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染色质结构
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  chromatin structure
    The effect of overtraining on human sperm chromatin structure
    过度训练对人精子染色质结构的影响
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    3.Application flow cytometry to analysis sperm chromatin structure and analysing the results with the sofeware of Denovo corporationAThe expert detect the work and life enviroment of the soldiers and record the maximam value .
    3、应用流式细胞仪进行精子染色质结构分析,用Denovo software公司软件(DNS)分析结果。
短句来源
    The Semen Dectection and Sperm Chromatin Structure Assay
    空军雷达作业人员精液检测与精子染色质结构分析
短句来源
    Methods: Molecular epidemiological investigation in some tank soldiers was carried out by questionnaire, physical examination and advanced lab analyses such as automatic semen analysis system to analyze semen quality, single cell gel electrophoresis and flowcytometer to detect sperm DNA damage and chromatin structure.
    方法 :通过填写问卷调查表、体检及采用全自动精液分析系统分析精液质量、单细胞凝胶电泳和流式细胞仪检测DNA单双链损伤以及分析染色质结构等先进实验检测方法对某部装甲兵进行分子流行病学调查。
短句来源
    Comet assay and sperm chromatin structure assay showed no significant change.
    彗星实验和染色质结构分析则无明显变化。
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  “染色质结构”译为未确定词的双语例句
    TopoisomeraseⅠ,Ⅱ and poly(ADP-ribose) polymerase are key enzymes in regulation of chromosome structure and play important roles in DNA replication,RNA transcription and DNA damage repair.
    拓朴异构酶Ⅰ和Ⅱ、聚腺苷二磷酸核糖聚合酶等是参与调节染色质结构的关键酶,在DNA复制、转录及损伤修复等重要的生命事件中起着十分重要的作用。
短句来源
    Flow cytometry provides a unique ,rapid,and efficient means to measure effects of reproductive toxins and potential mutagens and is thus a useful tool for epidemiological studies.
    精子染色质结构分析(SCSA)是一种独特的、快速的、检测生殖毒素和潜在致突变剂的影响的有效方法、是流行病学研究的有力工具。
短句来源
    Objective To investigate the genetic damage to testicular germ cells and sperms of mice induced by inhalation of Depleted Uranium(DU) aerosol.
    目的 初步探讨小鼠吸入不同剂量的贫铀 (DU)气溶胶对生精细胞和精子的遗传损伤作用。 方法 采用小鼠睾丸初级精母细胞染色体畸变试验、小鼠睾丸生殖细胞微核试验和精子染色质结构试验 (SCSA) ,评价DU诱导小鼠生精细胞和精子的遗传损伤程度。
短句来源
    Conclusion Overtraining could induce sperm DNA injury and affect sperm activity,thus to decrease the potentiality of reproduction.
    结论 过度训练后可导致精子染色质结构的变化 ,影响精子活力 ,使生育潜能降低。
短句来源
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  chromatin structure
The role of small double-stranded RNAs is considered in formation of silent chromatin structure.
      
Also we describe a number of known methyl-DNA-binding proteins and links between methylation of DNA and higher-order chromatin structure.
      
The functional role of the CpNpG-hypermethylation of satellite DNA is probably associated with formation of a specialized chromatin structure simultaneously regulating expression of a large number of genes in the cells of M.
      
The chromatin structure in these regulatory regions is important to chromosome organization for accurate regulation of nuclear processes.
      
Three-Dimensional Reconstruction of the Chromatin Structure in the Somatic Nucleus of the Infusorian Bursaria truncatella
      
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TopoisomeraseⅠ,Ⅱ and poly(ADP-ribose) polymerase are key enzymes in regulation of chromosome structure and play important roles in DNA replication,RNA transcription and DNA damage repair.We used an orthogonal design to analyze the role of topoisonoraseⅠ,Ⅱ and poly(ADP-ribose) polymerase in the regulation of chromosome structure and the production of DNA damages after irradiation.It was observed that their inhibitors could loose chromosome structure and increase DNA damages in irradiated cells,and they interacted...

TopoisomeraseⅠ,Ⅱ and poly(ADP-ribose) polymerase are key enzymes in regulation of chromosome structure and play important roles in DNA replication,RNA transcription and DNA damage repair.We used an orthogonal design to analyze the role of topoisonoraseⅠ,Ⅱ and poly(ADP-ribose) polymerase in the regulation of chromosome structure and the production of DNA damages after irradiation.It was observed that their inhibitors could loose chromosome structure and increase DNA damages in irradiated cells,and they interacted with each other.This result shows that these three enzymes facilitate the integrity of chromosome structure and promote radioresistant ability of cells.The interaction between these three enymes reflects their internal relation.

拓朴异构酶Ⅰ和Ⅱ、聚腺苷二磷酸核糖聚合酶等是参与调节染色质结构的关键酶,在DNA复制、转录及损伤修复等重要的生命事件中起着十分重要的作用。本实验采用正交设计方法分析它们在细胞染色质调控方面的功能以及对辐射所致DNA损伤的影响。结果表明,经过相应的酶抑制剂作用后,染色质结构的松弛程度增加,受照后DNA的初始损伤加重,且三种酶之间存在交互作用,提示出这些酶可能在使染色质结构紧密化及提高辐射抗性方面起有利作用;所观察到的交互作用可能反映了三种酶之间的联系。

Objective To investigate the genetic damage to testicular germ cells and sperms of mice induced by inhalation of Depleted Uranium(DU) aerosol. Methods Genetic damage due to DU to spermatogenic cells and sperms of mice was assessed by chromosome aberration test, micronucleus test and sperm chromatin structure assay(SCSA) of the primary spermatocytes of mice. Results No difference in micronucleus rate of testicular germ cells was found between DU aerosol group and the control group The chromosome numerical...

Objective To investigate the genetic damage to testicular germ cells and sperms of mice induced by inhalation of Depleted Uranium(DU) aerosol. Methods Genetic damage due to DU to spermatogenic cells and sperms of mice was assessed by chromosome aberration test, micronucleus test and sperm chromatin structure assay(SCSA) of the primary spermatocytes of mice. Results No difference in micronucleus rate of testicular germ cells was found between DU aerosol group and the control group The chromosome numerical aberration rates of the groups inhaling DU aerosol at the doses of (731 52±35 99) ng/g and (877 83±43 19) ng/g were 17 2% and 27 2%, respectively, increasing significantly as compared with that of the control group( P <0 01) SCSA revealed that the average α T values, %COMP values and %GREEN values in DU aerosol groups increased significantly than those in the control groups( P <0 05) Conclusion Inhalation of DU aerosol may result in genetic damage to testicular germ cells and sperms of mice

目的 初步探讨小鼠吸入不同剂量的贫铀 (DU)气溶胶对生精细胞和精子的遗传损伤作用。方法 采用小鼠睾丸初级精母细胞染色体畸变试验、小鼠睾丸生殖细胞微核试验和精子染色质结构试验 (SCSA) ,评价DU诱导小鼠生精细胞和精子的遗传损伤程度。结果 吸入DU气溶胶组睾丸生殖细胞微核发生率与对照组相比无显著差异 ,吸入 ( 73 1 5 2± 3 5 99)和 ( 877 83± 43 19)ng gDU组小鼠睾丸初级精母细胞染色体数目畸变率为 17 2 %和 2 7 2 %,与对照组比较显著升高 (P <0 0 1) ;精子染色质结构试验中 ,对照组、吸入 ( 73 1 5 2± 3 5 99)和 ( 877 83± 43 19)ng gDU组的精子SCSA各变量平均值 :αT分别为 2 5 3 5 1± 3 1 76、2 88 2 9± 68 3 0、2 94 5 6± 72 90 ,%COMP分别为 3 73± 5 5 0、9 0 2± 4 43、9 11± 5 3 3 ,%GREEN分别为 0 89± 0 44、1 62± 1 0 6、1 80± 0 61,D...

目的 初步探讨小鼠吸入不同剂量的贫铀 (DU)气溶胶对生精细胞和精子的遗传损伤作用。方法 采用小鼠睾丸初级精母细胞染色体畸变试验、小鼠睾丸生殖细胞微核试验和精子染色质结构试验 (SCSA) ,评价DU诱导小鼠生精细胞和精子的遗传损伤程度。结果 吸入DU气溶胶组睾丸生殖细胞微核发生率与对照组相比无显著差异 ,吸入 ( 73 1 5 2± 3 5 99)和 ( 877 83± 43 19)ng gDU组小鼠睾丸初级精母细胞染色体数目畸变率为 17 2 %和 2 7 2 %,与对照组比较显著升高 (P <0 0 1) ;精子染色质结构试验中 ,对照组、吸入 ( 73 1 5 2± 3 5 99)和 ( 877 83± 43 19)ng gDU组的精子SCSA各变量平均值 :αT分别为 2 5 3 5 1± 3 1 76、2 88 2 9± 68 3 0、2 94 5 6± 72 90 ,%COMP分别为 3 73± 5 5 0、9 0 2± 4 43、9 11± 5 3 3 ,%GREEN分别为 0 89± 0 44、1 62± 1 0 6、1 80± 0 61,DU组各变量值与对照组比较均有显著增高 (P <0 0 5 )。结论 小鼠吸入贫铀气溶胶可诱导生精细胞和精子遗传损伤

Objective:To explore the effect of physical factors such as noise,vibration quality in tank soldiers during training, so as to provide basis for making up intervention measures to reduce and prevent male infertility. Methods: Molecular epidemiological investigation in some tank soldiers was carried out by questionnaire, physical examination and advanced lab analyses such as automatic semen analysis system to analyze semen quality, single cell gel electrophoresis and flowcytometer to detect sperm DNA damage...

Objective:To explore the effect of physical factors such as noise,vibration quality in tank soldiers during training, so as to provide basis for making up intervention measures to reduce and prevent male infertility. Methods: Molecular epidemiological investigation in some tank soldiers was carried out by questionnaire, physical examination and advanced lab analyses such as automatic semen analysis system to analyze semen quality, single cell gel electrophoresis and flowcytometer to detect sperm DNA damage and chromatin structure. Results: The sperm survival rate and normal sperm ratio in exposed group〔(62.30±25.24)% and (81.28±11.18)% respecitvely〕 were lower than those in control group〔(68.02±25.72)%,(93.56±5.03)% respectively, P<0.05,P<0.01〕,while sperm abberation rate in exposed group (19.06±11.15)% was higher than that in control group 〔(7.37±10.60)%,P<0.01〕. However, all these data were in normal range. The sperm vitality in exposed group(36.4%) was significantly lower than that in control(43.5%,P<0.01) and normal value(>50%). Comet assay and sperm chromatin structure assay showed no significant change. Conclusion: Long term exposure to noise and vibraton may induce decrease in semen quality but not DNA damage in sperm. It is necessary to improve individual protection and to make up preventive measures for male reproductive care in tank soldiers.

目的 :探讨装甲兵训练噪声振动等物理因素是否对男性精液质量产生影响 ,为制定干预措施 ,降低和预防男性不育的发生提供依据。方法 :通过填写问卷调查表、体检及采用全自动精液分析系统分析精液质量、单细胞凝胶电泳和流式细胞仪检测DNA单双链损伤以及分析染色质结构等先进实验检测方法对某部装甲兵进行分子流行病学调查。结果 :暴露组精液中精子存活率 (6 2 .30± 2 5 .2 4 ) %和正常精子比率 (81.2 8± 11.18) %较对照组 (6 8.0 2± 2 5 .72 ) %和 (93.5 6± 5 .0 3) %下降 (P <0 .0 5 ,P <0 .0 1) ,精子畸形率则明显地增高〔(9.0 6± 11.15 ) %vs (7.37±10 .6 1) % ,P <0 .0 1〕 ,但均在正常值范围内 ,仅精子活力 (36 .4 % )明显低于正常值 (>5 0 % )和对照组 (43.5 % ,P <0 .0 1)。彗星实验和染色质结构分析则无明显变化。结论 :长期受到噪声振动的影响 ,可能发生精液质量下降 ,但未发现精子DNA损伤增多。提示 ,加强装甲兵的个人防护 ,制...

目的 :探讨装甲兵训练噪声振动等物理因素是否对男性精液质量产生影响 ,为制定干预措施 ,降低和预防男性不育的发生提供依据。方法 :通过填写问卷调查表、体检及采用全自动精液分析系统分析精液质量、单细胞凝胶电泳和流式细胞仪检测DNA单双链损伤以及分析染色质结构等先进实验检测方法对某部装甲兵进行分子流行病学调查。结果 :暴露组精液中精子存活率 (6 2 .30± 2 5 .2 4 ) %和正常精子比率 (81.2 8± 11.18) %较对照组 (6 8.0 2± 2 5 .72 ) %和 (93.5 6± 5 .0 3) %下降 (P <0 .0 5 ,P <0 .0 1) ,精子畸形率则明显地增高〔(9.0 6± 11.15 ) %vs (7.37±10 .6 1) % ,P <0 .0 1〕 ,但均在正常值范围内 ,仅精子活力 (36 .4 % )明显低于正常值 (>5 0 % )和对照组 (43.5 % ,P <0 .0 1)。彗星实验和染色质结构分析则无明显变化。结论 :长期受到噪声振动的影响 ,可能发生精液质量下降 ,但未发现精子DNA损伤增多。提示 ,加强装甲兵的个人防护 ,制定预防保健措施对提高男性生殖健康保健十分必要。

 
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