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胞浆内抗原
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  cytoplasmic antigen
     Objective To evaluate the method of marking cytoplasmic antigen ( CD3, CD22, MPO) and their clinical significance.
     目的 探讨和明确检测胞浆内抗原CD3、CD22、MPO的检测方法及其在白血病诊断中的意义。
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     Methods The cytoplasmic antigen CyCD79a was detected in 134 patients with AML,31 patients with T-ALL and both CyCD79a and CyCD22 were detected in 83 patients with B-ALL by flow cytometry.
     方法采用直接免疫荧光法对134例急性髓细胞白血病(AML)及31例T细胞型急性淋巴细胞白血病(T-ALL)患者骨髓或外周血进行胞浆内抗原CyCD79a标记染色,83例B-ALL患者同时进行胞浆内抗原CyCD79a和CyCD22标记染色,以CD45/SSC设门法进行三色流式细胞术分析。
短句来源
     Methods Immunofluorescence labeled with three and two color monoclonal antibodies directly were used to analyze the surface antigen and cytoplasmic antigen. Cytoplasmic antigen was detected by flow cytometry additionally.
     方法 细胞膜抗原采用单克隆抗体三色直接免疫荧光标记法 ,胞浆内抗原采用双色直标法用流式细胞术测定。
短句来源
  cytoplasmic antigens
     Detection of cytoplasmic antigens CD_ 3,CD_(22), MPO and it′s clinical significance
     胞浆内抗原CD_3、CD_(22)、MPO的检测方法及临床意义
短句来源
     Methods Single? or dual? color monoclonal antibodies directly labeled with immunofluorescence were used to analyze the cellular surface and cytoplasmic antigens and the detections of P170 glycoprotein were performed with indirect single?
     方法 采用全血单克隆抗体直接免疫荧光标记法及流式细胞术检测细胞表面抗原和胞浆内抗原 ,P170检测采用全血单色间接免疫荧光标记法 ,染色体核型分析采用短期细胞培养胰酶消化G显带技术。
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  “胞浆内抗原”译为未确定词的双语例句
     MAGE proteins distribute at two types in NSCLC tissues and are cytoplastic antigens.
     MAGE蛋白在NSCLC组织间有两种分布形式,属于胞浆内抗原
短句来源
     Results The B-acute lymphoblastic leukemia( B-ALL) and acute myeloid leukemia (AML) cases were found to be false positive for CD3 ( Becton Dickinson) and negative for CD3 ( Immunotech) .
     结果 急性B淋巴细胞性白血病(B-ALL)、急性髓细胞性白血病(AML)标本使用抗CD3抗体(Becton Dickinson公司)进行胞浆内抗原检测时,出现假阳性的结果;
短句来源
     Objective:To explore the examinational method of CyCD 3,CyCD 22 .MPO and the clinical significance.
     目的 :探讨和明确胞浆内抗原 CD3、CD2 2 、MPO的检测方法及其在白血病诊断中的意义。
短句来源
     Methods Two or one color monoclonal antibodies was used to label whole blood directly, flow cytometry were used to detect, the criteria for classification was according to antibody integral system.
     方法 细胞膜抗原采用全血单克隆抗体直接双标记法 ,胞浆内抗原CyMPO ,CyCD3,CyCD2 2的检测在对细胞膜进行通透性处理后 ,采用单色荧光标记 ,流式细胞仪检测。 分型根据抗体积分系统。
短句来源
     CyCD 22, CyCD 3 and CyMPO were specifically expressed in B lineage, T lineage and myeloid lineage leukemia, respectively, and the first two could be more sensitively detected than they were on cell membrane surface.
     胞浆内抗原CD2 2 、CD3 及髓过氧化物酶 (MPO)分别特异地表达在B ALL、T ALL及AML ,并比胞膜抗原检测更为敏感。
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  相似匹配句对
     Cytoplasmic antigens should be examined in AL and MDS.
     应加胞浆抗原检测。
短句来源
     The antigen was localized in the cytoplasm.
     抗原定位于组织细胞浆
短句来源
     The cytosolic PKA activity was increased.
     胞浆 PKA活性升高。
短句来源
     The factor Ⅷ related antigen was tested by immunohistochemistry, it supported that the cultured cells was ECs.
     免疫组织化学检测细胞胞浆Ⅷ因子相关抗原呈阳性。
短句来源
     The antigen was situated in the cytoplasm of hepatocytes and cancerous cells,few of which distributed around the nuclei.
     抗原定位于肝细胞和肝癌细胞的胞浆,少数有围核分布的特点。
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  cytoplasmic antigen
The MAb 4G3 interacted with cytoplasmic antigen of ectodermal epithelia-muscular cells of the both hydra species.
      
HLA B27, B51 and anti-neutrophile cytoplasmic antigen (ANCA) were searched in all the patients.
      
The results of these experiments confirm that most Toxoplasma surface antigens are protein in nature, and are consistent with the hypothesis that at least one cytoplasmic antigen is secreted onto the parasite cell surface.
      
Choice of fixation and denaturation for the triple labelling of intra-cytoplasmic antigen, bromodeoxyuridine and DNA
      
A triple staining method of intra-cytoplasmic antigen, bromodeoxyuridine (BrdU), and DNA for fluorescence image analysis is described.
      
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  cytoplasmic antigens
Although the precise trigger(s) for this process remain unknown, distinct clinical subsets exist that are characterized by antibodies directed against specific nuclear and cytoplasmic antigens including Jo-1 (histidyl-transfer RNA synthetase).
      
Isolation and characterization of cell wall and cytoplasmic antigens and peripheral blood leukocyte responses in rabbits immuniz
      
The results of this study show that both cell-wall and cytoplasmic antigens of C.
      
Aside from the two major ANCA antigens, a number of neutrophil cytoplasmic antigens recognized by ANCA have been identified, including human leukocyte elastase, lactoferrin, CAP57, and cathepsin G.
      
They recognized cytoplasmic antigens as judged by immunofluorescence examination of fixed cells or by immunoperoxidase staining of cancer tissues, but could never be visualized by immunofluorescence on the surface membrane of culture cells.
      
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Objective : To study the replication of hemorrhagic fever with renal syndrome(HFRS)virus incultured human glomerular mesangial cells and the effects of HFRS virus on human glomerular mesangialcells. Methods :The 76-118 strain, SR-11 strain and Chen strain of HFRS virus were inoculated into the cul-tured human glomerular mesangial cells, Results : It was found that the virus antigens could be detected inthe first passage by immunofluorescence technique, and that the increments of the detectable positive cellsand...

Objective : To study the replication of hemorrhagic fever with renal syndrome(HFRS)virus incultured human glomerular mesangial cells and the effects of HFRS virus on human glomerular mesangialcells. Methods :The 76-118 strain, SR-11 strain and Chen strain of HFRS virus were inoculated into the cul-tured human glomerular mesangial cells, Results : It was found that the virus antigens could be detected inthe first passage by immunofluorescence technique, and that the increments of the detectable positive cellsand viral antigens in the cytoplasm of infected cells were consistent with the prolongation of culture and fre-quence of passages. By phase contrast microscope, no obvious difference in morphology was found betweenthe infected cells and the control cells in the course of three times. Conclusions: These results indicated thatHFRS virus could infect cultured human glomerular mesangial cells and replicate in it。

目的:为探讨肾综合征出血热病毒(HFRSV)能否感染体外培养的人肾小球系膜细胞及在其中复制,能否引起细胞病变。方法:用HFRSV76-118株、SR-11株和陈株分别攻击体外传代培养的人肾小球系膜细胞。结果:于培养第一代即在受染细胞的胞浆中检出特异性病毒抗原,阳性细胞数及胞浆内抗原的含量随传代次数的增加而增加.感染细胞在光镜下未见明显病理损害。结论:HFRSV可以感染体外培养的人肾小球系膜细胞并可以在其中复制。

Objective To evaluate the method of marking cytoplasmic antigen ( CD3, CD22, MPO) and their clinical significance. Methods After permeabilizaed, the cells were marked by antibodies according to indirected immological fluorescein method. Then calculating the percentage of positive cells, the results of membrane marked method and cytoplasmic marked method were compared. Results In all leukemic patients, 11/19 expressed MPO, 6/19 expressed CyCD22, 1/19 expressed CyCD3, and 1/19 did not express above three antigens....

Objective To evaluate the method of marking cytoplasmic antigen ( CD3, CD22, MPO) and their clinical significance. Methods After permeabilizaed, the cells were marked by antibodies according to indirected immological fluorescein method. Then calculating the percentage of positive cells, the results of membrane marked method and cytoplasmic marked method were compared. Results In all leukemic patients, 11/19 expressed MPO, 6/19 expressed CyCD22, 1/19 expressed CyCD3, and 1/19 did not express above three antigens. Considering the results of morphology and membrane antigens, all the patients were clearly diagnosed. Conclusion The method of marking cyroplasmic antigens (CyCD22 ,CyCD3, MPO) is simple, accurate and senstive, and it has significance in diagnosing leukemia and could be used as a parameter for differentia] diagnosing leukemia.

目的 探讨和明确检测胞浆内抗原CD3、CD22、MPO的检测方法及其在白血病诊断中的意义。方法 对细胞膜进行渗透性处理后,采用单抗间接荧光免疫标记细胞内相应的抗原,在荧光显微镜下计数,并将结果与形态学和胞膜标记结果进行比较。结果 19例初诊为白血病患者中,11例MPO阳性,6例C_yCD22,1例阳性C_yCD3阳性,1例3项均阴性,结合形态学和表面抗原分析,全部病例获得明确诊断。结论 胞浆内CD3、CD22、MPO的检测简单、准确、灵敏度高,对白血病诊断和鉴别诊断具有重要意义,可在临床广泛开展使用。

Objective:To explore the examinational method of CyCD 3,CyCD 22 .MPO and the clinical significance. Method:After immobilization of cell membrane,the cytoplasmic antigens were marked fluorescence indirectly by using monoclonal antibody.Then the qualitative and quantitative analysis was performed on fluorescent microscopy. Lastly we compared the results with the related morphology and expression of surface antigens.Result:In 19 atients,11 were MPO(+),6 were CyCD 22 (+),1 was CyCD 3(+),only 1 was negative...

Objective:To explore the examinational method of CyCD 3,CyCD 22 .MPO and the clinical significance. Method:After immobilization of cell membrane,the cytoplasmic antigens were marked fluorescence indirectly by using monoclonal antibody.Then the qualitative and quantitative analysis was performed on fluorescent microscopy. Lastly we compared the results with the related morphology and expression of surface antigens.Result:In 19 atients,11 were MPO(+),6 were CyCD 22 (+),1 was CyCD 3(+),only 1 was negative for all cytoplasmic antigens. All patients acquired clear diagnosis.Conclusion:The method for detecting cytoplasmic antigens is simple and clear and high sensitive. It can an important role in the diagnosis and distinguishing diagnosis of leukemia.

目的 :探讨和明确胞浆内抗原 CD3、CD2 2 、MPO的检测方法及其在白血病诊断中的意义。方法 :采用多聚甲醛和甲醇联合固定细胞 ,单抗间接荧光法标记细胞内相应的抗原 ,在荧光显微镜下检测计数 ,并将结果与形态学和细胞表面抗原检测结果进行对照研究。结果 :19例初诊患者中 ,11例 MPO阳性 ,6例 Cy CD2 2 阳性 ,1例Cy CD3阳性 ,1例 3项均为阴性 ,结合形态学和表面抗原分析 ,全部病例获得明确诊断。结论 :胞浆内 CD3、CD2 2 、MPO的检测简便、准确、灵敏度高 ,可在临床广泛开展使用 ,对白血病的诊断和鉴别诊断具有重要意义

 
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