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内毒素et
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     The changes of serum endotoxin(ET),TNF-α,IL-1β,IL-6 and IL-10 in different groups and different time points were observed.
     动态观察同组不同时间,不同组同时间的血清中内毒素(ET)、TNF-α、IL-1β、IL-6和IL-10含量变化。
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     Results After treating with AD,the levels of TBiL,TBA,NH3,Cr,ET,NO,TNF-α,IL-6 decreased signicantly(P < 0.05),PTA increased(P < 0.05).
     结果AD治疗后,血总胆红素(TBiL)、总胆汁酸(TBA)、氨(NH3)、尿素氮(Bun)、肌酐(Cr)、内毒素(ET)、一氧化氮(NO)、肿瘤坏死因子(TNF-α)和白细胞介素-6(IL-6)浓度明显下降(P<0.05);
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     Methods The clinical data of 20 patients with obstructive jaundice were collected and the measurement of serum TNF-α,ALT, ALP, endotoxin and IGF-1 were performed.
     方法收集20例梗阻性黄疸患者的临床资料,并对患者血清TNFα、肝功能指标(ALT、ALP、TB)、内毒素(ET)及IGF1进行检测。
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     The plasma levels of endotoxin,TNF-α,IL-6,IL-8 and NO were detected before and after shock. The survival rates of 12 h,24 h and 48 h were observed.
     观察休克前后血浆内毒素(ET)、肿瘤坏死因子(TNF)-α、白介素(IL)-6、IL-8、一氧化氮(NO)的变化,以及动物12h、24h、48h的存活率。
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     Objective To study the changes in activity of phospholipase A_2 (PLA_2) in the course of endotoxin (ET)-induced acute lung injury (ALI) in rabbits and the antagonizing effect of fructose-1,6-diphosphate (FDP), in order to evaluate the therapeutic effect of FDP on ET-induced ALI.
     目的研究内毒素(ET)致大耳白兔急性肺损伤(ALI)过程中磷脂酶A2的变化和1,6-二磷酸果糖(FDP)的拮抗作用,探讨FDP对ET所致ALI的治疗作用。
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     Wu et Y.
     W u et Y.
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     et C.
     et C.
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     et Saccado.
     et Saccado)
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     zanthoxyli Sun et Bai.
     et Bai))
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     Methods for Endotoxin Removal
     内毒素的去除策略
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  endotoxin (et)
The involvement of intestinal flora endotoxin (ET) in the regulation of immune homeostasis, namely, the maintenance of the physiological tone of all components of the immune system (so-called systemic endotoxinemia, SEE) is postulated.
      
The content of SA-pretreated endotoxin (ET) was quantitatively determined using Limulus test.
      
The effect of tumor necrosis factor-α (TNF-α) on endotoxin (ET)-mediated lung damage caused by incomplete ligation of large intestine and the influence of free Fu on the expression of TNF-α mRNA were explored.
      
Endotoxin (ET) and cytokine levels following programmed peritoneal lavage (PPL) treatment of peritonitis in association with tau
      
Therefore, in this study, tumor-necrosis-factor-α (TNF-α), interleukin-6 (IL-6), and endotoxin (ET) levels were determined in the blood taken from the hepatic veins of 31 patients who underwent OLT.
      
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The mechanism of hemadynamic changes associated with endotoxic shock continues to be an elusive medical problem.The mechanism of falling of blood pressure inendotoxic shock has been of particular concern.A series of 24 adult dogs anesthetized with pentobarbital sodium 30mg/kg wereused in this study.Blood pressure,central venous pressure,platelets and WBC count,hematocrit,blood viscosity were monitored at 0,5,15,60,120,240,360 minutesafter injection of lethal dose of disintegrated E.Coli.In response to the injection,a...

The mechanism of hemadynamic changes associated with endotoxic shock continues to be an elusive medical problem.The mechanism of falling of blood pressure inendotoxic shock has been of particular concern.A series of 24 adult dogs anesthetized with pentobarbital sodium 30mg/kg wereused in this study.Blood pressure,central venous pressure,platelets and WBC count,hematocrit,blood viscosity were monitored at 0,5,15,60,120,240,360 minutesafter injection of lethal dose of disintegrated E.Coli.In response to the injection,a number of pathological alterations were producedincluding falling of B.P.,CVP,platlets count,WBC count and increasing of he-matocrit and viscosity.In the course of experiment,there was first an initial stage ofshock;second,an apparent recovery;and third,the terminal phase of shock.Of particular interest is that the occurrence of microaggregates about 20~40 mic-rons in size composed chiefly of platelets was recognized by blood smear.It occuredin blood just 5 minutes after the injection of disintegrated E.Coli,and was conc-omitant with the drop of platelets and WBC(Polys).Microembolic blockage by platel-ets and WBC(polys)aggregates would appear to be the major causative factors infalling of B.P.and C.V.P.et al.,At the ends of experiment about 6 hrs afterinjection of disintegrated E,Coli,the majority of animals died of respiratory failure,The cause of multiple organ failure viewed by pathological examination may be releaseof some vasoactive substances from platelets and WBC(Polys).In conclusion,the agg-regates formation may play an impartant role in the mechanism of endotoxie shock.

本文从血液动力学、血粘度、血象及形态学研究了24条狗内毒素休克发生机制。动物于注射内毒素(ET)后5分钟内血中即出现以血小板为主的微聚物,同时血中血小板、嗜中性白细胞、血压及中心静脉压均下降,血球压积增加,肺中出现微血栓,动物呈呼气性呼吸困难。此变化迅即缓解,动物于 ET 后30′~120′内上述指标逐渐部分恢复。4~6小时内血中又再度出现大量不易解聚的微聚物,上述指标变化加重而导致大部分动物于6小时内死亡,对照组无上述变化。此实验说明微聚物形成在内毒素休克发生中具有重要意义。

In order to study mechanisms of endotoxin (ET) and endogenous pyrogen (EP) byperthermic ceilings, the febrile responses induced in rabbits by intravenous injection of varying doses of EP were observed, and the doses in which the febrile reactions of rabbits to ET and EP are limited by hyperthermic ceiling were identified. Furthermore, the febrile reactions of typerttermic ceiling plus different pyrogen load were observed. T, e experiment stowed: 1. The level of ET hypert ermic ceiling is notably higher in comparison...

In order to study mechanisms of endotoxin (ET) and endogenous pyrogen (EP) byperthermic ceilings, the febrile responses induced in rabbits by intravenous injection of varying doses of EP were observed, and the doses in which the febrile reactions of rabbits to ET and EP are limited by hyperthermic ceiling were identified. Furthermore, the febrile reactions of typerttermic ceiling plus different pyrogen load were observed. T, e experiment stowed: 1. The level of ET hypert ermic ceiling is notably higher in comparison to that of EP hypertiermic ceiling. The findings indicated that the mechanisms of ET fever and ET Lyperthermic ceiling are different from those of EP fever and EP hyperthermic ceiling. 2. After EP hyperthermic ceiling was added the load of ET. the altitude of fever exceeded the EP hyperthermic ceiling; but after ET hypertthermic ceiling was added the load of EP, the altitude couldn't exceed ET hyperthermic ceiling. The results suggested that ET hyperthermic ceiling contained EP hyperttermic ceiling. The latter was an important component of ET hyperttermic ceiling, but there are other factors participating in the mechanisms of ET fever and ET hyperthermic ceiling except EP.

为研究内毒素(ET)和内生致热原(EP)热限的形成机制,观察家兔静脉注射不同剂量ET和EP的发热效应。确定ET及EP的热限剂量后对ET与EP热限的发热水平以及热限加不同致热原负荷时发热水平的变化进行观察比较。实验表明:1.ET热限水平明显高于EP热限水平,提示ET性发热及热限的形成机制与EP性发热及热限形成机制有所不同。2.EP热限加ET负荷使发热高度突破了EP热限,而ET热限加EP负荷则未能使发热高度超过ET热限的水平。表明ET热限包含了EP热限,即EP热限是构成ET热限的重要成因,但不是全部。ET性发热及热限的机制中还有EP以外的因素参与。

In order to determine whether the endogenous crygen(EC)inhibits the second phase of ET-fever, the following observations were carried out: 1. Effect of the infusion of human urine on the body temperature of normal rabbits, changes of cAMP content in plasma and CSF during the EC active stage; 2. Effect of the infusion of human urine orequal volume of normal saline on the second phase of ET-fever, changes of the cAMP level in plasma and CSF during EC active stage. The results showed: 1. The rectal temperature...

In order to determine whether the endogenous crygen(EC)inhibits the second phase of ET-fever, the following observations were carried out: 1. Effect of the infusion of human urine on the body temperature of normal rabbits, changes of cAMP content in plasma and CSF during the EC active stage; 2. Effect of the infusion of human urine orequal volume of normal saline on the second phase of ET-fever, changes of the cAMP level in plasma and CSF during EC active stage. The results showed: 1. The rectal temperature of normal rabbits decreased markedly after human urine was given I. V., but not after normal saline I. V. The cAMP content in plsma and CSF decreased markedly after urine or saline was given. These findings indicated the cAMP level did not participate in the mechanism of the action of EC; 2. Formation of second phase of ET-fever was inhibited by urine infusion, level of hyperthermie ceiling also decreased and the biphasic fever was transformed to monophasic, but not by normal saline infusion. The cAMP content in plasma and CSF all decreascd after urine or saline I.V. infusion, but its effect on urine was not so significant as normal saline. These findings indicatd that inhibited action of EC was not related apparently to the cAMP level.

为验证内生致冷原(EC)能否影响内毒素(ET)性发热第二热相或热限水平,并确实脑脊液中cAMP水平是否与EC的降温作用有关,作者用90只新西兰兔进行实验。观察:①输注人尿或等量生理盐液对正常家免体温的影响,检测EC效应期血浆和脑脊液中cAMP的含量;②在第二热峰出现前输注人尿或生理盐液对第二热相的影响,检测EC效应期血浆及脑脊液中cAMP的含量。结果表明:①人尿明显降低正常家兔的直肠温度,而等量生理盐液则无此作用,且两者均引起血浆及脑脊液中cAMP浓度的明显下降,提示EC的降温作用与脑cAMP浓度下降可能无重要关系;②人尿(EC)抑制ET性发热第二热相的形成,从而降低热限水平,变双相热为单相热,同量生理盐水无此作用,两者都能降低血浆和脑脊液中cAMP的水平,但EC不及NS明显,表明EC抑制第二热相或降低热限水平的作用也与cAMP浓度变化无重要关系,作者推论cAMP不是ET性发热第二热相的唯一成因。

 
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