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细胞增殖基因
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  cell proliferation gene
     Methods Semi quantitative reverse transcription PCR (RT PCR) was used assay the levels of the accelerating cell proliferation gene Bcl x and the accelerating cell death gene IL 1 beta convetring enzyme (ICE) and the difference between each groups was compared.
     方法 用半定量逆转录PCR(RT PCR)检测细胞凋亡过程中促进细胞增殖基因Bcl x和促进细胞死亡基因白细胞介素 1β转换酶 (interleukin 1βconverten zyme ,ICE)的有效蛋白mRNA的表达水平 ,比较各组的差异。
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  “细胞增殖基因”译为未确定词的双语例句
     Using techniques of cell culture in vitro and RT\|PCR(reverse transcription PCR)gene expressions of p 53, Fas and bcl \|2 of human hepatoma(ATCC,QGY\|7701 line)cells treated with Xinjiang Artemisia flavonoid were analysed.
     应用体外细胞培养及 RT- PCR(reverse transcription PCR)分析了新疆一枝蒿总黄酮类作用后肝癌细胞 (肝癌、ATCC QGY- 770 1 )中凋亡基因 p53、Fas及细胞增殖基因 bcl- 2的表达及它们之间的差异 .
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     Aim To research expressions of from p53,Fas and bcl 2 genes in Hela cells treated with Munziq and Mushil (Uighur Medicine)of abnormal Savda.
     目的研究异常黑胆质的成熟剂及清除剂两种复方作用前后,Hela细胞中凋亡基因p53,Fas及细胞增殖基因bcl2的表达及它们之间的差异。
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     It proved that Danggui buxue tang could increase the bcl-2 and cmy-c mRNA expression of bone marrow cells. So it is concluded that the Danggui buxue tang can promote the proliferation of bone marrow cells and restrain its apoptosis.
     证明当归补血汤能促进骨髓有核细胞抗凋亡作用基因bcl-2和促细胞增殖基因cmy-cmRNA表达显著增加,从而推断当归补血汤能够促进骨髓细胞增殖并抑制其凋亡。
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     To elucidate the mechanism of mitochondrial damage induced by rotenone and the possible biological function of NADH in repairing mitochondrial damage of PC12 cells, cytotoxicity test, immunocytofluorescence and flow cytometric analysis were used to investigate the changes of cell proliferation genes (c myc, c erbB 2), apoptosis inhibition genes bcl 2, p53 tumor suppressor protein, cell immediate early gene (c fos) and proliferating cell nuclear antigen (PCNA) in PC12 cells before and after exposure to rotenone.
     为探讨NADH对PC12细胞线粒体鱼藤酮损伤的修复机制 ,采用细胞毒实验、免疫荧光和流式细胞仪检测细胞在鱼藤酮损伤前后细胞增殖基因 (c myc、c erbB 2 )、抗凋亡基因 (bcl 2 )、抑癌基因 (p5 3)、细胞快反应基因 (c fos)相关蛋白和细胞增殖核抗原 (PCNA)的表达。
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     And increased c-myb expression both at mRNA and protein level (46%, p<0.01), but no effect on WT1 expression in U937. Conclusions: Wilms tumor gene regulates the expression of c-myb gene expression in U937 cells.
     5 ,10 μmol/Lc -myb基因反义脱氧寡核苷酸可抑制其Myb蛋白 39%、83% (与对照组比较 ,均P <0 .0 1) ,对WT1表达无显著改变。 结论 :WT1可以通过下调c -myb表达抑制U937细胞增殖基因表达
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     Based on these results, it is concluded that the introduction of HSS gene into hepatoma cells might be able to stimulate directly the cellular growth in vitro, allowing a possibility of reevaluation of HSS mechanism in intact cells.
     HSS基因表达可促进细胞增殖
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     p53 was correlated with the modulation of proliferation respectively;
     p53基因细胞增殖水平的调控有关。
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     Existence of T Cell Proliferation Factor-like Gene in Carp
     鲤鱼存在T细胞增殖因子类基因(简报)
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     The ALP granul es in the cells stained thicker and th e cells' proliferation cycle shortened.
     细胞增殖周期缩短。
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     Wild-type p53 gene can inhibit cell proliferation and induce apoptosis.
     p53基因具有抑制细胞增殖、启动细胞凋亡作用。
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  cell proliferation gene
The T cell proliferation gene IL7R (CD127) was also underexpressed in PPMS, but was up-regulated in SPMS compared to the controls.
      
The light blue bar on the left indicates the cell proliferation gene expression signature.
      


Expression of C-erbB-2 and bcl-2 protein were detected immunohistochemically in 21 cases of benign pleomorphic adenomas and 24 cases of malignant pleomorphic adenomas of salivany gland. The results showed that C-erbB-2 and bcl-2 were overexpressed in benign pleomorphic adenomas. The positive rates of C-erbB-2 and bcl-2 in benign pleomorphic adenomas were 29% and 19% respectively. The positive staining mainly rested on the cytoplasms of grandular epithelial cells in the tubular structures. The positive rates...

Expression of C-erbB-2 and bcl-2 protein were detected immunohistochemically in 21 cases of benign pleomorphic adenomas and 24 cases of malignant pleomorphic adenomas of salivany gland. The results showed that C-erbB-2 and bcl-2 were overexpressed in benign pleomorphic adenomas. The positive rates of C-erbB-2 and bcl-2 in benign pleomorphic adenomas were 29% and 19% respectively. The positive staining mainly rested on the cytoplasms of grandular epithelial cells in the tubular structures. The positive rates of C-erbB-2 and bcl-2 in malignant pleomorphic adenomas were 58% and 46% respectively . The positive staining of C-erbB-2 protein mainly existed on membrane of adenocarcinoma cells,bcl-2 protein mainly distributed on neclei membrane and cytoplasms of adenocatcinoma cells. neclei membrane and cytoplasms of carcinoma cells. The salivary gland ductal epithelial cells near tumors were positive for C-erbB-2 and bcl-2. The results indicated that overexpression of C-erbB-2 protein played an important role in the tumorigenesis, development and transformation of salivary gland tumors. It further indicated that cell apoptosis is also one of the reasons in the tumorigenesis of salivary gland.

为了探讨细胞增殖和细胞凋亡的调控基因在涎腺肿瘤的发生发展及恶性转变中的作用及其意义。本文采用免疫组化方法对5例瘤旁腺体、21例良性多形性腺瘤和24例恶性多形性腺瘤中C—erbB—2和bcl—2蛋白产物的表达进行形态学观察。结果表示:C—erbB—2和bcl—2在良性多形性腺瘤中均过度表达,表达率分别为29%和19%,主要分布于腺管样结构的腺上皮细胞的胞浆中。恶性多形性腺瘤中C—erbB—2的表达率为58%,主要分布于腺癌细胞的胞膜;bcl—2表达率为46%,主要分布于腺癌细胞的核膜及部分胞浆中。5例瘤旁腺体内导管上皮细胞也存在C—erbB—2和bcl—2的过度表达。结果表明:C—erbB—2蛋白产物的过度表达在涎腺多形性腺瘤的发生发展及恶性转变中起着重要的作用,同时也表明在涎腺多形性腺瘤发生中不仅存在细胞增殖基因调控失常同时也存在着细胞凋亡基因的调控失常,它们协同作用共同参与了涎腺多形性腺瘤的发生发展及恶性转化。

Calponin is a kind of gene that can suppress the proliferation of cells, and it may express abnormally under some pathological conditions. We transferred the human recombinant Calponin (hCN) into the cultured vascular smooth muscle cells (VSMC) of spontaneously hypertensive rats (SHR) with the mediation of liposome + pCAGGS. hCN could be expressed in cultured SHR VSMC for more than 4 weeks. MTT method was used to detect the proliferation of VSMC. The results revealed that the proliferation of cultured SHR...

Calponin is a kind of gene that can suppress the proliferation of cells, and it may express abnormally under some pathological conditions. We transferred the human recombinant Calponin (hCN) into the cultured vascular smooth muscle cells (VSMC) of spontaneously hypertensive rats (SHR) with the mediation of liposome + pCAGGS. hCN could be expressed in cultured SHR VSMC for more than 4 weeks. MTT method was used to detect the proliferation of VSMC. The results revealed that the proliferation of cultured SHR VSMC could be significantly suppressed by the transfer of hCN. It was suggested that Calponin could play a role in the prevention and treatment of re stenosis after angioplasty and in the recovery of vascular wall hyperplasia during hypertension.

Calponin是一种抗细胞增殖基因,在多种病理情况下表达失调。用脂质体+载体质粒pCAGGS作介导向培养的自发性高血压大鼠(SHR)血管平滑肌细胞内导入人重组Calponin(hCN),目的基因可在该细胞组织内表达,并持续较长时间(4周以上)。MTT法的检测结果表明,导入hCN基因能明显抑制SHR平滑肌细胞的增殖。这种作用对血管成形术后再狭窄的防治和高血压时血管壁肥厚的逆转具有潜在的实用价值。

Objective To investigate the effects of sodium fluoride (NaF) on the expression of c Fos and c Jun genes and osteoblast cell proliferation of rat osteoblasts. Methods Osteoblastic cells were isolated from baby rat calvaria, and cultured in the presence of different doses of NaF (10 -5 mol/L, 10 -4 mol/L, and 10 -3 mol/L). Cell proliferation was measured by the MTT method, and c Fos and c Jun expression was detected by the immunohistochemistry combined with image analysis compute system....

Objective To investigate the effects of sodium fluoride (NaF) on the expression of c Fos and c Jun genes and osteoblast cell proliferation of rat osteoblasts. Methods Osteoblastic cells were isolated from baby rat calvaria, and cultured in the presence of different doses of NaF (10 -5 mol/L, 10 -4 mol/L, and 10 -3 mol/L). Cell proliferation was measured by the MTT method, and c Fos and c Jun expression was detected by the immunohistochemistry combined with image analysis compute system. Results Results from the MTT assay showed that NaF increased the proliferation of rat osteoblast and induced the expression of c Fos and c Jun genes. The increases of c Fos and c Jun expression by the 3 different doses of NaF were 5 4%, 15 4% and 42 3% for the c Fos gene and 12 1%, 14 4% and 38 6% for c Jun gene respectively ( P< 0 05 and P< 0 01). Conclusion NaF increased rat osteoblastic cell proliferation and the expression of genes involved in cell proliferation.

目的 探讨氟化钠 (NaF)对离体成骨细胞c Fos、c Jun基因表达的调控及细胞增殖的影响。方法 采用新生大鼠头盖骨分离成骨细胞 ,在不同浓度NaF(10 -5mol/L、10 -4 mol/L、10 -3 mol/L)中进行培养 ,用四甲基偶氮唑蓝比色法检测细胞增殖 ,用免疫组化SP法结合计算机图像处理系统检测NaF诱导的c Fos、c Jun表达。结果 实验表明NaF可刺激成骨细胞增殖 ,并诱导成骨细胞c Fos、c Jun的表达 ,与对照组相比 ,NaF各组增加c Fos、c Jun表达分别为 5 4%、15 4%、42 3%及 12 1%、14 4%、38 6 % (P值分别小于 0 0 5及 0 0 1)。结论 NaF促进成骨细胞增殖基因的表达及细胞的增殖 ,其表现为剂量依赖性

 
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