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肾细胞株
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  kidney cell line
     Methods The human mononuclear cell line (U 937 ), human T lymph cell line (Molt 4), human gull cell line (Kirlich), swine kidney cell line (PK15), rat testicle cell line (STE), human liver cancer cell line (HepG2 and Hep3B) were infected with positive serum of HCV RNA, and the infection and replication of HCV RNA in those cell lines were studied by RT PCR.
     方法 选用 HCV RNA阳性血清感染人单核细胞株 (U937)、人 T淋巴细胞株 (Molt- 4)、人胆囊细胞株 (Kirlich)、猪肾细胞株 (PK15 )、鼠睾丸细胞株 (STE)、人肝癌细胞株 (Hep G2和 Hep3B)等 7种细胞。 应用 RT- PCR法检测 HCV RNA在这 7种细胞中的感染和复制情况。
短句来源
     orale,M. hyorhinis and M. arginini,which had chronically infected the mouse myeloma Sp2/0 and African green monkey kidney cell line Vero were erdicated under such treatment. After mycoplasmas decontamination,Sp2/0 retained the usual rate of fusion and the ability of producing monoclonal antibodies.
     人为用口腔支原体、猪鼻支原体、精氨酸支原体分别感染小鼠骨髓瘤Sp2/0和非洲绿猴肾细胞株Vero,然后经MC—210处理,支原体全部去除,且不影响Sp2/0在制备杂交瘤时的融合率和抗体的分泌。
短句来源
     The encoding sequence of human brain acetylcholinesterase was subcloned into an eukaryotic expression vector pcDNA 3.1 and then transfected into human embryonic kidney cell line 293 for expression of recombinant human acetylcholinesterase.
     人脑乙酰胆碱酯酶的全长cDNA序列克隆到真核高效表达载体pcDNA3.1中 ,并将pcDNA AChE转染人胚肾细胞株 2 93细胞 ,进行rhAChE的暂时表达 .
短句来源
     Methods: The shRNA expression vector targeting HBV core antigen gene was designed and constructed. Human embryonic kidney cell line AD293 was co-transfected with HBcAg-EGFP fusion protein expression vector and shRNA expression vector transiently, and the cells without shRNA-transfection and with non-specific shRNA transfection were used as controls.
     方法设计并构建针对HBcAg基因的小发夹RNA表达载体,将构建好的shRNA表达载体和HBcAg-增强型绿荧光蛋白融合蛋白表达载体共转染人胚肾细胞株AD293,以空载体组以及针对无关序列的shRNA表达载体组为阴性对照,流式细胞术和实时荧光定量PCR法检测RNAi的抑制效果。
短句来源
     METHODS: MTT assay was used to detect cytotoxic ities of AP to normal human liver cell line Chang Liver (C. Liver), normal human embryo kidney cell line 293, and normal human umbilicus vein endothelial cell l ine ECV304. The 3 cell lines were treated with AP before or after irradiation.
     方法:采用MTT法检测AP对人正常肝细胞株ChangLiver、人正常胚肾细胞株293和人正常脐静脉内皮细胞株ECV304的细胞毒作用。
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  kidney cell lines
     Expression of PKD1 and PKD2 transcripts and proteins and its significance in different types of kidney tissues and kidney cell lines
     多囊肾病基因1和多囊肾病基因2在不同肾组织和肾细胞株中的表达及意义
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     BHK and primary rabbit kidney cell lines were used to study the anti_HSV_Ⅱ effect of 8 kinds of water extracts of phyllanthus plants in vitro.
     为探讨叶下珠属植物对单纯疱疹病毒Ⅱ型的抑制作用 ,采用地鼠肾细胞株 (BHK)和原代兔肾细胞对 8种叶下珠属植物水提物进行了体外抗单纯疱疹病毒Ⅱ型作用的研究。
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     Objective To prepare and identify monoclonal antibody against LRR-WSC domain of polycystin-1 and to investigate the distribution of polycystin-1 in kidney tissues and kidney cell lines.
     目的 用杂交瘤技术制备抗多囊蛋白-1 LRR-WSC区单克隆抗体,检测多囊蛋白-1在肾组织和肾细胞株中的分布和定位。
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  “肾细胞株”译为未确定词的双语例句
     Materials and methodsAdenoviral vectors: Ad/GT-TRAIL , Ad/GT-LacZ , Ad/GT-Bax and Ad/PGK-GV16. Human embryonal kidney cells transformed by introducing sheared fragments of Ad5 DNA, 293cell line, human colon cancer cell line RKO and drug doxorubicin.
     腺病毒载体Ad/GT-TRAIL,Ad/GT-LacZ,Ad/GT-Bax及Ad/PGK-GV_(16),分别携带人TRAIL、BAX和细菌LacZ基因。 阿霉素,人胚肾细胞株293,人大肠癌细胞株 RKO。
短句来源
     Molecular Cloning of Highly Reiterated Sequence AGMr (HindⅢ)-1in Vero Cell Line of African Green Monkey and Its Preliminary Application
     非洲绿猴肾细胞株AGMr(HindⅢ)-1高度重复顺序的克隆及初步应用
短句来源
     METHODS Whole cell patch clamp technique was used to record changes of Kv4 2 and Kv4 3 potassium currents.
     方法 采用全细胞膜片钳技术记录稳定表达Kv4 2和Kv4 3电流的人胚胎肾细胞株 (HEK2 93细胞 )电流的变化。
短句来源
     The expression and analysis of its activity of anti-bacterial peptide gloverin in COS-7 cells
     抗菌肽葛佬素在非洲绿猴肾细胞株COS-7中的表达及其活性的初步分析
短句来源
     Methods: D116H ANG pAxCAwt cosmid DNA and DNA TPC were mixed and co transfected to the 293 cell by calcium phosphate coprecipitation.
     方法 :ANG衍生物 D116 H - ANG重组粘粒与腺病毒 DNA末端肽复合物混合后以磷酸钙共沉淀法转染 2 93人胚肾细胞株细胞。
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  kidney cell line
Soluble Tankyrase Located in Cytosol of Human Embryonic Kidney Cell Line 293
      
In embryonic kidney cell line 293 the enzyme was excluded from the nuclei and distributed in fractions of soluble cytosolic proteins and low-density microsomes.
      
Recombinant NP was synthesized in Escherichia coliand in human kidney cell line 293 cotransfected with recombinant vaccinia virus vTF7-3 expressing T7 RNA polymerase.
      
The genes that were induced and suppressed in human embryonic kidney cell line RH upon the infection with tick-borne encephalitis virus were studied by the method of subtractive hybridization.
      
Their cotransfection into the human embryonic kidney cell line HEK293T provided the production of a full-size recombinant human antibody.
      
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  kidney cell lines
Numerical and structural karyotypic variability was investigated in the NBL-3-17 and NBL-3-11 "markerless" rat kangaroo kidney cell lines cultivated on a fibronectin-coated surface.
      
A cell-surface antigen(s) coded for by the chromosome 7 common to all human fibroblastic cell lines tested and also found on African green monkey kidney cell lines was demonstrated.
      
Normal fibroblasts and normal kidney cell lines reacted negatively to mA33.
      
The chromosome complement of two ovine (Ovis aries L.) kidney cell lines are described.
      
High-resolution replication banding patterns were induced in prometaphase and prophase chromosomes of Xenopus laevis by treating kidney cell lines with 5-bromodeoxyuridine (BrdU) and deoxythymidine (dT) in succession.
      
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This article reports the the morphogenesis of multinuclear giant cell. The cell came of giant cell in human renal cell culture. Its process was forming several new nucleoli by the nucleolus of giant cell with budding manner, The cells were cultured for 24 hours. The invaginated nuclear membrane surround ed the nucleoli. Finally the connecting part of the nuclear membrane was br-oken. Several cell nuclei were formed within the cytoplasm, thus forming the multigiant cell. The fusion of cels was not to be found....

This article reports the the morphogenesis of multinuclear giant cell. The cell came of giant cell in human renal cell culture. Its process was forming several new nucleoli by the nucleolus of giant cell with budding manner, The cells were cultured for 24 hours. The invaginated nuclear membrane surround ed the nucleoli. Finally the connecting part of the nuclear membrane was br-oken. Several cell nuclei were formed within the cytoplasm, thus forming the multigiant cell. The fusion of cels was not to be found.

本丈主要研究多核巨细胞的发生,采用人肾细胞株培养24小时,观察发现其中的多核巨细胞来源于巨细胞,其过程是核仁以出芽方式形成若干个新核仁,核膜内陷,包围核仁,最后核膜连接部断裂,在细胞质内形成若干个细胞核,即形成多核巨细胞,未见细胞融合。

A virus isolated from hemorrhagic grass carp at Shao Yang County, Hunan Provice revealed icosahedral particles of approximately 71 nm in diameter with a double capsid under eletron microscopic observations. Polyacrylamide gel eletrophoresis showed that the viral genome was composed of 11 segments of dsRNA. This was proved by RNase or DNase treatment and acridine orange staining as well as nucleic acid denaturing assay. The virus replicated well in the CIK cell line, forming plaqus approximately 2 mm in diameter...

A virus isolated from hemorrhagic grass carp at Shao Yang County, Hunan Provice revealed icosahedral particles of approximately 71 nm in diameter with a double capsid under eletron microscopic observations. Polyacrylamide gel eletrophoresis showed that the viral genome was composed of 11 segments of dsRNA. This was proved by RNase or DNase treatment and acridine orange staining as well as nucleic acid denaturing assay. The virus replicated well in the CIK cell line, forming plaqus approximately 2 mm in diameter in monolayer culture. It was ether and chloroform resistant, stable at pH 3.7 and 10, and at a temperature of 56℃ for 30 min. This virus is distinct from any of the Min Jiang, Chang Jiang, Yellow River, and may be a novel member of this family.

从湖南邵阳地区患出血病的草鱼组织中分离到一株病毒,经部分提纯、负染后,电镜观察,揭示为双层衣壳、直径约71nm的球形颗粒。其核酸在聚丙烯酰胺凝胶电泳中呈现11条带。经核酸酶消化、吖啶橙染色及核酸的变性试验,证明为双链RNA。病毒在草鱼肾细胞株(CIK)单层细胞上,形成直径约2mm的空斑;对温度有一定稳定性;对乙醚、氯仿不敏感;经pH3、10处理后仍能保持相对稳定的滴价。此病毒不属业已建立的呼肠孤病毒科6个属中的任何一个属,而可能为呼肠孤病毒科中一新成员。

Liver emulsion of rabbits which died of rabbit haemorrhagic disease ( RHD ) was inoculated onto DJRK cells, a continuous cell line established from rabbit kidney cells. After 3 passages, the CPE was rather regular, most of the cells became round,aggregated and finally detached. Im munofluorescence was mainly found in the cytoplasm around the nucleus by indirect immunofluorescent assay.The 5th passage cell culture virus was inoculated into 8 susceptible rabbits. Viral HA was detected in all serum samples and...

Liver emulsion of rabbits which died of rabbit haemorrhagic disease ( RHD ) was inoculated onto DJRK cells, a continuous cell line established from rabbit kidney cells. After 3 passages, the CPE was rather regular, most of the cells became round,aggregated and finally detached. Im munofluorescence was mainly found in the cytoplasm around the nucleus by indirect immunofluorescent assay.The 5th passage cell culture virus was inoculated into 8 susceptible rabbits. Viral HA was detected in all serum samples and lasted for several days. All rabbits died at day 5-11 postinoculation. The pathological changes were typical of RHD. Similar results were obtained with the 10th and 16th passage materials.It was shown by electronmicroscopy that there were numerous virions in the cells infected with the virus of 10th passage, 30-34nm in diameter, mainly accumulated around the nuclei, and some in the nuclei.The 11th passage cell culture virus inactivated with formaldehyde was inoculated into healthy rabbits. Very high HI antibody was produced and the rabbits could resist challenge with viurlent RHDV.

用自建的乳兔肾细胞株(DJRK)培养兔出血症病毒,第3代后开始出现规律性细胞病变。于接种后24~72小时,可见大量细胞变圆、聚集和脱落,部分细胞拉丝,呈不规则形态。间接免疫荧光检查表明,感染早期呈核内荧光,而后胞浆内亦出现荧光,尤以核周围明显。 用第5代细胞培养物接种8只易感兔,2天后血清中出现2~(?)~2~(12)血凝滴度,持续数天。剖检死兔,其病变与兔出血症典型病变相符,肾病变尤为显著。用第10、16代毒接种易感兔,结果相似。 在第10代感染细胞的超簿切片中,见到大量直径约30~34nm的病毒颗粒,主要分布于胞核周围和胞浆内,核内亦可见散在的病毒颗粒。 将第11代细胞培养物用甲醛灭活后接种易感兔,2周后HI效价升高达2~(10),攻毒全部获得保护,证明细胞培养病毒具有良好的免疫原性。

 
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