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再生杂种植株
相关语句
  somatic hybrid plant regeneration
     SOMATIC HYBRID PLANT REGENERATION BETWEEN NAVEL ORANGE AND LEMON VIA PROTOPLAST ELECTRO FUSION
     脐橙与柠檬种间细胞电融合再生杂种植株
短句来源
  “再生杂种植株”译为未确定词的双语例句
     INTERGENERIC SOMATIC HYBRID PLANTS OF NICOTIANATABACUM L. AND LYCIUM BARBARUM L. BY PROTOPLAST ELECTROFUSION
     烟草和枸杞属间原生质体电融合再生杂种植株
短句来源
     Production of Somatic Hybrid Plants Between Two Types of Wheat Protoplasts and the Protoplasts of Haynaldia villosa
     同一小麦品种不同来源的两种原生质体与簇毛麦原生质体融合再生杂种植株(英文)
短句来源
     All the regenerated clones were confirmed as somatic hybrids by cytological, isozyme, chromosome and random amplified polymorphic DNA (RAPD) analysis.
     经过形态学、同工酶、染色体和RAPD分析 ,确定其全部为属间体细胞杂种。 其中的 5个克隆再生杂种植株
短句来源
     Amphidiploid(2n=28,DDRR) of Triticum tauschii and Secale cereale was synthesized through culture of immature hybrid embryos and colchicines treatment.
     将节节麦与黑麦杂交,利用F1胚愈伤组织培养再生杂种植株,对胚再生杂种F1进行秋水仙素加倍获得了节节麦和黑麦的双二倍体(DDRR). 杂种F1(DR)的减数分裂中期Ⅰ (PMCMI)染色体构型平均为 2n=10. 16Ⅰ +1. 86Ⅱ+0. 12Ⅲ+0. 00625Ⅳ.
短句来源
  相似匹配句对
     Tissue Culture and Plantlet Regeneration of Iris hybrids
     杂种鸢尾的组织培养和植株再生
短句来源
     EMBRYO RESCUE AND PLANT REGENERATION OF INTERSPECIFIC HYBRIDIZATION BETWEEN S. IND ICU M AND A WILD SPECIE S. SCHIZINIXIANU M
     芝麻远缘杂种胚胎的营救和植株再生
短句来源
     POLLEN MESOPHYLL PROTOPLAST FUSION AND HYBRID PLANT REGENERATION IN NICOTIANA
     烟草属花粉-叶肉原生质体的融合及杂种植株再生
短句来源
     INTERGENERIC SOMATIC HYBRID PLANTS OF NICOTIANATABACUM L. AND LYCIUM BARBARUM L. BY PROTOPLAST ELECTROFUSION
     烟草和枸杞属间原生质体电融合再生杂种植株
短句来源
     THE BARLEY x WHEAT CROSSES AND THEIR OFFSPRING PLANTS
     栽培大麦×普通小麦杂种及其再生植株
短句来源
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  somatic hybrid plant regeneration
This is the first report of hexaploid somatic hybrid plant regeneration from fusion between diploids in Aurantioideae.
      


Hybridizations betweenAegilops tauschii(2n=14) andHordeum vulgare (2n=14) were made using Ae. tauschii as female. The mean frequency of seed set was 3111% in two cross combinations, 13 hybrid embryos were cultured for inducing callus, of which 2 produced totipotent callus, and plants were regenerated from them. These plants were Ae. tauschii-H. vulgare amphidiploids with doubling chromosome number (2n=28) and octoploids with repeatedly doubling chromosome number (2n =50~56). The Ae. tauschii-H. vulgare ...

Hybridizations betweenAegilops tauschii(2n=14) andHordeum vulgare (2n=14) were made using Ae. tauschii as female. The mean frequency of seed set was 3111% in two cross combinations, 13 hybrid embryos were cultured for inducing callus, of which 2 produced totipotent callus, and plants were regenerated from them. These plants were Ae. tauschii-H. vulgare amphidiploids with doubling chromosome number (2n=28) and octoploids with repeatedly doubling chromosome number (2n =50~56). The Ae. tauschii-H. vulgare amphidiploids were sterile and their average chromosome pairing at MI were 1479Ⅰ+195Ⅱ+453Ⅱ'+001Ⅲ.Some octoploid (2n =56) plants produced seeds after selfing.

以节节麦(2n=14)为母本和大麦(2n=14)进行杂交,两组合平均结实率为3111%。对13个幼胚进行愈伤组织诱导培养,其中2个形成全能性愈伤组织进而分化出再生杂种植株。细胞学观察表明,杂种胚再生植株均是染色体加倍的节节麦-大麦双二倍体(2n=28)或染色体再加倍的多倍体。节节麦-大麦双二倍体在减数分裂中期Ⅰ染色体配对平均构型为1479Ⅰ+195Ⅱ+453Ⅱ'+001Ⅲ,表现为自交不育。杂种胚再生植株染色体数目再加倍的多倍体有少数产生了自交种子。

Psathyrostachys juncea is a potential source of useful genes, such as the barley yellow dwarf virus resistance, salt tolerance and drought tolerance, for wheat improvement. Conventional sexual hybridization between wheat and Psathyrostachys juncea is very difficult to occur as the two are sexual incompatible. Somatic hybridization is a promising technique for creating hybrids across the sexual border. Here we report a fusion system for somatic hybridization of wheat using PEG method. Mixed protoplasts...

Psathyrostachys juncea is a potential source of useful genes, such as the barley yellow dwarf virus resistance, salt tolerance and drought tolerance, for wheat improvement. Conventional sexual hybridization between wheat and Psathyrostachys juncea is very difficult to occur as the two are sexual incompatible. Somatic hybridization is a promising technique for creating hybrids across the sexual border. Here we report a fusion system for somatic hybridization of wheat using PEG method. Mixed protoplasts of two wheat (Triticum aestivum L. cv. Jinan 177) culture cells (cha9 and 176) were used as the recipients to fuse with the donors, the protoplasts of Psathyrostachys juncea (Fisch.) Nevski irradiated with ultraviolet light (UV) at an intensity of 380μW/cm 2 for 1 min or 2 min. Sixteen clones were generated in the combination Ⅰ, (wheat 176 + wheat cha9 + P. juncea 1 min UV treatment) and five of the hybrid clones could differentiate to green plants. All the regenerated clones were confirmed as somatic hybrids by cytological, isozyme, chromosome and random amplified polymorphic DNA (RAPD) analysis. Chloroplast genome of the hybrids was analyzed using 7 pairs of wheat specific chloroplast microsatellite (SSR) primers. Three clones were obtained from the combination Ⅱ(wheat 176 + wheat cha9 + P. juncea 2 min UV treatment), and all browned slowly and died in 3 months. This result indicated that the mixed wheat cells was helpful to the formation and regeneration of hybrid callus and the dosage of the UV had significant effect on the development of the fusion products.

以小麦品种济南 1 77悬浮细胞系来源的原生质体与同品系胚性愈伤组织制备的原生质体混合后作为受体 ;以经过 380 μW cm2 紫外线照射 1min、2min的新麦草原生质体分别作为供体 ,用PEG法诱导融合。组合Ⅰ (1 76 +cha9+新麦草UV 1min)获得 1 6个再生克隆。经过形态学、同工酶、染色体和RAPD分析 ,确定其全部为属间体细胞杂种。其中的 5个克隆再生杂种植株。用 7对小麦SSR引物对杂种克隆的叶绿体基因组进行了分析 ;组合Ⅱ (1 76+cha9+新麦草UV 2min)只获得 3个克隆 ,且逐渐褐化死亡。表明以小麦济南 1 77的两种培养细胞混合作受体的融合体系有利于杂种的获得及再生 ;紫外线对融合产物的生长发育有明显的剂量效应

Amphidiploid(2n=28,DDRR) of Triticum tauschii and Secale cereale was synthesized through culture of immature hybrid embryos and colchicines treatment. Through the researching to the PMC MI chromosome pairing configurations of the embryo regeneted F1 and the amphidiploid were 10.16Ⅰ+1.86Ⅱ+0.12Ⅲ+0.00625Ⅳ and 8.676Ⅰ+9.318Ⅱ+0.019Ⅲ+0.004Ⅳ, respectively.The mean seed sets of the amphidiploid C1 was 2.118%, which could be used as a new speices and materials to improve wheat.

将节节麦与黑麦杂交,利用F1胚愈伤组织培养再生杂种植株,对胚再生杂种F1进行秋水仙素加倍获得了节节麦和黑麦的双二倍体(DDRR). 杂种F1(DR)的减数分裂中期Ⅰ (PMCMI)染色体构型平均为 2n=10. 16Ⅰ +1. 86Ⅱ+0. 12Ⅲ+0. 00625Ⅳ. 该双二倍体(DDRR)的减数分裂中期Ⅰ(PMCMI)染色体构型平均为 2n=8. 676Ⅰ +9. 318Ⅱ+0. 019Ⅲ+0. 004Ⅳ,C1的结实率平均为 2. 118%. 该双二倍体可作为一个新的物种以及作为改良小麦的种质材料.

 
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