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基因区
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  gene region
     No major effect of the CD28/CTLA4/ICOS gene region on susceptibility to primary sclerosing cholangitis
     CD28/CTLA4/ICOS基因区对原发性硬化性胆管炎的易感性没有主要影响
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     Use of PCR method for rapid detection of codon 550 mutation in HBV polymerase gene region of chronic hepatitis B patients treated by lamivudine
     用PCR方法快速检测用拉米夫定治疗的乙型肝炎病人中HBV P基因区codon 550的变异
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     Objective To design pSilencer3.1-H1hygro plasmid expressing short interfering RNAs(siRNA) that target HBV S gene region, and to evaluate inhibitory effect of this siRNA on HBV in vitro.
     目的以乙型肝炎病毒(HBV)S基因区为靶位,构建表达siRNA的质粒载体pSilencer3·1-H1hygro,体外观察siRNA抗HBV的效果。
短句来源
     for toxoplasmosis(TOXO) and neisseria gonorrhoeae(NG)by two gene region PCR,in every specimen.
     淋球菌 (NG)与弓形体 (TOXO)采用两个基因区 PCR。
短句来源
     A multiplex reverse transcription polymerase chain reaction(m-RT-PCR) was developed for the simultaneous detection of potato virus X, potato virus A and potato virus S. The primers for PVX (562bp) and PVS (182bp) fragments were designed based on the coat protein region and primers for PVA(255bp) based on the P1 gene region.
     根据病毒外壳蛋白区序列设计PVX、PVS特异性引物对 ,根据P1基因区序列设计PVA特异性引物对 ,应用三重RT PCR同步检测马铃薯X病毒 ,马铃薯A病毒及马铃薯S病毒 ,分别得到 5 62bp、 2 5 5bp、 1 82bp大小的扩增片段。
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  gene regions
     A comparative research on inhibition of hepatitis B virus replication by antisense oligonucleotides against various gene regions
     不同基因区反义寡核苷酸抑制乙型肝炎病毒基因表达的比较
短句来源
     ASONs designed against the S gene inhibited the secretion of HBsAg more effectively than those designed against C and pre C gene regions( P <0.05), while the latter inhibited HBeAg secretion more effectively than the former( P <0.01).
     反之,在C基因区设计的ASON对HBeAg的抑制作用又明显优于S和Pre-S2基因区的ASON(P<0.01)。
短句来源
     \ Methods\ The amplified fragment length polymorphism of 9 gene regions of the dysdtrophin gene in 10 DMD families was linkedly analyzed by multiplex PCR, PAGE and silver staining.
     ②方法 运用多重聚合酶链反应(PCR)、聚丙烯酰胺凝胶电泳银染法,对10 个DMD家系成员的抗肌营养不良蛋白基因内9个基因区进行了Am p-FLP连锁分析。
短句来源
     Genome of canine distemper virus(CDV) is approximately 16 kb. The genome contains six non-o- verlapping gene regions,organizes as 3′-N-P-M-F-H-L-5′,respectively codes six structural proteins. Nucle- ocapsid protein,Phosphoprotein,Matrix protein,Fusion protein,Attachment protein or Hemagglutinin protein and the large virus specific RNA directed RNA polymerase protein.
     犬瘟热病毒基因组长约16kb,包括6个非重叠基因区,按3′-5′端顺序依次为前导序列、N-P- M-F-H-L以及引导序列,分别编码核衣壳蛋白、磷蛋白、基质膜蛋白、融合蛋白、附着或血凝蛋白和大蛋白6种结构蛋白。
短句来源
  “基因区”译为未确定词的双语例句
     Of these 18 samples 13 were identified as HIV-1 CRF01AE(72.2%),and B'subtypes,BC recombinants and C subtype were identified in 1(5.6%),2(11.1%) and 1 samples(5.6%),respectively.
     基于gag基因区的序列分析结果表明:其中13份为CRF01 AE亚型(占72.2%),1份为B’亚型(占5.6%),2份为B’/C重组型(占11.1%),1份为C亚型(占5.6%),另有1例样本不确定。
短句来源
     Results The genetic distance respectively is 7.56% in tat gene between SC9 712 and UG274A, 9.59% and 9.94% in gag gene between SC9 712 and K31 and VI203, 13.86% in env gene between SC9 712 and MAL.
     结果发现SC9712在gag区、env区和tat区与国际标准D亚型毒株的基因距离最近,其中在gag区与V1203和K31的基因离散率分别为994%和959%,在tat基因区与UG274A的基因离散率只有756%,在env区与MAL的基因离散率是1386%。
短句来源
     Clinical Study on the Codon550 Variation of HBVP Gene of Chronic Hepatitis B Patients by Lamivudine
     拉米夫定治疗的慢性乙型肝炎患者HBVP基因区codon 550变异及临床研究
短句来源
     Results The positive rates of detection with primers deduced from 5′UTR, NS4 and NS5 were 92%, 62% and 59% respectively.
     结果HCV不同基因区(5′UTR、NS4、NS5b)引物的PCR扩增阳性率分别为92%、62%、59%。
短句来源
     A recombinant plasmid comprising adenovirus genome with rabies virus glycoprotein gene in E1 region was constructed by homologous recombination.
     利用大肠杆菌内质粒间同源重组的方法,将狂犬病病毒G基因插入腺病毒基因组的E1基因区,构建了带有狂犬病病毒G基因的重组腺病毒质粒。
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  gene region
Partial mitochondrial cytochrome b gene region (636 base pair) was sequenced to these samples and 22 haplotypes were found.
      
Screening for mutations has identified a population polymorphism in the 5'-upstream miR-130b gene region containing DNA elements for putative transcription factors.
      
Only p52 was covalently bound with the 28S rRNA gene region in lymphocytes exposed in UV light.
      
Several point substitutions in the ND6 gene region proved to be associated with particular transitions in the mtDNA control region; the association was characterized with the ? coefficient.
      
Site-specific endonucleases F-TflI, F-TflII, and F-TflIV have been revealed, which belong to the H-N-H family and are encoded by ORFs located in the tRNA gene region of bacteriophage T5.
      
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  gene regions
About 70% of all rDNA copies proved poorly accessible for endonucleases and arylazide, the accessibility being higher in their 18S and 5.8S rRNA gene regions than in the regions of the external transcribed spacers (ETSs) and the 28S rRNA gene.
      
Formalized description of the structural and functional organization of the regulatory gene regions is illustrated with examples.
      
Only 254 nucleotide sequences contained MIRs in coding regions, of which 45 MIR copies were unknown before, including 17 that occurred in translated gene regions.
      
With the example of yeast genes, context organization was compared for functional gene regions (promoter, 5"-UTR, 3"-UTR) and tested for association with the level of gene expression.
      
An internal DNA fragment (~2000 bp) homologous to the conserved regions of genes encoding latrophilin-like proteins (LLPs) was obtained by the PCR technique using degenerate primers to these gene regions.
      
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The organization of 5S rRNA genes(5S DNA) of silkworm Attacus ricini has been determined by means of Southern's method.The 5S DNA consists of hundreds of tandemly repeated units which are 260 base pairs in length.There is a single recognition site in each repeated unit for the restriction enzymes MboI and HhaI respectively.Both of these sites exist inside the structural gene region.The 5S DNA does not contain any recognition sites for restriction enzymes EcoRI,BamHI,PstI and BglII.The spacer region of Attacus...

The organization of 5S rRNA genes(5S DNA) of silkworm Attacus ricini has been determined by means of Southern's method.The 5S DNA consists of hundreds of tandemly repeated units which are 260 base pairs in length.There is a single recognition site in each repeated unit for the restriction enzymes MboI and HhaI respectively.Both of these sites exist inside the structural gene region.The 5S DNA does not contain any recognition sites for restriction enzymes EcoRI,BamHI,PstI and BglII.The spacer region of Attacus ricini 5S DNA is the smallest one among the eucaryotic 5S DNAs which have ever been investigated.Whereas,like all others,it has remarkable sequence heterogeneity which can be revealed by the Haelll and AluI digestion patterns.

用Southern方法研究了蓖麻蚕(Attacus ricini)5S核糖体RNA (5S rRNA)的基因在基因组中的组织情况。5S rRNA基因以顺向串联的多拷贝形式组成了5S DNA家族。家族的每个成员(5S DNA的重复单位)的大小为260bp(碱基对),即由120bp的基因区和140bp的空间区顺序所组成。在5S DNA的家族中没有限制性内切酶EcoRI、BamHI、PstI和BglⅡ的切点。限制性内切酶MboI、Hhal在每个重复单位中有一个切点,都是位于基因的顺序中。5S DNA重复单位的空间区顺序是不均一的,这一点可由限制性内切酶Hae Ⅲ和Alu Ⅰ切点的不规则分布而得以揭示。

The degrees of methylation of MS3 gene regions detectable with MS3 probe were found to be nearly the same in F9 genome and TDM1 genome. Nuclear transcripts of MS3 genes were detected not only in F9 cells but also in TDM1 cells. These results suggest that the failure of detection of MS3 RNA in the cytoplasm of differentiated cells may be mainly due to the defect of splicing the nuclear transcripts of MS3 genes or inability of transfer of the mature MS3 RNA from nucleus to cytoplasm.

MS_3 RNA是小鼠胚胎癌细胞特异RNA。为了阐明MS_3基因在未分化细胞中特异表达的机理,我们应该首先确定此特异表达是在哪一级水平上被调控的。Naveh-Many等(1981)指出积极进行转录的基因一般是甲基化程度不足的。由于MS_3基因有一个中等重复的家族,因此如果MS_3基因的调控主要在转录水平上进行,我们应该发现MS_3基因的甲基化程度在分化细胞及未分化细胞之间有明显差别,于是用测定甲基化程度的一组酶HpaⅡ和Msp1水介代表分化细胞的TDM_1及代表未分化细胞的F_9的高分子量DNA,然后用MS_3 cDNA作探针分析MS_3基因区的甲基化程度。在可用MS_3 cDNA探查到的范围内,MS_3基因区的甲基化程度在TDM_1及F_9细胞中未见明显不同。对这一结果可有四种不同解释:1.MS_3基因家族中除少数成员外其余绝大部分成员在未分化细胞中是不转录的,在分化细胞中则全部不转录:2.MS_3基因家族的绝大部分成员在未分化细胞以及在分化细胞中都是转录的;3.虽然MS_3基因家族中的绝大部分成员都是不转录的,但个别成员在未分化细胞以及分化细胞中都是转录的;4.由于MS_3 cDN...

MS_3 RNA是小鼠胚胎癌细胞特异RNA。为了阐明MS_3基因在未分化细胞中特异表达的机理,我们应该首先确定此特异表达是在哪一级水平上被调控的。Naveh-Many等(1981)指出积极进行转录的基因一般是甲基化程度不足的。由于MS_3基因有一个中等重复的家族,因此如果MS_3基因的调控主要在转录水平上进行,我们应该发现MS_3基因的甲基化程度在分化细胞及未分化细胞之间有明显差别,于是用测定甲基化程度的一组酶HpaⅡ和Msp1水介代表分化细胞的TDM_1及代表未分化细胞的F_9的高分子量DNA,然后用MS_3 cDNA作探针分析MS_3基因区的甲基化程度。在可用MS_3 cDNA探查到的范围内,MS_3基因区的甲基化程度在TDM_1及F_9细胞中未见明显不同。对这一结果可有四种不同解释:1.MS_3基因家族中除少数成员外其余绝大部分成员在未分化细胞中是不转录的,在分化细胞中则全部不转录:2.MS_3基因家族的绝大部分成员在未分化细胞以及在分化细胞中都是转录的;3.虽然MS_3基因家族中的绝大部分成员都是不转录的,但个别成员在未分化细胞以及分化细胞中都是转录的;4.由于MS_3 cDNA只能探查MS_3基因的分端区域,可能此区域的甲基化程度与MS_3基因的转录水平无关。为了区别这些可能性,我们检查了F_9细胞及TDM_1细胞中的核RNA,发现在这二种细胞核内都存?

Histones are a class of basic proteins that associate with each other and with nuclear DNA to form the nucleosome.To study the histone genes in plants,we have constructed a rice genomic library using a lambda vector,EMBLa.Two clones of histone H2 genes have been isolated from the library and their organizations have also been analysed with restriction enzymes.The clone λRH2-1 contains two HI genes separated by a 5.8kb spacer; the other,λRH2-2,has one large region (5.2 kb EcoRl-HindIII fragment) homologous to...

Histones are a class of basic proteins that associate with each other and with nuclear DNA to form the nucleosome.To study the histone genes in plants,we have constructed a rice genomic library using a lambda vector,EMBLa.Two clones of histone H2 genes have been isolated from the library and their organizations have also been analysed with restriction enzymes.The clone λRH2-1 contains two HI genes separated by a 5.8kb spacer; the other,λRH2-2,has one large region (5.2 kb EcoRl-HindIII fragment) homologous to H2 gene with distinct enzyme sites.After comparing with other studies on rice histone genes,we suggest that the histone H2 gene organizations in rice be similar to that in higher animals like chicken or human.

用噬菌斑原位杂交法,从一个水稻新品系的EMBL,基因文库中筛选到了两个组蛋白H,基因克隆,并制作了它们的限制性内切酶图谱,分析了它们的组织结构。其中λRH_3-1克隆包含2个组蛋白H_3基因区(分别为0.5kb和0.8kb的BamHI-EcoRI片段,两基因区距离5.8kb)。而λRH_3-2克隆只带有1个结构特异的H_3基因区(5.2kb的EcoRI-HindⅢ片段)。本文还就水稻组蛋白H_3基因在整个基因组中的组织结构特点进行了讨论。

 
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