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严重急性呼吸
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  severe acute respiratory
     Severe acute respiratory syndrome (SARS) is a newly emerged infectious disease caused by SARS-associated coronavirus (SARS-CoV).
     严重急性呼吸综合症(Severe acute respiratory syndrome ,SARS)是一种新型传染性疾病,其病原体为SARS病毒(S ARS-associated coronavirus ,SARS-CoV)。
短句来源
     Severe acute respiratory syndrome(SARS) is an emerging severe acute respiratory system infectious disease which is caused by SARS coronavirus(SARS-CoV).
     严重急性呼吸综合征(severe acute respiratory syndrom e,SARS)是由SARS冠状病毒(SARS coronavirus,SARS-CoV)感染引起的一种新发严重急性呼吸系统传染病。
短句来源
     Severe acute respiratory syndrome (SARS) is a newly emerged human infectious disease caused by the severe acute respiratory syndrome coronavirus (SARS-CoV).
     严重急性呼吸综合症(Severe acute respiratory syndrome, SARS)是由SARS冠状病毒(SARS-CoV)引起的人类新发传染病,S、M、N、E蛋白是SARS-CoV病毒粒子的几种主要结构蛋白。
短句来源
     SARS-CoV is a newly discovered coronavirus which was identified as the etiological agent of severe acute respiratory syndrome (SARS).
     SARS冠状病毒(SARS-CoV)为一种新发现的冠状病毒,可导致人的严重急性呼吸综合征(severe acute respiratory syndrome,SARS)。
短句来源
     Meta analysis on T cell subsets of patients with severe acute respiratory syndrome
     严重急性呼吸综合征T细胞亚群Meta分析
短句来源
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  critical severe acute respiratory
     The analysis of clinical features and warning indicators in patients with critical severe acute respiratory syndrome
     重症严重急性呼吸综合征临床特点及其预警指标的分析
短句来源
     Objective: To summarize the experience of treatment in 32 critical severe acute respiratory syndrome(SARS) patients.
     目的 :总结 32例重症严重急性呼吸综合征 (SARS)患者的救治经验。
短句来源
     Objective To evaluate the efficacy of nasal continuous positive airway pressure ventilation (n - CPAP) in the treatment of critical severe acute respiratory syndrome( SARS) .
     目的 探讨鼻罩持续气道正压通气(n-CPAP)治疗严重急性呼吸综合征(SARS)的疗效。
短句来源
     The objective was to analyze the pneumothorax and mediastinal emphysema occurred after the non-invasive and invasive mechanical ventilation(MV)and to discuss relevant strategy in respiratory treatment in patients with critical severe acute respiratory syndrome(SARS).
     回顾性分析31例行机械通气的严重急性呼吸综合征(SARS)危重病例,189例未行机械通气的SARS病例和近期外科ICU机械通气治疗的89例急性呼吸窘迫综合征(ARDS)病例,以分析危重SARS患者行无创或有创机械通气后气胸及纵隔气肿的发生情况,并探讨相应的呼吸治疗策略。 结果31例行机械通气的SARS危重患者中9例出现气胸及纵隔气肿;
短句来源
     To analyze tracheal intubation and respiratory treatment in the critical severe acute respiratory syndrome (SARS)patients.
     目的对13例重症严重急性呼吸综合征(severeacuterespiratorysyndrome,SARS)患者的气管插管及呼吸治疗进行分析。
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  “严重急性呼吸”译为未确定词的双语例句
     Expression and Application of SARS Coronavirus N Gene
     严重急性呼吸综合征(SARS)冠状病毒N蛋白基因的表达及其初步应用
短句来源
     Serological methods (the Indirect ELISA and the Double antigen sandwich ELISA) to detect the specific antibody in the serum of SARS patients were compared.
     对血清学方法(间接ELISA法和双抗原夹心ELISA法)检测严重急性呼吸综合征(SARS)患者特异性抗体的效果进行了比较。
短句来源
     Clinical Analysis of 61 Patients with SARS
     61例严重急性呼吸综合征临床分析
短句来源
     Clinical analysis for 33 cases of SABS
     严重急性呼吸综合征(SARS)33例临床分析
短句来源
     Changes of Neutrophil CD11b in Patients with SARS and Its Clinical Significance
     严重急性呼吸综合征患者中性粒细胞CD11b的变化及意义
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  severe acute respiratory
The aim of this research was to investigate the blood coagulation function in the patients with avascular necrosis of the femoral head (ANFH) after severe acute respiratory syndrome (SARS).
      
Expression of prothrombinase/fibroleukin gene fg12 in lung impairment in a murine severe acute respiratory syndrome model
      
To evaluate the role of murine fibrinogen like protein 2 (mfgl2) /fibroleukin in lung impairment in Severe acute respiratory syndrome (SARS), a murine SARS model induced by Murine hepatitis virus strain 3 (MHV-3) through trachea was established.
      
In addition, new emerging infections like severe acute respiratory syndrome (SARS) and epidemic influenza also have to be taken into account.
      
Large artery ischaemic stroke in severe acute respiratory syndrome (SARS)
      
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  critical severe acute respiratory
Short term outcome and risk factors for mortality in adults with critical severe acute respiratory syndrome (SARS)
      
The independent risk factors to predict mortality of critical severe acute respiratory syndrome (SARS) were investigated.
      


The nucleocapsid protein sequence of the SARS coronavirus was firstly identified at the protein level.The nucleocapsid protein of the SARS coronavirus was purified and identified by using proteomics technology.The LC MS/MS result showed that the molecular weight of the N protein of the SARS coronavirus ranges between 47kD and 52kD,the coverage of the amino acid sequence is more than eighty-seven percent between the actual and the predicted nucleocapsid...

The nucleocapsid protein sequence of the SARS coronavirus was firstly identified at the protein level.The nucleocapsid protein of the SARS coronavirus was purified and identified by using proteomics technology.The LC MS/MS result showed that the molecular weight of the N protein of the SARS coronavirus ranges between 47kD and 52kD,the coverage of the amino acid sequence is more than eighty-seven percent between the actual and the predicted nucleocapsid protein of the SARS coronavirus.

利用蛋白质组学技术,对纯化的严重急性呼吸综合征(SARS)冠状病毒颗粒所含核蛋白进行初步分离与鉴定。质谱分析结果最终表明,SARS冠状病毒核蛋白的分子量位于47kD与52kD之间,所获得的SARS冠状病毒核蛋白的质谱分析数据覆盖了所预测病毒核蛋白氨基酸序列的87%,且符合率为100%。从而首次从蛋白质水平对SARS冠状病毒核蛋白的氨基酸序列进行了证实。

The sev ere acute respiratory syndrome(SAR S) has been becoming a worldwide i nfectious disease,since emerged in Guangdong Province of China in Nov ember 2002.Using ten kinds of cell lines,virus isolation was carried out with 3 samples of autopsy lung tissue,2 samples of autopsy spleen ,1 early-stage blood sample and 2 throat swabs from SARS patients.Al l the samples tested were previousl y screened by RT-PCR for SARS coro navirus,showing to contain SARS co ronavirus specific nucleotide sequ ences.After...

The sev ere acute respiratory syndrome(SAR S) has been becoming a worldwide i nfectious disease,since emerged in Guangdong Province of China in Nov ember 2002.Using ten kinds of cell lines,virus isolation was carried out with 3 samples of autopsy lung tissue,2 samples of autopsy spleen ,1 early-stage blood sample and 2 throat swabs from SARS patients.Al l the samples tested were previousl y screened by RT-PCR for SARS coro navirus,showing to contain SARS co ronavirus specific nucleotide sequ ences.After inoculating onto monola yer of cell lines with these sampl es,cytopathogenic effects(CPE)appea red in the cell lines 293,RD,Vero, Vero-E6 and HeLa.Although the abil ities of various samples to induce CPE in different cell lines were d istinct,the incubation period of C PE as well as the morphological ch aracteristics of CPE in these cell lines were not significantly diffe rent.Indirect immunofluorescence a ssays of infected cells with sera from convalescent patients , RT-PCR of RNAs extracted from the infected cells with SARS coron avirus specific primers identified t hat the isolated viruses from vari ous specimens of SARS patients,col lected both at acute stage and by autopsy,were SARS coronavirus. This confirms again that the novel cor onavirus isolated is the causal agent for S ARS,which distributes in various t issues and organs during infection course in vivo and is capable of i nfecting manifold cell lines in vi tro. Isolation of SARS virus from se rum strongly implies that there ma y be viremia during the pathogenes is of SARS. Key words:coranavirus; severe acute respir atory syndrome (SARS);samples;virus isolation

严重急性呼吸综合征(SARS)自2002年11月在中国广东爆发后,已迅速蔓延成为全球性传染疾患。为了了解SARS冠状病毒的特征,对先前SARS冠状病毒PCR检测呈阳性的来自广东的3份尸检肺组织标本、2份尸检脾组织标本,来自北京的2份咽拭子标本和1份血清标本,利用10种不同的细胞系分离病毒。结果显示,上述标本在感染细胞后,分别可在293、Vero-E6、Vero、RD和HeLa细胞系中产生细胞病变(CPE)。不同标本在上述细胞系中致CPE的能力不同,但CPE出现的时间和病变形态学特征无显著性差异。以恢复期SARS病人血清为抗体,用间接免疫荧光法对感染后细胞培养的检测,冠状病毒RT-PCR对感染后细胞RNA的检测,初步证明分离的病毒为冠状病毒。结果再次证明冠状病毒为SARS的病原,它具有较广泛的器官分布和细胞感染能力。血清中SARS冠状病毒的分离,高度提示在SARS发病过程中存在有病毒血症。

To investigate the pathog en that caused the epidemic of severe acute respirat ory syndrome (SARS )in Guangzhou region,the clinical specimens including oropharyng eal washings and autopsy specimens were collected and inoculated onto cell line monolayer, including human embryonic lung, MDCK, Hep-2 and chic ken embro for virus isolation. Pati ents'serum IgG antibody was als o detected by indirect immunofluor escence assay(IFA)during the conva lescent phase.The isolated pathoge n was further detected by nested...

To investigate the pathog en that caused the epidemic of severe acute respirat ory syndrome (SARS )in Guangzhou region,the clinical specimens including oropharyng eal washings and autopsy specimens were collected and inoculated onto cell line monolayer, including human embryonic lung, MDCK, Hep-2 and chic ken embro for virus isolation. Pati ents'serum IgG antibody was als o detected by indirect immunofluor escence assay(IFA)during the conva lescent phase.The isolated pathoge n was further detected by nested r everse transcription-polymerase ch ain reaction(RT-PCR)and immunoelec tron microscopy.Thirteen pathogens were successfully isolated in huma n embryonic lung and Hep-2 cell fr om 75 oropharyngeal washings and 3 autopsy specimens,and a specific s equence of 110bp product was obtai ned by the nested RT-PCR amplifica tion procedure.The product was con firmed as coronavirus by DNA seque ncing.The prepared coronavirus ant igen was used to test thirty SARS patients'blood specimens during co nvalescent phase.Twenty six among thirty blood specimens showed IgG antibody positive,by the same time ,all thirty control sera(from common feverish patients)were all nega tive for SARS IgG antibody.By immuno electron microscopy,coronavirus an d chlamydial particles both were f ound in autopsy lung tissue combin ed with SARS patient's convalescen t serum.Therefore,pathogen isolate d from tissue culture is believed to be responsible for SARS.The seq uencing data of the isolated patho gen confirms that the virus is cor onavirus.

为查找引起广州地区流行的严重急性呼吸综合征(SARS)的病原体,采集患者漱口液及尸解标本,用组织培养法接种人胚肺细胞、MDCK细胞、Hep-2细胞和鸡胚分离病毒,用间接免疫荧光法检测患者恢复期血清IgG抗体,确定分离的病原是SARS的主要病因,再用套式RT-PCR、免疫电镜法鉴定病原。结果用人胚肺、Hep-2细胞在75份漱口液和3例尸解组织中分离出13株病原体,经套式RT-PCR扩增出110bp的特异产物,经测序证实为冠状病毒。制备冠状病毒的抗原,检测30份SARS病人恢复期血,其中26份血清IgG抗体阳性。同时检测30份普通发热病人血清作对照,IgG抗体全部阴性。由此证明,经组织培养分离到的病原体是引起SARS的致病因子,用分子生物学方法测序后证实为冠状病毒。

 
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