms to more than 10 min after CP stimula-tion in different neurons. Systemic naloxone had no significant effect on the inhibi-tion produced by CP stimulation. Systemic methysergide reduced or eliminated the in-hibition in a part of the neurons tested, suggesting that 5--HT, but not opioid Peptides,is involved partially in mediation of the descending inhibitory effect originating fromthe cortex.

在大鼠用玻璃微电极细胞外记录的方法,观察了刺激皮层体感Ⅰ区(SI区)和大脑脚(CP)对皮肤强电刺激诱发的脊髓背角广动力范围(WDR)神经元长潜伏期反应(C-反应)的影响。结果表明刺激SI区对背角WDR神经元C-反应的影响以抑制为主,刺激CP的作用与刺激SI区的作用相似,但刺激CP更为有效。抑制作用的持续时间在不同神经元差别很大,短者在刺激停止后仅持续400ms,长者可达10min以上。静注纳洛酮对抑制作用无明显影响,静注二甲麦角新碱在部分神经元可使抑制作用明显减弱或完全消失,提示5-HT部分参与皮层下行抑制作用的实现,而内鸦片肽则否。

To illustrate the opioids action in uterus, β-endorphin (β-EP) was used as ligand to study its binding activity. While developing an assay on binding of β-EP to plasma membrane in rat uterus we witnessed evidence of extensive peptide proteolysis that was quite different from the opiate receptor rich brain membranes. Uteri were removed from adult Sprague- Dawley rats, homogenized in 50mmol/L of Tris-HCl buffer. pH 7.5 (TB), centrifuged (12 000g) and the pellet was resuspended in TB containing glycerol. Incubation...

To illustrate the opioids action in uterus, β-endorphin (β-EP) was used as ligand to study its binding activity. While developing an assay on binding of β-EP to plasma membrane in rat uterus we witnessed evidence of extensive peptide proteolysis that was quite different from the opiate receptor rich brain membranes. Uteri were removed from adult Sprague- Dawley rats, homogenized in 50mmol/L of Tris-HCl buffer. pH 7.5 (TB), centrifuged (12 000g) and the pellet was resuspended in TB containing glycerol. Incubation at 30℃ (15 min or 2h)metabolized ~3H β-EP(20 nmol/L.) almost completely into tiny fragments of pep- tides, which was reduced at 4℃, but only about 20% of the intact peptide remained after 2 h. ~3H β-EP could be largely protected from degradation by aprotinin and PMSF. β-EP bind- ing is considerably enhanced by preincubation of the uterine membranes, thus suggesting the presence of endogenous ligands.

为了探索鸦片肽对生殖系统的确切影响,研究了β内啡肽的子宫结合特性。作者用Sprague-Dawley大鼠子宫制成子宫膜制剂,用~3Hβ内啡肽20mmol/L与此子宫膜制剂温育(30℃)15min及2h,以10%HAc中止反应,离心取得上清液,经Sephadex G50柱层析,发现~3Hβ内啡肽已分解为碎片,表明子宫膜制剂对β内啡肽有酶性降解作用,从而解释了以往文献报道未能显示子宫鸦片肽受体的原因。本文在使用丝氨酸蛋白酶抑制剂0.1mmol/L PMSF为保护剂的条件下,对子宫的β内啡肽受体进行受体分析,饱和实验表明,子宫膜悬液有β内啡肽结合效应,并对此种结合的特征作了描述和讨论。

In the presence of an opioid analog,DAMA(10(-13)~10(-10)mol/L),human granulocytes and Mytilus edulis immunocytes were incubated with or without ligands at 37℃ for 10 minutes and at 23℃ for 20 minutes, respectively.The percentage of the immune cells with con-formational changes(to elongated or ameboid shapes)in dicative of activation was determined by computer-assisted microscopic image analysis, The results showed that DAMA,in a concentration dependent manner,can induce inactive invertebrate immunocytes and...

In the presence of an opioid analog,DAMA(10(-13)~10(-10)mol/L),human granulocytes and Mytilus edulis immunocytes were incubated with or without ligands at 37℃ for 10 minutes and at 23℃ for 20 minutes, respectively.The percentage of the immune cells with con-formational changes(to elongated or ameboid shapes)in dicative of activation was determined by computer-assisted microscopic image analysis, The results showed that DAMA,in a concentration dependent manner,can induce inactive invertebrate immunocytes and human granulocytes to undergo activation with shape factors below 0.50. A delta antagonist,naltrindole,at a low concentration(10(-10)mol/L)can significantly inhibit DAMA induced activation of immune cells(P<0.05 ), indicating the blockade of the receptor by naltrindole, However,the general opioid and opiate antagonist,naloxone,is not effective at this low concentration,further suggesting that δ2 opioid receptor mediates opioid immunocyte activation.

将人粒细胞和Mytilusedulis免疫细胞分别与不同浓度的类鸦片肽DAMA(10(-13)~10(-10)mol/L)或δ拮抗剂在37和23℃保温10~20min,用计算机显微图像分析法分析两种细胞的活化情况。结果显示细胞免疫活性随DAMA浓度增加而提高,细胞发生阿米巴样变形或变长(形状因子小于0.5)。当加入δ拮抗剂naltrindole(10(-10)mol/L)时,DAMA对以上两种细胞的免疫激活作用均受到显著抑制(P<0.05),说明该类鸦片肽受体能被低浓度naltrindole封闭;然而,同样浓度的另一种δ拮抗剂钠洛酮却没有这种封闭作用,提示DAMA通过δ_2受体的介导发挥对免疫细胞的激活作用。

 
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