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限制性内切酶谱分析
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  restriction endonuclease pattern analysis
     The cDNA fragment was amplified by RT PCR with specific primers. The purified RT PCR product was cloned in pGEM T Easy vector. The results of restriction endonuclease pattern analysis of the recombinant plasmid, DNA sequencing, and aligning of the putative amino acid sequence with hBD 1 and mBD 1 demonstrated that rat β defensin rBD 1 gene was cloned successfully.
     从大鼠肾脏组织提取RNA,采用RT-PCR技术扩增大鼠β-防御素rBD-1cDNA,并重组到pGEM-T Easy 载体,经限制性内切酶谱分析和DNA序列测定,证实所克隆的cDNA片段为大鼠β-防御素rBD-1 cDNA。
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  “限制性内切酶谱分析”译为未确定词的双语例句
     Results: The restrictive endonuclease mapping showed that the restrictive endonuclease sites of pBKcmv-MLCK were identical with the original design, the insert fragment of pET28b-MLCK is 405bp which is identical with the design.
     结果:重组质粒pBKcmv-MLCK限制性内切酶谱分析表明其酶切位点与原设计相符。 重组质粒pET28b-MLCK中插入片段大小为405 bp,与设计一致。
短句来源
     Here we report the identification of a novel β–defensin from camel tisses,camel β–defensin-1(caBD-1). Based on the conserved sequence of other cud chewer,the primer were designed. Total RNA was extracted from the tongue epithelia of camel and the cDNA encoding caml β–defensin (caBD-1) was amplified by the reverse transcription PCR(RT-PCR).
     我们利用其他反刍动物(牛和羊)cDNA的保守序列设计引物,从骆驼舌黏膜上皮组织中提取总RNA,采用RT-PCR技术扩增出caBD-1(camelβ-defensin-1)的cDNA,并重组到pBlueselect T载体,经限制性内切酶谱分析和DNA序列测定,证实所克隆的caBD-1的cDNA为β-防御素。
短句来源
     Methods PCR products of the chromosome DNA of the CWDMs were analysed by restriction zymogram.
     方法对伤寒沙门菌CWDMs染色体的特异性PCR扩增产物进行限制性内切酶谱分析
短句来源
     Then a subclone cL6. 4 was constructed by inserting the 6. 4 kb EcoR I fragment into pTZ19R vector. Restriction analysis of clone cL6. 4 was reported.
     用该片段构建了鸡的GnRH-I亚克隆cL6.4,并对其作了限制性内切酶谱分析.
短句来源
     Restriction Endonuclease Analysis of Leptospiral DNA from Different Serogroup and Serovar
     不同血清群型钩端螺旋体DNA的限制性内切酶谱分析
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  相似匹配句对
     Restriction enzyme pattern analysis of Mycobacteria genosome
     分枝杆菌属基因限制性内切酶谱分析
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     An analysis of restriction zymogram of the chromosome DNA of cell walldeficient mutants of S.ty phi .
     伤寒沙门菌CWDMs染色体的限制性内切酶谱分析
短句来源
     Restriction Endonuclease Analysis of Leptospiral DNA from Different Serogroup and Serovar
     不同血清群型钩端螺旋体DNA的限制性内切酶谱分析
短句来源
     The results also show that the relations of E.
     从POD酶谱分析,E.
短句来源
     Specificity of the Recombinant DNA pCX7 and Its Restriction Enzyme Map
     赖型钩端螺旋体重组DNApCX7的特异性鉴定和限制性内切酶谱分析
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Interleukin-2(IL-2) has a very broad spectrum of activities and plays an important role in immune regulation. The cDNA clones encoding human IL-2 were gained from human PBL cDNA library with a nonradioactive digoxigenin-probe prepared from mouse IL-2 cDNA clone. In order to increase the screening efficiency, we pretreated the library with a serieys of restriction enzymes which were not present within human IL-2 cDNA and plasmid vector and made the positive rate of human IL-2 clone raise 33 times as high as that...

Interleukin-2(IL-2) has a very broad spectrum of activities and plays an important role in immune regulation. The cDNA clones encoding human IL-2 were gained from human PBL cDNA library with a nonradioactive digoxigenin-probe prepared from mouse IL-2 cDNA clone. In order to increase the screening efficiency, we pretreated the library with a serieys of restriction enzymes which were not present within human IL-2 cDNA and plasmid vector and made the positive rate of human IL-2 clone raise 33 times as high as that of the primary library.By this "reverse selecting method" , two cDNA clones were isolted and identified by restriction mapping-The COS-7 cells were transfec-ted with the isolated cDNA clone(in pcD plasmid vector) and the resulting supernatants could effectively maintain in vitro growth of mouse IL-2 dependent line FI-2.

白细胞介素—2是免疫应答过程中由T细胞释放的一种淋巴因子,具有较广泛的生物学作用。本文报告采用负向选择增富法处理人外周血淋巴细胞cDNA文库,用一种新型非放射标记技术制成地高辛素-dUTP-鼠白细胞介素-2cDNA片段探针,从中筛选到2个阳性克隆,使阳性率提高33倍。经限制性内切酶谱分析,所获克隆与公布的人白细胞介素-2cDNA顺序相符。该cDNA转染CoS-7细胞后表达的重组人白细胞介素-2可维持小鼠白细胞介素-2依赖株FI-2生长。

α-Thalassemia major is a hereditary disease with a syndrome of hemolytic anemia, often caused bv the complete or partial deletion of a-globin genes. The authors isolated and purified the human α-globJn gene (a DNA fragment of 1.6kb) from a recombinant DNA of the plasmid PBR322, and labelled this fragment with α-32p-dCTP by nick translation as a probe for the detection of human α-globin gene. The genomic DNA of patients with Hb-H disease and hydrops fetalis is hybridized with this probe after the cleavage by...

α-Thalassemia major is a hereditary disease with a syndrome of hemolytic anemia, often caused bv the complete or partial deletion of a-globin genes. The authors isolated and purified the human α-globJn gene (a DNA fragment of 1.6kb) from a recombinant DNA of the plasmid PBR322, and labelled this fragment with α-32p-dCTP by nick translation as a probe for the detection of human α-globin gene. The genomic DNA of patients with Hb-H disease and hydrops fetalis is hybridized with this probe after the cleavage by various restriction enzymes. Hb-H adeficiency form samples produced 3 specific DNA fragments of 16.4, 4.5 and 3.8 kb in length when cut by HindⅢ, Hb-Hdisease samples produced 2 specific fragments of 16.4 and 4.5kb. Hb-H adeficicncv was 12.3 and 7.5 kb in length by BglⅡ, and Hb-H deficiency form and specific DNA fragments of 16 or 7.5 kb. The samples taken from fetus with hydrops fetalis produced no specific bands after the cleavage by various restriction enzymes when being hybridized with the α-globin gene probe.

α-地中海贫血是由于α-珠蛋白基因全部和部分缺失所致的一种遗传性溶血性贫血综合征。本文从重组质粒pHα_2中提取和纯化α_2片段(1.6kb),以α-~(32)p-dCTP标记做珠蛋白基因探针对Hb-H病人和Hb-Bart水肿综合征的血细胞DNA进行限制性内切酶谱分析。用HindⅢ和BglⅡ酶解Hb-H病人的非缺失型分别得16.4、4.5和3.8Kb三个特异片段和12.5、7.5二个片段。而Hb-H缺失型病人分别得到16.4、4.5和7.5或16Kb特异的α-基因片段。酶解Hb-Bart胎儿血DNA则没有α-基因特异片段。

Using a 341 bp GnRH-1 cDNA fragment as a probe, 10 positive clones wee selected from a 5×103 λEMBL3 chicken genomic library. An EcoR I fragment sized 6. 4 kb ho mologous to the probe was isolated from one of the positive clones. Then a subclone cL6. 4 was constructed by inserting the 6. 4 kb EcoR I fragment into pTZ19R vector. Restriction analysis of clone cL6. 4 was reported.

用鸡的GnRH-IcDNA为探针,从5×10~5的鸡λEMBL3基因文库中筛选到10个阳性克隆.从这些阳性克隆中分离出一段包含着GnRH-I信息,长度为6.4kb的EcoRI片段.用该片段构建了鸡的GnRH-I亚克隆cL6.4,并对其作了限制性内切酶谱分析.

 
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