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Most obviously, these plates are not designed for cryopreservation or to withstand the low temperatures encountered during liquid nitrogen cryopreservation, or both.
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| | After gradient centrifugation by using Urograffin-Ficoll mixture (d=1.097g/cm~3) most of the haemopoietic cells could be recovered in the mononuclear cell layer containin enriched granuroid progenitor cells (GFU-C), but the efficacy of separation of CFU-C was also dependant on the size of the centrifuge tube used. The isolated mononuclear cells after gradient centrifugation were cryopreserved in liquid nitrogen by two steps of cooling rates. The mortality of time from a few days to three months was in the r... | | 应用聚蔗糖——泛影葡胺分层液对人胎肝细胞作非连续密度梯度分离中,CFU-C的分离效率与所用离心管的大小有关。分离的胎肝细胞经液氮低温保存三个月后,其中58—88%的CFU—C可以得到回收。 | | 文摘来源 | | Two cryoprotective recipes, A: 10% glycerol+4% glucose, B: 15% DMSO (dimethl sulfoxide ) + 4 % glucose + 2 % citrate, were used in cropreservation of embryos of Chinese scallop,Chlamys farreri,by the aid of Programmed Cryobiological Controller. Using the freezing procedure,i. e. 1℃ /min to - 20℃, 20℃/min to - 80℃, then plunging the samples into liquid nitrogen, the scallop embryos (6d, veliger) survival rate can reach as high as 65. 1 % (recipe A) and 56. 5% (recipe B) after thawing in 45℃ sea wa- ter b... | | 选用两种低温保护剂配方,A;10%甘油+4%葡萄糖;B:15%DMSO(二甲 基亚铜)+4%葡萄糖+2%柠檬酸盐,利用微机控制生物降温仪,对栉孔扇贝胚胎 进行液氮低温保存。以1℃/min由室温降温至-20℃,接着以20℃/min降温至- 80℃,然后样品直接入液氮保存(-196℃),1周后,经45℃水浴解冻,栉孔扇贝胚 胎(发育 6d)的存活率达 65. 1%(A配方)和 56. 6%(B配方);而采用6℃/min降温 至-20℃,并以 20℃/min降温至-80℃,直接入液氮,胚胎解冻后的存活率分别为 39.0%(A配方)和27.9%(B配方)。选用发育24h的栉孔扇贝胚胎进行液氮保存 实验,解冻后,胚胎基本死亡。 | | 文摘来源 | | For the purpose of opening up the future of more widespread application of cord blood,and also for the purpose of setting up the, bank of hemopoietic stem sells in cord blood,an effective method of cryopreserving the cord blood was approached, indicating, that it was appropriate that the time of cryopreserving the hemopoietic stem cells in cord blood at 4℃ should be 2-3 days (the storage of hemopoietic stem cells in cord blood with liquid nitrogen was an effective method of cryopreserving the hemopoietic st... | | 为使脐血有更广阔的应用前景及建立脐血造血干细胞(CBSC)库,笔者探讨了脐血低温保存的有效方法。结果显示:CBSC 4℃低温保存以2~3d为宜;液氮低温保存是一种有效的CBSC保存方法,回收率高,较长时间保存,回收率不受影响;-80℃冰箱保存法具有与液氮相同的低温保存效果;且简单、经济、方便和实用,无需程序降温仪,便于大规模开展应用和建立CBSC库;脐全血保存可以避免分离过程CBSC的丢失,保存效果不受影响。 | | 文摘来源 | |   | | << 更多相关文摘 |
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