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血膜片
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  blood films
     Methods Based on the published literatures thick blood films and filter paper blood spot were prepared by taking finger blood from patients from malaria endemic areas and attending at the clinics using Plasmodium falciparum PF 1-2and plamodium vivax PV 3-5 as primers,and PCR test was conducted in the same reaction system,the amplified fragments are 206bp and 431bp.
     方法 对疟区求诊发热病人 ,手指取血分别制作厚薄血膜片和滤纸血斑作为检测血样。 参照文献用恶性疟原虫PF1 - 2 和间日疟原虫PV3 - 5为特异性引物 ,同在一个反应体系常规进行PCR ,扩增片段分别为 2 0 6bp和 431bp。
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  “血膜片”译为未确定词的双语例句
     (2)In the same patient at the different stages of the disease-i. e. chills,fever and intermission stages, the detection rates of Schuffner's dots in RBC infected with trophozoites were 35.4%,56.7% and 88.3% respectively.
     在同一例病人临床发作的发冷期、发热期和发作间歇期的薄血膜片,以同一浓度的吉氏液染色,薛氏点的检出率分别为35.4%、56.7%和88.3%。
短句来源
     (3)The relationship between the method for drying the thin blood film and the detection rate of multinuclear trophozoites(RTM)and of infected erythrocyte morphological change(REMC). When the thin film was dried by the electric fan(fast drying method),the RTM and REMC were 1.47% and 11.13%,and the parasitized red cells were enlarged in 58.99%,only 29.88% the infected erythrocytes appeared normal.
     (3)薄血膜片干燥快或慢与多核疟原虫和受染红细胞形态变化的检出率有显著性差异,用快速干燥法多核疟原虫与受染红细胞形态变化的检出率分别为1.47%和11.13%;
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  相似匹配句对
     Bloody Mary
     玛丽
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     Method for human peripheral blood eosinophil isolation for patch-clamp study
     适合膜片钳试验的一种外周嗜酸性粒细胞分离法
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     THE METABOLISM OF BLOOD SUGARS OF THE ARMYWORM
     粘虫的糖代谢
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     Design of the chuck of diaphragm
     膜片卡盘的设计
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     2)internal membranous flaps;
     (2)内膜片;
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  blood films
This report concerns a young woman, whose stained blood films revealed a hypochromic and microcytic red cell morphology.
      
Peripheral blood films revealed, only very sparsely, tear drop cells and a few erythroid and myeloid precursors, but no definite leukoerythroblastic reaction.
      
However, vacuolated lymphocytes were found in peripheral blood films and a diagnosis of the juvenile form of NCL (JNCL) was made.
      
Blood films were stained at pH 6 and 6.5, tissue at pH 4.5 and 5.0, both in azure A eosin B.
      
Deamination of alcohol fixed blood films is completed in about 2 min, of sublimate fixed spinal cord in about 1 hr.
      
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A new method, enzyme immunoassay (EIA), for diagnosis of malayan filariasis is described.Of 101 sera from microfilaria carriers tested by the EIA, 97(96. 1%) were positive with 1:40 dilution and 92(91.1%) with 1:80, while of tha 200 healthy persons tested the false-positivity rates were 4% with 1:40 and 1.5% with 1:80. The criterion for positive diagnosis was drawn as follows. Double positives with 1:40 and 1:80 was regarded as positive reactors, and thosepositive with 1:40 alone as doubtful positive reactors....

A new method, enzyme immunoassay (EIA), for diagnosis of malayan filariasis is described.Of 101 sera from microfilaria carriers tested by the EIA, 97(96. 1%) were positive with 1:40 dilution and 92(91.1%) with 1:80, while of tha 200 healthy persons tested the false-positivity rates were 4% with 1:40 and 1.5% with 1:80. The criterion for positive diagnosis was drawn as follows. Double positives with 1:40 and 1:80 was regarded as positive reactors, and thosepositive with 1:40 alone as doubtful positive reactors. By using this criterion a sensitivity of 91.1% and a specificity of 97.5% were obtained.No cross reactions occurred between microfilaria antigen and the sera from the patients with schistosomiasis (20 cases) and paragonimiasis (15 cases). But 1 out of the 10 sera from patients with hookworm disease gave positive cross reaction.Satisfactory reproducibility of the EIA was demonstrated by a comparative experiment, in which 14 known positive and 15 negative sera were tested repeatedly at 10-day's interval.In the field trial, of the 480 subjects living in filariasis endemic areas, both thick film examination and EIA were employed 7 microfilaria carriers were detected by the thick films asthey were also. EIA positive reactors and 20 positive reactors and 24 doubtful positive reactors by the EIA.It is thought that this EIA could be used as a tool for diagnosis of malayan filariasis.

以微丝蚴固相抗原酶免疫试验法,检查了101例微丝蚴血症患者和200例健康人。按本文拟订的标准判断阳性、可疑阳性、假阴性和假阳性率分别为:91.09%,7.92%,0.99%和1.5%。在与血吸虫、肺吸虫和钩虫病人血清作交叉试验中,仅发现与钩虫病人(1/10)有交叉阳性。用本法和厚血膜片法现场检查了480例,发现前者阳性20例,后者7例(其酶免疫试验均阳性)。初步认为,本法诊断丝虫病具有较好的敏感性、特异性和重现性,因而具有一定实用价值。

In order to improve the technique for histochemical demonstration of the different lymphocyte patterns by alpha-naphthyl acetate esterase (ANAE) activity from a single microscopic slide, 10 kinds of comparative experiments were performed. The results showed as follows. The peripheral blood mononuclear cells first fixed in 0.4% glutaldehyde and postfixed in 15% formol calcium gave the best effect on preserving the enzyme activity and on connterstaning of the leucocyte nuclei. During formol calcium fixation, the...

In order to improve the technique for histochemical demonstration of the different lymphocyte patterns by alpha-naphthyl acetate esterase (ANAE) activity from a single microscopic slide, 10 kinds of comparative experiments were performed. The results showed as follows. The peripheral blood mononuclear cells first fixed in 0.4% glutaldehyde and postfixed in 15% formol calcium gave the best effect on preserving the enzyme activity and on connterstaning of the leucocyte nuclei. During formol calcium fixation, the erythrocytes were dissolved from the diluted blood film with PBS more easily than from the blood smear without any dilution. The lymphocytes were distributed more uniformly and densely in the former specimen than in the latter. When incubated with 0.5 mg alpha-naphthyl acetate/ml medium in hexoazotized pararosaniline for 1(1/2)~2 hrs at 37℃, the distinct ANAE patterns of circulating lymphocytes were more readily identified.In 28 healthy adults the differential counts of the lymphocyte ANAE patterns from the diluted blood specimen was similar to those found from the specimen of isolated lymphocytes. The focal pattern was about 56%, the diffuse pattern about 25% and the negative pattern about 19%; while the isolated lymphocyte specimen was compared with the diluted blood film from the same individuals, the focal pattern was decreased insignificantly, the diffuse pattern increased and the negative pattern decreased significantly.

通过对酸性非特异性酯酶(ANAE)标记人周血淋巴细胞方法的10项步骤对比,见到对分离的淋巴细胞以戊二醛弱固定,15%福尔马林钙(1.3%)复固定时,酶活性保存和胞核复染好。此福尔马林钙固定全血膜片,尚具有溶解红细胞的优点,尤其是固定以磷酸缓冲盐水稀释的血膜片,比血涂片更易使片上的红细胞溶去,而使淋巴细胞数目集中,易于计数。以国产重氮化副品红及0.5mg/ml的α-醋酸萘酯浓度的培育液,于37℃培育约2h,ANAE细胞分型辨别较好。共观察28例成人,血片淋巴细胞及分离的淋巴细胞的ANAE分型中,点型约占56%,弥散型约占25%,阴性型约占19%,两者计数结果近似。对同一个体分离的淋巴细胞与血片淋巴细胞做对比观察,见到点型减少不显著,弥散型明显增多,阴性型明显减少。

This paper reports that some factors affected the morphological change of erythrocyte trophozoites of Plasmodium vivax temperate strain in Guizhou Province.The results showed that:(1)The relationship be- tween detection rate of Schuffner's dots and concentration of Giemsa stain solution.The concentration of Giemsa stain solution used varied from 2%,5%,7%,10% to 15%,for the thin blood film,the detec- tion rates of Schuffner's dots in RBC infected with trophozoites were 0.7%,51.9%,69.8%,75.7% and 88.1% respectively,and...

This paper reports that some factors affected the morphological change of erythrocyte trophozoites of Plasmodium vivax temperate strain in Guizhou Province.The results showed that:(1)The relationship be- tween detection rate of Schuffner's dots and concentration of Giemsa stain solution.The concentration of Giemsa stain solution used varied from 2%,5%,7%,10% to 15%,for the thin blood film,the detec- tion rates of Schuffner's dots in RBC infected with trophozoites were 0.7%,51.9%,69.8%,75.7% and 88.1% respectively,and that of RBC ring form were 0%,0.3%,25.4%,18% and 53.8% respective- ly;(2)In the same patient at the different stages of the disease-i.e.chills,fever and intermission stages, the detection rates of Schuffner's dots in RBC infected with trophozoites were 35.4%,56.7% and 88.3% respectively.Multinuclear trophozoites were discovered from the patients infected with plasmodium viuax tem- perate strain,no matter whether they came from malaria areas or malaria free areas.The detection rate of multinuclear trophozoites from the patients of malaria areas was 1.68% and 4.87% from the patients of malaria free areas;(3)The relationship between the method for drying the thin blood film and the detection rate of multinuclear trophozoites(RTM)and of infected erythrocyte morphological change(REMC).When the thin film was dried by the electric fan(fast drying method),the RTM and REMC were 1.47% and 11.13%,and the parasitized red cells were enlarged in 58.99%,only 29.88% the infected erythrocytes appeared normal.When the thin blood films were dried in the moist box(slow drying method),RTM and REMC were found to be 3.30% and 33.71% respectively,the infected erythrocytes enlarged accounted for 52.98%,and only 13.31% appeared normal.

本文报告某些因素对温带株间日疟原虫红内期滋养体形态的影响。结果显示:(1)薛氏点的检出率与使用吉氏(Giemsa)染液的浓度密切相关,分别用2%、5%、7%、10%和15%染色,环状体期被寄生红细胞薛氏点的检出率分别为0%、0.3%、25.4%、18.0%和53.8%,阿米巴期薛氏点的检出率分别为0.7%、51.9%、69.8%、75.7%和88.1%;在同一例病人临床发作的发冷期、发热期和发作间歇期的薄血膜片,以同一浓度的吉氏液染色,薛氏点的检出率分别为35.4%、56.7%和88.3%。(2)来自疟区和无疟区的病人,无论长潜伏期或短潜伏期,均可查获多核疟原虫,来自疟区者检出率为1.68%,来自无疟区者为4.87%。(3)薄血膜片干燥快或慢与多核疟原虫和受染红细胞形态变化的检出率有显著性差异,用快速干燥法多核疟原虫与受染红细胞形态变化的检出率分别为1.47%和11.13%;用延迟干燥法则分别为3.3%和33.71%。

 
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