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体外相互作用
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  interaction in vitro
     Expression of Amyloid Precursor Protein and LRP6 in E.coli and Interaction in vitro
     淀粉样前体蛋白和LRP6在大肠杆菌中的表达及其体外相互作用
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  “体外相互作用”译为未确定词的双语例句
     Prokaryotic Expression of Troponin I2 and Its in vitro Interaction with ERRα1
     肌钙蛋白I2的原核表达及与ERRα1的体外相互作用
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     The molecular interaction between P53 and telomeric repeat binding protein 1 in vitro
     P53与端粒重复序列结合蛋白质1的体外相互作用
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     Bacteria expression plasmids expressing GST-E6 and GST-E7 were generated and GST fusion proteins purified. The purified GST-E6 and GST-E7 were incubated with the whole cell extracts prepared from BJAB cells that expressed endogenous IFI16, and the IFI16 detained by GST fusion proteins were detected using Western Blotting assay with anti-IFI16 antibodies.
     方法:(1)体外GST阻滞分析:克隆、表达及纯化GST鄄HPV16E6和GST鄄HPV16E7融合蛋白,将含有IFI16蛋白的EB病毒阴性伯基特淋巴瘤穴BJAB雪细胞提取物与上述纯化蛋白孵育,通过WesternBlotting分析蛋白质的体外相互作用;
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     The fusion proteins GST-ERβ-AF1and His-tagged candidate protein were purified through affinity column. The GST pull-down assay with the purified proteins further confirmed the interaction.
     然后将ERβ-AF1和候选蛋白分别构建到pGEX-KG和pET-28 a上,并纯化GST-ERβ-AF1和H is-候选蛋白融合蛋白,利用GST沉淀实验进一步验证ERβ-AF1和候选蛋白的体外相互作用
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     Fusion expression of C17orf25 and GFP and computer analysis showed that C17orf25 was probably located in mitochondria.
     之后的NTA Ni2 + 亲和柱层析实验表明这两种蛋白质在体外相互作用。 将C17orf2 5与绿色荧光蛋白基因在SMMC772 1中融合表达 ,结果表明C17orf2 5蛋白可能定位在线粒体中 ,侧面印证了在细胞内与NUDT9作用的空间可能性。
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  相似匹配句对
     In vitro interaction of Cryptococcus neoformans and keratinocyte
     隐球菌与体外培养角质形成细胞的相互作用
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     The Interaction of Calcium Phosphate Biomaterials with Proteins in Vitro and Vivo
     体内及体外磷酸钙与蛋白质生物材料相互作用
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     Interaction of antimatters
     反物质的相互作用
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     2.the mechnisms of interaction;
     相互作用的机制;
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     Herniotomy was accomplished with sledge needle and extracorporeal knotting.
     体外打结。
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  interaction in vitro
IgG immunecomplex interaction in vitro seems to be a potent tool to change the different functional stages.
      
The recombinant proteins were characterized by spectroscopic methods and their interaction in vitro confirmed by pull-down assays.
      
The purpose of the present study was to confirm this interaction in vitro.
      
An antagonistic interaction in vitro between trastuzumab and 5-fluorouracil (5-FUra) in combination has previously been reported.
      
The relationship of the interaction in vitro to the known association of the two organelles in vivo is considered.
      
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The GST gene fusion system was used to express PHO2 gene and its mutants in E. coli. Gel retardation assays showed that PHO2 fusion protein can bind to the upstream activation sequence (UAS) of the acid phosphatase gene PRO5. The homeodomain of PHO2 protein has such structure as α-helix 2-β-turn-α-helix 3,which acts as the DNA binding domain of the transcriptional factor. The Mutation of 11e 123 to Pro in helix 3 or the insertion of 4 amino acids (PDPD)between 112 and 113 in α-helix 2 led to the complete loss...

The GST gene fusion system was used to express PHO2 gene and its mutants in E. coli. Gel retardation assays showed that PHO2 fusion protein can bind to the upstream activation sequence (UAS) of the acid phosphatase gene PRO5. The homeodomain of PHO2 protein has such structure as α-helix 2-β-turn-α-helix 3,which acts as the DNA binding domain of the transcriptional factor. The Mutation of 11e 123 to Pro in helix 3 or the insertion of 4 amino acids (PDPD)between 112 and 113 in α-helix 2 led to the complete loss of DNA-binding activity of PHO2, while the mutation of Pro 117 to Ala in β-turn did not affect the binding activity significantly. Deletions of the PRO80 homologous region,acidic region or C-terminal 182 residues had no great effect on the DNA-binding activity, although these mutants had lost the ability to activate PHO5 expression in vivo.

酵母PHO2蛋白及其变异体与PHO5USA体外的相互作用杨军,敖世洲(中国科学院上海生物化学研究所分子生物学国家重点实验室,200031)关键词酵母;PHO2;突变;DNA结合PHO2是酵母阻遏型酸性磷酸酯酶基因转录的正调控因子[1],由559个氨基...

The Pneumocystis carinii (PC) were isolated through bronchial alveolar lavage from PC pneumonia rats prepared by induction with prednisolone, and it was treated and purified with antiratcommon leukocyte antigen monocolonal antibody. The ingesting ability of macrophage for PC and the effect of glucocorticoid were studied in vitro. The results showed that the PC could be ingested and degraded by peritoneal macrophage (PM φ) and alveolar macrophage (AM φ). The difference of uptake of mature cyst forms (MCFs)...

The Pneumocystis carinii (PC) were isolated through bronchial alveolar lavage from PC pneumonia rats prepared by induction with prednisolone, and it was treated and purified with antiratcommon leukocyte antigen monocolonal antibody. The ingesting ability of macrophage for PC and the effect of glucocorticoid were studied in vitro. The results showed that the PC could be ingested and degraded by peritoneal macrophage (PM φ) and alveolar macrophage (AM φ). The difference of uptake of mature cyst forms (MCFs) of PC between PM φ and AM φ was not significant(P>0.05). The ingesting ability of AM φ for PC in dexamethasone pretreatment group was inhibited after 60 min of phagocytosis in comparison with nonpretreatment group (the uptake of MCFs of PC:10.08±0.39/100M φ vs 12.25±0.28/100M φ,P<0.05). The results suggested that the induction of PC pneumonia in rats with the glucocorticoid may be related to the inhibition of macrophage ingestion ability, and that macrophage may play an important role in the protection of the pathogenesis of PC.

应用支气管肺泡灌洗法从诱导成功的卡氏肺孢子虫(PC)肺炎大鼠中分离PC虫体后,经抗大鼠白细胞共同抗原单克隆抗体(AntiCLAAb)处理使PC高度纯化,然后观察PC与小鼠和大鼠的巨噬细胞在体外的相互作用。结果显示,腹腔巨噬细胞(PMφ)和肺泡巨噬细胞(AMφ)能吞噬并降解PC虫体,PMφ和AMφ与PC相互作用后每100个Mφ吞噬成熟PC包囊数分别为5.80±0.70和4.70±0.51(P>0.05,作用30min时)、11.33±0.73和11.50±0.76(P>0.05,作用60min时)。经过地塞米松预处理与未经处理的AMφ,每100个吞噬成熟PC包囊数分别为4.75±0.36和5.42±0.29(30min,P>0.05)、10.08±0.39和12.25±0.28(60min,P<0.05)。这说明糖皮质激素可抑制AMφ的吞噬功能,推测糖皮质激素诱导大鼠产生PC肺炎可能与其抑制AMφ的吞噬功能有关。结果提示:巨噬细胞在防御PC致病方面可能起重要作用

Objective To elucidate the interaction of protease inhibitors and house dust mite allergen in vitro. Methods Nasal secretion was collected by suction from patients with house dust mite nasal allergy, diluted immediately with PBS pH 7.0, mixed with vortex for 10 minutes, and then centrifuged at 2 000 g for 10 minutes. The supernatant was kept at -80℃ for use. Synthetic low molecular weight fluorogenic peptide substrates were applied to evaluate protease activities. Crude mite extract and other proteases...

Objective To elucidate the interaction of protease inhibitors and house dust mite allergen in vitro. Methods Nasal secretion was collected by suction from patients with house dust mite nasal allergy, diluted immediately with PBS pH 7.0, mixed with vortex for 10 minutes, and then centrifuged at 2 000 g for 10 minutes. The supernatant was kept at -80℃ for use. Synthetic low molecular weight fluorogenic peptide substrates were applied to evaluate protease activities. Crude mite extract and other proteases were used, each of them was preincu bated in the presence or absence of nasal fluid or other protease inhibitors at 37℃ for 30 minutes. The intensity of fluorescence of aminomethylcoumarin released from the cleaved substrate was measured. Results Nasal fluid exhibited mild trypsin like and other protease activities. It inhibited the activities of trypsin and trypsin like serine proteases and papain like cysteine proteases in crude mite extract in a dose dependent manner.Conclusions Nasal fluid possibly plays a role in inhibiting penetration of allergen into the nasal mucosa by producing protease inhibitor protease complex in the nasal mucus layer of patients with mite allergy.

目的研究螨变应原的致敏机理,观察室尘螨变应原蛋白酶与人鼻分泌物中蛋白抑制酶在体外的相互作用。方法鼻分泌物经稀释、混匀、离心后,所得上清液低温保存备用。螨变应原和其它各种蛋白酶分别与各种浓度的水解蛋白抑制酶或不同稀释度的鼻分泌物进行前培养,继而与酶基质进行反应。利用荧光光度计测定被蛋白酶裂解的基质的荧光强度。结果鼻分泌物中显示出轻度的胰蛋白酶样和其它蛋白酶活性,其能抑制胰蛋白酶活性,并对螨变应原的胰蛋白酶样丝氨酸蛋白酶活性和木瓜酶样半胱氨酸蛋白酶活性显示抑制作用,并呈现浓度依赖的抑制效果。结论鼻分泌物中的水解蛋白抑制酶可能在防御螨变应原蛋白酶穿透鼻粘膜上皮屏障中发挥作用。

 
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