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激活的骨髓
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  activated bone marrow
     Experimental study on purging effect of IL-2 activated bone marrow in combination with IFN-αon K562 leukemic cells
     IFN-α联合IL-2激活的骨髓净化K562细胞的实验研究
短句来源
     The purging effect of IL-1 and IL-2 activated bone marrow on leukemic cells
     IL-1与IL-2联合激活的骨髓对白血病细胞的净化作用
短句来源
     Objective To study the anti tumor mechanism of IL 2 activated bone marrow (ABM).
     目的 探讨白细胞介素 2 (IL 2 )激活的骨髓 (ABM )抗肿瘤作用的免疫学机理。
短句来源
     Purging Effect of IFN-α and IL-2 Activated Bone Marrow Cells on Leukemi a Cells and Preliminary Studies on the Cytotoxicity Mechanisms
     IFN-α联合IL-2激活的骨髓对白血病细胞的净化作用及其机制初探
短句来源
     IL— 2 activated bone marrow (AMB) cells have purging effect on leukemic cells, and GVL can be induced by IL —2 after ABMT.
     IL-—2激活的骨髓有净化白血病细胞的作用,移植后应用IL—2能诱导GVL。
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  bone marrow activated
     Study of bone marrow activated with CD_3 monoclonal antibody and rhinterleukin-2 for killing and purging leukemia cells in vitro
     CD_3 McAb联合rhIL-2激活的骨髓对白血病细胞杀伤净化作用的研究
短句来源
     Objective To study the role of bone marrow activated in vitro with CD 3 monoclonal antibody(CD 3McAb) and rhinterleukin 2(rhIL 2)for killing and purging leukemia cells.
     目的 探讨CD3单克隆抗体 (CD3McAb)联合重组人白细胞介素 2 (rhIL 2 )激活的骨髓对白血病细胞的杀伤及净化作用。
短句来源
     Results The significant roles of killing and purging K562 cells and HL 60 cells through bone marrow activated CD 3McAb group( P <0.05 or P <0.01).
     结果 CD3McAb与rhIL 2联合激活的骨髓对白血病细胞株K5 6 2、HL 6 0均有明显的杀伤净化作用 ,且优于rhIL 2或CD3McAb单用组 (P <0 .0 5或P <0 .0 1)。
短句来源
     Objective:In order to study the enhancing effect of anti leukmia of bone marrow activated by rIL 2.Methods:We used anti CD 3 monoclonal antibody and rIL 2 jointly to activate bone marrow in vitro and detected the killing activity of activated bone marrow (ABM) under various circumstances by method of MTT.
     目的:为了研究增强rIL- 2 激活的骨髓细胞(激活骨髓ABM)的抗白血病效应。 方法:应用抗CD3 单抗与rIL- 2 联合作用体外激活骨髓细胞,采用MTT比色分析法测定不同条件下ABM杀伤肿瘤细胞活性。
短句来源
     Conclusion The action of killing and purging leukemia cells by using bone marrow activated with rhIL 2 can be strengthened when combined with CD 3McAb. This study may provide a theoretical and experimental basis for auto BMT's action of purging in vitro .
     结论 CD3McAb能增强rhIL 2激活的骨髓对白血病细胞的杀伤及净化作用
短句来源
  “激活的骨髓”译为未确定词的双语例句
     The levels of CFU GM of ABM had no significant effect when using CD 3McAb or rhIL 2. The expressions of CD3, CD8, CD19, CD25, CD38 and CD56 on the surface of ABM with CD 3McAb and rhIL 2 were significantly promoted when compared to pre culture of any single group.
     rhIL 2和 (或 )CD3McAb对激活骨髓的CFU GM水平无明显影响。 CD3McAb +rhIL 2激活的骨髓 ,与活化前及rhIL 2或CD3McAb组相比 ,CD3+ 、CD8+ 、CD1 9+ 、CD2 5+ 、CD38+ 、CD56+ 细胞显著升高。
短句来源
     In that context,efforts were made to generate natural killer (NK) cells from bone marrow byincubating with interleukin-2 (IL-2) in vitro, known as IL-2 activated bonemarrow (IL-2 ABM).
     针对上述两点,有人将骨髓置于IL-2环境中孵育以诱生并活化骨髓来源的NK细胞,称之为IL-2激活的骨髓(IL-2 ABM)。
短句来源
     ANTITUMOR EFFECT OF rIL-2-ACTIVATED BONE MARROW CELLS——PURGING RESIDUAL LEUKEMIC CELLS IN ABMT
     rIL-2激活的骨髓细胞抗瘤活性——白血病自体骨髓移植净化
短句来源
     Inhibition on the clone formation of K562 leukamia line and effects on CFu-GM of normal myeloid granulocytes and monocytes were studied with myeloid LAK cells from 10 cases of AML and 5 cases of remission stage, which were induced and activated by IL-2 in intro.
     采用体外半固体培养技术,研究了10例急性髓细胞白血病(AML)患者和其中5例缓解期患者经IL-2体外诱导激活的骨髓LAK细胞对K_(562)白血病细胞系集落形成的抑制作用,以及对正常骨髓粒单系造血祖细胞集落(CFu-GM)的效应。
短句来源
     There was no obvious difference in CFU GM,BFU E formation rate between ABM that was activated by marrow cell for 3~5 days amd the bone marrow cell that was not activated but cultured for same time.
     体外激活 3~ 5 d的 ABM与同期培养的未激活的骨髓细胞相比 BFU- E、CFU- GM形成率差异无显著性 (P>0 .0 5 ) ,LAK细胞则不具有造血祖细胞活性。
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  activated bone marrow
CD4+CD25+ T regulatory cells induced by LPS-activated bone marrow dendritic cells suppress experimental autoimmune uveoretinitis
      
The potential usefulness of interleukin-2 activated bone marrow cells as an active therapeutic tool against cytomegalovirus infe
      
In the present study, we report that cytokine-activated bone marrow NS cells elaborated a soluble factor with sup pressive activities.
      
  bone marrow activated
The results showed that the cytotoxicity of MNCs from bone marrow activated by IL-2 on tumor cell line K562 was increased obviously and the number of CD25+ and CD70+ positive cells in bone marrow MNCs was higher than before activation.
      


Allogeneic bone marrow transplantations(AlloBMT)were performed in the lethal irradiated adult mice.The live time in the mice received lymphokine-activated bone marrow cells(LABMC)was much longer than thatof the controlled mice (P<0.001).Cancer transplanta tion and one way MLC demonstrated the success of AlloBMT. The results showed that LABMC permited long term engraftment of allogeneic marrow while preventing lethal GVHD.

以致死性剂量照射的成年小鼠为受鼠进行同种异体骨髓移植(Allo-BMT),实验组小鼠观察期间平均存活天数较对照组明显延长(P<0.001),应用肿瘤移植及单向混合淋巴细胞培养(MLC)二项指标证实Allo-BMT的部分植活。实验结果表明,淋巴因子激活的骨髓细胞(LABMC)可有效预防致死性移植物抗宿主病(GVHD),且不影响供髓植活。

Antitumor effect and hematopoietic function of non-separated bone marrow (nBM) cells activated with recombinant interleukin 2 (rIL-2) were studied. The nBM cells activated with rIL-2 for 24 hours were cytotoxic against both NK-sensitive K562 and NK-resistant Raji cells. Within a limited time, the longer the bone marrow cells activated, the higher the antitumor effect appeared and a climax was reached on the fourth day. There was no difference between nBM and separated BM cells activated with rIL-2 in their antitumor...

Antitumor effect and hematopoietic function of non-separated bone marrow (nBM) cells activated with recombinant interleukin 2 (rIL-2) were studied. The nBM cells activated with rIL-2 for 24 hours were cytotoxic against both NK-sensitive K562 and NK-resistant Raji cells. Within a limited time, the longer the bone marrow cells activated, the higher the antitumor effect appeared and a climax was reached on the fourth day. There was no difference between nBM and separated BM cells activated with rIL-2 in their antitumor effect. Leukemic stem cells' ability to form CFU-L decreased progressively in 5 acute myelogenous leukemia patients, along with the prolongation of time of activation of BM cells with rIL?. The nBM cells7 ability to form CFU-GM showed no difference in rIL-2 and non-rIL-2 culture medium. In 5 patients with leukemia treated by autologous bone marrow transplantation (ABMT), the nBM cells were purged with rIL-2 for 48-72 hours in vitro and hematopoietic reconstruction was not affected post-ABMT.

用rIL-2激活未分离的骨髓外胞(bone marrow cell,BMC)观察其抗瘤活性和造血功能。经rIL-2激活24h后的未分离BMC即表现对NK细胞敏感的K562和NK细胞不敏感的Raji细胞均有杀伤活性;在一定时间内,随激活时间延长抗瘤活性增强,在第4天达高峰。rIL-2激活的未分离BMC与分离BMC二者抗瘤活性无差异。5例急性非淋巴细胞白血病病人骨髓经rIL-2激活后,白血病祖细胞CFU-L生成能力下降;经rIL-2激活的骨髓细胞生成CFU-GM能力无下降。根据我们的研究结果提出,rIL-2激活未分离BMC可用于白血病ABMT中残留白血病细胞净化,其方法简便、实用。

Human bone marrow cells were incubated with IL-2 for 1 or 3 days to generate activated bone marrow(ABM)cells.3H-TdR release assay was used for tumoricidal activity appraisal.The results showed that the cytotoxicity of ABM cells against tumor cell line AGZY-83a and CEM was remarkable.The cytotoxicity of bone marrow after IL-2 activation for 3 days was much stronger than that of 1 day activation. Fresh bone marrow(FBM) alone or IL-2 alone had no influence on the anti-tumor activity.FBM contaminated with CEM cells(50/1)and...

Human bone marrow cells were incubated with IL-2 for 1 or 3 days to generate activated bone marrow(ABM)cells.3H-TdR release assay was used for tumoricidal activity appraisal.The results showed that the cytotoxicity of ABM cells against tumor cell line AGZY-83a and CEM was remarkable.The cytotoxicity of bone marrow after IL-2 activation for 3 days was much stronger than that of 1 day activation. Fresh bone marrow(FBM) alone or IL-2 alone had no influence on the anti-tumor activity.FBM contaminated with CEM cells(50/1)and treated with IL-2 showed significant ability to purge autologous CEM cells.Colonogenic potential(CFU-GM) of ABM was not impaired compared with FBM.This study suggested that bone marrow can be activated with IL-2 to acquire the ability to eliminate contaminating tumor cells without affecting its progenitor cell function in vitro.

应用国产白介素2(IL-2)与骨髓细胞共同孵育1或3天,产生激活骨髓(分别记为ABM1或ABM3)。用3H-TdR释放法测定,表明ABM对肿瘤细胞株AGZY-83a和CEM具有明显杀伤活性。ABM3抗瘤活性明显高于ABM1,而新鲜骨髓或单用IL-2不具有明显抗肿瘤活性。ABM可以净化自身骨髓中的CEM细胞(效/靶为50/1),而ABM与对照组骨髓比较粒单集落形成率无统计学差别,提示ABM仍然保持其造血活性。本文结果初步提示ABM可用于净化骨髓中肿瘤细胞之目的。

 
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