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荧光原位
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  fluorescence in situ
     Methods:Ki-67 labeling index(Ki-67LI), fluorescence in situ hybridization(FISH), and quantitative PCR(LightCyclerTMPCR) were used.
     方法Ki67标记染色(Ki67LI)、荧光原位杂交(FISH)和定量PCR法(LightCyclerTMPCR)被用于研究。
短句来源
     Objective To explore the value of dual-color fluorescence in situ hybridization(D-FISH) in the detection of inv(16) in acute myeloid leukemia (AML).
     目的 探讨双色荧光原位杂交 (dual- color fluorescence in situ hybridization,D- FISH)对检测急性髓系白血病 (acute myeloid leukemia,AML )中 inv(16 ) (p13q2 2 )异常的价值。
短句来源
     Methods SpectrumOrange~(TM) labeled sequence-specific DNA probe D13S319 for 13q14 and fluorescence in situ hybridization(FISH) were used to detect del(13q14) in 37 patients with MM.
     方法运用SpectrumOrangeTM标记的位于13q14的序列特异性DNA探针D13S319和荧光原位杂交(FISH)技术对37例MM患者的细胞进行染色体13q14的检测。
短句来源
     Methods Spectrum Orange~ TM labeled sequence-specific DNA probe D13S3 19 for 13q14 and interphase fluorescence in situ hybridization (I-FISH) were applied to detect del(13q14) in 83 patients with B-CLL.
     方法运用Spectrum OrangeTM标记的位于13q14的序列特异性DNA探针D13S319和间期荧光原位杂交(I-FISH)技术对83例初发的B细胞CLL(B-CLL)患者的细胞进行染色体13q14的检测。
短句来源
     In order to investigate the immunophenotypic characteristics of bone marrow cells in AML-M2 patients with AML-ETO gene rearrangement classified by FAB, immunophenotype of bone marrow cells in 17 AML-M2 patients with AML-1/ETO+confirmed by fluorescence in situ hybridization was analyzed by using flow cytometry as compared with immunophenotype in 34 APL patients with AML-1/ETO-.
     应用流式细胞术分析17例经荧光原位杂交(FISH)检测AML-1/ETO融合基因阳性、FAB分型为AML-M2(M2/ETO+)患者骨髓的免疫表型,并与34例AML-1/ETO阴性、FAB分型AML-M3、临床诊断为APL患者进行了比较。
短句来源
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  fluorescent in situ
     Identification of 1B/1R Translocations in Wheat Background by Fluorescent in Situ Hybridization
     用荧光原位杂交检测小麦背景中的1B/1R易位
短句来源
     Then determinated MyT1mRNA-positive cells in cortex of epileptic SD rats by fluorescent in situ hybridization with the dig-labeled oligonucleotides.
     采用5′末端标记地高辛的寡核苷酸探针荧光原位核酸分子杂交检测癫性发作后早期大鼠大脑皮质MyT1 mRNA阳性细胞数量。
短句来源
     Objective To set up a fluorescent in situ hybridization(FISH) based method to detect the gene-deleted female carriers of Duchenne/Becker muscular dystrophy (DMD/BMD).
     目的 建立应用荧光原位杂交 (fluorescent in situ hybridization,FISH)方法检查进行性假肥大性肌营养不良 (Duchenne/ Becker muscular dystrophy,DMD/ BMD)患者家系中女性亲属是否为携带者的方法。
短句来源
     FISH (Fluorescent in situ Hybridization) further revealed that the binding sites for the primer set NOR R1 were only on nucleolar organizing region of chromosome 1R.
     荧光原位杂交进一步显示,NORR1引物的结合位点仅位于1R染色体上核仁组织区。
短句来源
     ASSIGNMENT OF A NOVEL ZINC FINGER GENE ZNF191 TO HUMAN CHROMOSOME 18Q12.1 BY HUMAN/RODENT SOMATIC CELL HYBIRD PANEL AND FLUORESCENT IN SITU HYBRIDIZATION
     用人/啮齿类体细胞杂种系及荧光原位杂交将人类新基因ZNF191定位于染色体18q12.1
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  fluorescence in-situ
     The analysis on the sperms X,Y and chromosome 18 by fluorescence in-situ hybridization of patients with infertility,
     不育症患者精子X,Y及18染色体的荧光原位杂交分析
短句来源
     Segregation of Sex Chromosomes in the Spermatozoa of 46,XY/47,XXY Patients with Oligozoospermia by Dual Fluorescence in-situ Hybridization
     双色荧光原位杂交检测46,XY/47,XXY少精子症患者精子性染色体
短句来源
     Objective:To analysis on the sperms X, Y and chromosome 18 by the fluorescence in-situ hybridization of patients with infertility.
     目的:分析不育症患者精子X,Y,18染色体的荧光原位杂交情况。
短句来源
     4. Abnormality of chromosome 21 in embryos of hypoevolutism and embryos with >40% fragment were analysed by fluorescence in-situ hybridization using chromosome 21 locus specific identifier probe (LSI21);
     4.将发育迟缓组和胚胎碎片40%组的胚胎,用21号染色体特殊位点探针(LSI21,SpectrumOrange)探针进行荧光原位杂交,观察两组胚胎21染色体异常情况;
短句来源
     The morphological features were observed by Wright's staining,inverted microscope and electron microscope. CD_(80),CD_(86),CD_(83),CD_(1a)and HLA-DR expression were assayed by flow cytometry,cytogenetic analysis was performed by fluorescence in-situ hybridization(FISH). The concentration of IL-12 was measured by ELISA and the function of antigen presenting was tested by mixed lymphocyte reaction(MLR).
     形态学(Wright染色、倒置显微镜、透射电镜),免疫学(CD80、CD86、CD83、CD1a、HLA-DR)鉴定,荧光原位杂交(FISH)进行细胞遗传学分析,ELISA检测分泌IL-12的能力和混合淋巴细胞反应(MLR)检测抗原递呈功能。
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  “荧光原位”译为未确定词的双语例句
     Fluorescense in -situ hybridization (FISH) was used to confirm the karyotype 46,XX,t(1;5;12)(1pter→1q23::12q22→12qter;5qter→5p11::1q25→1qter;12pter→12q22::1q23→1q25::5p11→5pter).
     经荧光原位杂交(FISH)技术检测,证实患者的核型为46,XX,t(1;5;12)(1pter→1q23::12q22→12qter;5qter→5p11::1q25→1qter;12pter→12q22::1q23→1q25::5p11→5pter).
短句来源
     Method:DNA Fiber-FISH and interphase FISH were both used to detect translocation genes associated with AML-M_2. 50 interphase nuclei were observed.
     方法用DNA纤维荧光原位杂交(DNA Fiber-FISH)技术检测M2型急性髓细胞性白血病(AML-M2)相关易位基因,观察50个间期核荧光信号,并与间期核FISH比较。
短句来源
     Fiber FISH results indicated that the longest string of beads was 6.55 μm,while the shortest one was 1.82 μm long,which were equal to 16.44 and 4.56 kb correspondingly based on a stretching factor of 2.51 kb/μm.
     伸展DNA纤维荧光原位杂交结果显示,端粒最长的线状信号长度为6.55μm,最短的为1.82μm,依据2.51kb/μm的标准,它们分别相当于16.44kb和4.56kb。
短句来源
     The abnormal signals were screened by interphase-fluorescence in situ hybridization (FISH) with dual-color break-apart 11q23/MLL- specific probe,and the 11q23/MLL gene rearrangements were determined by metaphase-FISH.
     LSI MLL 双色分离信号 DNA探针行间期荧光原位杂交(FISH)筛选异常信号,有异常信号者行中期 FISH 确定 11q23/MLL 基因重排。
短句来源
     In contrast,probe pSc20H.
     荧光原位杂交结果显示pSc20H.
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  fluorescence in situ
The karyotype analysis and physical locations of 45S rDNA were carried out by means of fluorescence in situ hybridization in three species, and two forms of Sophora, two species of Robina, and one species of Amorpha.
      
Also, fluorescence in situ hybridization (FISH) was conducted to detect nuclear derivation of the embryos.
      
based on rDNA and Cot-1 DNA fluorescence in situ hybridization
      
Cot-1 DNA contains rDNA and chromosome sites identity between Cot-1 DNA and 25S rDNA was determined by dual-colour fluorescence in situ hybridization.
      
Fluorescence In Situ Hybridization in Studying the Human Genome
      
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  fluorescent in situ
The Detection of Cyanobacterial Cells by a Non-Fluorescent in situ Hybridization Method
      
The results were validated by using fluorescent in situ hybridization (FISH) analysis with centromere-specific DNA probes.
      
Fluorescent in situ hybridization (FISH) was used to locate the satellite DNA on chromosomes and on extended DNA of A.
      
Using fluorescent in situ hybridization (FISH), three human BAC clones, localized in the terminal region of human chromosome 17p (HSA17p13; 1.44-3.68 Mp), were mapped in chromosome 8p of American mink (MVI8p).
      
Lupin kernel fiber consumption modifies fecal microbiota in healthy men as determined by rRNA gene fluorescent in situ hybridiza
      
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  fluorescence in-situ
Cytological evidence from pairing studies, C-banding, and fluorescence in-situ hybridization, showed that both M17 and W66 are wheat/rye multi-addition lines with rye chromosome constitutions of 1R+6R, and 1R+4R, respectively.
      
were identified by the fluorescence in-situ hybridization (FISH) method.
      
KAI-1 has been mapped to the p11.2 region of human chromosome 11 by fluorescence in-situ hybridization analysis.
      
Background: We have recently explored the detection of circulatory male fetal cells directly in maternal whole blood samples by fluorescence in-situ hybridization (FISH).
      
Fluorescence in-situ hybridization revealed that the repopulating hepatocytes arise from cell fusion between transplanted monkey EB cells and recipient mouse hepatocytes.
      
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In this work we used two ehloroplast mutants,the stripe leaves and albino leaves as experimental materials to study the variation of chlorophyll-protein complexes(CPC) in maize.Results obtained indicate that there are five bands of CPC after SDS-polyacrylamide gel electropheresis:CPI,LHCP1,LHCP2,LHCP3,CPa and the band of free chlorophyll(FC).In stripe leaf mutant the height of three peaks of LHOP1,LHCP2,and LHCP3 decreases in comparison with the normal plants of the standard,of them the LHCPi peak decreases...

In this work we used two ehloroplast mutants,the stripe leaves and albino leaves as experimental materials to study the variation of chlorophyll-protein complexes(CPC) in maize.Results obtained indicate that there are five bands of CPC after SDS-polyacrylamide gel electropheresis:CPI,LHCP1,LHCP2,LHCP3,CPa and the band of free chlorophyll(FC).In stripe leaf mutant the height of three peaks of LHOP1,LHCP2,and LHCP3 decreases in comparison with the normal plants of the standard,of them the LHCPi peak decreases more significantly.In the mutant of albino leaves with some green tissue the LHCP1 peak becomes very low and the LHCP2,LHCP3 peaks disappear completely.In order to test the absorption spectra after electrophoresis the gel columns were scaned under 675 nm and 650 nm of the densitometer.Curves obtained show that the mutant of pure albino leaves does not show any LHCP peak under 650 nm.This means that the synthesis of light-harvesting chlorophyll a/b-protein complexes was impaired.Fluorescence intensity of the CPC solution also shows a regular variation among experimental materials.Maximum fluorescence peak is in 683 nm.Relative fluorescence intensity is the highest in the normal leaves of standard,pure albino mutant is the lowest and the stripe leaf mutant is intermediate.Fluorescence scanning also indicate that peak V possesses the character similar to the CPI.From this we suggest that V peak might be CPa.

本试验采用玉米白色叶片及条纹叶片两个突变系和正常自交系的叶片对比进行叶绿素蛋白质复合体的研究。结果表明,两个突变系的CPI峰,均明显{氐于对照。CPII的变化,突变种中更为明显,条纹叶突变系的CPII峰低于对照而高于带有部分绿色组织的白色叶片,而纯白色叶片中则完全没有显示CPII峰。这表明,突变系中,尤其是纯白色突变系中捕光叶绿素a/b蛋白质的合成是受阻的。 此外,荧光原位扫描结果表明,靠近游离色素的v带,具有同CPI相似的性质,故而推断V带可能是CPa。文中对此进行了讨论。

This paper is our second report dealing with the research on the chlorophyll-protein complexes of chloroplast mutants.As experimental materials: one is a pale-green mutant,and the other is a yellow-green one.Both were induced from cultivated variety "Hedist" by r-ray radiation and introduced from Beijing Agriculture University.Methods of sample preparation and electrophoresis were the same as described previously.After electrophoresis the gel columns were scanned by photo-densitometer and fluorescence in situ...

This paper is our second report dealing with the research on the chlorophyll-protein complexes of chloroplast mutants.As experimental materials: one is a pale-green mutant,and the other is a yellow-green one.Both were induced from cultivated variety "Hedist" by r-ray radiation and introduced from Beijing Agriculture University.Methods of sample preparation and electrophoresis were the same as described previously.After electrophoresis the gel columns were scanned by photo-densitometer and fluorescence in situ and the absorption spectra determined.Results obtained indicate:1.After SDS-PAGE six pigment bands were separated in control.According to migration rate they are designed as 1,2,3,4,5 and 6.Results of photo-densitometer scanning with two wavelengths 675 nm and 650 nm also show six peaks respectively.Under 675 nm the 1st and 2nd peaks are much higher than that of 650 nm in all three experimental materials.This means that 1st and 2nd are CPI.The 3rd,fourth and fifth peaks do not show any remarkable differences between the pale-green mutant and the control,but they are absent completely in the yellow-green mutant.They may be CPU.The sixth peak migrating fastest in electrophoresis is the band of free pigment.2.Tests of absorption spectra reveal that the 1st and 2nd bands of all three materials show similar maximum absorption spectra of chlorophyll a.They are undoubtedly CPI,whereas the maximum absorption spectra of the 3rd,4th and 5th bands are similar to each other as that described by Thornber.They are CPII definitely.Owing to the yellow-green mutant has no 3,4 and 5 peaks,the determination of absorption spectra could only be undergone between the pale-green mutant and the control.3.Results of fluorescence scanning in situ under room temperature reveal that all of the three materials do not indicate any fluorescence emission of CPI,because the CPI content is mainly chlorophyll a.The pale-green mutant and the control show similar fluorescence emission of 3,4,5 bands which is absent completely in yellow-green mutant.Based on the results mentioned above we may conclude that the yellow-green mutant is a chloroplast mutant in which synthesis of chlorophyll b is impaired,so that it has no light-harvesting chlorophyll a/b-protein complexes (CPII).The pale-green mutant is not the real chloroplast mutant which possesses the normal chlorophyll-protein complexes.

本工作以六稜裸大麦“矮秆齐”及其γ射线诱发的两个叶绿体突变种“黄绿1832c”及“浅绿1834C”为材料,利用SDS聚丙烯酰胺凝胶电泳(PAGE)方法比较研究了它们的叶绿素蛋白质复合体。电泳结果表明:对照及浅绿突变体呈现6条带,依其迁移率由慢至快定为1、2、3、4、5及6带。这些带经光密度扫描,吸收光谱以及荧光原位扫描测定,所得结果彼此一致地证明,1、2带为CPI_3、4、5带为CPⅡ;6带为游离色素带。黄绿突变体为真正的叶绿体突变体,它不含3、4、5带,这表明它的叶绿素b的合成是受损害的;浅绿突变体没有发生明显的叶绿体突变,它具有和对照基本一致的叶绿素蛋白质复合体。

To facilitate the recognition of structural aberrations in interphase cytogenetics a study was performed in which premature chromosome condensation (PCC) was combined with fluorescent in situ hybridization (FISH). As a model male human peripheral blood cells were used. Prematurely condensed chromosomes were formed in these cells by using mitotic Chinese hamster cells a,s inducers .When fluorescent in situ hybridization was done on slides by using a total human DNA as probe, only PCC derived from human cells...

To facilitate the recognition of structural aberrations in interphase cytogenetics a study was performed in which premature chromosome condensation (PCC) was combined with fluorescent in situ hybridization (FISH). As a model male human peripheral blood cells were used. Prematurely condensed chromosomes were formed in these cells by using mitotic Chinese hamster cells a,s inducers .When fluorescent in situ hybridization was done on slides by using a total human DNA as probe, only PCC derived from human cells were painted with positive fluorescent signal whereas chromosome of Chinese hamster cells negative.

为便于对间期细胞染色体结构异常的识别,作者开展了对早熟凝缩染色体技术(PCC)与荧光原位杂交技术(FISH)二者相互结合起来的研究。作为模型,人外周血细胞在与诱导细胞——分裂期中国仓鼠孵巢细胞(CHO)融合后,形成了早熟凝缩的染色体。当使用人类全基因组DNA为探针做原位杂交时,仅见由人外周血细胞形成的PCC上被染上阳性荧光信号,CHO细胞染色体阴性。在使用人Y染色体特异性DNA探针作原位杂交时,结果不仅在男性外周上细胞核上,而且在一条由胞核演变的早熟凝缩染色体上,获得了特异性阳性荧光杂交信号。这一方法拓展了间期细胞遗传学的研究深度。

 
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