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耐热植酸酶
相关语句
  thermostable phytase
     Clone and Expression of Thermostable Phytase Gene in Bacteria
     细菌耐热植酸酶基因的克隆及表达
短句来源
  “耐热植酸酶”译为未确定词的双语例句
     Cloning of Heat-Stable PhyA Gene and Construction of its New Expression Vector
     耐热植酸酶基因phyA的克隆及新型表达载体的构建
短句来源
  相似匹配句对
     Clone and Expression of Thermostable Phytase Gene in Bacteria
     细菌耐热植酸酶基因的克隆及表达
短句来源
     STUDY OF LABORATORY EVALUATION METHOD FOR PHYTASE'S TEMPERATURE-RESISTANT
     植酸酶耐热性实验室评价方法的研究
     A study of phytase and its application
     植酸酶及其应用
短句来源
     The Research Development of Phytase
     植酸酶的研究进展
短句来源
     Heat-proof PVC resin
     耐热PVC树脂
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  thermostable phytase
Thermostable phytase production by Thermoascus aurantiacus in submerged fermentation
      
Production of high activity thermostable phytase from thermotolerant Aspergillus niger in solid state fermentation
      
A novel thermostable phytase gene was cloned from Aspergillus fumigatus WY-2.
      
Bacillus species producing a thermostable phytase was isolated from soil, boiled rice, and mezu (Korean traditinal koji).
      


Objective To construct a fusion expression plasmid containing heat-stable phytase A gene from A.fumigatus for further expression of phytase A protein in A.niger.Methods Genomic DNA of A.fumigatus was isolated and the phytase A coding sequence was amplified by PCR.The amplified fragment was inserted into the expression vector pYG 1.2 in frame with glucoamylase coding sequence using the promoter from A.niger glaA.The DH5αwere transformed and the transformants were confirmed by PCR.The positive products were...

Objective To construct a fusion expression plasmid containing heat-stable phytase A gene from A.fumigatus for further expression of phytase A protein in A.niger.Methods Genomic DNA of A.fumigatus was isolated and the phytase A coding sequence was amplified by PCR.The amplified fragment was inserted into the expression vector pYG 1.2 in frame with glucoamylase coding sequence using the promoter from A.niger glaA.The DH5αwere transformed and the transformants were confirmed by PCR.The positive products were verified by restriction enzyme digestion and sequencing.Results A DNA fragment of approximately 1.4kb was generated by PCR and cloned into plasmid pYG 1.2.The sequence cloned shares 99.9% homology with reported phytase A gene sequence except for two base difference.Conclusion The recombined plasmid containing heat-stable phytase was constructed successfully and it will be used to express heat-stable phytase A in large quantity in our A.niger expression system.

目的 克隆烟曲霉耐热植酸酶基因 ,构建分泌性的真核表达质粒 ,以获取耐热的基因工程植酸酶。方法 提取烟曲霉菌的基因组 ,以此为模板PCR扩增成熟肽编码序列 ,双酶切后与包含黑曲霉葡萄糖淀粉酶启动子和基因片断的真核表达载体 pYG 1 .2相连接 ,转化入大肠杆菌DH5α ,从转化菌株中提取质粒进行序列测定和分析。 结果 PCR扩增出 1 32 6bp的植酸酶编码序列 ,插入 pYG 1 .2质粒中 ,构建了重组质粒 ,重复实验结果表明 ,该植酸酶基因序列与已报道的烟曲霉植酸酶序列有 2个碱基的差异 ,编码的蛋白质序列有 99.8%的同源性。结论 成功构建了用于黑曲霉菌真核表达耐热植酸酶的重组质粒。

 
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