Objective To establish anti anti idiotypic antibody (anti anti id, Ab 3 ) of monoclonal anti idiotypic antibody (anti id, Ab 2 ) NP30 of Schistosoma japonicum.
Objective To study the effects of the monoclonal anti idiotypic antibody NP30 active immunization on egg granuloma formation and hepatic fibrosis in Schistosoma japonicum infection.
A “sandwich” ELISA for epitopes of plasminogen(Pg) in serum Lipoprotein(a) Lp(a) ,using monoclonal anti apo(a) as the capture antibody and quantitating with anti Pg enzyme conjuate had been developed.
By using the hybridoma technique,fivehybridoma cell lines secreting monoclonal antibodies (MAbs) against Mhpproteins, which were designed respectively as 11C6, 11B6-1, 14C11-1, 10C11-2 and 12E7-1,were established.
Results Three hybridoma cell strains were obtained and named EGF-B 2, EGF-C 7, and EGF-A 8, which produced monoclonal antibodies specifically against rhEGF. The isotypes of the monoclonal antibodies were IgG 1κ, IgG 1λ, and IgG 3κ and the affinity constant were 2.76×10 10, 3.2×10 9, and 1.45×10 9, respectively.
As soluble antigen, in IRMA it reacted with anti-BSM antibodies used both as a catcher and a tracer, as well as in combination with monoclonal anti-CA19-9 antibodies as a catcher.
A pair of monoclonal anti-CBP70 antibodies were selected; this permitted us to use a sandwich-type immunoenzyme analysis in combination with the streptovidin-biotin system, and this improved substantially the sensitivity of the CBP70 measurement.
Furthermore, the analgesic effect of IFN-α is reversible by monoclonal anti-IL-2 antibody.In vitro experiments show that IFN-α significantly increases the production of IL-2 in a dose dependent manner.
After successful fusion of spleen cells immunized with polymerized human serum albuminreceptor(PHSA-R) activity positive HBV particles, with myeloma cells (sp2/0), a stable monoclonalantibody (McAb) cell line was established. The McAb was identified to be specific for the PreS2antigen of HBV. Since it can inhibit the PHSA-R activity on HBV, it may probably be an antibodyagainst PHSA-R of PreS2. This Mc-anti-PreS cell line was the first one to be reported in ourcountry. with this McAb, we developed a hemaggl...
Anti-idiotype antibodies (Ab2) weresuccessfully induced by monoclonal anti-HBsantibodies (Ab1) in syngenic BALB/c mice.They(Ab2)were identified by RPHA, ELISAand BASA tests. The results showed thatthe Ab2 were able to combine not onlywith monoclonal anti-HBs from mouse butalso with polyclonal anti-HBs antibodiesfrom horse.It was suggested that a commonantigenic determinant existed both in mo-noclonal anti-HBs from mouse and in poly-clonal anti-HBs antiserum from horse. Asmonoclonal antibodies can only induc...
A series of hybridoma cell lines producing monoclonal antibodies to DNA had been obtained by hybridization of spleen cells from MRL-1Pr/1pr mice, which had developed a severe form of SLE and spontaneously produced high levels of antibodies to DNA without exogenus immunization, with SP_2/0-Ag-14 plasmacytomas. The McAb H241, one of those monoclonal antibodies, was used to immunize NZW rabbit and an anti-H241 idiotypic antibody was harvested from immune serum of the rabbit successfully. The sensitivity of H24...