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系膜细胞培养
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  mesangial cells were cultivated
     MethodsMesangial cells were cultivated with various concentrations of LPS, while the synthetic secretion of IL-12 in supernatants of these cells was detected by ELISA techniques.
     方法应用不同浓度的脂多糖对系膜细胞培养 ,采用ELISA法检测培养细胞上清液IL 12的分泌。
短句来源
     To investigate the effect of interleukin-13 (IL-13) on interleukin-12 (IL-12) production in mesangial cells,mesangial cells were cultivated with LPS (10 μg/ml) or/and various concentration of IL-13 and the protein synthesis of IL-12 in mesangial cells was measured by ELISA.
     为了探讨白细胞介素 13(IL 13)对体外培养的人肾小球系膜细胞产生白细胞介素 12 (IL 12 )的影响。 我们用脂多糖(LPS 10 μg/ml) ,不同浓度的IL 13对系膜细胞培养 ,分别采用ELISA法和半定量RT PCR法检测细胞上清液的IL 12和系膜细胞IL 12p4 0mRNA表达。
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  “系膜细胞培养”译为未确定词的双语例句
     5. After 24 hours of ET-1(10-7mol/L) incubation, the level of LN in culture supernatant of MCs was significantly higher than that of control (P<0.01).
     5. ET-1(10-7mol/L)作用24小时,系膜细胞培养上清LN的浓度较对照组显著升高(P<0.01)。
短句来源
     CTGF increased the p42/44MAPK phosphorylation. Pretreatment of the cells with PD98059 or UO126, inhibitors of phosphorylated p42/44 MAKP, decreased the CTGF-induced expression of phosphorylated p42/44 MAPK and concentration of FLK in supernatants.
     CTGF可诱导p42/p44MAPK磷酸化,PD98059或UO126可抑制这一作用,并部分抑制CTGF诱导的系膜细胞培养上清液中FLK的分泌。
短句来源
     2. Cultured human embryonic mesangial cells(MC) were exposed to ET-1 , the expressions of MMP-3 and TIMP-1 proteins in MCs were examined by cell ELISA.
     2. 采用体外人系膜细胞培养,应用细胞ELISA法检测ET-1对系膜细胞表达MMP-3及TIMP-1的影响。
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     Methods: Got SD rats kidney for culture of fibroblasts, added the rats serum with different concentration of BST to fibroblasts, added the human serum from healthy volunteers with diHerents concentration of BST to mesangial cells.
     方法:取SD大鼠肾脏作成纤维细胞体外培养,以保肾片喂服SD大鼠,取不同浓度含药大鼠血清加入体外培养的成纤维细胞中。 以健康志愿者服用保肾片,取不同浓度含药人血清加入系膜细胞培养体系中。
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     Results SOD and GSH? PX were lowered,while MDA and O 2 -· increased in the supernatant of high glucose culture without TPs.
     结果 :高糖可降低系膜细胞培养上清液中SOD、GSH PX的活力 ,增加MDA和O2 -· 的含量 ;
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     Methods Human glomerular mesangial cells were conventionally cultured.
     方法人类系膜细胞常规培养
短句来源
     STIMULATION OF MESANGIAL CELLPROLIFERATION BY METHYLPREDNISONE
     甲基强的松龙刺激培养系膜细胞增殖
短句来源
     Primary culture studies have shown that exogenous NGF induces differentiation of NB cells depending their survival on NGF.
     一、细胞培养
短句来源
     1. Cell culture
     一、细胞培养:
短句来源
     Methods Mesangial cells were cultured in RPMI-1640 medium containing 10% newborn calf serum.
     方法(1)原代培养大鼠系膜细胞;
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In this paper. the effects of serum from 38 patients with IgA nephropathy on cultured gloumerular mesangial cells in vitro were studied. the IgA-CIC, IgG-CIC and IgM-CIC in the serum were measured by using ELISA. The results were summarized as follows: 1. the ~3H-TdR uptake into mesangial cells was markedly enhanced (P<0.01) by the serum of IgA nephropathy on culture medium, and the increment was closely related to the severity of glomerular pathological changes (r=0.429; 2. the levels of IgA-CIC and IgG-CIC...

In this paper. the effects of serum from 38 patients with IgA nephropathy on cultured gloumerular mesangial cells in vitro were studied. the IgA-CIC, IgG-CIC and IgM-CIC in the serum were measured by using ELISA. The results were summarized as follows: 1. the ~3H-TdR uptake into mesangial cells was markedly enhanced (P<0.01) by the serum of IgA nephropathy on culture medium, and the increment was closely related to the severity of glomerular pathological changes (r=0.429; 2. the levels of IgA-CIC and IgG-CIC increased in the serum of patients with IgA nephropathy than those in normal controls (P<0.05); and 3. there was a close relationship between the amount of urinary protein and the degree of renal pathological changes (r=0.616).

本研究采用系膜细胞培养技术观察了38例IgA肾病患者血清对系膜细胞增殖的影响,并采用ELlSA方法检测了血清中循环免疫复合物(CIC)的浓度,结果发现,IgA肾病血清对系膜细胞的增殖有明显刺激作用,而且这种刺激作用与肾组织病理改变程度成正相关,IgA肾病血清中IgA-CIC和IgG-CIC均较正常人明显增高,但血中IgA水平与系膜增殖及病理改变程度无相关性。

In this paper, the method of cultured rat mesangial ceils (Ms ceils) and morphologic analysis of Ms cells with DMPS membrane were firstly established. The effect of Cyclosporine A (CsA)on the Ms cells contraction and the protection of Ms cells from CsA toxicity using platelet-activating factor (PAF) antagonist-BN52021 were observed. In results, CsA induced Ms cells contraction in time and concentration dependent manner. CsA (10~(-9)M) also had the effect and lasted half an hour. Twenty minutes after removing...

In this paper, the method of cultured rat mesangial ceils (Ms ceils) and morphologic analysis of Ms cells with DMPS membrane were firstly established. The effect of Cyclosporine A (CsA)on the Ms cells contraction and the protection of Ms cells from CsA toxicity using platelet-activating factor (PAF) antagonist-BN52021 were observed. In results, CsA induced Ms cells contraction in time and concentration dependent manner. CsA (10~(-9)M) also had the effect and lasted half an hour. Twenty minutes after removing of CsA from cells, the Ms cells could recover from their contracting states. Ms cells contraction induced by CsA was significantly inhibited by PN52021, at 5×10~(-5)M. The results indicate: 1) The effect of CsA induced Ms cells contraction is reversible and the contraction of Ms cells can reduce renal blood ilow and glomerular capillary kf in rive. 2) PAF may take part in the cell contraction in some way. PAF antagonist BN52021 has the effect on prophylaxis and treatment of CsA nephrotoxicity.

本文采用SD大鼠肾脏系膜细胞培养及硅胶膜形态分析技术。研究环孢素A(CsA)诱导肾系膜细胞收缩作用及血小板活化因子拮抗剂BN52021对CsA诱导作用的抑制效应。结果示CsA引起系膜细胞收缩呈现时间和剂量依赖效应,其作用可逆。BN52021浓度为5×10~(-5)M时,可显著地抑制CsA的诱导作用。结果提示CsA通过可逆性诱导系膜细胞收缩引起肾小球毛细血管血流量和超滤系数(Kf)下降;血小板活化因子可能参与了这个过程,使用血小板活化因子拮抗剂可能有预防及治疗CsA肾毒性的作用。

Transforming growth factor β (TGF β) levels in the supernatant of glomeruli and mesangial cells conditioned culture media were measured withan standard bioa-ssay system. The results showed that both glomeruli and mesangial cells were capable of producing certain amount of TGF β. Glomeruli only liberated latent form of TGF β, while mesangial cells produced about 10% active form of TGF β. Considering the unique role of TGF β in regulating cell growth and extracellular matrix metabolism, TGF β secreted by glomeruli...

Transforming growth factor β (TGF β) levels in the supernatant of glomeruli and mesangial cells conditioned culture media were measured withan standard bioa-ssay system. The results showed that both glomeruli and mesangial cells were capable of producing certain amount of TGF β. Glomeruli only liberated latent form of TGF β, while mesangial cells produced about 10% active form of TGF β. Considering the unique role of TGF β in regulating cell growth and extracellular matrix metabolism, TGF β secreted by glomeruli and mesangial cells might be important in maintaining the normal glomerular structure and function.

用标准的生物学方法检测了肾小球和系膜细胞培养上清中转化生长因子(TGF_s)的水平。结果显示:肾小球和系膜细胞均可产生一定量的TGF_s;肾小球产生的THF_s无活性,而系膜细胞产生的TGF_s大约10%有活性。鉴于TGF_s在调节细胞生长和细胞外基质代谢中的独特作用,肾小球和系膜细胞来源的rGF_s可能在维持正常肾小球结构和功能中起重要作用。

 
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