AIM: To establish human lymphocyte cell line secreting monoclonal antibody against Rhesus(D) antigen and analyse the nucleotide and deduced amino acid sequences of a human monoclonal anti-D Fab fragment.
Methods lymphocytes from peripheral blood in 16 leprosy patients in different periods were immunologically labeled by monoclonal antibodies from mouse CD 3/CD 4/CD 8,CD 3/CD (16+56) (NK) and CD 3/HLA-DR were determined by using flow cytometry(FCM).
2. ^Reagent: The primary antibodies p53 ( mouse monoclonal antibody, DO -7) and p63(mouse monoclonal antibody, 4A4 +63P02) ,SP kit and DAB developer were bought from Fuzhou Maixin.
Methods The lymphocytes gained from the peripheral blood of the patients in different intervals after hand allotransplantation were immunologically labeled by triple color fluorescent monoclonal antibodies CD 3/CD 4/CD 8 and dual color fluorescent monoclonal antibodies CD 3/CD (16+56) and CD 3/HLA DR.
METHODS: The experiment was conducted in the Cell Laboratory of Wuxi Third People's Hospital between May 2004 and October 2005.①Embryonic MSCs were isolated and cultured to identify its surface antigen, in which CD34-FITC,CD45-PE,CD19-FITC,CD29-FITC,CD44-PE,CD105-FITC, CD106-FITC, HLA-DR-FITC rat-anti-human monoclonal antibody were added respectively to analyze with flow cytometry.
Here we describe the self-association kinetics of human monoclonal immunoglobulin light chains λ (GRY) isolated from urine of a patient with multiple myeloma.
Additional studies have demonstrated that the pANCA human monoclonal antibody (mAb) Fab 5-3 reacts with histone H1 and with bacterial and mycobacterial antigens.
Rituximab is a chimeric mouse-human monoclonal antibody against the B cell-specific antigen CD20, which selectively and profoundly depletes B lymphocytes and has been widely used to treat B cell lymphomas.
The VH3 gene family, which encodes for approx 50% of immunoglobulins expressed by peripheral B-cells from normal adults, is underrepresented in human monoclonal antibodies to HIV-1 and in the peripheral B-cells of AIDS patients.
The lymphocytes isolated from blood of the patients with HFRS in the convalescence phase were transformed by EBV, then fused with the murine myeloma cell line X63-Ag8.653 and the heteromye-loma line SHM-D33. Two hybridoma lines, 86-1 and 86-2, were established which produce IgMA. human monoclonal antibodies to HFRSV at 30μg/ml and 50μg/ml in the spent media, respectively. They have been ptoducing the antibodies stably for 7 months. Ascitic fluids rich in human monoclonal antibodies were prepared by injectin...
We utilized OKT monoclonal anti-human T lymphocyte antibodies to determine the levels of T lumphocyte subsets in the peripheral blood of 36 normal persons and 16 patients with insulin-dependant diabetes mellitus.We found no marked difference in the proportions of OKT_3,OKT_4,OKT_8 and OKT_4/OKT_8 between the two groups(P>0.05).Nor was any marked difference found between the 9 children and 7 adults with IDDM group and the control(P>0.05).The T lymphocyte subsets were not related to the dosage of insulin used...
用抗人单克隆抗体 OKT 系列检测36例正常人和16例胰岛素依赖型糖尿病(IDDM)患者外周血 T 淋巴细胞亚群(OKT_3、OKT_4、OKT_8),计算 OKT_4/OKT_8之比。结果发现,正常人和胰岛素依赖型糖尿病患者之间无显著性差异(P>0.05);9例小儿和7例成人胰岛素依赖型糖尿病患者与对照组间亦无明显差异(P>0.05)。T 细胞亚群和胰岛素用量无相关性。
The techniques for preparing human monoclonal antibodies by human-mouse heterohybridomas were studied. The murine myeloma cells ( Sp2/0 ) were fused with human peripheral blood lymphocytes ( PBL ) immunized with tetanus toxoid(TT). The results indicated: ( 1 ) The total amount of B cells in PBL was increased by in vitro activation with PWM + TT and the fusion ratio was higher. The cell lines stably secr-eting the antibodies were obtained easily. (2 ) The precursor B cells capable of secreting specific anti-...
人单克隆
human monoclonal
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