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blast程序
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  blast program
     The alignment of the sequences of SFB4' and SFB4 by the BLAST program revealed that there is a 4 bp deletion in SFB4' and the 4 bp deletion is TTTA.
     用BLAST程序比较SFB4’基因和SFB4基因时发现:SFB4’基因中有4个碱基的缺失,并且这4个缺失碱基是TTTA。
短句来源
     Mblast: A Multiple Alignment Program Based on Blast Program
     一个基于Blast程序的多重序列对齐程序——Mblast
短句来源
     Method: extract the two worms DNA, design the especial primers using PCRdesn software to PCR, obtain the rRNA ITS2 gene sequence, then compare the gene identical with Blast program of GenBank, compare the gene variation with Primer Premier software, set up the phylogenetic tree using NEIGHBOR programs of PHYLIP software.
     方法:提取融水大囊、小囊肺吸虫成虫的基因组DNA,应用PCRdesn软件设计特异引物对其进行基因体外克隆并测出其rRNA ITS2的基因序列,通过GenBank的Blast程序进行基因的同源性比较,通过Primer Premier软件和PHYLIP软件的NEIGHBOR程序进行基因序列的基因差异比较和基因进化树的构建。
短句来源
     The genomic DNAs of P.skrjabini and P.veocularis metacercariae were extracted,the ITS2 gene in the DNA fragment was amplified by PCR with special primers and the amplified products were sequenced after purification. Meanwhile,the gene sequence homology was analyzed with Blast program of GenBank,and the phylogenetic tree was constructed from the results using ME program of MEGA 3.0 software.
     提取斯氏狸殖吸虫囊蚴和泡囊狸殖吸虫囊蚴的基因组DNA,用特异引物,PCR扩增其ITS2基因并将PCR扩增产物纯化后测序,然后通过GenBank的Blast程序分析基因序列的同源性,并应用MEGA3.0软件中的ME程序构建种系发生树。
短句来源
     In this study, four subjects were investigated:1 Bioinformatic analysis of HER-2/neu RLD proteinThe biological characters of HER-2/neu RLD protein, including antigenic specificity, CTL peptide epitopes, hydrophilic/ hydrophobic nature and isoelectric point, were analysed by Blast program and computer algorithms, such as BIMAS, SYFPEITHI and Goldkey software.
     利用Blast程序、BIMAS、SYFPEITHI和Goldkey等计算机软件对HER-2/neu RLD蛋白的抗原特异性、所包含的CTL表位以及蛋白的亲疏水性、等电点等进行了生物信息学分析。 (二)HER-2/neu RLD2蛋白的可溶性表达及纯化
短句来源
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  “blast程序”译为未确定词的双语例句
     Recombinant was screened and sequenced and analyzed by NCBI/BLAST.
     用NCBI/BLAST程序进行同源性收索。
短句来源
     The results of searching the GenBank by blast methods showed that this sequence and Solanum demissum putative gag-pol polyprotein were 43% homologous in region from 90aa to 243aa.
     用GenBank中的BLAST程序分析表明,该片段与马铃薯(Solanum demissum)反转录转座子的gag-pol聚合蛋白90-243aa区域氨基酸同源性为43%.
短句来源
     DAS and RPS-BLAST procedure were used to predict the structure and function of the hypotheti-cal protein.
     通过DAS及RPS-BLAST程序预测其蛋白质结构及功能。
短句来源
     Similar nucleotide sequences encoding IL-18 of pVAX1-IL-18 were looked up by Blast means in NCBI GenBank.
     利用Blast程序 ,搜索NCBIGenBank中与重组质粒 (pVAX1 IL 18)中编码IL 18基因同源的序列。
短句来源
     The structure and function of the LRP15 protein were predicted through RPS_BLAST software.
     通过RPS_BLAST程序预测其蛋白质结构及功能。
短句来源
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  相似匹配句对
     Sequences were explored in EMBL and NCBI with the Blast and Fastx program.
     并运用Blast和Fastx程序进行检索。
短句来源
     A Disk Copy Program
     磁盘拷贝程序
短句来源
     PROGRAMMED REQUESTS
     程序请求
短句来源
     The structure and function ofthe LRP15 protein were predicted through RPS-BLAST software.
     通过RPS-BLAST程序预测其蛋白质结构及功能。
短句来源
     Parallel Detection Algorithm of V-BLAST
     V-BLAST的并行译码
短句来源
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  blast program
Analysis of the peptide and virus protein sequences with the BLAST program showed that GA has affinity for various surface proteins of several human viruses such as HIV-1, hepatitis C virus, and herpesviruses.
      
Using the BLAST program to compare the sequences against online nucleotide databases, 88% sequences were found in ZmDB or NCBI, and 12% sequences were not found in existing nucleotide databases.
      
Homologous analysis of the ureA against that reported by Clayton CL and the GenBank and SwissProt databases were performed with the BLAST program at the Genome Net through the Internet.
      
Secondly, we made sequence/structure alignments in order to find a template using the BLAST program.
      
The partial amino acid sequence obtained via Edman degradation revealed no significant homology to other reported peptides in the Basic Local Alignment Search Tool (BLAST) program database.
      
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Objective: To investigate the presence of TTV infection among patients with Non A E hepatitis in China and establish the PCR method for detection of TTV DNA. Methods: Serum samples collected from Beijing hospital of infectious diseases were detected for TTV DNA using PCR method and the PCR products were sequenced. Results and Conclusion: TTV DNA was detected in 5 of the 10 patients with Non A E hepatitis.The partial gene was cloned and sequenced from the serum of one patient with non A E hepatitis. The partial...

Objective: To investigate the presence of TTV infection among patients with Non A E hepatitis in China and establish the PCR method for detection of TTV DNA. Methods: Serum samples collected from Beijing hospital of infectious diseases were detected for TTV DNA using PCR method and the PCR products were sequenced. Results and Conclusion: TTV DNA was detected in 5 of the 10 patients with Non A E hepatitis.The partial gene was cloned and sequenced from the serum of one patient with non A E hepatitis. The partial sequence of TTV in China(named as TTVCH1) showed 98% nucleotide identity over the corresponding region of TTV isolate in Japan.

目的:了解我国人群中是否存在TT病毒(TTV)感染,分析国内TT病毒株基因结构特点。方法:用PCR方法检测血清标本中TTVDNA,对TTVDNA阳性的标本进行序列测定。结果:10例临床诊断为非甲至非戊型肝炎病人血清标本中,用PCR方法检测出5例为TTVDNA阳性。将其中的一株命名为TTVCH1(TTV中国株1)并进行序列测定,该序列与日本TTV部分基因(CLON22)相对应位置的核苷酸同源性为98%。用BLAST程序检索了GenBank+EMBL+PDB序列库的332949个序列数据,没有发现与之有明显同源性的序列;与已知的肝炎病毒(A至G)也没有明显的相关性,而只同日本报道的部分基因有很高的同源性。结论:该研究建立了检测TTV的PCR方法并测定了TTV中国株的部分基因序列,首次证实在我国人群中存在TTV的感染。

To rapidly and economically obtain the knowledge about adult stage Schistoma japonicum (Chinese strain) expressed genes by expressed sequence tag( EST) strategy to search for the genes which might result in the identification of new targets for chemotherapy and vaccine development. Methods: A directional cDNA library constructed from Schistosoma japonicum (Chinese strain) adult stage RNA was used to generate expressed sequence tags(ESTs) and the obtained ESTs were compared with EMBL-parasites database and GENBANK...

To rapidly and economically obtain the knowledge about adult stage Schistoma japonicum (Chinese strain) expressed genes by expressed sequence tag( EST) strategy to search for the genes which might result in the identification of new targets for chemotherapy and vaccine development. Methods: A directional cDNA library constructed from Schistosoma japonicum (Chinese strain) adult stage RNA was used to generate expressed sequence tags(ESTs) and the obtained ESTs were compared with EMBL-parasites database and GENBANK datebase by BLASTn and BLASTx. Results : A total 150 phage clones were randomly selected for generating expressed sequence tags (ESTs). From these clones, we obtained 56 EST-quality sequence. Among these EST-quality sequences, 47 ESTs were successfully submitted to the dbEST at GenBank. A total of 7. 1 % of these EST-quality sequences were identified suquence of Schistosoma japonicum , while 3.6% were homologs with homologous sequence of Schistosoma japonicum. A total of 25% of these EST-quality sequences wre sequence of Schistosoma mansoni or other oranisms, and 55.4% of the ESTs which had no matches in the database were classified as unknow sequences. Most ESTs with putative protein identified belonged to functional groups related to the general housekeeping responsibilities. In addition, some information about several interesting genes were found. Conclusion: To get expressed sequence tags(ESTs) by partial cDNA sequencing is a rapid and economical method to accumulate knowledge about the expresset genes of adult stage Schistosoma japonicum ( Chinese strain).

随机挑取日本血吸虫(中国大陆株)成虫cDNA文库单个重组克隆进行部分测序以获得EST(ex-pressed sequence tag),获得的EST通过 BLAST程序同EMBL寄生虫数据库和GeneBank数据库进行比较及同源性分析。结果在随机挑取日本血吸虫(中国大陆株)成虫cDNA文库150个单个重组克隆中,获得了56个有价值的EST序列,其中47个在GeneBank dbEST中登录。7.1%EST序列为日本血吸虫已知序列,3.6%为日本血吸虫同源序列,曼氏血吸虫或其他生物的同源序列占25%,未知序列占55.6%。通过同源性分析可知,大多数同源序列具有看家基因功能。另外,也发现了几个有价值的基因。

The Blast program developed by National Center for Biotechnology Information (NCBI) is one of the powerful tools for sequence analysis including both nucleotide and amino acid sequences. Although it could be used for multiple sequences alignment, the result is not always available. After analysis of the blast result, a new algorithm was designed to optimize the multiple alignment of Blast. A program named "Mblast" was then developed for application. Result demonstrated that the program is useful in identifying...

The Blast program developed by National Center for Biotechnology Information (NCBI) is one of the powerful tools for sequence analysis including both nucleotide and amino acid sequences. Although it could be used for multiple sequences alignment, the result is not always available. After analysis of the blast result, a new algorithm was designed to optimize the multiple alignment of Blast. A program named "Mblast" was then developed for application. Result demonstrated that the program is useful in identifying conserved region of multiple sequences.

核酸序列和蛋白质序列的相似性分析日益成为生物信息学研究的核心内容 .NCBI的Blast程序是进行此类分析的最有力工具 .虽然它提供了初步的将多条序列进行综合对齐的分析方案 ,但是实际效果却很不理想 .在对Blast程序的输出结果进行仔细分析的基础上 ,基于“求同存异”的思想 ,我们编制了一个多重序列对齐程序Mblast.该程序与目前流行的序列多重对齐程序相比 ,更容易检出序列的同源区 .

 
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