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培养植株再生
相关语句
  culture plant regeneration
     Studies on Design and Analysis of Orthogonale Experiment With Null Ratio Factor and Tissue Culture,Plant Regeneration in Sweet Potato(Ipomoea Batatas(L.) Lam.)
     零配比正交试验设计分析与甘薯组织培养植株再生体系研究
短句来源
     It is reviewed on protoplast culture plant regeneration, somatic hybridization by protoplast, and protoplast as an acceptor of genetic engineering in this literature.
     对甘薯原生质体培养植株再生、利用原生质进行体细胞杂交和作为遗传工程受体的研究进行了综述。
短句来源
  “培养植株再生”译为未确定词的双语例句
     In order to establish recepto systems of broccoli (Brassica oleracea var. italica) and cabbage (B. oleracea var. capitata)for heriditary operation, plant regenerations from hypocotyl protoplast cultures were investigated.
     为了建立以青花菜(Brassica oleracea var.italica)和甘蓝(B.oleracea var.capitata)原生质体进行遗传操作的受体系统,进行了下胚轴原生质体培养植株再生研究。
短句来源
     Plant regeneration model of cultivar of Brassica campestris ssp. chinensis makino in vitro was studied. After 6 days of inoculation on MS medium with BA2mg/L,NAA0.5mg/L,ABA 0.5mg/L and AgNO 36mg/L,bud primordia appeared from the cut end of cotyledon petioles of Ji mao cai;
     对 2个小白菜品种的离体培养植株再生方式进行组织学观察 ,结果表明 :在含有 Ag NO36mg/ L及ABA0 .5mg/ L的培养基 (MS+BA2 mg/ L+NAA0 .5mg/ L)中 ,鸡毛菜在培养 6d左右 ,矮脚黄在培养8d左右出现明显的芽原基 ,1 0~ 1 2 d左右产生肉眼可见的不定芽 .
短句来源
     Muell and sprout explants which were from cutting plant of Eremophila glabra(R.Br) Ostenf. (sticky green leaf) were studied.
     的子叶和光秃爱沙木Eremophila glabra(sticky green leaf)的扦插苗带芽茎段为外植体进行组织培养植株再生研究。
短句来源
     An efficient and fast method for plant regeneration from petiole explants with cotyledon was developed in Chinese cabbage( Brassica campestris ssp. chinensis Makino). A MS medium with half strength of NH + 4 concentration and supplemented with BAP 2mg/L,NAA 0.45mg/L and AgNO 3 7.5mg/L was found to be optimum for Chinese cabbage in obtaining the high frequency of shoot regeneration.
     选用 7个白菜类蔬菜栽培品种和 1个杂交亲本进行离体培养植株再生试验 ,筛选出最佳培养基配方为 1/2倍NH+4 浓度的MS培养基附加BAP 2mg/L、NAA 0 .4 5mg/L和AgNO37.5mg/L ,大幅度提高了植株的再生频率 ,高达 84 .8% ,大大缩短成苗周期 ;
短句来源
     In this experiment, systematic study was done on the effect of physiology condition ofexplants, genotype and medium components on plant regeneration of the transgenic plants, 6peanut cultivars were used as source of explants.
     本研究选用 6 个花生栽培品种,研究了外植体类型、生理状态、基因型、培养基激素等因素对花生离体培养再生的影响,明确了器官发生途径、胚胎发生途径的技术条件和再生效率,建立了高效的花生叶片离体培养植株再生体系。
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  相似匹配句对
     Plant Regeneration of Adventitious Roots Culture of Ipomoea batatas
     甘薯不定根培养植株再生
短句来源
     TISSURE CULTURE AND PLANTLET REGENERATION OF AVENA NUDA
     莜麦组织培养再生植株
短句来源
     TISSURE CULTURE AND PLANTLET REGENERATION OF RIMULA MLACOIDES
     报春花的组织培养植株再生
短句来源
     TISSUE CULTURE AND PLANT REGENERATION OFCHENESE CHEVE
     韭菜花序培养植株再生
短句来源
     Tissue Culture and Plantlet Regeneration of Sechium edule
     佛手瓜的组织培养植株再生
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The total 645 immature embruos (after 14 days pollination) from 26 wheat (Triticum aestivum, 2n=6x=42) varieties(lines)crossed with Tripsacum dactyloides (2n=4x=72) were cultured on a hormone-free medium. The results indicated that there were no immediat relations between the frequencies of plant regeneration of cultured embryos and wheat genotypes from which the embryos originated. Well-differentiated immature embryos could speedily germinated and developed into plantlets on the hormone-free medium, while differentiation...

The total 645 immature embruos (after 14 days pollination) from 26 wheat (Triticum aestivum, 2n=6x=42) varieties(lines)crossed with Tripsacum dactyloides (2n=4x=72) were cultured on a hormone-free medium. The results indicated that there were no immediat relations between the frequencies of plant regeneration of cultured embryos and wheat genotypes from which the embryos originated. Well-differentiated immature embryos could speedily germinated and developed into plantlets on the hormone-free medium, while differentiation process of incomplete-differentiated small embryos could not be continued on the same medium. They grew only codeopeiloor, coleopcilc and radicies, and even failed to germinate. Afterwards those cultures with coleopcile or radicles and coleopcilc were transferred on an embryo differentiation medium containing 0.5mg/L KT and 0.5mg/L IAA,their differentiation processes were past remedy. The problems about immature embryo development and regeneration were also discussed.This subject supported by key research subjects of The Chinese Academy of Sciences.

培养了由26个小麦(Triticumaestivum,2n=6x=42)品种(系)用鸭茅状摩擦禾(Tripsacumdactyloides,2n=4x=72)的花粉授粉14天后获得的645个未成熟的胚。结果表明,未成熟胚培养的植株再生频率与胚来源的小麦基因型的杂交亲和性无关。胚的发育程度直接影响胚培养的结果。离体时分化完全的未成熟胚在无激素的培养基上可以迅速萌发成苗,而分化未完全的小胚在无激素的培养基上分化进程不能继续,而且在无激素补充的情况下,萌发过程一旦起动,即使将这些胚转至补加了激素的胚分化培养基上,分化过程也不能再补救。讨论了提高未成熟胚培养成苗率的关键和措施。

In order to establish recepto systems of broccoli (Brassica oleracea var. italica) and cabbage (B. oleracea var. capitata)for heriditary operation, plant regenerations from hypocotyl protoplast cultures were investigated. Hypocotyl protoplasts were isolated from in vitro grown seedings at varying room temperature (20~25℃) under natural illumination. After the purification of protoplasts, both protoplast yields reached 1×106/gFW. The protoplast yield increased by 100% after the aseptic seedlings of cabbage were...

In order to establish recepto systems of broccoli (Brassica oleracea var. italica) and cabbage (B. oleracea var. capitata)for heriditary operation, plant regenerations from hypocotyl protoplast cultures were investigated. Hypocotyl protoplasts were isolated from in vitro grown seedings at varying room temperature (20~25℃) under natural illumination. After the purification of protoplasts, both protoplast yields reached 1×106/gFW. The protoplast yield increased by 100% after the aseptic seedlings of cabbage were pretreated at 4℃ for 6 hours. Using agarose bead culture, the purified protoplasts cultured in K8P medium supplemented with NAA 1. 0 mg . L-1,2,4-D 0. 1 mg.L-1 ZT 0. 5 mg . L-1 and agarose 0. 6%. It was observed that the division frequency of regenerated cells was 35% and the plating efficiency was 5% in broccoli and the plating efficiency was 4% in cabbage. When regenerated calli were transferred onto MS medium supplemented with IAA 0. 1 mg. L-1, ZT 2. 0 mg . L-1,adenine 40 mg . L-1 and glutamine 100 mg . L~1 multiple buds were quickly induced and the differentiation rate was 20% in broccoli and 18% in cabbage. A number of regenerated plantlets were obtained and transplanted and survived.

为了建立以青花菜(Brassica oleracea var.italica)和甘蓝(B.oleracea var.capitata)原生质体进行遗传操作的受体系统,进行了下胚轴原生质体培养植株再生研究。从室温(20~25℃)和自然光照下生长的无菌苗下胚轴游离的原生质体,经纯化后的得率均为1×10~6个·g~(-1)(FW);甘蓝无菌苗经4℃低温处理6h,原生质体产量可增加1倍。采用琼脂糖小块培养(Agarose bead culture)方法,培养在含NAA 1.0mg·L~(-1)、2,4-D0.1mg·L~(-1)和 ZT 0.5mg·L~(-1)的K8P培养基中的原生质体,青花菜再生细胞的分裂频率为35%,植板率为5%;甘蓝的植板率为4%。再生愈伤组织转入含IAA 0.1mg·L~(-1)、ZT 2.0mg·L~(-1)、adenine 40mg·L~(-1)和glutamine 100mg·L~(-1)的MS培养基中可迅速分化出绿芽,青花菜的分化率达20%,甘蓝的为18%。获得了一批再生植株并移栽成活。

In this paper,the achievement of cotton biotechnology studies in China was reviewed,andof the future direction was prospected.

本文综述我国棉花生物技术取得的成就,探讨我国棉花生物技术书来的发展方向,指出棉药组织培养植株再生和体细胞无性系变异的实质问题及遗传转化问题是棉花生物技术未来发展方向中两个不可分割的核心问题。

 
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