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磷酸酶基因
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  phosphatase gene
     The Difference of Protein Tyrosine Phosphatase Gene Expression in Skeletal Muscle Tissue from Type-2 Diabetic Insulin Resistance Rats
     2型糖尿病胰岛素抵抗大鼠骨骼肌组织蛋白酪氨酸磷酸酶基因表达的改变
短句来源
     Cloning and Expression Characteristics of Protein Phosphatase Gene ZmPP2C of Zea mays Roots
     玉米根系蛋白磷酸酶基因ZmPP2C的克隆及表达特性
短句来源
     Molecular Cloning of a Rice Sphingosine-1-phosphate Phosphatase Gene, OsSPP1
     水稻鞘氨醇磷酸酶基因OsSPP1的克隆鉴定
短句来源
     PTEN gene (tensin homology and phosphatase deleted on chromosome ten, namely tensin and auxilin isogenesis, No. 10 chromosome lose phosphatase gene) is the firstly found phosphatase oncongen suppressor which regulating cell proliferation, transformation and adhesion etc.
     PTEN是第一个被发现具有磷酸酯酶活性的抑癌基因,全称是与细胞骨架蛋tensin同源在肿瘤第10号染色体丢失的磷酸酶基因,参与细胞增殖过程、转化、粘附的负性调控。
短句来源
     In control group, alkaline phosphatase gene that retroviral vector carried was transferred at injured site.
     对照组局部转导逆转录病毒载体携带的碱性磷酸酶基因;
短句来源
  “磷酸酶基因”译为未确定词的双语例句
     Objective To detect the expression of PTEN, PIP3 and cyclin D1 in oral squamous cell carcinoma and precancerous lesions and analyze their correlation.
     目的通过检测口腔鳞癌及癌前病变中抑癌基因与张力蛋白同源10 q丢失的磷酸酶基因(PTEN)、3、4、5-三磷酸磷脂酰肌醇(PIP3)、细胞周期蛋白D1(cyclin D1)的表达,分析其相关性,初步探讨在口腔鳞癌中PTEN可能的作用途径。
短句来源
     Method:Polymerase chain reaction,restriction enzyme digestion were used to screen the known mutations1388G→A,1376G→T,1360C→T,1024C→T,592C→T,517T→C,493A→G,487G→A,392G→T and95A→G in human G6PD gene. Single strand conformation polymorphism analysis was used to screen all the exons of G6PD gene. DNA sequencing was used to determine the mutations.
     方法:用聚合酶链反应、限制性内切酶筛查葡萄糖-6-磷酸酶基因1388G→A、1376G→T、1360C→T、1024C→T、592C→T、517T→C、493A→G,487G→A、392G→T、95A→G突变,用单链构象多态性筛查葡萄糖-6-磷酸脱氢酶基因的所有外显子,用核苷酸序列测定确定基因突变。
短句来源
     According to the cDNA sequence of AtPP2C gene(AT1g07430) in GenBank a pair of primer was designed and synthesized.
     根据GenBank中已发表的拟南芥蛋白磷酸酶基因AtPP2C(AT1g07430)的cDNA序列设计并合成了1对引物。
短句来源
     Sequence analysis and homology alignment showed that MD1 had 93% similarity with matK in maize chloroplast genome,a gene encoding maturase included in typeⅡ intron splicing of RNA transcript; MD2 had 99% similarity with gene PP2C,encoding serine / threonine protein phosphatase type 2C in extremely drought tolerant Sporobolus stapfianus; and MD3 had 99% similarity with rice gene encoding metacaspase,belonging to aspartic acid specific cysteine caspases.
     序列分析和同源性比对表明,MD1与玉米叶绿体基因组中编码参与RNA转录本Ⅱ型内含子剪切的成熟酶的matK基因有93%的相似性,MD2与极端耐旱的Sporobolus stapfianus的丝氨酸/苏氨酸2C型蛋白磷酸酶基因PP2C的相似性达99%,MD3与属天冬氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase基因有99%的相似性。
短句来源
     Comparison of Secreted Alkaline Phosphatase(SEAP) Expression Level from B16F1 Cells Using Cationic Lipid and Recombinant Adenovirus as Gene Transfer Vehicle
     基因转移载体脂质体和重组腺病毒介导碱性磷酸酶基因在B16F1细胞中的表达比较
短句来源
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  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     Cloning and Sequence Analysis of pNPPase
     对硝基酚磷酸酶pNPPase的基因克隆和序列分析
短句来源
     coli lac Z gene.
     colilacZ基因
短句来源
     Molecular Cloning of a Rice Sphingosine-1-phosphate Phosphatase Gene, OsSPP1
     水稻鞘氨醇磷酸酶基因OsSPP1的克隆鉴定
短句来源
     The duration of starvation had no infiuence on the amountof both alkaline and acid phosphatases.
     酸性磷酸酶无变化。
短句来源
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  phosphatase gene
The Meiothermus ruber alkaline phosphatase gene was cloned, expressed in Escherichia coli cells, and sequenced.
      
A cloning vector system was constructed on the basis of the pBR322 derivative pEG1 by introducing the whole parB locus of plasmid R1 cloned behind the promoter of the alkaline phosphatase gene (phoA) of Escherichia coli.
      
In addition, alkaline phosphatase gene expression and activity were dramatically increased upon BMP-6 treatment.
      
The chromosome VIII sequence appears to be a previously unnamed acid phosphatase gene that we propose to call PH012.
      
Thus, similar to the repeated SUC, MAL, X and Y' sequences, some members of the repeated acid phosphatase gene family also appear near the termini of yeast chromosomes.
      
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The nucleotide sequence of the promoter and the signal peptide of E. toli alkaline phosphotase gene from pFOG405 plasmid has been disclosed and compared with the sequence reported by Kikuchi et al. The result shows that the PvuⅡ-HpaⅡ fragment (144bp) containing the promoter and signal peptide sequence from pYK190 plasmid is sufficient for expression and secretion of foreign genes in E. coli. The deletion of the dyad sequence (26bp) from 5' end of the promoter region doesn't affect expression level of foreign...

The nucleotide sequence of the promoter and the signal peptide of E. toli alkaline phosphotase gene from pFOG405 plasmid has been disclosed and compared with the sequence reported by Kikuchi et al. The result shows that the PvuⅡ-HpaⅡ fragment (144bp) containing the promoter and signal peptide sequence from pYK190 plasmid is sufficient for expression and secretion of foreign genes in E. coli. The deletion of the dyad sequence (26bp) from 5' end of the promoter region doesn't affect expression level of foreign target genes The experiment also provids the useful information for construction of efficient expression and secretion vector using the PvuⅡ-HpaⅡ fragment and studying regulation of gene expression.

在pFOG405重组质粒中的大肠杆菌碱性磷酸酶基因(PhoA)的启动子及信号肽区的核苷酸顺序被测定并同Kikuchi等人在pYK190重组质粒中所报道的顺序进行了比较。结果指出,包含有PhoA基因启动子及信号肽的PvuⅡ-HpaⅠ片段对于调控外源基因的表达是足够的;启动子5’-端的二元对称结构(26bp)的缺失并不影响外源基因的表达水平。由于pYK190及pFOG405中PhoA基因启动子的上游区核苷酸顺序的不同源性,说明此上游区顺序与PhoA启动子调控下的外源基因表达无关。这一分析为我们利用PvuⅡ-HpaⅡ片段组建高效分泌表达载体及研究基因表达调控提供了有用信息。

A bifuctional anti-HBsAg-alkaline phosphatase fusion protein expression vector pHBFAP was constructed by attaching the PCR cloned E.coli alkaline phosphatase gene to the C-terminal of the anti-HBsAg Fd fragment gene. E.coli XL1-Blue was transformed with pHBFAP, and induced with IPTG. The HBsAg banding ability and the alkaline phosphatase catalytic activity were detected by using ELISA in the induced bacterial supernatant thus indicating that the anti-HBsAg-alkaline phosphatase bifunctional antibody was expressed...

A bifuctional anti-HBsAg-alkaline phosphatase fusion protein expression vector pHBFAP was constructed by attaching the PCR cloned E.coli alkaline phosphatase gene to the C-terminal of the anti-HBsAg Fd fragment gene. E.coli XL1-Blue was transformed with pHBFAP, and induced with IPTG. The HBsAg banding ability and the alkaline phosphatase catalytic activity were detected by using ELISA in the induced bacterial supernatant thus indicating that the anti-HBsAg-alkaline phosphatase bifunctional antibody was expressed successfully in E.coli.

为构建带有碱性磷酸酶活性的双功能基因工程抗体 ,用PCR方法克隆大肠杆菌碱性磷酸酶基因 ,通过酶切分析和DNA序列测定核实后 ,将其重组到抗乙肝表面抗原 (HBsAg)Fab段的Fd羧基端 ,构建重组融合蛋白表达载体pHBFAP ,转化大肠杆菌XL1 Blue ,经异丙基硫代 β D 半乳糖苷诱导表达后 ,采用ELISA法检测到培养上清中存在与HBsAg的结合活性和碱性磷酸酶的催化活性 ,显示抗HBsAg 碱性磷酸酶双功能抗体分子在大肠杆菌中获得了表达 .

A bifuctional anti-HBsAg-alkaline phosphatase fusion protein expression vector pHBFAP wasconstructed by attaching the PCR cloned E coli. alkaline phosphatase gene to the c-terminal of the anti-HBsAgFd fragment gene. E. coli XL1-Blue was transformed with pHBFAP, and induced with IPTG. The HBsAg band-ing ability and the alkaline phosphatase catalytic activity were detected using ELISA in the induced bacterial su-pernatnat thus indicating that the anti-HBsAg-alkaline phosphatase bifunctional antibody was expressed...

A bifuctional anti-HBsAg-alkaline phosphatase fusion protein expression vector pHBFAP wasconstructed by attaching the PCR cloned E coli. alkaline phosphatase gene to the c-terminal of the anti-HBsAgFd fragment gene. E. coli XL1-Blue was transformed with pHBFAP, and induced with IPTG. The HBsAg band-ing ability and the alkaline phosphatase catalytic activity were detected using ELISA in the induced bacterial su-pernatnat thus indicating that the anti-HBsAg-alkaline phosphatase bifunctional antibody was expressed success-fully in E. coli.

为构建带有碱性磷酸酶活性的双功能基因工程抗体,我们将已克隆的大肠杆菌碱性磷酸酶基因重组到抗HBsAg Fab段的Fd羧基端,构建了重组融合蛋白表达载体pHBFAP,转化大肠杆菌XLl-Blue,经IPTG诱导表达后,采用ELISA法检测到培养上清中存在与HBsAg的结合活性和碱性磷酸酶的催化活性,显示抗HBsAg-碱性磷酸酶双功能抗体分子在大肠杆菌中获得了表达.

 
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