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家蚕bmn细胞
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  bombyx mori n cells
     Transient expression of human growth hormone genomic DNA in Bombyx mori N cells
     人生长激素基因组DNA在家蚕BmN细胞中的瞬时表达
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  “家蚕bmn细胞”译为未确定词的双语例句
     The IC50 value of BmN and Sf9 cells to 2,4-DCP is about 0.14 mmol·L~-1, to NP about 0.05 mmol·L~-1. If the concentration of NP exceeded 0.128 mmol·L~-1, it can induce the DNA of BmN breakage, nevertheless, it also have concentration effect.
     2种细胞对2,4-DCP的IC_(50)约为0.14 mmol·L~(-1),对NP约为0.05 mmol·L~(-1)。 0.128mmol·L~(-1)以上浓度的NP,能诱导家蚕BmN细胞DNA链发生断裂,导致DNA损伤,而且具有较明显的浓度效应。
短句来源
     Observation on the Inoculation and Propagation of the Nosema bombycis in BmN Cell
     家蚕微孢子虫(Nosema bombycis)向家蚕BmN细胞接种与增殖的观察
短句来源
     the cgh gene was cloned into baculovirus transfer vector pBacPAK-His, and homology recombinant with linear baculovirus Bm-BacPAK-6 in Bombyx mori BmN cell, the recombinant viruses were screened by plaque assay.
     将cgh基因克隆进杆状病毒转移载体pBacPAK-His,在家蚕BmN细胞中,与线性化杆状病毒Bm-BacPAK-6重组,通过空斑筛选,获得重组病毒。
短句来源
     Screening under G418 at 800 μg/mL final concentration for three months a stable transformation cell strain with over 80% fluorescent cells was obtained.
     以该载体转染家蚕BmN细胞,用终浓度800μg/mL的遗传霉素(geneticin,G418)筛选3个月,获得了稳定转化的细胞,呈现绿色荧光的细胞数达80%以上。
短句来源
     NP can incur the break of DNA strands of BmN cellsas a result of the damage when the concentration over 0.0128 mmol/L.
     当NP 的浓度超过0.128mmol/L时,能诱导家蚕BmN 细胞DNA 链发生断裂,导致DNA 损伤。
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  相似匹配句对
     Transient expression of human growth hormone genomic DNA in Bombyx mori N cells
     人生长激素基因组DNA在家蚕BmN细胞中的瞬时表达
短句来源
     Study on Apoptosis of BmN Cells Induced by CdCl_2
     CdCl_2诱导家蚕细胞(BmN)凋亡的研究
短句来源
     The Infective Characterics of Beet Armyworm(Spodoptera exigua) Nucleopolyhedrovirus Infect the Silkworm(Bombyx mori)Cell Line BmN
     甜菜夜蛾核型多角体病毒(SeNPV)对家蚕细胞株(BmN)的感染
短句来源
     Effects of Environmental Hormone Atrazine on the Cultured BraN Cells
     环境激素阿特拉津对家蚕卵巢培养细胞(BmN)凋亡的影响
短句来源
     Inhibition of 35℃ on the Development and Propagation of the Nosema bombycis in BmN Cells
     35℃高温对家蚕微孢子虫在BmN细胞中发育、增殖的抑制
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utured

在1000ml滚瓶中,用微载体Cytodex3培养家蚕细胞,在合适的培养条件下(细胞接种浓度3.6×105细胞/ml、微载体浓度5g/L),5天后,细胞的终密度达2.8×106细胞/ml,细胞增长指数为7.9。在细胞指数生长期(48~60小时)用载有人α-干扰素(IFN-α)基因的重组病毒接种(感染复数为0.4PFU/细胞),5天后,培养上清中IFN-α效价为6.4×106IU/ml,是用方瓶培养的家蚕BmN细胞IFN-α的表达量的4倍。

Distribution of silkworm BmN cell attachment to Cytodex 3 microcarrier was observed and could be described by Poisson distribution equation. From both empirical results and by application of the poisson equation the proportion of empty beads was shown. Influence of cell inoculation concentration and microcarrier concentration on cell growth was studied here. The critical cell number was determined to be 6. 4 per micro-carrier bead. The minimal cell inoculation concentration was 1.0×105/ml when Cytodex 3 microcarrier...

Distribution of silkworm BmN cell attachment to Cytodex 3 microcarrier was observed and could be described by Poisson distribution equation. From both empirical results and by application of the poisson equation the proportion of empty beads was shown. Influence of cell inoculation concentration and microcarrier concentration on cell growth was studied here. The critical cell number was determined to be 6. 4 per micro-carrier bead. The minimal cell inoculation concentration was 1.0×105/ml when Cytodex 3 microcarrier concentration was 3g/L.

观察了家蚕BmN(从Silkworm Bombyx mori获得的细胞系)细胞在微载体Cytodex3上的贴壁分布,提出其分布符合Poisson规律,并由此估计了不同细胞接种浓度时裸球的百分比,与实际观测结果基本符合;研究了不同接种浓度与微载体浓度时细胞的生长情况,家蚕BmN细胞在Cytodex3上生长的临界接种数为一珠粒6.4个细胞。当微载体浓度为3g/L时,最低的接种浓度为1.0×10~5/ml。

A HBeAg gene fragment, which has some restriction endonucleased sites on both 5′ ends, including pre c signal peptide sequence and 5′ 447 bp of the HBcAg gene was amplified by PCR. The HBeAg gene fragment was cloned into the BmNPV transfer vector pBm030 and the chimeric vector pBmHBe was constructed. The BmN cells were co transfected with pBmHBe and Wt BmNPV DNA,therefore, the recombinant viruses were obtained by plaque purification. Analysis of the HBeAg antigenecity by ELISA showed that the highest titer...

A HBeAg gene fragment, which has some restriction endonucleased sites on both 5′ ends, including pre c signal peptide sequence and 5′ 447 bp of the HBcAg gene was amplified by PCR. The HBeAg gene fragment was cloned into the BmNPV transfer vector pBm030 and the chimeric vector pBmHBe was constructed. The BmN cells were co transfected with pBmHBe and Wt BmNPV DNA,therefore, the recombinant viruses were obtained by plaque purification. Analysis of the HBeAg antigenecity by ELISA showed that the highest titer in the cell cultural medium was up to a dilution of 1∶32000. Although HBeAg protein also presents in the BmN cells the titer was only 1:2000. The HBcAg protein was fewer than HBeAg (<1∶160) whatever in culture medium and in cells. the results showed that the BmN cells can recognize the HBeAg signal peptide sequence and cut it correctly for HBeAg. The BmN PV BmN cell system is considered to be much better than E.coli system for producing HBeAg protein.

以PCR技术扩增含有PreC信号肽序列及完整的HBeAg基因的序列(即HBcAg基因5′端447bp),在5′端加上合适的酶切位点,克隆到家蚕核多角体病毒转移载体pBm030上,与野生型BmNPVDNA共转染家蚕BmN细胞,空斑纯化后得到多角体基因失活的重组病毒。ELISA法测定表明培养液上清中HBeAg效价达1∶32000,细胞内HBeAg效价为1∶2000,培养液及细胞内的HBcAg含量极低(<1∶160)。研究结果表明,BmN细胞能正确识别与切割HBeAg信号肽序列,所表达的HBeAg效价高,纯度好,明显优于大肠杆菌表达系统

 
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