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   blast程序 在 基础医学 分类中 的翻译结果: 查询用时:0.513秒
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blast程序
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  blast program
Analysis of the peptide and virus protein sequences with the BLAST program showed that GA has affinity for various surface proteins of several human viruses such as HIV-1, hepatitis C virus, and herpesviruses.
      
Using the BLAST program to compare the sequences against online nucleotide databases, 88% sequences were found in ZmDB or NCBI, and 12% sequences were not found in existing nucleotide databases.
      
Homologous analysis of the ureA against that reported by Clayton CL and the GenBank and SwissProt databases were performed with the BLAST program at the Genome Net through the Internet.
      
Secondly, we made sequence/structure alignments in order to find a template using the BLAST program.
      
The partial amino acid sequence obtained via Edman degradation revealed no significant homology to other reported peptides in the Basic Local Alignment Search Tool (BLAST) program database.
      
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To rapidly and economically obtain the knowledge about adult stage Schistoma japonicum (Chinese strain) expressed genes by expressed sequence tag( EST) strategy to search for the genes which might result in the identification of new targets for chemotherapy and vaccine development. Methods: A directional cDNA library constructed from Schistosoma japonicum (Chinese strain) adult stage RNA was used to generate expressed sequence tags(ESTs) and the obtained ESTs were compared with EMBL-parasites database and GENBANK...

To rapidly and economically obtain the knowledge about adult stage Schistoma japonicum (Chinese strain) expressed genes by expressed sequence tag( EST) strategy to search for the genes which might result in the identification of new targets for chemotherapy and vaccine development. Methods: A directional cDNA library constructed from Schistosoma japonicum (Chinese strain) adult stage RNA was used to generate expressed sequence tags(ESTs) and the obtained ESTs were compared with EMBL-parasites database and GENBANK datebase by BLASTn and BLASTx. Results : A total 150 phage clones were randomly selected for generating expressed sequence tags (ESTs). From these clones, we obtained 56 EST-quality sequence. Among these EST-quality sequences, 47 ESTs were successfully submitted to the dbEST at GenBank. A total of 7. 1 % of these EST-quality sequences were identified suquence of Schistosoma japonicum , while 3.6% were homologs with homologous sequence of Schistosoma japonicum. A total of 25% of these EST-quality sequences wre sequence of Schistosoma mansoni or other oranisms, and 55.4% of the ESTs which had no matches in the database were classified as unknow sequences. Most ESTs with putative protein identified belonged to functional groups related to the general housekeeping responsibilities. In addition, some information about several interesting genes were found. Conclusion: To get expressed sequence tags(ESTs) by partial cDNA sequencing is a rapid and economical method to accumulate knowledge about the expresset genes of adult stage Schistosoma japonicum ( Chinese strain).

随机挑取日本血吸虫(中国大陆株)成虫cDNA文库单个重组克隆进行部分测序以获得EST(ex-pressed sequence tag),获得的EST通过 BLAST程序同EMBL寄生虫数据库和GeneBank数据库进行比较及同源性分析。结果在随机挑取日本血吸虫(中国大陆株)成虫cDNA文库150个单个重组克隆中,获得了56个有价值的EST序列,其中47个在GeneBank dbEST中登录。7.1%EST序列为日本血吸虫已知序列,3.6%为日本血吸虫同源序列,曼氏血吸虫或其他生物的同源序列占25%,未知序列占55.6%。通过同源性分析可知,大多数同源序列具有看家基因功能。另外,也发现了几个有价值的基因。

AIM: To investigate the developmental regulation of β-defensin rBD-2 gene expression in the rat lung. METHOD: Total RNA was isolated from the pulmonary tissues of the fetal, neonatal and adult rats. RT-PCR were performed with primers (P 1: TTCAGTCATGAGGATCCATT AC; P 2: TGGAACTTGGTCTTTTTATCTAC). The RT-PCR products were cloned into pGEM-T easy vector and the recombinant plasmid was analyzed with EcoR1 digestion and the inserted DNA sequencing was performed on ABI PRISM-377 DNA sequencer. RESULTS:...

AIM: To investigate the developmental regulation of β-defensin rBD-2 gene expression in the rat lung. METHOD: Total RNA was isolated from the pulmonary tissues of the fetal, neonatal and adult rats. RT-PCR were performed with primers (P 1: TTCAGTCATGAGGATCCATT AC; P 2: TGGAACTTGGTCTTTTTATCTAC). The RT-PCR products were cloned into pGEM-T easy vector and the recombinant plasmid was analyzed with EcoR1 digestion and the inserted DNA sequencing was performed on ABI PRISM-377 DNA sequencer. RESULTS: Rat β-defensin-2 transcripts were detected in all the pulmonary tissues of rats during different developmental stages, e.g. at just before birth, 8 hours and 4 days after birth , and adult. CONCLUSION: The rat β-defensin-2 is constitutively expressed in the pulmonary tissues, suggesting that β-defensin-2 may play a role in the lung innate defense against infection.

目的 :探讨大鼠 β -防御素 - 2基因在肺组织表达的发育调控。方法 :根据Genbank大鼠 β -防御素- 2的cDNA序列 ,设计一对特异引物。采用RT -PCR技术在大鼠不同发育阶段的肺组织总RNA中 ,扩增其特异cDNA片段 ,并进行DNA序列测定。借助Genbank中BLAST程序 ,将从该cDNA序列推导的氨基酸序列与人的hBD -2和大鼠的rBD - 1做相似性分析。结果 :从足月胎鼠、出生 8h和 4d以及成年大鼠相同重量肺组织所提取的总RNA中 ,均扩增出一条密度相同、长度约为 2 2 0bp的cDNA片段。在所推导氨基酸序列中与hBD - 2氨基酸相同率为48 6 % (17/ 35 ) ,与rBD - 1氨基酸残基的相同率为 31% (11/ 35 )。其成熟分子链长 35个氨基酸残基 ,含 β -防御素共有的且排列次序相同的 6个典型的半胱氨酸和其它保守氨基酸残基。结论 :本实验表明不同发育阶段大鼠肺组织均表达β - 防御素 - 2mRNA ,提示该分子可能是肺组织天然抵抗微生物感染的一个重要分子基础

Aim To rapidly and economically obtain the new genes of adult stage Schistosoma japonicum (Chinese strain) by expressed sequence tag (EST) strategy.Methods A directional cDNA library constructed from Schistosoma japonicum (Chinese strain ) adult stage RNA was used to generate expressed sequence tags(ESTs) and the ESTs obtained were compared against EMBL-parasites database and GenBank datebase by BLASTn and BLASTx.Results A total 200 phage clones were randomly selected for generating expressed sequence tags(ESTs).From...

Aim To rapidly and economically obtain the new genes of adult stage Schistosoma japonicum (Chinese strain) by expressed sequence tag (EST) strategy.Methods A directional cDNA library constructed from Schistosoma japonicum (Chinese strain ) adult stage RNA was used to generate expressed sequence tags(ESTs) and the ESTs obtained were compared against EMBL-parasites database and GenBank datebase by BLASTn and BLASTx.Results A total 200 phage clones were randomly selected for generating expressed sequence tags(ESTs).From these clones,we obtained 76 EST-quality sequence.A total of 7.9% of these EST-quality sequences were identified suquence of Schistosoma japonicum,while 5.3% were putatively identified sequence of Schistosoma japonicum.A total of 22.4% of these EST-quality sequences were putatively identified sequence of Schistosoma mansoni or other organisms,and 59.2% which had no matches in the database were classified as unknow sequence.Among these EST-quality sequences,66 ESTs were successfully submitted to the dbEST at GenBank.In addition,some interesting genes were found.Conclusion Partial cDNA sequencing to get expressed sequence tags(ESTs) was a rapid and economical method to discover new genes of adult stage Schistosoma japonicum(Chinese strain).

目的 运用表达序列标签 (ExpressedSequenceTag ,EST)技术快速、经济地发现日本血吸虫 (中国大陆株 )新基因。方法 随机挑取日本血吸虫 (中国大陆株 )成虫cDNA文库单个重组克隆进行部分测序以获得EST ,获得的EST通过BLAST程序同EMBL寄生虫数据库和GenBank数据库进行比较及同源性分析。结果 本研究共随机挑取日本血吸虫 (中国大陆株 )成虫cDNA文库单个重组克隆 2 0 0个 ,获得了 76个有EST价值的序列 ,其中 7.9%为日本血吸虫已知序列 ,5 .3 %为日本血吸虫同源序列。曼氏血吸虫或其他生物的同源序列占有EST价值的序列的 2 2 .4 % ,未知序列占 5 9.2 %。在获得的76个有EST价值的序列中 ,66个成功在GenBankdbEST中登录。通过同源性分析 ,发现了一些令人感兴趣的基因。结论 EST方法有助于快速、经济地发现日本血吸虫 (中国大陆株 )成虫新基因。

 
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