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细胞增殖状态
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  cell proliferative activity
     Expression of P~(53) Protein in Laryngeal Cancer and its Relationship to Cell Proliferative Activity and Prognosis
     P~(53)蛋白在喉癌中表达与细胞增殖状态及预后的关系
短句来源
     Expression of p53 protein in ovarian cancer and its relationship to cell proliferative activity and prognosis
     卵巢癌p53蛋白表达与细胞增殖状态及预后的关系
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     Methods Using immunohistochemistry techniques, the relation between expression of mp53 protein, placental glutathione S transferase (GST P) and the cell proliferative activity was investigated in rat hepatic preneoplastic lesions induced by DEN and the control liver.
     方法 用免疫组化方法 ,观察二乙基亚硝胺( DEN)诱发大鼠肝癌前病变组织及对照组肝组织中突变型 p53( mp53)蛋白、胎盘型谷胱甘肽 S转移酶 ( GST- P)的表达及其与细胞增殖状态的关系。
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  “细胞增殖状态”译为未确定词的双语例句
     Methods: Primarily cultured cells were treated with different concentrations of CXB(0,2.5×10~(-6),5×10~(-6),1×10~(-5),2×10~(-5),3×10~(-5),4×10~(-5),5×10~(-5)mol/L) and the proliferative status was evaluated by BrdU assay.
     方法:原代培养多囊肾囊肿衬里上皮细胞,以不同浓度CXB(0、2.5×10-6、5×10-6、1×10-5、2×10-5、3×10-5、4×10-5、5×10-5mol/L)处理细胞,采用BrdU法检测细胞增殖状态
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     The study on the expression of h-met,c-myc and P 16 and cellular proliferative state in hepatic carcinoma
     肝癌h-met,c-myc,P~(16)蛋白表达与细胞增殖状态的研究
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     Methods Stat3 AS-ON,5-FU,or Stat3 AS-ON+5-FU was added into culture media. The expression of Stat3,p-Stat3,Cyclin D1 and bcl-xL was detected by Western blot. The cell proliferation was assayed by MTT.
     方法 应用Stat3AS ON与 5 FU处理结肠癌细胞HCT116,Westernblot检测Stat3、p Stat3、CyclinD1与bcl xL表达 ,MTT法检测细胞增殖状态 ,流式细胞技术检测细胞周期与凋亡。
短句来源
     METHODS: Human gastric cancer cell line BGC823 was treated with Stat5 AS-ON and 5-FU, respectively, or in combination. The ex- pression of Stat5, p-Stat5, cyclin D1 and Bcl-xL in the cells were detected by Western blot, and the cell cycle and apoptosis were detected by flow cytometry.
     方法:Stat5AS-ON、5-FU单用或联用处理胃癌细胞BGC823,Westernblot检测Stat5、p-Stat5、cyclinD1与Bcl-xL表达,MTT法检测细胞增殖状态,流式细胞技术检测细胞周期与凋亡.
短句来源
     (2) Ang Ⅱ(1× 10-7 mol.L-1 and 1 ×10-8mol . L-1) and angiotensin receptor type I (AT1) antagonist (losartan) were incubated with cultured TM cells. The cellular proliferation was measured by 3H-thymidine(3H-TdR) incorporation assay and collagen synthesis was indirectly professed though detects the hydroxyproline of medium with chemistry methods.
     (2)Aug Ⅱ(1×10-7mol·L-1及 1×10-8mol·L-1)以及其Ⅰ型受体(angiotensin receptor type I,AT1)拮抗剂孵育TM细胞,采用检测3H-TdR掺入率的方法了解细胞增殖状态,并用化学方法检测培养液中羟脯氨酸含量,间接推导胶原含量。
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     BrdU was used to show cell stage in division.
     BrdU掺入实验检测细胞增殖状态 ;
短句来源
     BrdU was used to show cell stage in division.
     BrdU掺入实验检测细胞增殖状态
短句来源
     The cellular proliferation was detected using flow cytometry.
     流式细胞仪检测细胞增殖状态;
短句来源
     Objective:To study the cellular proliferating activity of the endolymphatic sac.
     目的 :了解大鼠内淋巴囊的细胞增殖状态
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     The ALP granul es in the cells stained thicker and th e cells' proliferation cycle shortened.
     细胞增殖周期缩短。
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  cell proliferative activity
These are differences in cell proliferative activity, as well as changes in compartmentalization and migration of cells with abnormal karyotypes.
      
The overexpressed p53 could enhance cell proliferative activity in pretreated NPCs represented by increasing of MI, but showed no effect on neoplastic cell apoptosis.
      
Investigation of the relationship between cell proliferative activity and invasion, metastasis and prognosis of gastric cancer
      
Meanwhile, antisense ODN could significantly antagonize the FSH-promoted cell proliferative activity (P>amp;lt;0.01).
      
The gene encoding a chicken cytokine with T-cell proliferative activity was cloned, sequenced, and mapped.
      
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The effects of hyperthermia on murine marrow hemopoietic stem (progenitor) cells at different hierarchy of differentiation (CFU-S_1,CFU-S_2 and GM-CFU) in vitro were studied. It was found that all hemopoietic cells studied were thermotolerant when the bone marrow cells were heated up to 42℃ for 30 minutes. However, when temperature was over 43℃ for 30 minutes, the growth of GM-CFU and CFU-S_2 was progressively reduced (P<0.01), but no influence to the growth of CFU-S_1 was ob- served. These results suggest that...

The effects of hyperthermia on murine marrow hemopoietic stem (progenitor) cells at different hierarchy of differentiation (CFU-S_1,CFU-S_2 and GM-CFU) in vitro were studied. It was found that all hemopoietic cells studied were thermotolerant when the bone marrow cells were heated up to 42℃ for 30 minutes. However, when temperature was over 43℃ for 30 minutes, the growth of GM-CFU and CFU-S_2 was progressively reduced (P<0.01), but no influence to the growth of CFU-S_1 was ob- served. These results suggest that the effectiveness of hyperthermia on hemopoietic cells may be related to the proliferation state of cells, and that hyperthermy at 42℃ could be used clinically without hemopoietic damage.

随着热生物学的迅速发展,高热治疗肿瘤已引起广泛注意。本文测定了小鼠各年龄结构造血细胞对不同高热的反应。发现温度在≤42℃处理半小时,各种造血细胞对热能够耐受,当温度超过43℃时,CFU-S_2及GM-CFU的生长进行性下降,而CFU-S_1则影响较少。提示热疗法的效应与细胞增殖状态密切相关,临床应用,如果热疗在42℃时对造血系无明显影响。

T2 toxin is one of the most toxic member among trichotheccnes (Ts)family produced by fungi.It is responsible to intoxication found in human being and animals following ingestion of mouldy grain,and alimentary toxic aleukia (ATA)is the common but important problem in intoxication.The follows are some events in hcmotological toxicology of this toxin.Under a single dose (2.3mg/ kg ip,equal to LD30),T2 toxin decreased mice peripheral WBC count and a similar decline of circulating lymphocyte (L)also happened,but...

T2 toxin is one of the most toxic member among trichotheccnes (Ts)family produced by fungi.It is responsible to intoxication found in human being and animals following ingestion of mouldy grain,and alimentary toxic aleukia (ATA)is the common but important problem in intoxication.The follows are some events in hcmotological toxicology of this toxin.Under a single dose (2.3mg/ kg ip,equal to LD30),T2 toxin decreased mice peripheral WBC count and a similar decline of circulating lymphocyte (L)also happened,but an earlier transient increase of both WBC and L at 3 h following injection was observable.The nadir of WBC was 38% on d 1 and recovered to normal by d 7.The transient increase of polymorphonuclear neutrophil count maintained for 6 h then decreased gradually to its nadir on d 3 and returned to normal level by d 7.RBC and pt were uneffectcd.By determining CFU-S,CFU-GM,CPU-E and BFU-E of femoral bone marrow cells in mice,there were no killing effect for CFU-S during 0.5- 24 h after exposure to the toxin,but a maximal depression ofCFU-GM,CFU-E and BFU-E all were happened at 2 h,with a survival fraction by 27%,48% and 57% respectively.At 0.5 h following administration,CFU-GM decreased about 42% of its control,but CFU-E and BFU-E were 10- 20% lower only.So,the sensitivity of these three committed progenitor cells to T2 toxin was in the order CFU-GM>CFU-E>BFU-E.The recovery of CPU-GM was rapidly and with a rebound value 2-3 times higher than control at d 2 and d 3,and reached normal level by d 5.CFU-E and BFU-E regenerated gradually and recovered by d 3.By detecting at 2 h after intoxication,the dose-response curves for CFU-GM,CFU-E and BFU-E were all appeared the characteristics of exponential plateau pattern.The sensitivity of hemopoietic stem and progenitor cells to T2 toxin in concerning with the character of each cell population and its proliferative manner was discussed.

T2毒素2.3 mg/kg(相当LD_(30))单次ip后测定小鼠外周血象,BMNC及骨髓CFUs、CFU—GM、CFU—E和BFU—E的改变以了解T2毒素血液毒理的特征.外周血中WBC总数明显减少,RBC和pt无变化.T2毒素虽不影响CFUs的数量,但对CFU—GM、CFU—E和BFU—E均有杀伤作用,中毒后2 h三种细胞存活率达最低值分别为正常的27%、48%和57%,恢复速度甚快,5 d内全部正常.三种细胞的T2毒素剂量活存曲线均为指数坪型.分析了各类造血干细胞对T2毒素敏感性不同的原因可能与各种细胞固有特性和细胞增殖状态有关。

Hybridoma cells (SRBC-CI) secreting anti-B antibody were cultured in macroca-rrier-bioreactor (Bellco) system. Optimal conditions or setpoints for the growth of SRBC-CI cells and antibody production were initially determined by conventional cell culture methods. Over the course of 52-day running, culture media were harvested periodically and the cumulative amount was 23.3 liters. The HA titer ranged from 1:512 (8 liters) to 1:64 (6.4 liters). The product was tested extensively and found to be as good as or even...

Hybridoma cells (SRBC-CI) secreting anti-B antibody were cultured in macroca-rrier-bioreactor (Bellco) system. Optimal conditions or setpoints for the growth of SRBC-CI cells and antibody production were initially determined by conventional cell culture methods. Over the course of 52-day running, culture media were harvested periodically and the cumulative amount was 23.3 liters. The HA titer ranged from 1:512 (8 liters) to 1:64 (6.4 liters). The product was tested extensively and found to be as good as or even better than human polyclonal ABO reagents.

应用体外细胞大量繁殖系统生产抗人B型红细胞单克隆抗体,结合细胞生长和分泌抗体的特性,设置离线和在线参数等,对细胞增殖状态和生产抗体进行连续控制。一次运转52天,共收获含抗体培液23.25L,血凝效价1:64~1:512,为批量生产以单克隆抗体作为人血型定型试剂提供了条件。

 
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