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系膜细胞株
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  mesangial cell line
     Methods SV40MES13 mesangial cell line was used as cell model and the total activity of HKs was measured by using standard G 6 PDH coupled assay.
     方法 以SV40MES13系膜细胞株为细胞模型 ,使用 6 磷酸葡萄糖脱氢酶偶联比色法检测HKs活性 ;
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  “系膜细胞株”译为未确定词的双语例句
     METHODS: Cultured 1097 rat glomerular mesangial cells were divided into 8 groups: control, 10~(-9)M insulin, 10~(-8)M insulin, 10~(-6)M insulin, high glucose, mannitol, high glucose plus 10~(-6)M insulin, high glucose plus 10~(-9)M insulin.
     方法:将培养的大鼠1097系膜细胞株分为8组,正常对照组,生理浓度胰岛素组(10~(-9)M),低浓度胰岛素组(10~(-8)M),高浓度胰岛素组(10~(-6)M),高糖组(30mM),甘露醇组,高糖加高浓度胰岛素组,高糖加生理浓度胰岛素组。
短句来源
     Firstly the injury of AGEs and hyperglycation to Rat Glomerular Mesangial Cell(RGMC) and Mouse Kidney Cell(MKC); Secondly,the damage effect of glucose fluctuation on RGMC and MKC;
     一:糖基化终产物和高糖对大鼠肾系膜细胞株(Rat Glomerular Mesangial Cell,RGMC)及小鼠原代肾细胞(Mouse Kidney Cell,MKC)的联合损伤作用;
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     Materials and MethodsMesangial cells were cultured in the MEM culture medium in which contains the serum of fetal cattle at a concentration of 10% .
     材料与方法用含10%胎牛血清的MEM培养基培养SD大鼠肾系膜细胞株,亚传代3~5代细胞用于实验。
短句来源
     Methods:Cultured 1097 rat glomerular mesangial cells were divided into 8 groups:High glucose-treated group; Mannitol-treated group;
     方法:将培养的大鼠1097系膜细胞株分为8组:正常对照组,高糖组,甘露醇组,不同浓度胰岛素组,高糖加不同浓度胰岛素组。
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     Methods: Rat mesangial cells were cultured in vitro, the expression of angiotensin Ⅱ type 1 receptor (AT1) mRNA was measured by semi-quantitative reverse tanscription polymerase chain response(RT-PCR) after the cells were incubated with different concentration of CsA for 24 hours.
     方法 :体外培养大鼠系膜细胞株 ,用RT -PCR方法测定不同浓度环孢素 (0 ,2 5 0 ,5 0 0 ,10 0 0ng/ml)作用 2 4h对细胞表达血管紧张素 1型受体 (AT1)mRNA的影响 ;
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  相似匹配句对
     Participants B16F10 cell strain.
     研究对象 B16F10细胞
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     pAdeasy 1,293 cell strain;
     pAdeasy-1,293细胞;
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     Cell proliferation was assessed by MTT;
     MTT法测定系膜细胞增殖;
短句来源
     STIMULATION OF MESANGIAL CELLPROLIFERATION BY METHYLPREDNISONE
     甲基强的松龙刺激培养的系膜细胞增殖
短句来源
     ESTABLISHMENT OF GLOMERUAL MESANGIAL CELL LINES AND EFFECT FACTORS ON ITS GROWTH
     肾小球系膜细胞的建立及影响其生长的因素
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  mesangial cell line
This study presents a human stable immortalized mesangial cell line, designated IP15.
      
Figure 1 shows a photomicrograph of the mesangial cell line.
      


Mesangial

利用从SV-40病毒转基因小鼠肾脏分离得到的系膜细胞株(SV-40MC),观察了大黄素对SV-40和MC生长、增殖细胞核抗原(PCNA)及原癌基因c-mycmRNA表达的影响,大黄素可明显抑制SV-40MC3H-TdR掺入且与剂量呈正相关。经大黄素(5μg/ml)作用的SV-40MC,PCNA阴性细胞数明显高于对照(28%vs。5.5%、p<0.001)大黄素同样抑制SV-40MC,c-mycmRNA表达,且不受蛋白合成抑制剂放线菌酮的影响。结果表明:大黄素对异常增殖状态的系膜细胞生长也有明确抑制作用,细胞分裂周期受抑制、此过程与细胞周期基因c-myc表达受抑有关。

Objective To evaluate the contributions and regulatory charateristics of phorbal esters (PDD and PMA) and functional consequences in inducing the activities of hexokinases(HKs) and net glucose(Glc) utilization. Methods The total HKs activity and its isoforms were measured by a standard G6PDH-coupled assay and zonal electrophoresis in situ detection via a G6PDH-coupled reaction seperately.The net Glc utilization was measured indirectly as Glc disapperance from the medium using the standaul Glc oxidase-coupled...

Objective To evaluate the contributions and regulatory charateristics of phorbal esters (PDD and PMA) and functional consequences in inducing the activities of hexokinases(HKs) and net glucose(Glc) utilization. Methods The total HKs activity and its isoforms were measured by a standard G6PDH-coupled assay and zonal electrophoresis in situ detection via a G6PDH-coupled reaction seperately.The net Glc utilization was measured indirectly as Glc disapperance from the medium using the standaul Glc oxidase-coupled reaction. Results The levels of basal HKs activities in both SV40MES13 cells and primary cultured rat mesangial cells(MCs) were similarl (27±3 ) U/g protein vs (26±1 ) U/g protein] and HK Ⅱ was mainly isoform in Mcs. The phorbal esters (PMA and PDD) induced HKs activity in time-and dose-dependent manner and increased net Glc utilization by SV40MES13 cells. Conclusion SV40MES13 cells pmvide the useful cell culture models for the study on the Glc metabolism of mesangial cells. HK Ⅱexpression in MCs suggested that the possibility of hormone-regulated renal contributions to Glc homeostasis.This is a challenge for the traditional dogma that regauled renal as insulin-insensitive with respect to Glc metabolism. Increased HKs activity by PMA is accompanied with corresponding increase in net Glc utilization. These studies will provide a new theoretical basis for further research on the abnormality and regulation of saccharemetabolism in DN mesangial cells.

目的探讨糖尿病相关因素──蛋白激酶C(PKC)对己糖激酶(hexokinases,HKs)的特异性调节以及与细胞对葡萄糖净利用的相关性,并探讨HKs在糖尿病肾病中的作用。方法SV40转基因鼠肾小球系膜细胞株(SV40MES13)作为细胞模型,分别使用6-磷酸葡萄糖脱氢酶耦联比色法和醋酸纤维膜区带电泳、原位底物染色法检测HKs总活性及同工酶活性,用Trander氧化法检测细胞对葡萄糖的净利用。结果SV40MES13细胞株和原代培养的鼠系膜细胞具有相似的HK活性,且主要表达HKⅡ同工酶。PKC激活剂-佛波酯(PMA、PDD)以时间和剂量依赖形式特异地诱导了系膜细胞HKs的活性,并增加了细胞对葡萄糖的净利用。结论系膜细胞主要表达HKⅡ,提示胰岛素参与了系膜细胞葡萄糖的代谢过程;PKC使HK活性上调,增加了细胞对葡萄糖的净利用,提示细胞内葡萄糖流量与HKs活性呈正相关。这将为进一步探讨糖尿病肾病的发病机制提供依据。

Objective: Our aim was to study the concentrations and regulatory characteristics of heparin binding epidermal growth factor like factor (HB EGF) and its functional consequences in inducing the activities of hexokinases (HKs) and net Glc utilization. Methods: The SV40MES13 cell line was used as cell model for this experiment to measure the total activity of Hks by using a standard G6PDH coupled assay. The net Glc utilization was measured indirectly as Glc disappearance from the medium using the standard...

Objective: Our aim was to study the concentrations and regulatory characteristics of heparin binding epidermal growth factor like factor (HB EGF) and its functional consequences in inducing the activities of hexokinases (HKs) and net Glc utilization. Methods: The SV40MES13 cell line was used as cell model for this experiment to measure the total activity of Hks by using a standard G6PDH coupled assay. The net Glc utilization was measured indirectly as Glc disappearance from the medium using the standard Glc oxidase coupled reaction(Trinder). Results: The HB EGF induced HKs activity in time and dose dependent manners and increased the net Glc utilization by SV40MES13 cells. Conclusion: The HB EGF plays an important role in pathogenesis of diabetic nephropathy by inducing total HKs activity specifically and increases the net Glc utilization in mesangial cells.

目的 :探讨肝素结合性表皮生长因子 (heparin bindingepidermalgrowthfactor likegrowthfactor,HB EGF)对肾系膜细胞己糖激酶 (hexokinases,HKs)的调节及其对葡萄糖净利用的影响。方法 :以SV40MES13系膜细胞株作为细胞模型 ,用 6 磷酸葡萄糖脱氢酶偶联比色法检测系膜细胞HKs活性 ,并利用Trander氧化法检测系膜细胞葡萄糖的净利用。结果 :HB EGF以时间和剂量依赖形式诱导了系膜细胞HKs活性 ,并增加了系膜细胞对葡萄糖的净利用。结论 :HB EGF通过增加HKs活性 ,使系膜细胞内葡萄糖流量增加 ,参与了糖尿病肾病的发生和发展。

 
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